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Using Lineweaver Burk Equation

The document summarizes using the LineWeaver Burk equation to determine the Michaelis-Menten constant (Km) and maximum reaction velocity (Vm) for an enzyme reaction. It shows that Km is approximately 0.76 and Vm is approximately 3 based on the given values. It then uses another equation to determine that the maximum velocity (Vmax) is 300 and the Michaelis constant (Km) is approximately 2/5. The closest matching values for Km and Vm according to the choices given are 1.89 and 0.55 respectively.

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Diana Bunagan
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17 views2 pages

Using Lineweaver Burk Equation

The document summarizes using the LineWeaver Burk equation to determine the Michaelis-Menten constant (Km) and maximum reaction velocity (Vm) for an enzyme reaction. It shows that Km is approximately 0.76 and Vm is approximately 3 based on the given values. It then uses another equation to determine that the maximum velocity (Vmax) is 300 and the Michaelis constant (Km) is approximately 2/5. The closest matching values for Km and Vm according to the choices given are 1.89 and 0.55 respectively.

Uploaded by

Diana Bunagan
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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1.

2. Using LineWeaver Burk Equation

Km/Vm = .2604

Vm = 1/.3407 = 2.935134 approximately 3

Km= vm*(km/vm) =.2604*2.9351 =.764 approximately 8

K2= vmax/[E] = 3E-06/E-08= 300

Vmax [ S ]
3. 0.12 V = where km∧S are equal
km+S
Vmax [ S ] 6
0.12 V = cancelboth s will give you value of alsoequal ¿ 2/5
2S 25

4. Using Eadie-Hofstee
Km=1.2114

Vm=.4499

Nearest value in the choices is Km=1.89, Vm=.55

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