Hematology Analyzer

BK-6310

Service Manual

BIOBASE GROUP

Version 2021.03
Major warning signs used for the analyzer

Caution

Biochemical hazard

Warning for laser

Warning for high voltage

Warning for puncture

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 Content
目录
I. Analyzer installation................................................................................................................................................... 5
1.1 Analyzer unpacking.........................................................................................................................................5
1.1.1 Unpacking steps........................................................................................................................................... 5
1.1.2 Handling method.......................................................................................................................................... 5
1.2 Installation and environmental requirements.................................................................................................. 5
1.3 Space requirement........................................................................................................................................... 5
1.4 Remove the auxiliary fixing structure for transportation................................................................................ 6
1.5 Connect the reagents and waste barrel............................................................................................................ 6
1.6 Power connection............................................................................................................................................ 7
1.7 Startup............................................................................................................................................................. 7
1.8 Blank Test....................................................................................................................................................... 8
1.9 Software installation and upgrading................................................................................................................8
1.9.1 Installation of operation system for upper machine..................................................................................... 8
1.9.2 Installation of upper computer software.....................................................................................................11
1.9.3 System program installation of main control board................................................................................... 12
Preparation................................................................................................................................................................... 22
Step 1: Firstly, program the quickloader.............................................................................................................22
Step 2: Then update the EBoot............................................................................................................................23
Step 3: Then update the wince kernel................................................................................................................. 23
1.9.4 Application software installation of middle computer...............................................................................24
Master lower computer........................................................................................................................................25
1.9.6 Time sequence installation of fluid path.................................................................................................... 33
II.Hardware system...................................................................................................................................................... 34
2.1 Introduction of boards:.................................................................................................................................. 34
2.1.1 Introduction of industrial control board:.................................................................................................... 34
2.1.2 Introduction of interface board:..................................................................................................................37
2.1.3 Introduction of touch screen control board:............................................................................................... 39
2.1.4 Introduction of DC-DC board:................................................................................................................... 39
2.1.5 Introduction of RFID Reader:.................................................................................................................... 40
2.1.6 Introduction of main control board:........................................................................................................... 41
2.1.7 Introduction of CBC pre-amplifying board:...............................................................................................44
2.1.8 FSC pre-amplifying board:.........................................................................................................................47
2.1.9 FLC pre-amplifying board:........................................................................................................................ 47
2.1.10 SSC pre-amplifying board:.......................................................................................................................48
2.1.11 Laser driver board:................................................................................................................................... 49
2.1.12 LED display board:.................................................................................................................................. 50
2.1.13 Driver board............................................................................................................................................. 51
2.1.14 Optocoupler detection board:................................................................................................................... 56
2.2 Location of each board in the analyzer......................................................................................................... 57
2.3 Wires interconnection diagram..................................................................................................................... 59
III.Software system...................................................................................................................................................... 65
3.1 Software architecture.....................................................................................................................................65
3.2 Software functions of upper computer.......................................................................................................... 65
IV.Fluid path system.................................................................................................................................................... 68
4.1 Schematic of fluid path................................................................................................................................. 68
4.2 Composition of fluid path system................................................................................................................. 68
4.2.1 Electromagnetic valve................................................................................................................................ 68
4.2.2 Syringe....................................................................................................................................................... 69
4.2.3 Other auxiliary devices...............................................................................................................................69
4.3 Analyzer test procedures............................................................................................................................... 70
4.3.1 CBC+DIFF test procedure in whole blood mode.......................................................................................70
4.3.2 CBC test procedure in whole blood mode................................................................................................. 70
4.3.3 CBC+DIFF measurement procedure in pre-diluted mode......................................................................... 71

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 4.3.4 CBC test procedure in pre-diluted mode....................................................................................................71
4.4 Instructions of CBC+DIFF whole blood measurement process....................................................................71
4.4.1 Sample aspiration by sampling probe (0~3S)............................................................................................ 71
4.4.2 Test procedure for DIFF channel............................................................................................................... 71
4.4.3 Measurement procedure for CBC channel................................................................................................. 73
4.5 Process instructions of time sequence for other measurements.................................................................... 75
4.5.1 Process instructions of time sequence for CBC whole blood measurement.............................................. 75
4.5.2 Process instructions of time sequence for CBC+DIFF pre-dilution measurement.................................... 75
4.5.3 Process instructions of time sequence for CBC pre-dilution measurement............................................... 75
4.5.4 Others......................................................................................................................................................... 76
4.6 Functional descriptions of fluid path.............................................................................................................76
V.Mechanical system................................................................................................................................................... 82
5.1 Host structure................................................................................................................................................ 82
5.1.1 Main panels structure of the analyzer........................................................................................................ 82
5.2 Removal and installation of host...................................................................................................................85
5.2.1 Opening and right-side doors..................................................................................................................... 85
5.2.2 Opening of front face shell.........................................................................................................................87
5.2.3 Removal and installation of top plate.........................................................................................................87
5.2.4 Removal and installation of industrial control board (for BK-6310 model only)......................................88
5.2.5 Removal of cooling fan, main control board and CBC pre-amplifying board...........................................90
5.2.6 Removal and installation of driver board...................................................................................................90
5.2.7 Removal and installation of hard disk........................................................................................................92
5.2.8 Removal and installation of RFID assembly..............................................................................................93
5.2.9 Replacement of fuse................................................................................................................................... 93
5.2.10 Removal and installation of power supply assembly............................................................................... 94
5.2.11 Removal of counting chamber assembly................................................................................................. 95
5.2.12 Removal and installation of DIFF chamber assembly............................................................................. 96
5.2.13 Removal and installation of sampling assembly and sampling probe..................................................... 97
5.2.14 Removal and installation of waste fluid pump assembly.........................................................................99
5.2.15 Removal and installation of syringe assembly.......................................................................................100
5.2.16 Removal and installation of electromagnetic valve............................................................................... 100
5.2.17 Removal of hydraulic sensor..................................................................................................................102
5.2.18 Removal of level sensor assembly......................................................................................................... 102
5.2.19 Removal and installation of optical path assembly................................................................................104
5.2.20 Removal and installation of board card in the optical assembly............................................................104
5.2.21 Replace the flow cell.............................................................................................................................. 108
VI.Parameter adjustment setting................................................................................................................................ 112
6.1 Gain adjustment...........................................................................................................................................112
6.1.1 Conditions for gain adjustment................................................................................................................ 112
6.1.2 CBC gain adjustment............................................................................................................................... 112
6.2 Adjustment of mechanical position.............................................................................................................114
6.2.1 Conditions for adjustment of mechanical position...................................................................................114
6.2.2 Mechanical debug.................................................................................................................................... 114
6.3 Adjustment of sensor alarm value...............................................................................................................115
6.3.1 Conditions for adjustment of sensor alarm value.....................................................................................115
6.3.2Adjustment of sensor alarm value.............................................................................................................115
6.4 Pressure adjustment.....................................................................................................................................116
6.4.1 Conditions for pressure adjustment..........................................................................................................116
6.4.2 Pressure adjustment..................................................................................................................................116
6.4.3 Temperature calibration of DIFF chamber...............................................................................................116
6.4.4 Conditions for temperature calibration of DIFF chamber........................................................................116
6.4.5 Temperature calibration of DIFF chamber...............................................................................................116
6.4.6 Export and import of delivery configuration parameters......................................................................... 117
6.5 Test.............................................................................................................................................................. 117
6.5.1 Functional test.......................................................................................................................................... 117
6.5.2 Performance test.......................................................................................................................................118
VII.Maintenance and Correct erroring.......................................................................................................................120

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7.1 Analyzer maintenance.................................................................................................................................120
7.1.1 Daily maintenance....................................................................................................................................120
7.1.2 Weekly maintenance................................................................................................................................ 120
7.1.3 Monthly maintenance...............................................................................................................................120
7.1.4 Other maintenance....................................................................................................................................120
7.2 Fault handling..............................................................................................................................................121
7.2.1 Clinical alarm........................................................................................................................................... 121
7.2.2 Fault Alarm.............................................................................................................................................. 124

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 I. Analyzer installation
1.1 Analyzer unpacking
1.1.1 Unpacking steps
Unpack the analyzer, and properly store the packing case and the packing materials for convenience
of repacking analyzer in the future.
1.Keep the package upright, and make sure the package arrow up.
Unpack the packing case with a tool, take out the accessories, and check whether the parts are
complete according to the packing list
2.Remove the instructions and the accessories from the buffering sponge, and keep them properly for
use in the future. Take out the upper buffering sponge, remove the damp-proof plastic bag cased on
the Analyzer, then lift the analyzer out of the packing case carefully by at least two persons who hold
the hand-lifting positions at the bottom on both sides of the analyzer, and place it on a horizontal
bench.
Notice: The packed accessories of the product shall be subject to the packing list.
1.1.2 Handling method
●Before handling, make sure to bind the sampling assembly reliably, in particular to ensure the
sampling probe in the topmost position.
●Under the condition of a short distance, use a trolley or other transport machines for transportation.
●In all the processes of handling and transportation, pay attention to protect the front panel and
sampling probe, and keep the fluid path on the back panel from bearing external force, contacting
with other object, and being damaged.
●In all the processes of handling and transportation, the machine must be kept in the upright
direction, the inclination angle shall not be greater than 15°, and the machine can’t be placed laterally.
●In handling, try to keep steady and avoid vibration, and firstly perform inspection and debugging
after handling before application.
1.2 Installation and environmental requirements
The analyzer must be installed by authorized personnel. In order to ensure the normal operation of
the analyzer, please select the working place meeting the requirements below to place the analyzer:
Range of operating temperature range: 15 ℃~30 ℃
Range of operating humidity range: 30%~85%
Range of operating atmospheric pressure: 70 kPa~106 kPa
The environment shall be free from dust, mechanical vibration, pollution, big noise source and power
interference as much as possible.
It is recommended to evaluate the electromagnetic environment of the lab before operating the
equipment.
Never get the equipment close to any strong electromagnetic interference source, so as to avoid the
influence on the normal operation of the equipment.
Never get close to any brush motor, flashing fluorescent lamp, and frequently on-off electric contact
equipment.
Avoid direct sunshine or placing it in front of any heat source and wind source.
Select a well-ventilated position.
Never place the main machine on an inclined plane.
1.3 Space requirement
The reserved space between the right door of the main machine and the wall shall be
≥100cm, the reserved space between the back panel of the main machine and the wall shall be≥50cm,
and enough space shall be ensured below the operating bench and the main machine for placing the
buckets of diluents and waste fluid (the space necessary for maintenance and service shall be ensured,
the heat radiation of the analyzer is considered, and the fluid pipelines behind the main machine are
not extruded to affect the normal flowing of reagents).

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1.4 Remove the auxiliary fixing structure for transportation
Remove the binding wires of the movable mechanism on the sampling assembly; binding wires are
available in two positions, respectively.

1.5 Connect the reagents and waste barrel

1. Connect the reagent and waste fluid pipes to the reagent bottle and the corresponding interfaces
on the back panel of BK-6310 analyzer. The interfaces are as shown in the figure below.

Picture of lower left interface on the back of the machine

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 Picture for connecting the machine side to reagent

Table 1- 1 Reagent Interface Connection list

Interface Reagent Machine interface Interface color

1 Diluent W-61D Diluent Blue

2 Waste Waste Black
3 Waste sensor W. sensor
4 Lyse W-61LD LD Lyse Green
5 Lyse W-61LH LH Lyse Red
Probe cleanser W- Directly aspirate from the sampling
5P probe

1.6 Power connection

1. Connect the BK-6310 analyzer with a patch board or socket with a power cord.
2.Connect the printer with BK-6310.
1.7 Startup
After confirming that all the connections related to the analyzer are correct, firstly switch on the
power socket, and then turn on the power switch of the BK-6310 analyzer. After the analyzer
displays the login interface, input the user name of “wmserv” and input the corresponding password,
press “OK” to enter the interface of waiting for connection, and enter the report interface on
completion of initiation, perfusion, cleaning and blank best.

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1.8 Blank Test
1. Blank test

Click “ → ” on the software interface, and select the blank test mode as shown in
the figure below:

Then press the count start key for blank test; if the blank fails to reach the standard, perform the

blank test again; if it still fails to reach the standard, enter “ →clean” for “clean fluid
circuit”, and then perform the blank test again until the blank meets the requirements shown in the
table below.
Table 1- 2 Blank Indicators
Parameter Blank value
WBC ≤0.2ⅹ109/L

RBC ≤0.02ⅹ1012/L

PLT ≤10ⅹ109/L

HGB ≤1g/L
HCT ≤0.5%
1.9 Software installation and upgrading
1.9.1 Installation of operation system for upper machine
1.Prepare a USB startup disk and the mobile HDD in which the GHOST system applicable for the
analyzer is copied
2.Start the equipment
3.When LOGO is displayed on the screen, press the “DEL” key to enter the BOIS setup interface
4.Set the “SATA” mode as “AHCI” in “Advanced SATA Configuration”
5.Confirm that the following settings have been correctly set in “Chipset North Bridge”
Boot Display Device [LVDS]
Active LFP [LVDS]
Flat Panel Type [1366x768 LVDS]

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6.Confirm that “Show Full Log” is set as “Disabled” in “BOOT”
7.Save and exit from BOIS setup
8.When LOGO is displayed after restart, press the “F11” key, select “General USB Flash Disk 1.0”
in the interface below and press the enter key

9.Enter the startup interface, select “1” to run “WIN8PEx86 Lite”

10.On completion of WIN8PE startup, start “ghost”, and use the function of “Local Disk From
Image”
11.Select the system image file (disk.gho), then select a local disk (with the volume of 500G), and
start to lead in after a series of confirmations. Then, after waiting for 20-30minutes, the software will
prompt it has been finished. It is unnecessary to restart the system at once. Exit from ghost.
12.Start “DiskGenius”, in the menu of “Hard Disk reconstruct main boot record (MBR)”, and
close the software after success.
13.The WIN7operation system can start normally after restart.
14.After the system starts up, delete the automatically installed 360 Security and 360 Browser under
“All Programs 360 Safe” after startup; if such two kinds of software do not exist, skip the step.
15.Run “AOMEI OneKey Recovery”, and click “OneKey System Backup”

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16.Use default to select “Backup system to AOMEI OneKey Recovery Partition”, and click “Next”

17. Click “Start Backup”

18.The backup takes about 15-20 minutes, and the backup is completed when the following interface
is displayed

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1.9.2 Installation of upper computer software
1.Plug the U disk in which the installation software for the upper computer is copied into the USB
interface of the analyzer or the computer;
2.After entering the catalog of the installation software, click “SETUP.EXE” of the installation
software;

3.Select the installation catalog as “D:\BK-6310 Analyzer”, and then click “NEXT”

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4.Click “Install” to complete the installation

1.9.3 System program installation of main control board

1.9.3.1 FPGA programming
Programmer software installation and FPGA programming procedure
1.1.1 Install the Quartus II programmer software
In the WINDOWS 7 environment of PC, install the installation software under the defaulted disk C:
QuartusProgrammerSetup-13.0.0.156.exe; The installation procedure is as below: Double click on
the icon

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 Double click

Click

Click Next.

Click Next.

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 Use the defaulted

Click

Click Next.

Click

Click Next.

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 Click

Click Finish.
1.1.2 Confirm that the driver software is successfully installed
After successfully installing the Quartus II programmer software, plug the USB plug
of the downloader cable to the USB Receptacle for the PC; the red power light of the downloader
will light up.

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 USB port

USB-Blaster

After the downloader cable is plugged, the device manager on the PC will prompt that the driver is
not installed successfully. Update the driver as below:

Select “Attribute” using the right

mouse button

Click USB-Blaster attribute,

Click Update Driver…

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Then click Browse my computer for driver software

According to the path: C:\altera\13.0\qprogrammer\drivers\usb-blaster Tick the included subfolder at

the same time
Click Next.

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Click Install.

Click Close.

In this way, it shall be then observed on the device manager; if it displays that Altera USB-Blaster

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has no yellow warning sign, it indicates successful installation.
1.1.3 Programming procedure
1. In the shutdown status of the analyzer, firstly remove the left door of the analyzer, and then
remove the upper cover.
2. Then, in the shutdown status of the analyzer, plug the other plug 5PIN*2 (the black line
corresponding to the triangular symbol indicates Pin 1) of the downloader cable to the main control
board receptacle JP2 in the analyzer, and make sure to plug the Pin 1 on the downloader cable to the
Pin 1 (indicated by the triangular symbol) on the receptacle JP2 correspondingly, otherwise the board
card will be burnt. (picture), after it is confirmed without error, turn on the power switch of the
analyzer to power it on.
3. Then click the icon of Quartus II 13.0 Programmer in the start menu on the PC,

Start the Quartus II programmer software, in this interface,

Click Hardware Setup…

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Select USB-Blaster(USB-0), and then click Close, Mode: Active Serial Programming
Click Add File…, find out the file to be downloaded, and lead in the programming file:
DATA_SP1_V21.POF.

Select DATA_SP1_V21, click Open,

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then tick the three items of Program/Configure, Verify, Blank-Check, with the others not ticked.

Then, click Start,

for about 3 minutes,

It prompts successful download.

1.Finally, after the analyzer is powered off normally, unplug the plug 5PIN*2 and USB plug of the
downloader cable, firstly install the upper cover, and then install the left rear door of the analyzer; at
that time, the FPGA programming is finished.
1.9.3.1Operation system installation of main control board

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Preparation
Connect the main control board (UART5) with the PC with a serial port line, and connect the
network card of the main control board to the PC with a cable at the same time.

UART5

Reset key

Step 1: Firstly, program the quickloader

1.Run lpc32x0Loader.exe, and click “Browse” to select the quickload.hex file;

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2.Click the Start to connect button of the lpc32x0Loader.exe, and then press the reset key on the
board;
3.When “Hit "space" to enter command mode.” is displayed, press the space key quickly toenter the
command mode;
4. Input the command:
:>nand erase 0 0x10000000 (Enter)
When the software displays “Nand erase done”, it indicates completion.
5. Input the command:
:>sload 0x80000000 (Enter)
The software displays “Data will be load to address 0x80000000. Now waiting Lpc32x0Loader send
file...”
Click the “serial port send file” button, and select the quickload.bin file;
When the software displays “**************** download done ***************”, the sending is
done.
6. Input the command:
:>nandwritebooter 0x80000000 0xf000 (Enter)
When the software displays “Nand write booter done”, the write-in is done.
7.By pressing the reset key, the main control board can start “QuickLoader” in nandflash.
Step 2: Then update the EBoot
1. Run the lpc32x0Loader.exe, choose "directly enter the terminal", click the "start to connect"
button, and then turn on the power switch of the equipment or press the reset key on the main control
board. When “Hit “space” to enter command mode.” is displayed, press the space key quickly to
enter the command mode;
2. Input the command:
:>sload 0x80000000 (Enter)
The software displays “Data will be load to address 0x80000000. Now waiting Lpc32x0Loader send
file...”
Click the “serial port send file” button, and select EBOOT.nb0;
When the software displays “**************** download done ***************”, the sending is
done.
3. Input the command:
:>nand write 0x80000000 0x40000 0x40000 (Enter)
When the software displays “Nand write done”, the write-in is done.
4. Input the following commands and parameters:
option set AutoCall yes
option set CallDelay 7000
option set CallRunAddr 0x83fc0000 option set CallDataAddr 0x40000 option set CallDataLen
0x40000 option save
When the software displays “NAND writing............”, the write-in is done.
5. The command of option show can be inputted to check whether the parameter has been set
(0x00040000 = 0x40000);
6. Updating ends. Restart the equipment or press the reset key of the main control board.
Step 3: Then update the wince kernel
1.Copy the nk.bin file into the SD card, and insert the SD card into the SD CARD slot of the main
control board;
2.Run the lpc32x0Loader.exe, choose "directly enter the terminal", click the "start to connect" button,
and then turn on the power switch of the equipment or press the reset key on the main control board.
When “Hit space to enter LPC32XX bootloader menu.” is displayed, press the space key quickly to
enter the menu selection;

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3.Select 4. Select the boot device;
4.Then select the submenu 2, SD CARD start mode, and then press Enter;
5.Press 0 to exit from quickloader settings;
6.At this time, the kernel will automatically update on the SD CARD, and start WINCE. Observe
whether the yellow indicator of the network interface of the main control board is on; if it is on, the
WINCE kernel is upgraded correctly.
1.9.4 Application software installation of middle computer
1.9.4.1 Primary software installation
1.Make sure that the cable of the main control board has been correctly connected to the PC, and the
operation system of the main control board has been correctly programmed (after startup, the yellow
indicator for the yellow network interface will light up).
2.Insert the U disk in which the tool software and the application software for the middle computer
are copied into the USB port of the PC, and start the equipment.
3.Start the PC to enter the WINDOWS interface, then confirm that the IP of the PC is the segment of
“192.168.100.XXX”, and start the “LeapFTP” in the U disk.

4.Input “192.168.100.3” in the address file, and then click “Go to”
5.Locate to the catalog of the application software for the middle computer saved on the U disk in
the local catalog
6.Drag the application software of (MidSysNew.exe and para_1.dat) for the middle computer to the
right
7.Restart the equipment or press the reset key of the main control board
1.9.4.2 Application software upgrading of middle computer
1. Log in the system by the permission above administrator, and enter service->software upgrading
to display the following interface:

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2.Choose the software of the middle computer, then click the “upgrade” button, and select the
software (MidSysNew.exe) to be upgraded for the middle computer; the progress bar prompts the
downloading progress; on completion of file downloading, restart the analyzer.

1.9.5 Application software installation of lower computer

Preparation
Confirm whether the computer has installed the “FLASH Programming Utility” in “ Silicon
Laboratories FLASH Programming Utility”; if it is not installed, it is necessary to install the
software at first. The name of installation software is “UtilDLL.exe”
Master lower computer
1.Connect the programmer to the programming interface of the master lower computer, as shown in
the picture below

Programming

2.Connect the USB line of the programmer to the computer

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3.Start “All Programs Silicon Laboratories FLASH Programming Utility”, select the “Debug
Interface” as “JTAG”, and click the “Connect” button, so that the software will prompt Connected.

4.Select the “Download Hex File/Go/Stop” page, click the “Browse” button, choose the application
program (low_master.hex) for the master lower computer, choose “Erase all Code Space before
down”, and click “Download”. Wait the software for prompting successful downloading.

5.Click the “Go” button to start, and it will prompt “Succeeded setting Go”. Then confirm whether
the running indicator flashes normally.

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If it does not flash, reinstall it and carefully make sure that each step is correct. If the problem still
exists, it can be considered that the hardware has a problem.

Running

Slave lower computer 1

1.Connect the programmer to the programming interface of the master lower computer, and link the
jumper wire at the programming port as ”SLV1”, as shown in the picture below

Jumper wire of
programming
interface

Programming
interface

2.Connect the USB line of the programmer to the computer

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3.Start “All Programs Silicon Laboratories FLASH Programming Utility”, select the “Debug
Interface” as “C2”, and click the “Connect” button, so that the software will prompt Connected.

4.Select the “Download Hex File/Go/Stop” page, click the “Browse” button, choose the application
program (low_slv1.hex) for slave lower computer 1, choose the “Erase all Code Space before down”,
and click “Download”. Wait the software for prompting successful downloading.

5.Click the “Go” button to start, and it will prompt “Succeeded setting Go”. Then confirm whether
the running indicator flashes normally.
If it does not flash, reinstall it and carefully make sure that each step is correct. If the problem still
exists, it can be considered that the hardware has a problem.

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 Running
indicator

Slave lower computer 2

1.Connect the programmer to the programming interface of the master lower computer, and link the
jumper wire at the programming port as ”SLV2”, as shown in the picture below

Jumper wire
of
programming

Programmin
g interface
2.Connect the USB line of the programmer to the computer
3.Start “All Programs Silicon Laboratories FLASH Programming Utility”, select the “Debug
Interface” as “C2”, and click the “Connect” button, so that the software will prompt Connected.

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4.Select the “Download Hex File/Go/Stop” page, click the “Browse” button, choose the application
program (low_slv2.hex) for slave lower computer 2, choose the “Erase all Code Space before down”,
and click “Download”. Wait the software for prompting successful downloading.

5.Click the “Go” button to start, and it will prompt “Succeeded setting Go”. Then confirm whether
the running indicator flashes normally.
If it does not flash, reinstall it and carefully make sure that each step is correct. If the problem still
exists, it can be considered that the hardware has a problem.

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 Running
indicator

Slave lower computer 3

1.Connect the programmer to the programming interface of the master lower computer, and link the
jumper wire at the programming port as ”SLV3”, as shown in the picture below

Jumper wire of
programming
interface

Programming
interface

2.Connect the USB line of the programmer to the computer

3.Start “All Programs Silicon Laboratories FLASH Programming Utility”, select the “Debug
Interface” as “C2”, and click the “Connect” button, so that the software will prompt Connected.

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4.Select the “Download Hex File/Go/Stop” page, click the “Browse” button, choose the
temperature control application program (tempCrl.hex), choose the “Erase all Code Space before
down”, and click “Download”. Wait the software for prompting successful downloading.

5.Click the “Go” button to start, and it will prompt “Succeeded setting Go”. Then confirm whether
the running indicator flashes normally.
If it does not flash, reinstall it and carefully make sure that each step is correct. If the problem still
exists, it can be considered that the hardware has a problem.

32
 Running
indicator

1.9.6 Time sequence installation of fluid path

1.Log in the system by the permission above administrator, and enter service->software upgrading to
display the following interface:

2.Choose the series software, then click the “upgrade” button, and select the series software
(tmXXX.bin) to be upgraded; the progress bar prompts the downloading progress until completion of
file downloading.

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 II.Hardware system
From the view of system, the hardware system is between the software system and the mechanical
driving system of the optical path and fluid path, and it plays a role as a connecting link. The
ultimate user manages the whole resources of the analyzer through the software of the upper
computer. Specifically, the software performs the related actions and data acquisition through the
hardware. The specific schematic block diagram of the hardware is as below:

2.1 Introduction of boards:

Based on the design principle, the boards of the hardware system classified into:
 Upper computer related boards: a total 4+1 (optional) types, including industrial control board,
interface board, touch screen control board, DC-DC board and RFID reader;
 Middle computer related boards: 7 types of main control board, CBC pre-amplifying board, FSC
pre-amplifying board, FLC pre-amplifying board, SSC pre-amplifying board, laser driver board and
LED display board;
 Lower computer related boards: 2 types of driver board and optocoupler detection board.
2.1.1 Introduction of industrial control board:
The industrial control board serves as the upper computer, and, it is used as the entire data processing
unit for externally connecting with the printer, mouse and keyboard, for mobile data storage, etc.;

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 USB_LAN USB_LAN1
PCBA layout:
PWR1

SATA1

PWROUT

LVD
J5

SO-DIMM

USB56

Definition of interface signal:

Position Name Definition
PWR1 +12V power input 1 Reference ground
2 Reference ground
3 +12V
4 +12V
LVDS 1 Power supply +3.3V
2 Power supply +3.3V
3 Reference ground
4 Reference ground
5 LCD data set 0 send-
6 EDID interface communication
data
7 LCD data set 0 send+

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 8 EDID interface communication
clock
9 Reference ground
10 Reference ground
11 LCD data set 1 send-
12 LCD clock output-
13 LCD data set 1 send+
14 LCD clock output+
15 Reference ground
16 Reference ground
17 LCD data set 2 send-
18 LCD data set 3 send-
19 LCD data set 2 send+
20 LCD data set 3 send-
J5 LVDS backlight control 1 +12V
interface 2 backlight enable signal
3 Reference ground
4 Backlight highlight control
5 +5V
USB56 1 +5V
2 Reference ground
3 USB5 data-
4 Reference ground
5 USB5 data+
6 USB6 data+
7 Reference ground
8 USB6 data-
9 Reference ground
10 +5V
SATA1 1 Reference ground
2 Data sending+
3 Data sending-
4 Reference ground
5 Data receiving-
6 Data receiving+
7 Reference ground
PWROUT 1 +12V
2 Reference ground
3 VCC
4 Reference ground
5 VCC3

36
 USB_LAN1 Standard USB interface 1 +5V

2 USB data-
3 USB data+
4 Reference ground
USB_LAN2 Standard USB interface 1 +5V
2 USB data-
3 USB data+
4 Reference ground
SO-DIMM Memory slot -- --

2.1.2 Introduction of interface board:

According to the requirements for mechanical design, EMC design, appearance, etc., the purpose for
interface board design only satisfy the physical extension of interface for the industrial control board.
PCBA layout:

J3 J4 J5

J1

J2

Definition of interface signal:

Positio Name Definition

n
J1 Industrial control board 1 Differential signal sending+
network signal switchover 2 Differential signal sending-
receptacle
3 Reference ground
4 Differential signal receiving+
5 Differential signal receiving-
J2 Industrial control board USB1- 1 Power supply +5V
4 signal switchover receptacle 2 Differential signal USB1 data-
3 Power supply ground
4 Differential signal USB1 data+
5 Power supply +5V
6 Differential signal USB2 data-
7 Power supply ground
8 Differential signal USB2 data+
9 Power supply +5V

37
 10 Differential signal USB3 data-
11 Power supply ground
12 Differential signal USB3 data+
13 Power supply +5V
14 Differential signal USB4 data-
15 Power supply ground
16 Differential signal USB4 data+
J3 RJ-45 network interface 1 Differential signal sending+
receptacle 2 Differential signal sending-
3 Differential signal receiving+
4 Isolation transformer terminal
5 Isolation transformer terminal

6 Differential signal receiving-

7 Isolation transformer terminal
8 Isolation transformer terminal
9 Blank
10 Blank
11 Chassis ground
12 Chassis ground
J4 USB2.0 1-2 interface 1 USB0 VBUS
receptacle 2 USB0 data-
3 USB0 data+
4 Reference ground
5 Chassis ground 1
6 Chassis ground 2
7 USB1 VBUS
8 USB1 data
9 USB1 data+
10 Reference ground
11 Chassis ground 1
12 Chassis ground 2
J5 USB2.0 3-4 interface 1 USB2 VBUS
receptacle 2 USB2 data-
3 USB2 data+
4 Reference ground
5 Chassis ground 1
6 Chassis ground 2
7 USB3 VBUS
8 USB3 data-
9 USB3 data+

38
 10 Reference ground
11 Chassis ground 1
12 Chassis ground 2

2.1.3 Introduction of touch screen control board:

Controlled by capacitive screen; connect and communicate with the industrial control board through
the USB5 interface.
PCBA layout:

Positio Name Definition

n
J1 FPC flat cable seat 1 of -- --
capacitive touch screen
J2 FPC flat cable seat 2 of -- --
capacitive touch screen
J3 Touch screen USB 1 Chassis ground EGND
interface 2 Power supply VUSB(+5V)
3 Reference ground GND
4 USB data+USB_DP
5 USB data-USB_DM
Definition of interface signal:
2.1.4 Introduction of DC-DC board:
+24V switchover +12V power supply, provide independent power supply for the industrial control
board; PCBA layout:

39
 J1

J2

Definition of interface signal:

Positio Name Definition
n
J1 +24V power input 1 +24V
2 Reference ground
J2 +12V power output 1 +12V
2 Reference ground

2.1.5 Introduction of RFID Reader:

The Analyzer is a close system, using the RFID Reader to read the card provided together with the
reagents, and it is connected and communicated with the industrial control board through the USB6
interface;
Product picture

Mini-B USB
receptacle

Definition of interface signal:

40
 Position Name Definition
Mini-B USB USB interface 1 V Bus (power supply+5V), red
receptacle communication 2 Data- (data-), white
3 Data+ (data+), green
4 Floating (ID)
5 Reference ground, black

2.1.6 Introduction of main control board:

The main control board as the middle computer of the system, is used as the action command control
and data acquisition center at the front end of the analyzer, and it downloads commands for the lower
computers to execute;

Schematic block diagram:

41
PCBA layout:

J1

J11 J6 J17
J2
J10
J2

J2

J3

Definition of interface signal:

Position Name Definition

J10 Network communication 1 Differential signal sending+

interface 2 Differential signal sending-
3 Blank
4 Reference ground
5 Reference ground
6 Blank
7 Differential signal receiving+
8 Differential signal receiving-
9 Power supply for network
working state LED indicator
10 Network working state LED
indicator
11 Network connected LED indicator
12 Power supply for network
connected LED indicator
13 Reference ground
14 Reference ground

42
 15 Blank
16 Blank
J11 Serial interface 1 Receiving signal
2 Reference ground
3 Sending signal
J6 LED display and buzzer 1 +5V
interface 2 Reference ground
3 Power indicator ON/OFF signal
4 Running indicator ON/OFF signal
5 Warning indicator ON/OFF signal
6 Laser indicator ON/OFF signal
7 Buzzer ON/OFF signal
8 Reference ground
J17 Main control board +5V 1 +5V power input
+24V power input 2 Reference ground
3 +24V power input
4 Reference ground
J1 Laser power supply 1 +12V
2 Reference ground
3 Laser sleep ON/OFF signal
4 laser feedback current signal
5 Reference ground
J2A FSC signal pre-amplification 1 +12V
interface 2 Reference ground
3 -12V
4 FSC signal
5 Reference ground

J2B FLC signal pre-amplification 1 +12V

interface 2 Reference ground
3 -12V
4 FLC signal
5 Reference ground
J2C SSC signal pre-amplification 1 +12V
interface 2 Reference ground
3 -12V
4 SSC signal
5 Reference ground

43
 J3 CBC signal pre-amplification 1 Power supply -12V
interface 2 Power supply +12V
3 Power supply +24V
4 Power supply +24V
5 Reference ground
6 Power supply +5V
7 Reference ground
8 WBC pre-amplification signal
9 Reference ground
10 RBC/PLT pre-amplification signal
11 Reference ground
12 HGB pre-amplification signal
13 Reference ground
14 WBC constant flow source
detection signal
15 Reference ground
16 WBC clogging voltage signal
17 Reference ground
18 RBC/PLT constant flow source
detection signal
19 Reference ground
20 RBC/PLT clogging voltage signal
21 Reference ground
22 WBC chamber switched to
burning mode
23 Reference ground
24 RBC chamber switched to burning
mode
25 Reference ground
26 Constant flow source power
supply +54V ON/OFF signal
27 Reference ground
28 Burning power supply +110V
ON/OFF signal
2.1.7 Introduction of CBC pre-amplifying board:
Acquire WBC, RBC, PLT and HGB signals; adopt the manner of constant flow source; Schematic
block diagram:

44
PCBA layout:

J1

J4 J3 J2
Definition of interface signal:
Positio Name Definition
n
J1 CBC signal 1 Power supply +12V
pre-amplification 2 Power supply -12V
interface 3 Power supply +24V
4 Power supply +24V
5 Power supply +5V
6 Reference ground
7 WBC pre-amplification signal

45
 8 Reference ground
9 RBC/PLT pre-amplification signal
10 Reference ground
11 HGB pre-amplification signal
12 Reference ground
13 WBC constant flow source detection
signal
14 Reference ground
15 WBC clogging voltage signal
16 Reference ground
17 RBC/PLT constant flow source
detection
signal
18 Reference ground
19 RBC/PLT clogging voltage signal
20 Reference ground
21 WBC chamber switched to burning
mode
22 Reference ground
23 RBC chamber switched to burning
mode
24 Reference ground
25 Constant flow source power supply
+54V ON/OFF signal
26 Reference ground
27 Burning power supply +110V ON/OFF
signal
28 Reference ground
J2 WBC jewel hole 1 WBC jewel hole+
interface 2 WBC jewel hole-
3 Reference ground
J3 RBC jewel hole 1 RBC/PLT jewel hole +
interface 2 RBC/PLT jewel hole -
3 Reference ground
J4 HGB interface 1 Light source LED anode
2 Light source LED cathode
3 Detection PD tube anode
4 Detection PD tube cathode
5 Reference ground

46
2.1.8 FSC pre-amplifying board:
Amplify the light signal of forward small angle; PCBA layout:

J1

Definition of interface signal:

Position Name Definition

J1 FSC signal 1 +12V

pre-amplification 2 Reference ground
interface 3 -12V
4 FSC signal
5 Reference ground

2.1.9 FLC pre-amplifying board:

Amplify the light signal of forward large angle; PCBA layout:

47
 J1

Definition of interface signal:

Position Name Definition

J1 FLC signal 1 +12V

pre-amplification 2 Reference ground
interface 3 -12V
4 FLC signal
5 Reference ground

2.1.10 SSC pre-amplifying board:

Amplify the light signal of lateral scattering angle; PCBA layout:

48
 J1
Definition of interface signal:
Position Name Definition

J1 SSC signal pre- 1 +12V

amplification 2 Reference ground
interface 3 -12V
4 SSC signal
5 Reference ground

2.1.11 Laser driver board:

It ensures the steady output of laser light intensity by using automatic power adjustment APD;
PCBA layout:

49
 J1

JP3

Definition of interface signal:

Position Name Definition

J1 Laser power 1 +12V

supply 2 Reference ground
interface 3 Laser sleep ON/OFF signal
4 laser feedback current signal
5 Reference ground
JP3 Laser diode 1 Laser feedback PD cathode
2 Common terminal
3 Laser LD anode

2.1.12 LED display board:

LED display, the green light indicates power supply, the red light indicates warning, the yellow light
indicates running, and the buzzer gives audible warning;
PCBA layout:

50
 J1

Definition of interface signal:

Position Name Definition

J1 LED display and 1 +5V

buzzer interface 2 Reference ground
3 Power indicator ON/OFF signal
4 Running indicator ON/OFF signal
5 Warning indicator ON/OFF signal
6 Laser indicator ON/OFF signal
7 Buzzer ON/OFF signal
8 Reference ground

2.1.13 Driver board

Driving motor, pump, electromagnetic valve and heating element. Detect position optocoupling, air
pressure, fluid, temperature, etc.;
Schematic block diagram:

51
 J22 J15
J14
PCBA layout:

J23
J13 J12
J16 J17 J18
J2

J21

J1

J24

J10 J7
J6

J19
Definition of interface signal:
Position Name Definition
J22 Driver board+5V 1 Power supply +5V
+24V power input 2 Reference ground
3 Power supply +24V
4 Reference ground
5 Reference ground
6 Power supply +24V
J15 pump driving 1 Power supply +24V
2 Signal driving the pump to be on/off
J14 DIFF heating element 1 Power supply +24V
driving 2 Signal driving the heating element to
be on/off
J13 Electromagnetic valves 1 Signal driving the electromagnetic
15-23 driving valve V15 to be on/off
2 Power supply +24V
3 Signal driving the electromagnetic
valve V16 to be on/off
4 Power supply +24V
5 Signal driving the electromagnetic
valve V17 to be on/off

52
 6 Power supply +24V
7 Signal driving the electromagnetic
valve V18 to be on/off
8 Power supply +24V
9 Signal driving the electromagnetic
valve V19 to be on/off

10 Power supply +24V

11 Signal driving the electromagnetic
valve V20 to be on/off
12 Power supply +24V
13 Signal driving the electromagnetic
valve V21 to be on/off
14 Power supply +24V
15 Signal driving the electromagnetic
valve V22 to be on/off
16 Power supply +24V
17 Signal driving the electromagnetic
valve V23 to be on/off
18 Power supply +24V
19 Signal driving the electromagnetic
valve V24 to be on/off
20 Power supply +24V
21 Signal driving the electromagnetic
valve V25 to be on/off
22 Power supply +24V
23 Signal driving the electromagnetic
valve V26 to be on/off
24 Power supply +24V
25 Signal driving the electromagnetic
valve V27 to be on/off
26 Power supply +24V
27 Signal driving the electromagnetic
valve V28 to be on/off
28 Power supply +24V
J12 Electromagnetic valves 1 Signal driving the electromagnetic
1-14 driving valve V1 to be on/off
2 Power supply +24V
3 Signal driving the electromagnetic
valve V2 to be on/off
4 Power supply +24V
5 Signal driving the electromagnetic
valve V3 to be on/off
6 Power supply +24V

53
 7 Signal driving the electromagnetic
valve V4 to be on/off
8 Power supply +24V
9 Signal driving the electromagnetic
valve V5 to be on/off
10 Power supply +24V

11 Signal driving the electromagnetic

valve V6 to be on/off
12 Power supply +24V
13 Signal driving the electromagnetic
valve V7 to be on/off

14 Power supply +24V

15 Signal driving the electromagnetic
valve V8 to be on/off
16 Power supply +24V
17 Signal driving the electromagnetic
valve V9 to be on/off
18 Power supply +24V
19 Signal driving the electromagnetic
valve V10 to be on/off
20 Power supply +24V
21 Signal driving the electromagnetic
valve V11 to be on/off
22 Power supply +24V
23 Signal driving the electromagnetic
valve V12 to be on/off
24 Power supply +24V
25 Signal driving the electromagnetic
valve V13 to be on/off
26 Power supply +24V
27 Signal driving the electromagnetic
valve V14 to be on/off
28 Power supply +24V
J16 HRZ motor driving 1 HRZ motor phase A+
2 HRZ motor phase A-
3 HRZ motor phase B+
4 HRZ motor phase B-
J17 VTC motor driving 1 VTC motor phase A+
2 VTC motor phase A-
3 VTC motor phase B+

54
 4 VTC motor phase B-
J18 SP motor driving 1 SP motor phase A+
2 SP motor phase A -
3 SP motor phase B+
4 SP motor phase B-
J19 DIL motor driving 1 DIL motor phase A+
2 DIL motor phase A -
3 DIL motor phase B+
4 DIL motor phase B-
J21 Motor position 1 HRZ optocoupler LED power supply
detection 2 Reference ground
3 HRZ motor reset signal
4 Reference ground
5 VTC optocoupler LED power supply
6 Reference ground
7 VTC motor reset signal
8 Reference ground

9 SP optocoupler LED power supply

10 Reference ground
11 SP motor reset signal
12 Reference ground
13 DIL optocoupler LED power supply
14 Reference ground
15 DIL motor reset signal
16 Reference ground
J7 Ambient and DIFF 1 Power supply +3.3V
temperature detection 2 Ambient temperature sensor 1-WIRE
communication
3 Reference ground
4 Power supply +3.3V
5 DIFF temperature sensor 1-
WIRE
communication
6 Reference ground
J10 Serial interface 1 Sending signal
2 Reference ground
3 Receiving signal
J6 Waste fluid detection 1 Waste fluid detection (floater)
2 Reference ground
J24 Hydraulic pressure 1 +5V
detection 2 Reference ground

55
 3 Blank
4 Hydraulic pressure detection
(hydraulic sensor)
J1 Fluid detection 1 5D optocoupler LED power supply
2 Reference ground
3 +5V
4 5D reagent fluid detection
5 5H optocoupler LED power supply

6 Reference ground
7 +5V
8 5H reagent fluid detection
9 CLS optocoupler LED power supply
10 Reference ground
11 +5V
12 CLS reagent fluid detection
13 DIL optocoupler LED power supply
14 Reference ground
15 +5V
16 DIL reagent fluid detection
J2 Electric popup switch 1 +5V
driving 2 Signal driving the electric popup
switch to be on/off

J23 Fan driving 1 Fan power supply +24V

2 Reference ground

2.1.14 Optocoupler detection board:

Detect whether the reagent fluid has bubbles or not; PCBA layout:

J1

56
Definition of interface signal:
Position Name Definition

J1 Detection of 1 Anode A at transmitting terminal

optocoupler 2 Cathode K at transmitting terminal
detecting signal
3 Collector C at receiving terminal
4 Emitter E at receiving terminal

2.2 Location of each board in the analyzer

1.SSC pre-amplifying board 2.Laser driver board

3.FLC pre-amplifying board 4. FSC pre-amplifying board
5.Optocoupler detection board

6.CBC pre-amplifying board 7.Main control board

8.Driver board------ 9.Interface board

57
10.Industrial control board

11.Touch screen control board 12.LED display board

13.RFID reader-writer (optional)

58
14.5V switch power supply 15.DC-DC board
16.24V small switch power supply 17.Repositionable switch
18.EMI filter- 19.Equipotential pole
20.24V big switch power supply

2.3 Wires interconnection diagram

59
60
Item Name Interconnection instructions
1 151000-50-01 Live line Repositionable switch---24V big
switch power supply L|5V switch
power supply L|24Vsmall switch
power supply L
2 151000-50-02 Neutral wire Repositionable switch---24V big
switch power supply N|5V switch
power supply N|24Vsmall switch
power supply N
3 151000-50-03 Wire connecting EMI filter EARTH---
receptacle to chassis Connecting
chassis threaded hole
4 151000-50-04 wire connecting power Connecting chassis threaded hole
supply to chassis ---24V big switch power supply E|5V
switch power supply E|24V small
switch power supply E
5 151000-50-05 +5V +24V main control 5V switch power supply +V,-V|24V
board power cord small switch power supply +V,-V ---
driver board J17
6 151000-50-06 +5V +24V power cord of 5V switch power supply +V,-V|24V
driver board big switch power supply +V,-V ---
driver board J22
7 151000-50-08 main control board - Main control board J12--- Driver
serial port line of driver board board J10
8 151000-50-09 main control board – Main control board J3 -
CBC CBC
board flat cable pre-amplifying board J1
9 151000-50-10 LED display control line Main control board J6-LED display
board J1
10 151000-50-11 Suction key line Key --- Suction key line receptacle
11 151000-50-12 Wire from syringe to Connecting chassis threaded hole ---
chassis SP syringe |DIL syringe
12 151000-50-13 Laser drive wire Main control board J1---Laser driver
board J1
13 151000-50-14 Laser device wire laser driver board JP3---Laser device
diode
14 151000-50-15 FSC signal receiving wire Main control board J2A---
FSC
pre-amplifying board J1
15 151000-50-16 FLC signal receiving wire Main control board J2B---
FLC
pre-amplifying board J1

61
16 151000-50-17 SSC signal receiving wire Main control board J2C---
SSC
pre-amplifying board J1
17 151000-50-18 WBC signal receiving CBC pre-amplifying board J2---WBC
wire chamber (jewel hole)
18 151000-50-19 RBC signal receiving CBC pre-amplifying board J3---RBC
wire

chamber (jewel hole)

19 151000-50-20 HGB signal receivingCBC pre-amplifying board J4---WBC
wire chamber (LED A, K|PD A, K)
20 151000-50-21valve line electromagnetic valve ---Valve line
receptacle
21 151000-50-22 Valve changeover flat Driver board J12 --- Plug of valve
cable SV01-SV14 changeover flat cable SV01-SV14
22 151000-50-23 Valve changeover flat Driver board J13 --- Plug of valve
cable SV15-SV28 changeover flat cable SV15-SV28
23 151000-50-24 Pump line Pump ---Pump line receptacle
24 151000-50-25 Pump patch cord Driver board J15--- Plug of pump
patch cord
25 151000-50-26 Heating element wire Heating element --- Receptacle for
heating element wire
26 151000-50-27 Patch cord of heating Driver board J14--- Plug for patch
element cord of heating element
27 151000-50-28 DIFF temperature sensor Temperature sensor --- Receptacle
wire for DIFF temperature sensor wire
28 151000-50-29 Patch cord of temperature Driver board J7--- Plug for patch cord
sensor of DIFF temperature sensor
29 151000-50-30 Motor wire Motor --- Receptacle for motor wire
30 151000-50-31 HRZ Motor patch cord Driver board J16--- Plug of HRZ
motor patch cord
31 151000-50-32 VTC Motor patch cord Driver board J17--- Plug of VTC
motor patch cord
32 151000-50-33 SP motor patch cord Driver board J18--- Plug of SP motor
patch cord
33 151000-50-34 DIL motor patch cord Driver board J19--- Plug of DIL
motor
patch cord
34 151000-50-35 motor position line Optocoupler --- Receptacle for motor
position line
35 151000-50-36 Motor position Driver board J21--- Plug of motor
changeover flat cable position changeover flat cable
36 151000-50-37 Wire of cooling fan Fan --- Receptacle for wire of cooling
fan

62
37 151000-50-38 Wire of hydraulic sensor Hydraulic sensor module ---
Receptacle for wire of hydraulic
sensor
38 151000-50-39 Patch cord of hydraulic Driver board J24--- Plug for patch
sensor cord of hydraulic sensor
39 151000-50-40 Wire of waste fluid sensor Floater sensor --- Receptacle for wire
of waste fluid sensor
40 151000-50-41 Patch cord of waste fluid driver boardJ6--- Plug for wire of

sensor waste fluid sensor

41 151000-50-42 Wire of fluid sensor fluid sensor --- Receptacle for wire of
fluid sensor
42 151000-50-43 Changeover flat cable of Driver board J1--- Plug for
fluid sensor changeover flat cable of fluid sensor
43 151000-50-44 Ground wire of VTC needle frame --- Connecting
VTC chassis threaded hole
needle frame
44 151000-50-45 Wire of live line EMI filter L--- Electric popup switch
changeover switch L
45 151000-50-46 Wire of neutral wire EMI filter N--- Electric popup switch
changeover switch N
46 151000-50-47 Drive wire of electric Electromagnet of electric popup
popup switch switch --- Driver board J2
47 151000-50-50 DC-DC board+24V DC-DC board J1---24V big switch
power power supply +V,-V
input wire
48 151000-50-51All-in-one industrial Industrial control board
control board+12V power input wire PWR1/PWR2--- DC-DC Board J2
49 151000-50-52 Patch cord of all-in-one industrial control board USB_LAN1|
industrial control board USB1-4 USB_LAN2--- Interface board J2
50 151000-50-53 WIRE of all-in-one Receptacle for industrial control
industrial control board USB56 - touch board USB56 --- Touch screen
screen RFID control board J3|RFID reader-writer
Mini-B
USB (optional)
51 151000-50-54 Network cable for main Industrial control board USB_LAN1-
control board of all-in-one industrial -- Main control board J10
control board LAN1
52 151000-50-55 Network patch cord of Industrial control boardUSB_LAN2--
all-in-one industrial control board LAN2 -
Interface board J1
53 151000-50-56 Display line of all-in-one Industrial control board
industrial control board LCD-TFT LVDS|J5---LCD screen CN1
54 151000-50-57 Wire for hard disk SATA Industrial control board SATA1 ---
of Hard disk SATA

63
 all-in-one industrial control board

55 151000-50-58 Power cord for hard disk Industrial control board PWR_OUT --
of all-in-one industrial control board -
Hard disk PWR

64
 III.Software system
3.1 Software architecture
The software architecture is divided into four layers: upper computer, middle computer, master lower
computer and slave lower computer. The lower computers are mainly responsible for the control of
partial time sequences, the execution of control command sent by the upper computer, and the
driving control of mechanical components; they also have the functions of temperature and pressure
detection, etc. The middle computer realizes the functions of storing the time sequence files of the
analyzer, executing the control command sent by the upper computer, controlling partial time
sequences, acquiring signal data, etc. The upper computer is responsible for the operations of user
interaction, operation and control of analyzer running, result display, printing output, etc. Since only
the upper machine computer system is involved in the user interaction, no more instructions will be
given for the systems of the middle computer and the lower computers.
The software architecture is shown in the figure below:

Figure: Software System Architecture Chart

3.2 Software functions of upper computer

Figure: Software Functions and Architecture Chart of Upper Computer

65
Classification of main functional modules:
Module name Functional description
Report Browse the test results, check, modify, print the results,
etc.
Graph review Display the sample information in the form of graph and
sample details; edit the sample results and information;
check, delete and print the sample results. For open
models, sample counting can be started in this interface.
Quality control L-J quality The quality control product is used for
control quality monitoring of the analyzer, L-J
quality control has two modes of whole
blood and pre-dilution, and the operator
is allowed to perform quality control for
all the parameters or several selected
parameters.

X-B quality X-B quality control performs statistical

control analysis according to the actually tested
sample without using the quality control
product. The analyzer performs X-B
quality control for the three parameters
of MCV, MCH and MCHC, and both the

whole blood samples or pre-diluted

samples can be used as the sample
sources.
Quality controlQuality control of X mean value refers to
of X mean that the mane value of two quality
value control tests is used as the final data for
the calculation of quality control.
Quality controlQuality control of X mean value R,
of X mean namely the mean value – range quality
value R control, is an effective method for
judging and predicting whether the
quality condition has any abnormal
fluctuation, and it is complementary with
L-J quality control. Quality control of X
mean value R uses the quality control
product for \quality control analysis.

Calibration Manual The user can directly input the

calibration calibration parameters here.
Calibration The user calculates the calibration
using coefficient after several tests using the
calibrator calibrator

66
 Calibration ofThe user calculates the calibration
fresh blood coefficient after several tests using
multiple fresh blood samples.
Service System maintenance, check of system status
information,
etc.
Setting Set the modifiable items related to daily operation and
analyzer maintenance.
Exit Exit from the system and execute the related operations
of analyzer shutdown.

67
 IV.Fluid path system

4.1 Schematic of fluid path

4.2 Composition of fluid path system

The fluid path system is composed of 22 electromagnetic valves, 1 pinch valve, 2 syringes, 2
counting chambers, 2 isolation chambers, 1 optical flow cell, 1 waste fluid pump, 1 hydraulic sensor,
1 pressure chamber, 1 preheating chamber, 1 sampling probe, etc.
4.2.1 Electromagnetic valve
Types of Name of Types of
electromagnetic schematic electromagnetic Name of schematic
valves valves
Three-way valve SV1 Three-way valve SV15
Two-way valve SV2 Two-way valve SV16
Two-way valve SV3 Two-way valve SV17
Three-way valve SV4 Two-way valve SV18
Two-way valve SV5 Two-way valve SV19
Three-way valve SV6 Two-way valve SV20
Three-way valve SV7 Two-way valve SV21
Three-way valve SV8 Three-way valve SV22
Two-way valve SV11 Three-way valve SV23

68
 Two-way valve SV12 Three-way valve SV24
Three-way valve SV13 Press-off valve PV1(SV25)
Two-way valve SV14

4.2.2 Syringe
Syringe name Application
Sample aspiration and distribution, sample quantification in the 5-
SP syringe part WBC differentiation and counting processes

Quantitative fluid adding to the reaction chamber, formation of

sheath flow in the flow cell, quantitative sample adding of various
Diluent syringe reagents, cleaning of related pipelines of the sampling probe, flow
cell and counting chamber. Sample preparation, assistance the
sample moving

4.2.3 Other auxiliary devices

Name of material Name of diagram Application

form
Waste fluid pump PUMP1 Discharge the waste fluid, and create positive and
negative pressures in the pressure chamber
Isolation chamber Isolation chamber Prevent foaming and backflow of waste fluid; block
external interference signal
Air pressure sensor Air pressure Monitor the air pressure in the pressure chamber
sensor
Hydraulic sensor Hydraulic sensor Monitor the pressure of the sheath flow related
pipelines
Level sensor Level sensor Detect the existence of reagent

Pressure chamber Pressure chamber Store positive pressure and negative pressure

69
4.3 Analyzer test procedures
4.3.1 CBC+DIFF test procedure in whole blood mode

4.3.2 CBC test procedure in whole blood mode

Except that the DIFF chamber channel is not involved in the test, the rest of counting procedures for
the WBC chamber and RBC chamber are the same as those shown in the above figure.

70
4.3.3 CBC+DIFF measurement procedure in pre-diluted mode

4.3.4 CBC test procedure in pre-diluted mode

Except that the DIFF channel is not involved in the test, the rest of test procedures for the WBC
channel and RBC channel are the same as those shown in the above figure.
4.4 Instructions of CBC+DIFF whole blood measurement process
4.4.1 Sample aspiration by sampling probe (0~3S)
The sample syringe aspirate 20ul of blood sample through the sampling probe.
4.4.2 Test procedure for DIFF channel
1.Drain the DIFF chamber: SV20 valve is opened, and the PUMP1 pump drains the fluid in the DIFF
chamber.
2.Aspirate the LD Lyse: SV1, SV5, SV6 and SV24 valves are opened, and the diluent syringe
aspirate the Lyse.
3.Create positive pressure in the pressure chamber: SV18 and SV23 are opened, and the pump is
opened to build the pressure of 6.5KPA in the pressure chamber:
4 .Add fluid to the DIFF chamber (diluent and Lyse):SV1, SV5 and SV6 valves are opened,

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and the diluent syringe adds diluent and Lyse to the DIFF chamber, at the same time, the sampling
probe is moved into the DIFF chamber, and the SP syringe distribute blood to the DIFF chamber;
SV20 and SV22 valves are opened, and the blood samples in the DIFF chamber are mixed by the
bubbles.
5.Prepare samples: SV1, SV3 and SV4 valves are opened, and the diluent syringe aspirate the reacted
and mixed samples from the DIFF chamber to the sample preparation tube, see the figure below.

Sample preparation Forming sheath flow

6.Form the sheath flow: SV1, SV5, SV11 and SV25 valves are opened, and the diluent syringe
injects the diluent into the flow cell at the constant speed, in order to form a steady sheath flow, see
the above figure.
7.Inject the samples quickly: SV3 and SV4 valves are opened, and the diluent syringe injects the
samples in the sample preparation tube quickly into the flow cell at the low speed ， see the figure
below.

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 Quick injection of sample DIFF measurement
8.DIFF measurement: After the samples reaches the laser detection area of the flow cell and becomes
steady, the test begins, see the above figure.
9.Clean the DIFF channel: On completion of DIFF measurement, all the pipelines and device
surfaces in contact with the samples are fully cleaned.
10.Add base solution to the DIFF chamber: On completion of cleaning the DIFF channel, SV1, SV5,
SV6 and SV24 valves are opened, and the diluent syringe injects a certain volume of diluent as the
base solution into the DIFF chamber.
4.4.3 Measurement procedure for CBC channel
1.Drain the RBC chamber: SV17 valve is opened, and the pump drains the RBC chamber, see the
figure below.

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 Drain the RBC chamber Drain the WBC chamber
2.Measure the HGB background: 2S HGB performs background measurement.
3.Drain the WBC chamber: SV16 valve is opened, and the pump drains the WBC chamber, see the
above figure.
4.Add base solution to the WBC chamber: SV1, SV2 and SV7 valve are opened, and the diluent
syringe injects a certain volume of base solution to the WBC chamber.
5.Distribute blood to the WBC chamber: The sampling probe is moved into the bottom of the WBC
chamber, and the sample syringe injects the samples to distribute blood to the chamber; on
completion of blood distribution, the sampling probe is moved to the upper position to clean the
inner and outer walls of the sampling probe; at the same time, SV16 and SV22 valves are opened,
and the samples are mixed by forming.
6.Aspirate the sample after once dilution: On completion of mixing, the sampling probe is moved
into the bottom of the WBC chamber, the sample syringe aspirate a certain volume of sample after
once dilution, after sample suction, the samples are moved
up, and the outer wall of the sampling probe is cleaned.
7.Aspirate the LH Lyse: SV1, SV2, SV7 and SV15 valves are opened, and the diluent syringe
aspirate the Lyse.
8.Create foaming positive pressure: SV18 and SV23 are opened, and the pump is started to create the
pressure of 6.5KPA in the Pressure chamber:
9.Add fluid to the WBC chamber (diluent and Lyse): SdV1, SV2 and SV7 valves are opened, and the
diluent syringe injects a certain volume of diluent and Lyse to the WBC chamber; SV16 and SV22
valves are opened, and the blood samples in the WBC chamber are mixed by forming.
10.Add base solution to the RBC chamber: SV1, SV2 valve are opened, the diluent syringe injects a
certain volume of base solution to the RBC chamber.
11.Add the sample after once dilution to the RBC chamber: The sampling probe is moved into the
RBC chamber, SV1 and SV3 valves are opened, and the diluent syringe injects a certain volume of
diluent after once dilution and samples after once dilution to the RBC chamber; the sampling probe
is moved to the upper position, and the outer wall of the sampling probe is cleaned at the same time;

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SV17 and SV22 valves are opened, and the samples in the RBC chamber are mixed by forming.
12.Create negative pressure: SV19 and SV23 valves are opened, and the pump is started to create the
pressure of -24KPA in the pressure chamber.
13.Count the WBC and RBC/PLT: SV14 valve is opened and opened, and it begins to count and
measure the WBC and RBC/PLT after the fluid flow becomes steady.

14.Measure the HGB: At 1s before the WBC measurement is finished, it begins to measure the value
of HBG samples.
15.Clean the WBC chamber and the RBC chamber: On completion of various tests, the WBC
chamber and the RBC chamber shall be cleaned thoroughly.
16.Add base solution to the WBC chamber and the RBC chamber: SV1, SV2 and SV7 valve are
opened, and the diluent syringe injects a certain volume of diluent as the base solution into the WBC
chamber; SV1 and SV2 valve are opened, and the diluent syringe injects a certain volume of diluent
as the base solution into the RBC chamber.
4.5 Process instructions of time sequence for other measurements
4.5.1 Process instructions of time sequence for CBC whole blood measurement
The difference between the process of time sequence for CBC whole blood measurement and the
process of time sequence for CBC+DIFF whole blood measurement is that: the measurement process
of the time sequence for CBC whole blood measurement does not include the measurement process
of DIFF channel, and thus the aspirated blood volume is reduced.
4.5.2 Process instructions of time sequence for CBC+DIFF pre-dilution measurement
The difference between the process of time sequence for CBC+DIFF pre-dilution measurement and
the process of time sequence for CBC+DIFF whole blood measurement is that: the mixed blood
sample after external dilution is aspirated for the time sequence of CBC+DIFF pre-dilution
measurement, and other processes are generally the same.
4.5.3 Process instructions of time sequence for CBC pre-dilution measurement
The difference between the process of time sequence for CBC pre-dilution measurement and the

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process of time sequence for CBC+DIFF pre-dilution measurement is that: the measurement process
of the time sequence for CBC pre-dilution measurement does not include the measurement process
of DIFF channel, and thus the aspirated blood volume is half of that in the time sequence of
CBC+DIFF pre-dilution measurement.
4.5.4 Others
The processes of time sequence for CBC+DIFF peripheral whole blood measurement, time
sequence for CBC+DIFF blank detection measurement and time sequence for CBC+DIFF whole
blood measurement are totally the same.
The processes of time sequence for CBC peripheral whole blood measurement, time sequence for
CBC blank detection measurement and time sequence for CBC whole blood measurement are
totally the same.
4.6 Functional descriptions of fluid path
Functional names of
fluid
path Functional descriptions Remar k

CBC+DIFF whole Whole blood measurement for all parameters.

blood
It has all the parameters except the 10 classified parameters
CBC whole blood of the five WBC clusters, and is the whole
blood measurement.
CBC+DIFF Capillary Blood pre-dilution measurement for all
pre-dilution parameters.
It has all the parameters except the 10 classified parameters
CBC pre-dilution of the five WBC clusters, and is the Capillary
Blood pre-dilution measurement.
CBC+DIFF Peripheral whole blood measurement for all parameters.

peripheral whole
blood
CBC peripheral It has all the parameters except the 10 classified
whole blood parameters of the five WBC clusters, and is the peripheral
measurement whole blood measurement.
CBC+DIFF blank Detect all the parameters of the analyzer, and the
detection background value under the condition without any blood
sample.
Detect the CBC parameters of the analyzer, and the
CBC blank test background value under the condition without any blood
sample.
Diluent Used for providing diluent to dilute the Capillary Blood
sample in the pre-dilution mode。

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 Fluid path All the pipelines of the analyzer are perfused with the
corresponding reagents, and the status for sample test can
perfusion be reached after completion.

Replacement of
Replace the LD Lyse in the analyzer.
LD Lyse

Replace the LH
Replace the LH Lyse in the analyzer.
Lyse

Replacement of
Replace the diluent in the analyzer.
diluent

Replacement of
Replace the cleanser in the analyzer.
cleanser

Cleaning of WBC Clean the WBC chamber using the diluent; foaming for
chamber strengthened cleaning is available in the process of
cleaning.
Cleaning of RBC Clean the RBC chamber using the diluent; foaming for
chamber strengthened cleaning is available in the process of
cleaning.
Cleaning of DIFF Clean the DIFF chamber using the diluent; foaming for
chamber strengthened cleaning is available in the process of
cleaning.
Cleaning of flow Clean the inside and the front and rear pipelines of the
flow cell.

cell

Cleaning of
Clean the inner and outer walls of the sampling probe.
sampling probe

Cleaning of flow
Including cleaning the flow cell, the DIFF chamber and the
cell channel related pipelines.

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 Cleaning of fluid
Clean all the devices and pipelines in contact with the
path blood samples in the analyzer using the diluent.

Evacuation of
Drain the flow cell.
flow cell

Evacuation of WBC Drain the WBC chamber.

chamber
Evacuation of RBC Drain the RBC chamber.
chamber
Evacuation of DIFF Drain the DIFF chamber.
chamber
Evacuation of Drain all the fluid in the analyzer, including those in

fluid path the devices and in the pipelines.

Firstly, drain all the fluid in the analyzer, then perfuse the
analyzer with purified water or deionized water, then drain
Shutdown after the fluid in the analyzer, and finally shut
packaging down the analyzer.

Drain the pressure

chamber
Eliminate all the fluid in the pressure chamber.

Back flushing of Back flush the WBC and RBC jewel holes by high
pressure
at the same time, so as to eliminate the pollutants.

jewel holes

Burning of jewel
Discharge-burn the WBC and RBC jewel holes at the same
hole time, so as to eliminate the pollutants.

Dredging of
Combination of back flushing and burning
jewel hole

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Startup for normal
shutdown
Startup cleaning process after the analyzer is shut down
through the “shutdown” function on the software

Startup for Startup cleaning process after the analyzer is shut down not
abnormal through the “shutdown” function on the software but by
shutdown directly powering off, etc.; the cleaning degree for this
process is greater than that for the startup after normal
shutdown.

Startup after The startup cleaning for shutdown after packaging equals
to adding the function of fluid path perfusion before the
packaging cleaning procedure for normal shutdown.

Shut down the analyzer software through the “shutdown”

Shutdown function on the software; before shutting down the
analyzer, the analyzer executes the immersing process
for each chamber and pipeline using the probe cleanser.
Entire machine
maintenance The chambers and pipelines in contact with blood samples
with cleanser in the entire machine shall be perfused, immersed and
cleaned, the immersion shall last for 15 minutes, and the
immersion can be canceled at any time.

After the probe fluid enters the analyzer and then diluted in
Cleaning of DIFF the DIFF chamber, the DIFF chamber, flow cell and the
channel with probe related pipelines in contact with blood samples shall be
fluid perfused, immersed and cleaned, the immersion shall last
for 5 minutes, and the immersion cannot be canceled
Cleaning of After the probe fluid enters the analyzer and then diluted in
WBC/RBC channel the WBC chamber, the WBC chamber, RBC chamber
with probe fluid and the related pipelines in contact with blood samples

shall be perfused, immersed and cleaned, the immersion

shall last for 5 minutes, and the immersion cannot be
canceled
Dredging of
After the flow cell is blocked, the square hole of the flow
flow cell cell shall be dredged by pressure

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 Cleaning of flow
cell with pure The square hole of the flow cell shall be immersed and
probe fluid cleaned with non-diluted probe fluid, the immersion shall
last for 5 minutes, and the immersion cannot be canceled.

Entire machine After the probe fluid enters the analyzer and then diluted,
maintenance the devices and pipelines in contacted with blood samples
with probe fluid shall be perfused, immersed and cleaned, the immersion
shall last for 5 minutes, and the immersion cannot be
canceled.

After the analyzer is not in used for longer time, turn off
Enter the sleep the laser device, recollect the sampling probe, and fill the
WBC/RBC chamber with diluent for immersion.
For the sleep time is less than 1 hour after sleep, when
Exit from sleep 1 exiting from sleep, maintain the fluid path mainly by
(less than 1 hour) flushing each chamber and pipeline, in order to ensure
the measurement accuracy of the analyzer.
Exit from sleep 1 For the sleep time is more than 1 hour and less than 3 hours
(greater than 1 hour, after sleep, when exiting from sleep, maintain the fluid
less than 5 hours) path mainly by flushing each chamber and pipeline, in
order to ensure the measurement accuracy of the
analyzer.
For the sleep time is more than 3 hour and less than 5 hours
Exit from sleep 3 after sleep, when exiting from sleep, maintain the fluid
(greater than or path mainly by flushing each chamber and pipeline, in
equal to 5 hours) order to ensure the measurement accuracy of the
analyzer.
Positive pressure
Inspect whether the process of creating positive pressure is
self-inspection normal

Vacuum
Inspect whether the process of creating vacuum is normal
self-inspection

Pressure release Release the pressure in the pressure chamber and the
pipelines

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 Immersion of
entire machine with
cleanser Perfuse, immerse and clean the devices and pipelines in
contact with blood samples.

Immersion and
cleaning of
entire machine Clean the devices and pipelines immersed with cleanser

Initialization of
Recover all the fluid path devices to the prepared status for
fluid path measurement

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 V.Mechanical system
5.1 Host structure
5.1.1 Main panels structure of the analyzer

BK-6310 structure (front panel)

1. Touch screen 2. Sampling probe
2. Aspiration key 4. Laser device status indicator
5. Machine status indicator

BK-6310 structure (back panel)

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 BK-6310 structure with the face shell removed

1. Sampling probe assembly 2. Hydraulic sensor

2. Valve 5 4. Swab
5. Sampling probe 6. Valve 6
7.Valve 7 8. Suction key
9. Sample syringe 10. Diluent syringe
11. Valve 1 12. Valve 2
13. Valve 3 14. Valve 4
15. Shielding case for industrial control board

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 BK-6310 structure with the right side plate removed

1. Optical path assembly 2. Valve SV25

3.Valve SV11 4.Valve SV12
5.Sampling probe assembly 6. Gas pressure chamber
7.Valve SV13 8. Valve SV14
9.Fluid suction pump 10. Valve SV23
11.Valve SV22 12. Valve SV21
13.Isolation chamber 14. Valve SV 20
15.Valve SV16 16. DIFF chamber
17.Valve SV17 18. Valve SV18
19. Valve SV19 20. WBC/RBC chamber

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 BK-6310 structure with left right-side plate removed

1.Main control board 2. Hard disk assembly

3.Position of Lyse 1 4. Position of Lyse2
5.Sensor for Lyse 1 6. Sensor for Lyse 2
7. Driver board 8. CBC pre-amplifying board
5.2 Removal and installation of host
The contents of this section mainly describe the methods and steps for replacing each assembly.
5.2.1 Opening and right-side doors
As shown in the figure, remove the screws on the back of the side panel with a screwdriver, and push
the side panel in the arrow direction as shown in the figure to take off the side panel.

85
86
5.2.2 Opening of front face shell
1.Removal
As shown in the figure, remove the four screws in the figures below to take off the face shell.

2.Installation Complete the installation following the steps opposite the above.
5.2.3 Removal and installation of top plate
1.Removal
After removing the left and right doors, as shown in the figure, remove the arrow-indicated 3 screws
with a cross screwdriver, and push the top plate to the rear of the machine to take off the top plate.

87
2.Installation
Complete the installation of top plate following the steps opposite the above.
5.2.4 Removal and installation of industrial control board (for BK-6310 model only)
Notice: Before the operation in this section, please power off the machine.
1.Removal
Firstly, remove the left side plate, the right side plate and the top cover.
As shown in the figure below, firstly remove the 2 screws for fixing 2 positions of the fluid baffle
with a cross screwdriver. Remove the fluid baffle. Then remove the 5 screws for fixing 1 position.
Remove the shielding case for industrial control board.

88
As shown in the figure below, remove the 5 screws for fixing the industrial control board with a
cross screwdriver to take off the industrial control board.

2. Installation
Complete the installation of industrial control board following the steps opposite the
above.

89
5.2.5 Removal of cooling fan, main control board and CBC pre-amplifying board
Notice: Before the operation in this section, please power off the machine.
1.Removal
The machine with the left door plate, the right side plate and upper cover plate open is shown in the
figure:
1)Remove the cooling fan, remove the 4 plastic screws for fixing the fan with a cross screwdriver,
notice that the plastic nuts matched with the screws will fall, and pay attention to the collection.
2)Remove the CBC pre-amplifying board, and remove the 4 screws for fixing the PCBA board with
across screwdriver to take off the board.
3)Remove the main control board, and remove the 4 screws for fixing the main control board with a
cross screwdriver to take off the main control board

2.Installation
Complete the installation of cooling fan, the CBC pre-amplifying board and the main control board
following the steps opposite the above.
5.2.6 Removal and installation of driver board
Notice: Before the operation in this section, please power off the machine.
1.Removal
As shown in the figure below, remove the parts 1, 2 and 3. For the methods of removal, see Section
5.2.5.

90
Remove the 4 screws for fixing the support plate for circuit board as shown by 1 in the figure below,
and take off the support plate for circuit board.

Remove the 4 copper studs for fixing the driver board as shown by 1 in the figure below, and then
take off the driver board as shown by 2 in the figure below.

91
2.Installation
Complete the installation of driver board following the steps opposite the above.
5.2.7 Removal and installation of hard disk
Notice: Before the operation in this section, please power off the machine.
1.Removal
Remove the 2 screws in the arrow-indicated positions in the figure below to pull the hard disk
assembly from the side of the machine.

2.Installation
Complete the installation of the hard disk assembly following the steps opposite the above.

92
5.2.8 Removal and installation of RFID assembly
Notice: Before the operation in this section, please power off the machine.
1.Removal
1)As shown in the figure, remove the left and right door plates, and remove the upper cover plate of
the machine (see the descriptions above).
2)Remove the face shell assembly 3. (See the descriptions above).
3)Firstly, unplug the wires connected to the RFID assembly, and then remove the 4 screws shown by
1 in the figure to take the RFID assembly 2 from the face shell.

2.Installation
Complete the installation of the RFID assembly following the steps opposite the
above.
5.2.9 Replacement of fuse
Notice: Before the operation in this section, please power off the machine, and unplug the power
cord.
As shown by 1 in the figure below, push the fuse box open with a straight screwdriver to see the fuse
in the box, and then replace the fuse.

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5.2.10 Removal and installation of power supply assembly
Notice: Before the operation in this section, please power off the machine.
1.Removal
1)Unplug the power cord of the machine.
2)Remove the left side plate, the back side plate and the top cover plate of the machine, unplug the
power plugs on the main control board, the driver board and industrial control board.
3)Sort the power output wires according to the machine structure, so that they can be taken away
together with the power pack.
4)As shown in the figure, remove the screws (totally in 6 positions) in the arrow-indicated 1 position
and 3 positions with a cross screwdriver, and pull out the power supply assembly 2 towards the back
of the machine. At the same time of pulling it way, pay attentions to sorting the power output wires
while pulling. Prevent the wires from being broken.

94
2.Installation
Complete the installation of the power supply assembly following the steps opposite the above.

5.2.11 Removal of counting chamber assembly

Notice: Before the operation in this section, please drain the reagents in the corresponding assembly,
prevent fluid from getting in contact with skin and falling onto the machine, and power off the
machine.
1.Removal
Remove the right side plate and the top cover plate of the machine, as shown in the figure:
1)Remove the WBC housing as indicated by 2 in the figure
2)Unplug the pipelines (7 pieces) connected with the chamber.
3)Remove the top plate, and unplug the (3 pieces) connecting wires between the reaction chamber
and the CBC pre-amplifying board
4)Remove the 4 screws indicated by Arrow 1 with a cross screwdriver to take off the counting
chamber assembly.

95
When removing the counting chamber, if the 4 plastic bolts’ screw thread has been wear down,
replace them by new bolts, which are included in package as standard accessories.
2.Installation
Complete the installation of the counting chamber assembly following the steps opposite the above.
Pay attention to that on completion of the counting chamber assembly, before connecting the 3 wires
to the CBC pre-amplifying board, it is necessary to test with a multimeter that the counting chamber
assembly and the frame are not conductive. If they are conductive, it is necessary to inspect whether
the insulation sleeve mentioned in the previous step is installed correctly.

5.2.12 Removal and installation of DIFF chamber assembly

Notice: Before the operation in this section, please drain the reagents in the corresponding assembly,
prevent fluid from getting in contact with skin and falling onto the machine, and power off the
machine.
1.Removal
Remove the right side plate of the machine, as shown in the figure:
1)Owing to the spatial operation, make sure to remove the housing of the counting chamber as
indicated by 1
2)Remove the 3 screws indicated by Arrow 2 with a cross screwdriver
3)Unplug the pipelines (3 pieces) connected with the DIFF chamber and the connecting wires (3
pieces).
4)Then take the DIFF out of the machine

96
2.Installation
Complete the installation of the DIFF chamber assembly following the steps opposite the above.

5.2.13 Removal and installation of sampling assembly and sampling probe

Notice: Before the operation in this section, please drain the reagents in the corresponding assembly,
prevent fluid from getting in contact with skin and falling onto the machine, and power off the
machine.
1.Removal
1)Removal of sampling assembly. Firstly, remove the left side plate, the right side plate, the upper
cover plate and the front face shell of the machine, because the pipelines will be unplugged, and
these places will be occupied. Then place the sampling probe indicated by 3 in the figure below on
the top of the assembly, in order to prevent rear collision.
a.Unplug the pipelines (3 pieces) connected with the assembly, and make sure to unplug the
pipelines from the end connected with the valve or syringe.
b. Unplug the two wires; one of the two wires is the connecting wire between the horizontal motor
and the horizontal optocoupler
c. As shown in the figure below, remove the screws in the 6 positions indicated by Arrow 1 and
Arrow 4 with a cross screwdriver.

97
2)Removal of sampling probe
a.Push the sampling probe assembly to the lower position of the assembly.
b.Cut off the 2 binding strips on the sampling probe assembly for fixing connecting pipelines of the
sampling probe.
c.Unplug the pipeline connected to the upper part of the sampling probe.
d.As indicated by 1 in the figure below, unscrew the 2 cross recessed head screws for fixing the
sampling probe assembly with a cross screwdriver.
e.Take out the sampling probe upwards

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2.Installation
Complete the installation of the sampling assembly or sampling probe following the steps opposite
the above.
Notice: When installing the sampling probe, pay attention to the following 2 points:
1)The sampling probe has poka-yoke, and make sure to choose the correct direction in installation.
Otherwise, it is unable to align with the threaded hole.
2)The pipeline (EVA tube) connected with the sampling probe is directly inserted into the upper end
of the sampling probe, then the pipeline is externally wrapped by the changeover tube, and the true
sealing is the matching of the upper end of the sampling probe with the EVA tube rather than the
changeover tube.

5.2.14 Removal and installation of waste fluid pump assembly

Notice: Before the operation in this section, power off the machine, and prevent fluid from getting in
contact with skin and falling onto the machine.
1.Removal
Remove the right side plate, as shown in the figure below, and unscrew the arrow-indicated 2 screws
at 1.

2.Installation
Complete the installation of the waste fluid pump assembly following the steps opposite the above.

99
5.2.15 Removal and installation of syringe assembly
Notice: Before the operation in this section, power off the machine, and prevent fluid from getting in
contact with skin and falling onto the machine. Power off the machine.
1.Removal
Remove the left side plate, the right side plate and the top cover, and remove the front face shell, as
shown in the figure below:
1)Remove the sample syringe 4, unscrew the arrow-indicated 4 screws at 6 and 2 with a cross
screwdriver, take off the sample syringe, and unplug the terminals on its motor and optocoupler.
2)Remove the diluent syringe 3, unscrew the arrow-indicated 4 screws at 1 and 5 with a cross
screwdriver, take off the diluent syringe, and unplug the terminals on its motor and optocoupler.

2.Installation
Complete the installation of each syringe assembly following the steps opposite the
above.

5.2.16 Removal and installation of electromagnetic valve

Notice: Before the operation in this section, power off the machine, and prevent fluid from getting in
contact with skin and falling onto the machine.
1.Removal
Open the left side plate, the right side plate and the upper cover plate, and then remove the face shell
assembly .As indicated by 1 and 2 in the figure below, unscrew the arrow-indicated 2 screws for
locking the electromagnetic valve with a cross screwdriver, take off the electromagnetic valve from
the front panel, and unplug the terminal connected to the rear after taking off the electromagnetic
valve. Pay attention to laying the wires gently, and prevent the wires from rebounding into the
mounting hole of the electromagnetic valve.

100
On the right side of the machine, unscrew the arrow-indicated 2 screws for locking the
electromagnetic valve with a cross screwdriver, take off the electromagnetic valve from the side plate,
and unplug the terminal connected to the rear after taking off the electromagnetic valve. Pay
attention to laying the wires gently, and prevent the wires from rebounding into the mounting hole of
the electromagnetic valve.

2.Installation

101
Complete the installation of each electromagnetic valve following the steps opposite the above.

5.2.17 Removal of hydraulic sensor

Notice: Before the operation in this section, power off the machine, and prevent fluid from getting in
contact with skin and falling onto the machine.
1.Remove the left side plate, the right side plate, the top cover plate and the face shell assembly, and
remove the fluid baffle fixed on the industrial control board.
2.As shown in the figure, unscrew the arrow-indicated 2 screws with a cross screwdriver.
3.Take down the hydraulic sensor indicated by 2, and unplug the connecting wire from the back to
remove the hydraulic sensor.

5.2.18 Removal of level sensor assembly

Notice: Before the operation in this section, power off the machine, and prevent fluid from getting in
contact with skin and falling onto the machine.
1.Remove the diluent level sensor.
1)Remove the right side plate.
2)As shown in the figure, unscrew the arrow-indicated 2 screws with a cross screwdriver.
3)Take down the level sensor indicated by 2, and unplug the connecting wire on the back to remove
the level sensor assembly.

102
Remove the left side plate of the machine, as shown in the figure below.
2.Remove the level sensor for Lyse 1
1)Firstly, unplug the pipelines at both ends of the level sensor for Lyse 1.
2)Remove the 2 screws indicated by 2 in the figure below,
3)Take down the level sensor for Lyse 1 indicated by 2 in the figure, and unplug the connecting wire
on the back to remove the level sensor for Lyse 1
3.Remove the level sensor for Lyse 2
1)Firstly, unplug the pipelines at both ends of the level sensor for Lyse 2.
2)Remove the screws at both ends of the sensor
3)Take down the level sensor for Lyse 2 indicated by 3 in the figure, and unplug the connecting wire
on the back to remove the level sensor for Lyse 2

103
5.2.19 Removal and installation of optical path assembly
Notice: Before the operation in this section, power off the machine, and prevent fluid from getting in
contact with skin and falling onto the machine.
1.Removal
It is noticed that when the optical path is taken off, firstly unplug the pipeline at the bottom of the
optical path. Carefully place the ducts extending from the lower part of the optical path, in order to
avoid damage the duct.

2.Installation
Complete the installation of the optical assembly following the steps opposite the above.

5.2.20 Removal and installation of board card in the optical assembly

Notice: Before the operation in this section, please power off the machine.
1. Removal
Remove the right side plate of the machine and remove the top cover, as shown in the figure below,
remove the arrow-indicated 5 screws at with 1 and 2 with a cross screwdriver, and take off the big
cover of the optical path.

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As shown in the figure:
1).Remove the SSC pre-amplifying board, remove the arrow-indicated screws (totally 4 pieces) in 1
position with a socket hexagonal head wrench, and unplug the rear wires to take out the circuit board.
2).Remove the laser driver board, remove the arrow-indicated screws (totally 4 pieces) in 2 positions
with a socket hexagonal head wrench, and unplug the rear wires to take out the circuit board.
3).Remove the FLC pre-amplifying board, remove the arrow-indicated screws (totally 4 pieces) in 3
positions with a socket hexagonal head wrench, and unplug the rear wires to take out the circuit
board.
4).Remove the FSC pre-amplifying board, remove the arrow-indicated screws (totally 4 pieces) in 4
positions with a socket hexagonal head wrench, and unplug the rear wires to take out the circuit
board.

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2..Installation
Complete the installation of each board card of the optical system following the steps opposite the
above.
3.Adjustment of laser driver board
■Power off the machine.
■Weld to remove the connection of the red connecting wire (LD_A) on laser driver board shown in
the figure with the board with a soldering iron. As shown in the figure below.

■ Connect the plug wire of the multimeter to the current position, and set the measuring range at
200mA of DC current gear. As shown in the figure below.

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■Connect the red probe of the multimeter with the red wire, and contact the black probe with the
LD_A corner of the laser driver board. As shown in the figure below.

■Power on the analyzer, rotate the adjustable resistor (blue) on the laser driver board with a straight
screwdriver, rotate clockwise to reduce the current, and rotate counterclockwise to increase the
current, until the current displayed on the multimeter is just 85mA. As shown in the figure below.

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On completion, weld the red wire back to the LD_A corner, and recover to the original shape.

5.2.21 Replace the flow cell

5.2.21.1 Remove the flow cell

1.Unplug the pipeline of the flowing chamber, and pay attention to avoiding the reagent in the
pipeline from contacting the outer surface of the flow cell.
2.Remove the 2 screws as shown in the figure, and remove the lateral lens assembly.

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3.Remove the 2 fixing screws on the flow cell seat, and lift up the flow cell in the vertical direction
to remove the flow cell.

5.2.21.2 Debugging after replacement of flow cell

1.Loosen the 2 screws on the flow cell seat, and adjust the flow cell seat to make the reflected light
deflect to the left for about 2.5mm from the small slit of front light in the horizontal direction. See
the figure below, and then fasten the 2 screws on the flow cell seat.

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2.Adjust the adjusting screws for the angle of the fixing board of the flow cell; see the figure below,
make the laser beam pass through the center of the flow cell; see the figure below, it is required to
adjust to the proper position when the luminance of the small spots in the green circle is generally the
same.

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3.Lock the adjusting nut, and pay attention to never rotating the bolt any more in the locking process.

5.2.21.3 Debugging of optical gain

See Section 6.1.3 for details.

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 VI.Parameter adjustment setting
6.1 Gain adjustment
6.1.1 Conditions for gain adjustment
■Conditions for CBC gain adjustment
1.Replace the reaction chamber.
2.Replace the jewel hole.
3. Replace the CBC board.
4. Replace the main control board.
■Conditions for DIFF gain adjustment
1.Replace the main control board.
2.Replace the FS pre-amplifying board.
3.Replace the FL pre-amplifying board.
4.Replace the SS pre-amplifying board.
6.1.2 CBC gain adjustment
Enter the interface of “ ” →“Service” → “Debug”→ “CBC Gain”.

Perform the sample analysis using the mid-value quality control in this interface for 3 times
successively, and calculate the mean values of “lymphocyte peak” and “RBC main peak” for 3 times,
respectively; the mean values of “lymphocyte peak” and “RBC main peak” are required to be within
the ranges of 67±1.5 and 89±1.5, respectively, and “HGB blank AD” is required to range from 2800
to 3000, if:
■the “lymphocyte peak” is greater, adjust to reduce the “WBC gain” value; the “lymphocyte peak” is
smaller, adjust to increase the “WBC gain” value.
■the “RBC main peak” is greater, adjust to reduce the “RBC gain” value; the “RBC main peak” is
smaller, adjust to increase the “RBC gain” value.
■the “HGB blank AD” is greater, adjust to reduce the “HGB blank AD” value; the “HGB blank AD”
is smaller, adjust to increase the “HGB blank AD” value.
6.1.3 Adjustment of DIFF gain (fresh blood )
Enter the interface of “ ” →“Service” → “Debug” →“DIFF Gain (fresh blood)”.

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Perform the sample analysis using the mid-value quality control in this interface for 3 times
successively, and calculate the mean values of “gravity center of neutrophil in FS direction”, “gravity
center of lymphocyte in FL direction” and “gravity center of neutrophil in SS direction” for 3 times,
respectively; the mean values of “gravity center of neutrophil in FS direction”, “gravity center of
lymphocyte in FL direction” and “gravity center of neutrophil in SS direction” are required to be
within the range in the table below.
Parameter Target Range of
value deviation
Gravity center of
neutrophil in FS 650 ±10
direction
Gravity center of
neutrophil in FL 550 ±10
direction
Gravity center of
neutrophil in SS 300 ±30
direction
Gravity center of
lymphocyte in FL 280 ±15
direction

If the FS gravity center is not within the range: lower:Slightly adjust to increase the FS gain;
higher:Slightly adjust to reduce the FS gain.
If the FL gravity center is not within the range: lower:Slightly adjust to increase the FL gain;
higher:Slightly adjust to reduce the FL gain.
If the SS gravity center is not within the range: lower:Slightly adjust to increase the SS gain;
higher:Slightly adjust to reduce the SS gain.

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6.2 Adjustment of mechanical position
6.2.1 Conditions for adjustment of mechanical position
1.The sampling assembly has been replaced.
2 .The position optocoupler in the horizontal or vertical direction on the sampling assembly has been
replaced.
3.The reaction chamber assembly has been replaced: DIFF chamber assembly and WBC/RBC
reaction chamber assembly.
6.2.2 Mechanical debug
Enter “ → Service →Debug → Machinery” to confirm each position of the sampling probe.

【1】 For the position circled by red square in the figure, it is only necessary to confirm whether the
value on the analyzer is the same as the value in the square.
【 2 】 In the process of position detection, it is prohibited to click the “acquire steps” button at
random, otherwise it will lead to the problem of error in pointer position.
【 3 】 Please perform “Initialization” before detection, and make sure to perform “Initialization”
after detecting each position.
【 4 】 Firstly, detect whether the horizontal position is correct; taking the detection of “DIFF
chamber” position as an example, click the “Confirm position” button under the DIFF chamber
position, and then judge whether the sampling probe assembly is moved to the DIFF chamber
position; the standard for judgment is that: click the “power off vertical motor” button, and then
manually move the sampling probe down until the sampling probe is inserted into the DIFF chamber;
the sampling probe is required to be near the middle position of the chamber in the horizontal
direction; if the deviation is greater, firstly inspect whether the horizontal belt is loose; if yes, adjust
the horizontal belt, and then perform the detection; Make sure to perform “Initialization” before the
next detection. If it is not the problem of looseness in the horizontal belt, it is necessary to adjust the
probe position according to the following methods: click “power off horizontal motor” and “power
off vertical motor”, manually move the movable needle frame horizontally to the DIFF chamber
position, then insert the sampling probe into the DIFF chamber for about 30mm, adjust the movable
needle frame to make the sampling probe in the middle position at the port of DIFF chamber in the
horizontal direction, and then press the “acquire steps” button corresponding to the “DIFF chamber”

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position in the horizontal position; on completion, the steps of the DIFF chamber in the horizontal
direction will appear in the input field of the DIFF chamber position; click the “set” button to finish
the adjustment. On completion of position adjustment, perform confirmation detection for the
debugging results, and see steps [3] and [4] for the methods.
【5】 The detection steps for other positions are the same as [4].
【 6 】 For detection in the vertical direction, the standard for judging whether it is in the correct
direction is that: the distance from the tip of the sampling probe to the bottom of each chamber is
about 2mm. if the deviation is greater, adjust the needle position in the vertical direction; taking the
position of DIFF chamber in the vertical direction as an example according to the following methods:
click “power off horizontal motor” and “power off vertical motor”, manually move the movable
needle frame horizontally to the DIFF chamber position, then insert the sampling probe into the
DIFF chamber for about 30mm, adjust the movable needle frame to make the sampling probe at
about 1mm on the right of the middle position at the port of DIFF chamber in the horizontal direction,
insert the sampling probe to the bottom of the DIFF chamber (just contact with the chamber bottom),
and then click the “acquire steps” button corresponding to the “DIFF chamber” position in the
vertical position; on completion, the steps of the DIFF chamber in the vertical direction will appear
in the input field of the DIFF chamber position, and the steps shall be rewritten in the input field after
subtracting the steps by 16; click the “set” button to finish the adjustment.
【7】 The sampling position in the vertical direction shall be confirmed according to the
following method: it is required to extend the sampling probe from the swab for 91~92mm under the
condition that the swab closely adheres to the lower edge of the mounting position, if it is not within
the range, it can be adjusted by increasing or reducing the steps in the position.

6.3 Adjustment of sensor alarm value

6.3.1 Conditions for adjustment of sensor alarm value
1.The analyzer always alarms for a certain or several sensor parameters.
2. Replace the sensor.
3.The pipeline in the sensor has been removed.
6.3.2Adjustment of sensor alarm value
Enter the interface of “ → Service →Debug→ Sensor”.

1.Take the connecting pipelines of all reagents out of the respective reagent bottle or bucket, enter
interface of reagent replacement to replace each reagent, drain the reagents in the pipelines in the
diluent sensor, the LH sensor and the LD sensor, and then input the value by adding 100 to the
readings corresponding to the “current value” in the input fields behind diluent deficient, LH
deficient and LD deficient.
2.Ensure that the value in the input filed behind the “alarm value for fluid path pressure” is 200.

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3.Click the “Set” button to save.
4.If a single sensor is involved, it is only necessary to debug the corresponding parameters.

6.4 Pressure adjustment

6.4.1 Conditions for pressure adjustment

1.Replace the driver board (Air pressure sensor)
6.4.2 Pressure adjustment
Enter the interface of “ → Service →Debug → Pressure”.
1.Connect the pressure gauge to the upmost spare pipe joint of the pressure chamber.
2.Ensure that the valve number in “Valve” is identical with the figure below.
3.Click the “pressure zeroing” button in the interface; input 1900 in the edit box corresponding to
“Create AD”, then click “Create AD”, at the moment, read the reading of the pressure gauge,
with the unit of “KPa”, input the reading into the edit box corresponding to “actually measured
pressure”, click the “Up” button, and then click the “Pressure zeroing” button in the interface;
according to the above steps, test the pressure values when "Create AD" is 2290, 2550, 2800,
2950, 3400 and 3500, click the “Calculate” button to finish the calculation, and then click the
“Download” and “Save” button to finish pressure calibration.

6.4.3 Temperature calibration of DIFF chamber

6.4.4 Conditions for temperature calibration of DIFF chamber
1.Replace the DIFF chamber.
6.4.5 Temperature calibration of DIFF chamber
Enter the interface of “ → Service →Debug →Basic Parameter”.

1. Measure the current ambient temperature A, input A+15 in the front edit box, input “0” in the edit
box behind, then click “set”, and wait for 15 minutes.
2.Insert the temperature sensor temperature gauge into the reagent in the DIFF chamber, perform

116
blank measurement in the CBC+DIFF mode, observe the measured maximum temperature value of
the temperature sensor within 5s after the sampling probe is inserted into the DIFF chamber, and
measure for 3 times successfully to calculate the mean value B of the measurement results; if
B>A+15, input: -(B-(A+15)) in the input box behind; if B<A+15, input: A+15-B in the input box
behind.
3.Modify the temperature value in the front edit box into 30, and click “Set” to finish the calibration.
6.4.6 Export and import of delivery configuration parameters
Enter the interface of “Menu” →“Service” → “Data Export”:

Click “Backup” or “Restore” in the configuration parameter box to realize the backup and recovery
of configuration parameters, respectively.

6.5 Test
6.5.1 Functional test
1.Enter “ →Service →Self-test”, as shown in the figure below:

Detection requirements:
■The devices must be detected in turn, and it is not allowed to detect the next device before finishing
the detection of the former device.
■Each device shall be in steady running without stagnation and abnormal noise.
■For the detection of valves, 23 sounds for opening and closing the valves shall be heard.
■No alarm is allowed for the detections of positive pressure and vacuum.
2.Grounding inspection
Use the 200ohm gear of the multimeter to test whether the resistance between all screws of the back
plate and the grounding terminal of the power supply is less than 0.5 ohm; it is unqualified if it is

117
greater than 0.5 ohm.
3.Inspection of software version
Inspect whether the versions of the upper computer software, middle computer software, driver board
software, fluid path time sequence and FPGA are conforming with BOM; it is unqualified if they are
not conforming.
4.Inspection of pressure detection
Enter the interface of “ ” →“Service” → “Debug”→ “Pressure”; connect the pressure gauge to
the upmost pipe joint of the pressure chamber.
Detection of positive pressure：Firstly click the “pressure zeroing” button, then input the ADvalue
(such as 2900) exceeding the “Sampling AD” value (the “Sampling AD” value is supposed to be
2800 at pressure zeroing) in the input box of “Create AD”, then click “Create AD”, and at the
moment, compare the pressure value in the input box of “Sampling pressure” with the pressure value
directly read on the pressure gauge; if the pressure deviation is within ±1Kpa, the detection of
positive pressure is qualified; then click the “Pressure zeroing” button, and release the pressure.
Detection of negative pressure: The steps for the detection of negative pressure are totally the same
as those for the detection of positive pressure, but the AD value (such as 2700) less than the
“Sampling AD” value is inputted in the input box of “Create AD”.
5.Inspection of waste fluid alarm function
In the testing process, when the floating ball on the waste fluid sensor is pushed to the upper part, the
analyzer interface will prompt for full waste fluid; it is unqualified if the prompt is not heard.
6.Inspection of prompt tone and alarm sound
In the report interface, press the suction key for normal test; when the sampling probe is moved up
on completion of sample suction, there will be voice prompt, and it is unqualified if the voice prompt
is not heard.
6.5.2 Performance test
1.Blank counting: In the report interface, in the CBC+DIFF whole blood mode, it is required to
perform blank counting for 3 times successively, and the results shall all meet:
WBC≤0.2,RBC≤0.02, PLT≤10 and HGB≦1; if it still fails to meet the requirements, it is necessary
to inspect whether the reagent is polluted, and the analyzer shall be cleaned and maintained; if it still
fails to meet the requirements, it will be processed according to the processing for faulty machine;
2.Test of carried contamination: In the report interface, in the CBC+DIFF whole blood mode, firstly
perform the test with high-value quality control for 3 times, then perform the blank test for 3 times,
and record the test results; calculate the rate of carried contamination according to theresults. The
rate of carried contamination is required to be: WBC≦0.5%, RBC≦0.5%, PLT≦1% and
HGB≦0.5%. Note: Requirements for high-value sample: WBC＞15, RBC＞6, PLT＞200 and HGB
＞ 300; low-value sample is blank test.
3.Repeatability test: In the report interface, select the CBC+DIFF whole blood mode, perform the
test using more than 1mL of normal fresh human blood for 10 times successively, and calculate the
CV value of the 10 test results. Test 3 human blood samples successively; the CV value of each
blood sample is required to meet: WBC≦ 2% RBC≦ 1.5% PLT≦ 4% HGB≦ 1.5%
MCV≦ 1%MPV≤4％ NEUT%±4 LYMP%±3 MONO%±2 EO%±1.5 BA%±0.8 .
4.Analyzer calibration: Use more than 2mL of mid-value quality control blood, perform the test for 3
times on the standard machine, calculate the mean values of WBC, RBC, HGB, PLT, MCV and
MPV as the target values for quality control, respectively; or use the fresh blood samples which have
been tested on other blood cell analyzer and printed with reports, and take the test results on the
reports as the target values.
①Calibration in whole blood mode: In the interface of fresh blood calibration, select the whole
blood mode, input the target values of the mid-value quality control blood in WBC, RBC, HGB, PLT,
MCV and MPV, and perform the test on the machine using mid-value quality control (or fresh blood

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samples) for at least 3 times successively; the CV value of the test results must meet:
WBC≦2% RBC≦1.5% PLT≦4% HGB≦1.5% MCV≦1%, and the results are saved;
if the CV value of the test results cannot meet the requirements, it is necessary to recalibrate.
② Calibration in pre-dilution mode: In the interface of fresh blood calibration, select the pre-dilution
mode, input the target values of the mid-value quality control blood (or fresh blood sample) in WBC,
RBC, HGB, PLT, MCV and MPV; press “Add diluent”, place the test tube under the sampling
needle, press the suction key for 4 times to add the diluent by the analyzer, take 80uL of blood
sample using the 100uL pipette, wipe off the outer wall of the pipette head, then add the blood
sample into the test tube which has been added with the diluent, perform the test after fully mixing,
and repeat the test for at lest 3 times; the CV value of the test results must
meet: WBC≦2% RBC≦1.5% PLT≦4% HGB≦1.5% MCV≦1%, and the
results are saved; if the CV value of the test results cannot meet the requirements, it is necessary to
recalibrate.
5.Test of analyzer comparability: Perform the test using more than 1mL of mid-value quality control
blood (or fresh blood) on the standard machine for 5 times, and calculate the mean value of each item
as the reference value.
① In the test interface, select the CBC+DIFF whole blood mode, test the sample for 3 times, and
calculate the mean value of each item; the percentage of deviation between the mean value and the
reference value for each item shall meet: WBC±3% RBC±2% HGB±2% PLT±5%
HCT±2%。
② In the test interface, select the CBC+DIFF pre-dilution mode, click “OK” and then press “Add
diluent”, place the test tube under the sampling needle, press the suction key for 4 times to add the
diluent by the analyzer, take 80uL of the sample using the 100uL pipette, wipe off the outer wall of
the pipette head, then add the sample into the test tube which has been added with the diluent,
perform the test on the machine after fully mixing, test the sample for 3 times, and calculate the
mean value of each item; the percentage of deviation between the mean value and the reference value
for each item shall meet: WBC±3% RBC±2% HGB±2% PLT±5% HCT±2%。

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 VII.Maintenance and Correct erroring

7.1 Analyzer maintenance

7.1.1 Daily maintenance

*If the user leaves the analyzer switched on for 24 hours, the analyzer interface will automatically
perform the action of " Start clean with probe cleanser?" for one time every 24 hours.
*When the number of sample tests is up to 200 (the number can be set), the analyzer interface will
automatically perform the action of " Start clean with probe cleanser?".
7.1.2 Weekly maintenance
*When the number of samples tested is up to 600 (the number can be set) or at 1 week (7 days)
interval, the analyzer will automatically perform the action of " Start probe cleanser soak?
7.1.3 Monthly maintenance
*Wipe the surface of the sampling needle (especially the tip position) with a soft, dustless tissue
every month to clean up the stubborn stains on the surface of the sampling needle.
*Clean the blood stains on surface of the swab, especially the blood stains on the lower surface.
7.1.4 Other maintenance
*When the syringe operation makes an abnormal sound, its screw rod should be added lubricating oil.
*Every six months, check the horizontal and vertical belt of the lower sampling components is loose
or not, if loose, tension it again.
*If necessary, according to the different fault conditions, perform different maintenance under the
"Routine Maintenance" interface, to achieve the purpose of correct erroring.
1.When there is slight pollution, you can choose to use all cleaning functions under the "Clean"
interface.

Cleaning function

2.When there is serious pollution, you can choose to use the various functions under the
"Maintenance" interface.

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 Maintenance function
*When the analyzer is moved or transported for long distances or unused for more than 6 days,
perform the "Pack" action under the "Service - Machine Maintenance" menu to complete the
cleaning and emptying of the analyzer fluid circuit and place it at a clean place.
7.2 Fault handling
7.2.1 Clinical alarm
7.2.1.1 Alarm sign
7.2.1.1.1Parameter fails to have results
If the parameter fails to have results after the test is completed, but displays a specific sign:
Table 1 Parameter Value Replaced Sign
Sig Description
n
--- Parameter is invalid or cannot be
calculated
++ Parameter is beyond the display range
+

7.2.1.1.2 Parameter is accompanied with alarm sign

Parameter value alarm sign appears with the parameter result, whose specific meaning is as shown in
following table.
Table 2 Parameter Value Alarm Sign

Sign Description
+ Parameter is beyond the measurement range
Parameter suspicious sign represents that the reliability of the
* parameter
result is not high. Specific to all parameters
Parameter Possible reasons

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 Poor hemolysis
1.
2. Erythrocytes, giant platelet and platelet
WBC aggregation interference
3. Electrical noise, bubble interference
4. Hole blockage

DIFF 1. Poor hemolysis of DIFF channel

Parameters: NEU% 2. Low value of leukocyte
LYM% MON% 3. Erythrocytes, giant platelet / platelet
EOS% BAS% aggregation interference
4. Over-hemolysis of leukocyte
5. Electrical noise, bubble interference
6. Dirty or blocked flow cell

Analyzer is uncalibrated
1.
HGB 2. High leukocyte interference and lipemia
sample
3. Abnormal HGB voltage
1. Giant platelet / platelet aggregation
interference
RBC 2. Microcyte is interfered by platelet
3. Abnormal distribution of erythrocyte
4. Electrical noise, bubble interference
5. Hole blockage
1. Giant platelet / platelet aggregation
2. Microcyte
PLT 3. Abnormal distribution of platelet
4. Electrical noise, bubble interference
5. Hole blockage

::: Hole blockage

7.2.1.1.3 Alarm prompt message

Alarm prompt message is the explain information of the alarm result, whose specific content is
shown as the following table:
Table 3 Alarm Prompt Message

Type Alarm prompt Possible reasons

message
Abnormal leukocyte 1. Abnormal sample
scatter diagram 2. Dirty or blocked flow cell
WBC Flag Abnormal leukocyte 1. Poor hemolysis
column diagram 2. Signal noise, bubble interference
3. Hole blockage
Leukocytosis WBC > set value
Leukopenia WBC < set value

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 Neutrophilia NEU#> set value
Neutropenia NEU#< set value
Lymphocytosis LYM# > set value
Lymphopenia LYM#< set value
Mononucleosis MON# > set value
Eosinophilia EOS#> set value
Basophilia BAS> set value
In DIFF scatter diagram, the particle
Primitive cell? number within the primitive cell area is
high.
Immature granulocyte? In DIFF scatter diagram, the particle
number within the granulocyte area is
high.
In DIFF scatter diagram, the particle
Left moving nucleus? number within the left moving nucleus
area is high.
Abnormal / abnormal In DIFF scatter diagram, the particle
shape lymphocyte? number within the abnormal / abnormal
shape lymphocyte area is high.
Hemolysis resistance? In DIFF scatter diagram, the number of
undissolved erythrocyte is high.
1. Giant platelet / platelet aggregation
Abnormal erythrocyte interference.
distribution 2. Microcyte is interfered by platelet.
3. Electrical noise, bubble interference.
4. Hole blockage.

Erythrocyte bimodality Abnormal sample, e.g., the patient is

under iron missing treatment, blood
transfusion, etc.
RBC Flag Polycythemia RBC > set value
Anemia HGB < set value
Uneven sizes of RDW-SD/RDW-CV > set value
erythrocytes
Large cell erythrocyte MCV > set value
Small cell erythrocyte MCV < set value
Hypochromic MCHC < set value
erythrocyte
Erythrocyte Abnormal RBC channel parameters
aggregation
HGB interference? Abnormal RBC channel parameters
Iron missing Abnormal RBC channel parameters

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 Abnormal distribution 1. Giant platelet / platelet aggregation
PLT Flag of platelet 2. Microcyte
3. Signal noise, bubble interference

4. Hole blockage
Thrombocythemia PLT > set value
Thrombocytopenia PLT ＜ set value
Abnormal PLT column diagram. If the
Platelet aggregation? distribution is wide, the tail of column
diagram is high.

7.2.2 Fault Alarm

7.2.2.1 Fault Alarm Information Overview
Fault Fault name Fault Fault name
code code
258 Communication error! 1770 Ambient temperature is abnormal!
1502 The model of analyzer is 1771 DIFF cell temperature is abnormal!
abnormal!
1503 The count times of 1902 Manipulator horizontal Steps error!
analyzer is abnormal!
1663 Time sequence is not 1903 Manipulator horizontal Home error!
founded!
1733 Adjusting pressure 1904 Manipulator horizontal is not initialized!
chamber pressure failed!
1741 No Diluent! 1905 Manipulator vertical is not homed!
1742 No LD Lyse! 1906 Manipulator vertical Steps error!
1743 No LH Lyse! 1907 Manipulator vertical Home error!
1745 Waste Full! 1908 Manipulator vertical is not initialized!
1746 Hydraulic pressure 1909 Sample syring Steps error!
abnormal!
1747 Flow cell clog! 1910 Sample syring Home error!
1748 Flow cell leaks 1911 Sample syring is not initialized!
1749 Fluidics leaks, Press 2003 Diluent syring Steps error!
lower!
1751 Drain pipe leaks 2004 Diluent syring Home error!
1755 Diluents is expired 2005 Diluent syring is not initialized!
1756 LD Lyse is expired 2101 Command is timeout!
1757 LH Lyse is expired 3201 Background test failed!
1760 WBC cell clog! 3202 Initialization failed!
1761 RBC cell clog! 6001 The parameter of control board differ from
the parameter file !
1762 Flow cell clog! 6002 The parameter of control board differ from
the parameter file !

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 1765 The laser is working
abnormally!
7.2.2.2 Fault alarm information handling
Fault Fault name Fault cause Fault handling procedure
code
Communication 1. Click the 'Correct Error' button to remove
command conflict, this error.
258 Communicati Command timeout, 2. Perform the shutdown procedure to shut
on error! unidentifiable down the analyzer and then restart it.
command 3. If the error still exists, please check or
change the
relevant board card.
The analyzer model,
analyzer serial
The model of number and other 1、 Please use the original board card and
1502 analyzer is analyzer information original software.
abnormal! saved by upper
software, middle
software, lower
software are
inconsistent
Special use for
The count closed reagent
1503 times of system: the times of 1. Please use the original board card and
analyzer is the reagents use is original software
abnormal! different in upper
software and lower
software
Time Time series software
1663 sequence is is not installed in the 1. Please use the original board card.
not founded! analyzer
1. Click "Correct error" button to perform
After finishing component initialization.
Adjusting build-up of pressure 2. If the fault still exists, please check whether
pressure in pressure chamber, the pipeline connected with the pressure
1733 chamber the pressure does not chamber has separation problem.
pressure meet the requirement 3. Check whether the liquid pump has
failed! or exceed the abnormality.
required pressure 4. Check the air pressure detection relevant
parts of the driver board.
5. At positive pressure, check SV18 and
SV23
valve; at negative pressure, check SV19 valve.

125
 1.
Click "Correct error" button to perform
During pressure component initialization.
The pressure detection, the built- 2. If the fault still exists, check whether the
1734 adjustment in up of pressure inside liquid pump has abnormality.
the pressure the pressure chamber 3. Check the air pressure detection relevant
chamber fails exceeds the required parts of the driver board.
highest limit. At positive pressure, check SV18 and SV23
valve;
at negative pressure, check SV19 valve.

1. Check whether the alarm information

corresponding reagent bottle has reagent. If
1741 No Diluent! there is no reagent, please change for another
bottle of new reagent. Then click "Correct
1.
Without reagent error" button to perform the action of changing
2. Liquid level reagent.
sensor failure 2. If the reagent is checked to have the much
1742 No LD Lyse! (pipeline remaining amount, please check whether part
bubble,reagent of the pipelines of the level
missing alarm value sensor of the alarm information
setting is not corresponding reagent have bubbles, if without
appropriate). bubbles,
3. Drive board enter
1743 No LH Lyse! failure "Service→EngineeringCommissioning→Senso
r" interface, and check whether the reagent
missing alarm value is set properly. See Section
6.3 for details.
3. Check the driver board level sensor
detection related parts.
1. Replace new waste bucket or empty the
1.
Waste bucket is waste.
full 2. If the waste bucket is not full, use a
1745 Waste Full! 2. Waste sensor line multimeter to measure the waste sensor (NC
or sensor is broken. type), if the circuit is measured to be closed
3. Drive board when the float is in the bottom and the circuit is
failure cut off when the float is at the top, the waste
sensor is normal; if not, check whether the
sensor line or the sensor is damaged.
3. Detect the relevant part of the drive plate.
1. Flow cell 1. Click "Eliminate Fault" button to perform
blockage the action of removing the blockage in the flow
2. Solenoid cell.
Hydraulic valve failure 2. Check SV11, SV13 valve.
1746 pressure 3. Hydraulic sensor 3. If the fault still exists, pull out SV11 valve 1
abnormal! or drive board pipeline, inject distilled water to the pipeline
failure. with a one-time syringe manually, and dredge
4. Hydraulic the flow cell.
sensor breakage 4. If the fault still exists, replace with new
5. Drive hydraulic sensor or check the relevant parts of
board the drive plate.

126
 breakage

1. Flow cell 1. Click "Eliminate Fault" button to perform

blockage the action of removing the blockage in the flow
2. Solenoid cell.
Flow cell valve failure 2. Check SV11, SV13 valve.
1747 clog! 3. Hydraulic sensor 3. If the fault still exists, pull out SV11 valve 1
or drive board pipeline, inject distilled water to the pipeline
failure. with a one-time syringe manually, and dredge
4. Hydraulic the flow cell.
sensor breakage 4. If the fault still exists, replace with new
5. Drive hydraulic sensor or check the relevant parts of
board the drive plate.
breakage
Flow cell 1. Pipeline fall-off 1. Check whether the solenoid valve SV1, SV3,
1748 leaks 2. Solenoid SV4, SV5, SV6, SV13 interface related
valve pipelines are fall off.

failure 2. If the pipeline connection is normal, please

3. Hydraulic sensor check whether the solenoid valve SV1, SV3,
or drive board SV4, SV5, SV6, SV13, SV25 are normal.
failure. 3. If the fault still exists, replace with new
hydraulic
sensor or check the relevant parts of the drive
plate.
1. Check whether the solenoid valve SV12,
1. Pipeline fall-off SV14, SV19, SV22, SV23 interface related
2. Solenoid pipelines are fall off.
1749 Fluidics valve failure 2. If the pipeline connection is normal, please
leaks! 3. Pressure sensor or check whether the solenoid valve SV12, SV14,
driver board failure. SV19, SV22, SV23 are normal.
3. If the fault still exists, replace with new
hydraulic
sensor or check the relevant parts of the drive
plate.
1. Check whether the solenoid valve SV16,
1. Pipeline fall-off SV17, SV18, SV20, SV23 interface related
2. Solenoid pipelines are fall off.
1751 Drain pipe valve failure 2. If the pipeline connection is normal, please
leaks 3. Pressure sensor or check whether the solenoid valve SV16, SV17,
driver board failure. SV18, SV20, SV23 are normal.
3. If the fault still exists, replace with new
hydraulic
sensor or check the relevant parts of the drive
plate.
Diluents is 1 、 Check whether the reagent has expired.
1755 expired 1 、 The reagent is If expired, please replace it, click the "Remove"

127
1756 LD Lyse is out of period of button to perform the replacement reagent.
expired validity. 2 、 If the reagent does not expire, enter
2 、 There is an "Routine Maintenance - Replace Reagent",
LH Lyse is
1757 expired error in the period of choose to replace the appropriate reagent, and
validity inputted. click "Set" to set the correct expiration date of
the
reagent.
1, click "Eliminate fault" button to perform the
WBC cell action of removing blockage.
1760 clog! 2. Perform "recoil" blockage, observe whether
there is liquid shot to the inner wall on the
opposite side of the counting cup front chamber
through the chamber jewel hole during the
1. Jewel hole recoil process, if not, continue to perform
blockage "Block Removal" action until the jewel hole is
2. Jewel hole got through.
RBC cell has bubble. 3. If perform the block removal command and
1761 clog! the jewel hole cannot be got through, please
unplug the connector of WBC chamber and
T23 tube or RBC chamber and T24 tube, use a
syringe to push and pull the rear chamber
pipeline manually, and get through the jewel
hole.
4. If the fault still exists, then replace the jewel
hole.

1. The flow cell has 1、 Click the "Eliminate Fault" button to

hole blockage. perform the flow cell block action.
1762 Flow cell 2. The flow cell has 2. Check SV11, SV13 valve.
clog! bubbles. 3. If the fault still exists, pull out SV11 valve 1
pipeline, inject distilled water to the pipeline
with a one-time syringe manually, and dredge
the flow cell.
1. Click the "Eliminate Fault" button to
1. The laser current eliminate the fault.
The laser is is abnormal. 2. Restart the machine.
1765 working 2. The laser is not 3. Check whether the laser drive board is
abnormally! powered. connected properly.
3. The laser 4 Check whether the microswitch on the laser
is damaged. drive board is pressed by the optical cover.
5 Check whether the laser is damaged.

128
 1. The ambient 1. Check the analyzer using environment
temperature is not in temperature is within the [15-30] ℃ range, if
the scope of use of beyond the normal range, the test results may
Ambient the analyzer. not be accurate.
1770 temperature 2. The ambient 2. If the ambient temperature is within the
is abnormal! temperature sensor is normal range, check whether the ambient
damaged. temperature sensor is normal.
3. Drive board 3. Check the relevant part of the drive board.
failure

1. Click the "Eliminate Fault" button to

perform the rinse cooling "DIFF Tank", if the
1. DIFF chamber temperature is still abnormal, turn off the
temperature setting analyzer power, when DIFF chamber
is abnormal. temperature is reduced, and then restart the
DIFF cell 2. DIFF chamber analyzer.
1771 temperature temperature sensor 3. Check the DIFF chamber heating film:
is abnormal! or temperature unplug below the DIFF chamber the heating
protection switch has line of coupling contact, use a multimeter to
failure. 3. Drive measure whether the heating film is normal,
board failure and the judgment standard is: if the resistance
is infinite, DIFF chamber heater is damaged,
and if conducted, normal.
4. Check the relevant part of the drive board.

1. The detection 1. Click "Correct error" button to perform

optocoupler in the component initialization.
Manipulator horizontal direction 2. Check whether the sampling component
1902 horizontal has failure. level optocoupler detection part is normal.
Steps error! 2. The sampling 3. Check whether the sampling component
component in the level motor drive and mechanical transmission
horizontal part is normal.
direction 4. Check whether the relevant part of the drive
transmission
ha
s

failure. board is normal.

3. Drive board
failure
1. During the reset 1. Click "Correct error" button to
process, through the perform component initialization.
Manipulator maximum number of 2. Check whether the sampling component
1903 horizontal steps, the level optocoupler detection part is normal.
Home error! optocoupler is still 3. Check whether the sampling component
cannot sense the level
catch. motor drive and mechanical transmission part
is normal.

129
 1. In the absence of
Manipulator initial action, let the
1904 horizontal sample needle 1. Click "Correct error" button to
i directly conduct the perform component initialization.
s not action in the
initialized! horizont
al direction.
1. Before the
Manipulator sampling needle 1. Click "Correct error" button to
1905 vertical is not running in the perform component initialization.
homed! horizontal direction,
there is no reset to
the vertical
direction.
1. The detection 1. Click "Correct error" button to perform
optocoupler in the component initialization.
vertical direction has 2. Check whether the sampling component
Manipulator failure. detection optocoupler in the vertical direction is
vertical Steps 2. The sampling normal.
1906 error! component in the 3. Check whether the motor drive of the
vertical sampling component in the vertical direction
direction and mechanical transmission part is normal.
transmission 4. Check whether the relevant part of the drive
has failure. board is normal.
3. Drive board
failure
1. During the reset 1. Click "Correct error" button to
process, through the perform component initialization.
Manipulator maximum number of 2. Check whether the sampling component
1907 vertical steps, the vertical detection optocoupler is normal.
Home error! optocoupler is still 3. Check whether the sampling component
cannot sense the vertical motor drive and mechanical
catch. transmission part is
normal.
1. In the absence of
Manipulator initial action, let the 1. Click "Correct error" button to
1908 vertical is not sample needle perform component initialization.
initialized! directly conduct the
action in the vertical
direction.

130
 1.Click "Correct error" button to perform
1. Syringe component initialization.
detection 2. Check the syringe position detection
Sample optocoupler optocoupler is normal.
1909 syringe Steps detection failure. 3. Check whether the motor drive of the
error! 2. Syringe sampling component in the vertical direction
transmission failure. and mechanical transmission part is normal.
3. Drive board 4. Check whether the relevant part of the drive
failure board is normal.

1.
Click "Correct error" button to perform
component initialization.
When the syringe is 2. Check the syringe position detection
Sample reset, the reset optocoupler is normal.
1910 syringe process is not 3. Check whether the motor drive of the
Home error! completed within the sampling component in the vertical direction
specified number of and mechanical transmission part is normal.
steps 4. Check whether the relevant part of the drive
board is normal.

Sample The syringe does not

1911 syringe is not perform an 1. Click "Correct error" button to
initialized! initialized action perform component initialization.
before the
movement.
1. Click "Correct error" button to perform
1. Syringe component initialization.
detection 2. Check the syringe position detection
Diluent optocoupler optocoupler is normal.
2003 syringe Steps detection failure. 3. Check whether the motor drive of the
error! 2. Syringe sampling component in the vertical direction
transmission failure. and mechanical transmission part is normal.
3. Drive board 4. Check whether the relevant part of the drive
failure board is normal.

1.
Click "Correct error" button to perform
component initialization.
When the syringe is 2. Check the syringe position detection
Diluent reset, the reset optocoupler is normal.
2004 syringe process is not 3. Check whether the motor drive of the
Home error! completed within the sampling component in the vertical direction
specified number of and mechanical transmission part is normal.
steps. 4. Check whether the relevant part of the drive
board is normal.

131
 Diluent The syringe does not
2005 syringe is not perform an 1. Click "Correct error" button to
initialized! initialized action perform component initialization.
before the
movement.

1. Click "Eliminate Fault" button, and conduct

1. The results of blank test again.
blank test is beyond 2. Check whether the reagent is expired or
the provisions of the contaminated.
requirements. 3. Enter
2. Counting tank is "Service→RoutineMaintenance→Clean"
contaminated or interface, perform "Liquid Circuit Clean"
Background liquid circuit action, and re-test blank after the operation.
3201 test failed! System has bubbles. 4.Enter
3. Dilution is "Service→RoutineMaintenance→Maintenance
contaminated or " interface, perform "Remove Blockage"
expired. action, and confirm the jewel hole is not
4. Jewel hole blocked and re-test the blank.
blockage 4. Use the corresponding probe liquid cleaning
5. Noise function to clean the corresponding parts.
interference. 5. Ensure that the analyzer is away from
interference sources.

Initialization
3201 failed!

1 、 Click "Eliminate Fault" button, select the

appropriate treatment according to different
The circumstances:
parameter of The parameters of 1） When the replacement of the main control
control board the main control board causes the failure, choose to download
6001 differs from board and the upper parameters from the upper software software to
the software software the main control board.
parameter are inconsistent. 2） When upper software software re-
file! installation or change causes failure, choose to
download parameters from the main control
board to the upper software software
2 、 Click "Eliminate Fault" button, select the

132
 appropriate treatment according to different
circumstances:
The 3） When the replacement of the driver board
parameter of The parameters of causes the failure, choose to download
control board the driver board and parameters from the upper software software to
6002 differs from the upper software the driver board.
the software are 4） When upper software software re-
parameter inconsistent. installation or change causes failure, choose to
file! download parameters from the driver board to
the
upper software software

133
7.2.2.3 Other faults
Order Liquid
Number circuit Possible reasons Solutions
failure
1. Check the pipeline from the swab to the liquid
pump through SV18 is connected normally and
1. Connecting whether there is leakage.
pipe is 2. Enter "Service→Self-test" interface, and check
Liquid not whether SV18 valve can work normally.
1 falls connected. 3. Enter "Service→Self-test" interface, and check the
Out of the 2. SV18 pump; if the pump cannot work, replace the pump. If
swab valve the pump can operate, open the pump head, check
failure. whether there are particles or debris in the pump
3. Pump failure. head diaphragm and other positions; if so, clear them
4. Swab wear. and re-test.
4. If the fault still exists, replace the swab.

1. Enter "Service→Self-test" interface, and check

whether SV18, SV20, SV22, SV23 valve, especially
SV20 and SV18 valve are working properly.
1. SV18, 2. Enter "Service→Self-test" interface, and check
DIFF SV20, the pump; if the pump cannot work, replace the
2 chamber SV22, SV23 pump. If the pump can operate, open the pump head,
liquid valve failure. check whether there are particles or debris in the
overflow 2. Pump failure. pump head diaphragm and other positions; if so,
3. Drain pipeline clear them and re-test.
is detached. 3. Check whether the pipeline connected to the
liquid pump, SV18, SV20, SV16, S17, SV22, SV23,
SV19 valve is
detached.
1. Enter "Service→Self-test" interface, and check
whether SV18, SV16, SV22, SV23 valve, especially
SV16 and SV18 valve are working properly.
1. SV18, 2. Enter "Service→Self-test" interface, and check
WBC SV16, the pump; if the pump cannot work, replace the
3 chamber SV22, SV23 pump. If the pump can operate, open the pump head,
liquid valve failure. check whether there are particles or debris in the
overflow 2. Pump failure. pump head diaphragm and other positions; if so,
3. Drain pipeline clear them and re-test.
is detached. 3. Check whether the pipeline connected to the
liquid
pump, SV18, SV20, SV16, S17, SV22, SV23, SV19
valve is
detached.

134
 1.SV18, 1. Enter "Service→Self-test" interface, and check
RBC SV17, whether SV18, SV17, SV22, SV23 valve, especially
4 chamber SV22, SV23 SV17 and SV18 valve are working properly.
liquid valve failure. 2. Enter "Service→Self-test" interface, and check
overflow 2. Pump failure. the

3. Drain pipeline pump; if the pump cannot work, replace the pump. If
is detached. the pump can operate, open the pump head, check
whether there are particles or debris in the pump
head diaphragm and other positions; if so, clear them
and re-test.
3. Check whether the pipeline connected to the liquid
pump, SV18, SV20, SV16, S17, SV22, SV23, SV19
valve is
detached.
1. Enter "Service→Self-test" interface, and check the
pump; if the pump cannot work, replace the pump. If
the pump can operate, open the pump head, check
whether there are particles or debris in the pump
head diaphragm and other positions; if so, clear them
and re-test.
2. Enter "Service→Self-test" interface, check valve
SV22 and three chambers corresponding discharge
1. Pump failure. valve SV20, SV16, SV17 are working properly.
There is no 2. Valve failure. 3. Enter "Service→Self-test" interface, click
bubble 3. Pipeline fall-off "Positive Pressure Test", if there is alarm prompt,
5 coming out 4.Pipeline check whether the pipeline connected with the valve
when blockage. SV20, SV16, SV17, SV22, SV12, SV14, SV19 is
blending 5.Pressure sensor detached.
well. failure. 4. Check whether the bubble pipeline (pipelines, all
isolation chamber and the corresponding valves
SV20, SV16, SV17 between all reaction chambers
and corresponding isolation chambers) is filled with
liquid.
5. Check whether the bubble pressure is within the
range of "3-7Kpa", if out of range,
enter
"Service→EngineeringCommissioning→Pressure"
interface, and recalibrate the pressure.
6. Replace the drive board, and recalibrate the
pressure.

135
 1.
Valve
failure (DIFF
chamber sample
does not 1. Enter the "Service→Self-test" interface, and
enter the sample check whether the diluent syringe and solenoid
Single DIFF preparation tube). valves SV1, SV2, SV3, SV4, SV5 are working
6 channel 2. Dilution properly.
without data syringe failure. 2. Enter the "Service→Self-test" interface, and
3. Main control check whether the diluent syringe is working
board properly.
or optical 3. Check the main control board and the optical
receiver receiving plate related parts.
board failure.

1. Sampl
e 1. Sample preparation is not in place: enter
preparation "Service→Self-test" interface, check whether the
i dilution syringe is normal and whether there is
s not in place. leakage; check whether valve SV1, SV3, SV4,
DIFF channel 2. No SV11 are normal.
The number of auxiliary 2. Check the SV4 valve and whether the thin
7 particles is sample pushing TEFLON tube flow connected by the three links
small. process or below the chamber sample needle, and whether
auxiliary sample there are creases.
pushing process is 3. Enter "Service→Self-test" interface, check
not fully realized. whether the sample syringe is normal and whether
3. Sample there is leakage.
syringe
failure.

1.
Samples are
not well mixed.
2. Sample
selection is not
good (abnormal
blood). 1. Shake the sample well.
Most 3. Counting tank 2. Select the appropriate sample.
indicators Poor is contaminated or 3. Enter "Service→RoutineMaintenance→Clean"
8 reproducibility liquid circuit interface, and perform "Clean Liquid Circuit"
system has action, and if still not work, use the probe liquid to
bubbles. clean the machine.
4. Reagent is 4. Replace reagent in expiration date or without
contaminated or pollution.
expired. 5. Eliminate the interference source.
5.Noise
interference.

136
 1. Sample 1. Select the appropriate sample.
selection is bad 2. Enter "Service→Daily
(WBC abnormal Maintenance→Maintenance" interface and perform
blood). "Remove blockage" action.
2. Jewel hole 3. Enter "Service→Daily Maintenance→Clean"
Blockage in WBC interface and perform "Clean WBC Tank" action; if
Poor WBC chamber the vacuole overflows the cup mouth after blending,
9 repeatability 3. WBC clean the inner wall of WBC chamber with soft,
chamber pollution dustless cloth manually.
4. RBC chamber 4. Observe whether the bubble blending process of
without blending the WBC chamber is normal. If no bubble comes
bubbles out, refer to the correct erroring method above "No
5. LH Lyse fault Bubble Comes Out in the Bubble Blending
Process".
5. Check whether the LH Lyse is expired, and
whether the
action to add Lyse is correct.

6. WBC gain 6.Enter"Service→Engineering

setting is Commissioning→Engineering Commissioning→CBC
inappropriate gain" interface and readjust WBC gain.
7.Noise 7. Eliminate the interference source.
interference.

1. Sample
selection is not
good (RBC/PLT 1. Select the appropriate sample.
abnormal 2. Enter "Service→Daily
blood). Maintenance→Maintenance" interface and perform
2. Jewel hole "Remove blockage" action.
blockage 3. Enter "Service→Daily Maintenance→Clean"
RBC/PLT 3. RBC interface and perform "Clean RBC chamber" action.
10 Poor chamber 4. Observe whether the bubble blending process of the
reproducibility pollution RBC chamber is normal. If no bubble comes out, refer
5. RBC to the correct erroring method above "No Bubble
chamber Comes Out in the Bubble Blending Process".
without bubbles 5.Enter"Service→Engineering
4. RBC gain Commissioning→Engineering Commissioning→CBC
setting is not gain" interface and readjust WBC gain.
appropriate. 6.Eliminate the interference source.
6.Noise
interference.

137
 1. During the
test process,
whether there is 1. Enter "Service→Daily Maintenance→Clean"
Single HGB small interface and perform "Clean WBC Tank" action; if the
repeatability is bubbles attached vacuole overflows the cup mouth after blending, clean
11 bad. on the inner wall of WBC chamber with soft, dustless
The WBC cloth manually.
chamber wall 2. Check whether the luminous diode is normal.
2. Luminous
diode
is broken.
1.
WBC
blending
failure. 1. Observe that during the measurement process,
Erythrocytes whether bubble appears in two times of beating
Normal sample have not been bubbles in WBC chamber. If no bubble appears, refer
WBC test dissolved to the according to the above correct erroring "No
12 value >20 completely. Bubble Comes Out in the Bubble Blending Process".
2. LH Lyse 2. Check whether the pipeline has bubbles when
addition is not adding LH Lyse and SV15 valve is working properly.
enough. (5H
Lyse
Running out,
SV12 valve
failure)

138
BIOBASE GROUP
2# building, No.9 Gangxing Road, High-tech Zone, Jinan City, Shandong Province,
China
Tel: +86-531-81219803/01
Fax: +86-531-81219804
Inquiry: export@biobase.com
Complaints: customer_support@biobase.cc
After-sales service: service_sd@biobase.cc; service_ivd@biobase.cc
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