Republic of the Philippines

Department of Education
REGION VII - CENTRAL VISAYAS
DIVISION OF LAPU-LAPU CITY
MACTAN NATIONAL HIGH SCHOOL

LESSON PLAN IN SCIENCE 7

Section and Time: Date: May 20,
2022

Most Essential Focus specimens using the compound microscope. Level VII
Learning
Competency (STLT-llb-2) Quarter 2nd
(MELC)

Learning Knowledge: Compare the image of an object seen by the unaided eye to Week 2
Objectives No.
the image seen under the microscope.
Day
Skills: Focus the specimen under the microscope properly.

Attitudes: Employ appropriate techniques using the compound

microscope to gather data about very small objects.

Values: Be responsible enough in using the microscope (Makatao).

Topic Focus specimens using the compound microscope Duration: 1 hour

Resources Microscope, glass slide, cover slip, water, dropper, blade, printed paper, iodine solution,
Needed Learning notes/Module in Science 7 (Week 2), audiovisual devices, cartolina, marker,
crayon

PROCEDURE:
Element of the Suggested Activities
Plan Teacher’s Activities Student’s Activities

Engage ● Daily Routine (Prayer & Attendance check) Students assigned will take charge of
the daily routine.
● Recap
- The teacher will be asking somebody to give the
recap of yesterday’s lesson. These are the following
questions:
1. What was the lesson discussed yesterday?
- It’s all about the microscope

2. What are the three main parts of the microscope?

- (1) Magnifying, (2) Illuminating and
Perfect! (3) Mechanical Parts

3. Can somebody come here in front to identify the - The learner will identify and name
parts of the microscope? the parts of the compound microscope
in front.
Bravo!

- Always carry the microscope with

4. When you try to travel the microscope from one
one hand holding the arm and your
place to another, how do you carry the microscope?
other hand supporting under the base.
Excellent!
 - It allows us to study tiny organisms
or object/Answers may vary
5. What do you think is the most significant use of the
microscope?
Awesome!

● The teacher will connect the new lesson by asking

the following questions:
-By making a wet or dry mount/
- We know for the fact that a microscope is used to answers may vary
magnify images of any tiny objects or living
organisms which are too small to be seen by the
naked eye, how do scientists or biologists prepare
the object to be studied under the microscope?

- How do we call this object/organism under

microscope study?
-Specimen

Note: The teacher will be giving reinforcement such as

chips as a point system to those who actively participate in
the class.
Note: Learning Modules/notes and video recording will be
given to the students who were not able to attend due to
illness and other reason.

Explore ● The teacher will present the topic on the screen

“Focus specimens using the compound microscope
A. Making wet mount
B. Observing specimen under the microscope”

Note: The teacher uses a powerpoint presentation in

delivering her lesson

- These questions will be asked: -A specimen is a part or sample of any

material e.g. plant, animal, paper or
1. What is a specimen?
mineral, for study or examination
under the microscope.

-There are considerations in preparing

a specimen: a. It must be small and
thin enough for light to pass through
2. How do we prepare the specimen? What are the them.
steps in making a wet mount?
b. Proper staining must be done using
iodine solution or methylene blue.
c. Proper laboratory materials should
be used and available.
 ● The teacher will present a short video on how to
prepare the wet mount using the small letter “e".
Afterwards the class will be grouped into 3 to
practice making wet mounts. The materials like a
printed paper, scissors, dropper, glass slide, cover
slip and compound microscope will be given. The
student will be given learning notes also as their
guide.

- Video link: https://www.youtube.com/watch?

v=Tl2tzBM0_ok

Part I: Making Wet Mount

1. Cut out a small letter “e” from a printed paper. Using
forceps or tweezers place it in at the center of a glass slide
in an upright position.
2. Add a drop of tap water over the specimen. It will act as a
mounting medium and make clear the image of the
specimen. Position the cover slip at 45° with one side
touching one edge of the water on the slide.
3. Slowly lower the other edge of the cover slip until it rests
on the water and the printed letter. Bubbles are perfect
circles you see on your preparation. Remove or minimize
trapped bubbles by gently tapping the cover slip with the
eraser-end of a pencil. Make the bubble move towards the
edge of the cover slip.

Part 2: Observing Specimen

1. Put the slide on the stage. Make sure that the letter “e” is
at the center of the hole in the stage and under the LPO.
Hold it firmly with the stage clips.
2. Watching from the side, carefully lower the body tube
until the end of the LPO almost touches the cover slip.
3. Look through the eyepiece. Slowly turn the coarse
adjustment upwards to raise the objective until the letter “e”
appears. Continue until you see the letter clearly. This
would indicate that you have focused it already.
Note: Learning Modules/notes and video recording will be
given to the students who were not able to attend due to
illness and other reason.

Explain QUESTIONS:
1. How do you describe the position of the letter “e” -It is inverted
as seen under the microscope?

2. How will you compare the image of the letter “e”

-The letter is magnified as seen under
that you’ve seen using your unaided eye with what
the microscope/Answers may vary
you’ve seen through the microscope?

3. When you look through the microscope and slowly

 move the slide to the right, then to the left. To -To the opposite direction
which direction does the image move?

4. Why do you have to watch from the side when

changing objectives?
-To make sure that the objectives will
not touch the glass slide or the
specimen under study causing it to
break or damage the lenses of the
objectives/answers may vary.

-To fine tune the image under study or

5. Why should the fine adjustment knob be used only make it clearer for observation.
with the HPO?

-The HPO allows an individual to

6. What are the advantages of using the HPO? the observe the specimen clearer however
disadvantages? the disadvantage is you cannot see the
whole image of the specimen because
it is already magnified 100x/Answers
may vary

The magnification of a specimen can be calculated by

multiplying the number found in the eyepiece with the
number found on the objective being used. So, if a
specimen is viewed using a 10x objective and a 10x
eyepiece it will be magnified 100 times.
-The eyepiece has a magnification of
7. Examine the numbers indicated on the eyepiece and 10x. If the cell viewed under the LPO
scanner. How much is the letter “e” you are now has 10x magnification, then the
viewing under the scanner magnified? Under the specimen viewed is 100x. While
LPO? Under the HPO? under the HPO, the specimen is
viewed 400x because HPO has a 40x
magnification.
- The answer is 5x
8. If a cell being observed has been magnified 200x (200 x /40 x=5 x)
under the HPO, what is the magnifying power of
the eyepiece used? (Math- across curriculum)

Elaborate This part will prove the mastery over the concepts they
learned in the previous activity. The teacher will regroup
the class into 3. Each group will be tasked to complete the
activity and will present their findings differently from
another group (DI). There will be groups who will do the
SCAN IT!, ILLUSTRATE IT! and ORGANIZE IT!

1. SCAN IT! (Tier 3)

Students will be asked to focus an onion cell under
the scanner and LPO and present it to the class
using the camera of the phone casted to the TV
monitor for others to see.

2. ILLUSTRATE IT! (Tier 2)

Students will be sketching the image of an onion
cell seen with and without stain under the LPO.
(MAPEH-across curriculum)
 3. ORGANIZE IT! (Tier 1)
Students will be asked to demonstrate how to focus
an onion cell from LPO to HPO while identifying
the most significant part of the cell seen under the
microscope.

Each group will be given an onion, blade, microscope, glass

slide, iodine solution, tissue and Learning module as their
guide.
*The teacher will remind the student to be careful in using
the blade so as not to cut their skin or to avoid injury.

Part 3: How to prepare a stain specimen

1. Cut the onion into quarters.
2. Remove one fleshy scale and detach carefully the
transparent skin from the inner surface of the onion
scale using the tweezer.
3. Place the thin transparent onion skin on the glass
slide with the inner surface (non-waxy slide) facing
up. Make sure that it is not folded or wrinkled.
4. Add a droplet of distilled water on the onion skin
and cover it with the slip.
5. Observe the onion cell under the microscope
shifting from the scanner to HPO.
6. Remove the slide from the stage. Using a dropper
add one or two drops of iodine solution along the
edge of the cover slip. Remove the extra liquid
from the edge of the cover slip using a tissue paper.
7. Place the slide on the stage and observe the
specimen from the scanner to LPO and HPO.
8. Record your observation of the onion slide with and
without a stain.
9. Clean the microscope after using.
10. Prepare to present the task given.

After the presentation ask this question:

1. In what ways would the microscope contribute to
the study of the cells?

- The microscope allows us to view

the cell clearly and be able to study its
2. How important is its application in the recent
parts function, how it reproduces, and
pandemic that we are facing?
its other role in the organism’s body.
(Values -Across Curriculum)

- The microscope is very significant

Note: Learning Modules/notes and video recording will be ever since, much more in today’s
 given to the students who were not able to attend due to crisis that many medical technologies
illness and other reason. use these to create a vaccine that will
help prevent the spread of the virus
and get rid of it.

Evaluate The teacher will ask the students to use their cellphones for
the evaluation. The teacher will use the Kahoot an online
game-based evaluation for the students.
(Note: There must be an internet connection in the room)

Assignment Identify the different parts of a cell and briefly describe its
function.

Prepared by:
JENNIFER T. MAGLASANG
Teacher/Demonstrator

Checked by:
RACHEL L. AYING
Department Head, MT II
Name of the School: Mactan National High School
Address: Mactan, Lapu-Lapu City
Contact Number: 239-2628
Email Address: mactannhs.jhs@gmail.com