Presentation - Automation in Micros
Presentation - Automation in Micros
1 Automation in Microscopy
2 AxioScan.Z1
3 Celldiscoverer 7
1 Automation in Microscopy
2 AxioScan.Z1
3 Celldiscoverer 7
Resolution
Flexibility Repeatability/Reliability
Sensitivity Speed
User friendly
What is Automation?
• Repeatability/Reliability:
• Limit human errors (ie: microscope setup, calibration…).
• Focus stability.
• Stable environment (ie: Temp., CO2…).
• Speed:
• Fast acquisition.
• Time in front of the system.
• Ease to use:
• Easy software with few clicks.
• Software with workflow processes.
1 Automation in Microscopy
2 AxioScan.Z1
3 Celldiscoverer 7
• Widefield acquisitions.
• ZEISS Objectives.
AxioScan.Z1
20 x
40 x
• All slide formats could be scanned from 25x72mm slides to 100x72mm slides.
• Only the tray is handled by the scanner... not the slides. It limits the risk to break
them during the loading/unloading process.
• Slides move horizontally in the scanner. It limits the risk to take off the coverslip, if
it is not fixed (ie: glycerine medium).
• For each slide, the software saves an overview image from the slide and the label.
An automatic threshold detects tissue sections using the overview image.
• Before scanning, the software will perform an autofocus and it determines the
focus.
• Then, the scanning process will start, using the focus map defined previously. The
scanner will acquire 1cm² in 2 minutes (for brightfield @20x/0.8).
• The virtual slide, a czi image, contains all the informations from the acquisition
(scaling, exposure time…).
• The images are stored with a lossless compression to avoid harddisk overflow.
1 Automation in Microscopy
2 AxioScan.Z1
3 Celldiscoverer 7
• Widefield acquisitions.
• A magnifier changer with 3 positions (0.5x, 1x, 2x) is included in the Celldiscover 7.
• Range of 8 different magnifications : 2.5x, 5x, 10x, 20x, 25x W, 40x, 50x W, 100x W.
Carl Zeiss SAS, Julien Kissenberger 2017-01-25 19
Celldiscoverer
Phase Gradient Contrast
• New contrasting method for transmitted light acquisition: Phase Gradient Contrast
(PGC)..
• Compatible with all objectives and all supports.
• PGC does not impact fluorescence (sensitivity) like standard phase contrast.
• The Celldiscoverer 7 is not only dedicated to multiwall plates, you can acquire
images on all supports, regardless of the bottom.
Gas exchanges
Standard
workspace
Automation
workspace
A developing
cricket embryo.
During the
development from
egg stage 15 to
egg stage 21.
Scale bar 200µm.
Sample courtesy
of Seth Donoughe,
Department of
Organismic and
Evolutionary,
Harvard University
Cambridge, USA
0h 22h 45h
0h 22h 45h
Top row: A series of images showing untreated control cells. Bottom Row: A series of images showing cells treated 24 h before acquisition with a
siRNA against AuroraB Kinase.
Sample courtesy of S. Reither, Advanced Light Microscopy Facility, EMBL, Heidelberg, Germany
• Parfocality
• All objectives
• All magnification changer positions
• Parcentricity
• All objectives
• All magnification changer positions
• The autoimmersion system adjusts the proper volume between the objective and
the samples. All the fluids are removed after the experiment in a specific container.