Course Title: Skills in laboratory management

L T P/S SW/FW PSDA TOTAL CREDIT UNITS

Course Code:
1 0 2 2 3 3
Credit Units: 3
Level: UNDERGRADUATE

Course Objectives: The student will:

• Illustrate a good foundation about the basic use of Inorganic and Organic chemicals used in of microbiology and analytical laboratories.
• Inculcate the approved methods for handling chemicals and instruments following mandatory safety guidelines
• Develop an understanding of how to keep records and document data.
• Recapitulate the basics of chemistry and analytical techniques for testing various compounds
• Build on their basic knowledge of computer software and maintenance of data integrity

Prerequisites:
To study this course, a student must have had the basic knowledge of chemistry and biology

Course Contents/Syllabus Weightage (%)

Module I: Basics of bioanalytical techniques 20
Understanding the principle and use of spectrophotometer, TLC, column chromatography, GC, HPLC, centrifuge, electrophoresis
Module II: Basics of microbiological techniques 20
Streaking, spread plating, fermentation of bacteria/ other microbes, autoclaving/sterilization, concept of automated labs
Module III: preparation of Buffers and common lab solvents and media for microbiology 20
Concept of acids/ bases, concentration/ dilution of solutions, titration, various microbiological medium for various types of bacteria/microbes
Module IV: Good documentation practices and storage practices for chemicals and products 20
Drafting and following SOPs, maintaining or recording data, revision/ cancellation of documents, checking leakage/ defects in chemicals,
identifying storage areas and special storage requirements for chemicals and products, maintenance and calibration of Material handling
equipment as per SOP, statistical analysis of test data, interpreting images, graphs, diagrams for typical product specifications, job cards,
procedures, material labels and safety information as provided
Module V: Ensuring cleanliness and safety in lab and proper work environment 20
 Liquid and solid waste management, concept of clean rooms, team work and coordination with supervisor, different quality management
systems (ISO-9000, ISO-14001, OHSAS-18000) and good laboratory and manufacturing practices, risk and impact of not following defined
procedures, communicating confidential and sensitive information discretely to authorized person as per the SOP, planning the sequence for
cleaning the area to avoid re-soiling clean areas and surfaces, dealing with and reporting accidental damage, caused while carrying out the
work, escalation procedures for soils or stains that could not be removed, work in a team and other behavioural skills required to support the
small group activities (e.g. quality circle, cross functional team, suggestion scheme)

Lab/Experiments
1.) Lab analytical techniques
a) Learning to use and maintain instruments like the pH meter, Spectrophotometer, centrifuge, colony counter, autoclave
b) Use analytical techniques like TLC, GC, HPLC
2.) Basic microbiological Techniques
a) Learning to start and maintain microbial cultures on solid agar plates and liquid media
b) Basic techniques, e.g. streaking, spreading, determining colony forming units
c) Fermentation techniques in shake flask
3.) Preparation of common lab solutions and media
a) Preparation of PBS
b) Preparation of buffers, microbiological media (broth and agar)
4) Application of Software Products (Office, chemsketch, scilab, matlab, hyperchem, etc.)
a) Learning to use tools e.g. Office, chemsketch, scilab, matlab, hyperchem
b) Drafting and sending emails and keeping inventories

Course Learning Outcomes:

On completion of the course the student will be able to:
1. Recall the basic microbiology and chemistry knowledge.
2. Demonstrate the proper chemical and instrument handling technique.
3. Identify discrepancy in data and defect/ fault in chemicals/products.
4. Analyze the data of tests obtained.
5. Evaluate the cleanliness and safety compliance of work place
6. Estimate by experiments the various water parameters, amount of acid /base, contamination.

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Mapping of University Graduate Attribute with CLOs:
Bloom’s Level → Remembering Understanding Applying Analyzing Evaluating Creating
CLO1: Recall CLO2: Demonstrate CLO3: Identify CLO4: Analyze CLO5: CLO6: Estimate
the proper discrepancy in data the data of Evaluate the by experiments the
S. the basic cleanliness and various water
No. Course Learning Outcomes → microbiology chemical and and defect/ fault in tests obtained safety parameters, amount
University Graduate Attributes ↓ and chemistry instrument chemicals/products compliance of
work place
of acid base
present, metal ions
knowledge handling and hydrated salts
technique
1 Discipline Knowledge & Expertise √ √
2 Self-Directed and Active Learning √ √ √ √
3 Research and Enquiry √ √ √
Information & Communication
4 √
Technology Skills
Critical Thinking & Problem-Solving
5 √
Abilities
6 Communication Skills
Creativity, Innovation & Reflective
7 √
Thinking
8 Analytical & Decision-Making Ability
9 Leadership & Teamwork
Multicultural Understanding & Global
10
Outlook
11 Integrity and Ethics
12 Social & Emotional Skills

3
 Employability, Enterprise &
13
Entrepreneurship
14 Lifelong Learning
15 Environment & Sustainability

Pedagogy for Course Delivery:

The course pedagogy will include lectures, practical, discussion, applications, presentations, reading review papers and individual. E content will
be provided to learners with a four-quadrant approach.
List of Professional Skill Development Activities (PSDA):
i) Presentation on recent research on any one topic relevant to the contents.
ii) Group discussion on applications organic/inorganic chemistry in industry
iii) Demonstration of Lab safety skills

Assessment/ Examination Scheme:

Theory L/T (%) Lab/Practical/Studio (%) Total

33% 67% 100%

Theory Assessment (L&T):

Continuous Assessment/Internal Assessment (in %)
Components (Drop
CT HA Viva Quiz PSDA ATT EE
down)
Linkage of PSDA
with Internal
Presentation GD
Assessment
Component, if any
Weightage (%) 10 7 3 5 5 5 5 60%
Abbreviations: CT – Class Test, Q- Quiz, HA- Home Assignment, ATT-Attendance, PSDA-Professional Skill Development Activities, EE-End Term Examination
Lab/ Practical/ Studio Assessment:

4
 Continuous Assessment/Internal Assessment End Term Examination
(40%) (60%)
Components (Drop down) LR P V ATT EX V
Weightage (%) 30
10 10 15 5 30
33
Abbreviations: LR – Lab record, P- Performance, V- Viva, ATT-Attendance, Ex-Experimental

Mapping Continuous Evaluation components/PSDA with CLOs

Bloom’s Level Remembering Understanding Applying Analysing Evaluating Creating

Course Learning CLO1: Recall CLO2: CLO3: Identify CLO4: Analyze CLO5: Evaluate CLO6: Estimate by
Outcomes the basic Demonstrate the discrepancy in data the data of tests the cleanliness experiments the various
microbiology and proper chemical and defect/ fault in obtained and safety water parameters, amount of
Assessment chemistry and instrument chemicals/products compliance of acid base present, metal ions
knowledge handling technique work place and hydrated salts
type/PSDA
Class test √ √ √ √
Home Assignment √ √ √ √ √ √
Viva (Theory) √ √ √
Quiz √ √ √
PSDA presentation √ √
PSDA GD √ √ √ √ √
Lab Record √
Performance √ √
Viva (Lab) √ √ √ √ √

Text & References:

• Lee, J.D. Concise Inorganic Chemistry, Pearson Education 2010
• Huheey, J.E., Keiter, E.A., Keiter, R. L., Medhi, O.K. Inorganic Chemistry, Principles of Structure and Reactivity, Pearson Education
2006.
• Carey, F. A., Guiliano, R. M.Organic Chemistry, Eighth edition, McGraw Hill Education, 2012.

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 • Loudon, G. M. Organic Chemistry, Fourth edition, Oxford University Press, 2008.
• R.Y. Stanier, General Microbiology, 5th edition, Palgrave Macmillan, 1987
• Wilson and Walker, Principles and Techniques of Biochemistry and Molecular Biology, Cambridge Univ Press, 2010
• Clayden, J., Greeves, N. &Warren, S. Organic Chemistry, 2nd edition, Oxford University Press, 2012.
• Mendham, J. Vogel’s Quantitative Chemical Analysis, Pearson, 2009.
• Harris, D. C. Quantitative Chemical Analysis. 6th Ed., Freeman (2007) Chapters 3-5.
• Harris, D.C. Exploring Chemical Analysis, 9th Ed. New York, W.H. Freeman, 2016.
• Khopkar, S.M. Basic Concepts of Analytical Chemistry. New Age International Publisher, 2009.

Suggested online links:

• http://heecontent.upsdc.gov.in/Home.aspx
• https://nptel.ac.in/courses/104/106/104106096/
• http://heecontent.upsdc.gov.in/Home.aspx
• https://nptel.ac.in/courses/104/106/104106096/
• https://www2.chemistry.msu.edu/faculty/reusch/VirtTxtJml/intro1.htm
• https://nptel.ac.in/courses/104/103/104103071/
• https://www.labster.com/chemistry-virtual-labs/
• https://www.vlab.co.in/broad-area-chemical-sciences