Real-Time PCR Detection System

User Manual
(For X960A/X960B)
Contents
Introduction
P3 How to use this manual

Instructions on Safety and Use

P4 Considerations for Use

Section I - Instrument Installation

P6 Locating the Instrument
P8 Installation of Instrument
P9 Connection of Instrument

Section II - Instrument Appearance and Internal

Components
P10 Overview
P11 Construction & Function

Section III - Instrument Description and

Performance Properties
P14 Instrument Description
P15 Instrument Performance Indicators
P16 Operating Environment and Storage Condition

Section IV - Use and Installation of Software

P17 Installation Instructions
P18 Computer Setting (Win 7)
P21 Start Installation
P24 Overview of User Interface
Section V - Test Introduction and Description
P33 Software Connection to Instrument
P34 New Project File
P40 Specific Test Procedure
P50 Test Considerations

Section VI - Other Function Introduction

P52 Grade Calculation and Retrieval
P53 Auxiliary TM Value Calculator
P54 Logs Recording and Retrieval
P55 Other Internal Operational Functions
 Introduction

How to use this manual

About this manual

This manual is intended to solve your any question and technical problem in installation,
operation and maintenance of this equipment, and provides instructions on proper
installation, use and maintenance of this instrument.

Safety Cautions

Before installation, use and maintenance of this instrument, read and observe any cautions
and considerations in this guide to ensure normal operation of the equipment and user's
personal and property safety.
 Instructions on Safety and Use

Considerations for Use

Operation Safety
● The power supply for this instrument must be earthed reliably. A three-pole
plug is provided for this instrument, with the additional pole connected to
earthing wire, which shall be used with an earthed power socket. Before
connecting power supply, ensure it meets the voltage requirement for this
instrument, and make sure rated load of the power socket is not lower than
the load requirement for this instrument.

● In case of any damage to the power cable, replace it with a new one of the
same model and specification. No object shall be placed onto the power
cable. The cable shall not be located at area subject to busy traffic of persons.
Connect/disconnect the power cable by holding the plug rather than any
portion of the cable itself.

● As high heat will be generated during operation of the instrument, do not

touch any metal module of the instrument with any part of body to prevent
burn.

● No object shall be located within an area of 30cm around the instrument to

ensure good heat dissipation during operation.

Maintenance Safety

● Modules of this instrument shall be maintained to ensure precision of testis

is recommended to clean the instrument with clean soft cloth soaked with

absolute alcohol. Do not use any corrosive detergent or make any detergent
 enter the instrument to prevent any damage to it.

● Immediately switch off the power supply, stop test and contact your supplier

or any professional service personnel in any of the following cases:

1) Any reagent, water or other liquid enters the instrument.

2) The instrument falls down from an elevation or the enclosure is

damaged.

3) The instrument generates any abnormal noise or pungent smell during

operation.

4) Functions of the instrument vary substantially, such as failure to

start/shut down or operate it normally.

 Section I - Instrument Installation

Locating the Instrument

This instrument must be located properly after being unpacked.

Place the packing case upright in horizontal position, cut off the packing strip around the
case and the adhesive tape sealing the case.

Take out the accessories of the case and store them in a secure place for future use.

Remove EPE foam covered on the instrument, and lift it up by supporting its bottom with
two persons, and then locate it on a proper lab desk. Handle the instrument with caution.

Store the packing case properly for containing in future.

 Risk of personal injury! Unless you have been trained properly, do not attempt
to lift the instrument or any heavy component of it. Lifting the packing case in any
improper way may cause ache in or permanent injury to body, or fall of the instrument.
Lift or move the instrument with proper handling tool or in a correct way. Two persons
must be used to lift the instrument up.

This instrument shall be mounted upright on a horizontal surface, around

which there shall not be any vibration, EMI or any high-inductance equipment (e.g.
refrigerator, centrifuge, and stirrer).
Installation of Instrument

Place the instrument on a testing platform

and then take out the power cable
provided along with it. Connect the power
cable by inserting the plug at one end of
the cable into the socket at the rear of the
instrument, and inserting the plug at the
other end into a power socket earthed
properly.

Do not touch the power cable with wet hand. Do not disconnect the cable
during operation of the instrument. In case of any damage in the power cable, replace
it with an acceptable one. The instrument shall be always connected to a socket
earthed properly. When the power supply is disconnected, connect one end of the
network cable provided to the LAN port at the back of the instrument and the other
end to the LAN port on a PC.

Do not connect power supply until the instrument has been connected to a
PC network cable.
Connection of Instrument

Connect PC to the instrument Connect laptop to the instrument

 Section II - Instrument Appearance and
Internal Components

Overview

① Knob ② Lid ③ Handle ④ USB Port ⑤ Pad Tray ⑥ Operation Indicator LED
⑦ Power Switch ⑧ Power Socket & Fuse ⑨ Air Outlet ⑩ 232 Interface ⑪ LAN Port
⑫ WAN Port ⑬ Air Outlet ⑭ Foot Pad ⑮ Air Inlet
Construction & Function
The lid is operated with a sliding guide to a
range as customized by the user. As shown in
the figure at the right side, you switch the knob
to the point UNLOCK and slide the lid
backwards to load the test tube into the
instrument, and then close the lid by sliding it
forwards and switch the knob to a proper
position to run the program for testing.

The handle provided on the instrument is intended for push/pull. Do not lift up or press
down the handler with force to prevent damage to the lid structure.

When the knob is switched to the point

UNLOCK, the lid can be slide back and
forth for loading/unloading of test tube.
After loading the test tube, close the lid
and rotate the knob clockwise to a proper
position and run the program for testing. It
is recommended to align mark on the
knob with the point LOCK.

Do not slide lid component forwards/backwards when the mark aligns with LOCK to
prevent damage to the lid.
Turn pad tray down clockwise to a
position at an angle of 90° to the
enclosure to open the tray, and then
place a pad on the tray.

For this instrument, the tray moves in a range between 0°~90° to the enclosure; do not
move the tray out of this range to prevent damage to the instrument.

There is a status indicator LED under the front panel, which turns on in any of three colors - blue,
green, and red, respectively indicating Standby, Operating, and Alarm.
To ensure a safe and reliable testing procedure, place this instrument on a flat platform,
and reserve areas of at least 30cm wide
around the four sides of it, so as to make
sure proper ventilation and heat
dissipation.

Do not place any object (e.g.

paper, plastic film) at the air inlet to
prevent it from being absorbed in and
thereby permanent damage to the fan or
cooler and damage to the instrument
resulted from overheating of module
component due to poor ventilation.

Do not place any object at any of the air outlets to prevent overheating and
thereby permanent damage to the instrument resulted from poor ventilation.

Do not place any inflammable, explosive or heat-sensitive material near any

air outlet to prevent property damage or potential accident.
 Section III - Instrument Description and
Performance Properties

Instrument Description
[Product Name]
Real-time fluorescence quantitative PCR unit

[Product Model]
X960A
X960B

[Construction]
It consists of power module, control module, driver module, display module,
heated lid module, sample base module, camera module, light source module, and
optical fiber module.

[Application Range]
It is applicable to polymerase chain reaction (PCR).
Instrument Performance Indicators
Product Name: Real-Time Fluorescence Dosing PCR Unit

Product Model: X960A X960B

Compatible OS: Windows XP/VISTA/Windows 7

Bits: 32/64 32/64

Minimum Memory: 512M 512M

Display Unit: 256MB 256MB

CPU: Pentium IV 1.6GHZ or higher

Overall Dimensions: 590mm (L)*442mm (W)*285mm (H)

Weight: 28.5kg 28.7kg

Connection Pattern: LAN WIFI LAN WIFI

Power Supply: AC 220V 50HZ/60HZ

Input Power: 1000VA 1000VA

Fuse: AC 250V 10A AC 250V 10A

Sample Capacity: 96 holes x 0.2ml 96 holes x 0.2ml

Ultrahigh-luminance mono- Ultrahigh-luminance mono-
Light Source:
color LED color LED
High-sensitivity refrigerator High-sensitivity refrigerator
Detector:
CCD CCD
Dynamic Testing Range: 100-1010 100-1010

Minimum Testing
Single copy Single copy
Template:
Reaction Volume: 15μl-100μl 15μl-100μl

Fluorescence Excitation
470nm-525nm 470nm-612nm
Wavelength:
Fluorescence Detection
525mm-571mm 525nm-678nm
Wavelength:

Channel 1:FAM, SYBR

Channel 2:VIC, HEX, JOE,
Fluorescein and Dye Channel 1:FAM, SYBR
TET, TAMRA
Channel 2:VIC, HEX, JOE, Channel 3:ROX, TEXRAD
Detected: Channel 4:CY5
TET, TAMRA
Channel 5:Reserved for user

Temperature Range: 0℃~100℃ 0℃~100℃

 Heating Rate: ≥5℃/s (45℃—99℃) ≥5℃/s (45℃—99℃)

Cooling Rate: ≥5℃/s (45℃—99℃) ≥5℃/s (45℃—99℃)

At constant 95 ℃ within At constant 95 ℃ within

Temperature Control
Uniformity: 10s:±0.4℃ 10s:±0.4℃
at constant 55 ℃ within at constant 55 ℃ within
Temperature Accuracy: ≤±0.1℃ (45℃≤T≤95℃) ≤±0.1 ℃ （ 45 ℃ ≤T≤95 ℃ ）
Gradient Temperature
1℃—30℃ 1℃—30℃
Width:
Gradient Temperature
30℃—100℃ 30℃—100℃
Range:
Heated Lid Temperature
(Rt +2℃) —110℃ (Rt +2℃) —110℃
Range:
Temperature Control Semi-conductor Semi-conductor
Mode: thermoelectric module thermoelectric module
Temperature Control
Max. 999 cycles Max. 999 cycles
Programming:
Special Functions: Threshold analysis, dissolution curve analysis, grade, and
customized parameters

Operating Environment and Storage Condition

Ambient temperature 10℃~30℃

Operating Environment
Relative humidity ＜70%

Ambient temperature -20℃—55℃

Storage Condition
Relative humidity ＜80%

To ensure Normal Operation of the instrument and accuracy of test result,

be sure to operate/store the instrument in accordance with the specified
operating/storage conditions; or otherwise the instrument may be damaged or
personal injury may be caused to the operator.
 Section IV - Use and Installation
of Software

Installation Instructions

Preparations
Items to be prepared: instrument, PC, and connecting wires

Installation Environment
Note: A genuine edition of windows XP/VISTA/7/10 shall be used, or
otherwise any compatibility error may occur. Shut down any anti-
virus software running in the computer during installation to prevent
any false alarm (which shall be ignored if occurring). Shut down any
firewall installed in the computer.

Installation Considerations

Note: To avoid failure of installation resulted from any error occurring

during installation and facilitate your installation, please observe the
following procedure.

Before Start
Note: Before installation of the software, set your computer properly to
prevent interruption of test and thereby data loss.

Note:Do not install on system disk.

Note:Cable and wireless networks can only choose one!

Computer Setting (Win 7)
Display Setting:

1. Navigate to Start > Control Panel.

2. Select "Power Options" in Control Panel.

3. Select "Change when the computer sleeps" in the window

4. Select "Never" under "Turn off the display" in the window
Select "Never" under "Put the computer to sleep".

5. Return to the window in Step 3 and click on "Personalization" button.

6. Click on "Screen Saver" at the lower right corner.

Single click

7. Select "None" from the drop down list in the dialog box.
Start Installation

Step 1: Access the setup package from

the flash disk provided.

Step 2: Double click on the setup file

and choose a proper installation

language.

Step 3: Read the pre-installation

instructions.

Step 4: Choose a destination folder to

install the program.
Step 5: Choose name of the folder to be
created under start menu.

Step 6: Choose whether to create a

shortcut on desktop.

Step 7: Confirm any information before

installation

Step 8: Install the program.

 Step 9: Finish the setup wizard, and
check whether RealGen is running;
check the option and click on Finish.

Step 10: Open page of RealGen.

Step 11: Click on Yes for automatic

connection.

Step 12: If it is indicated that there is

no available computer to connect,
check whether the computer has been
started and the network is operated
properly, or the instruction has been
connected to any other computer.

If the instrument connection fails, check whether it has been properly connected to the computer;
if yes, shut down any anti-virus software and restart the instrument, and then try connection after it beeps. If
connection fails again, contact the dealer for a solution.
Overview of User Interface

Interface Overview

Toolbar - File:

Load Test: Load a finished test file for display and analysis.

Export Test: Export a finished test for data storage.

Load Project: Load a finished project file to start a test.

Export Project: Export a loaded project for data storage.

Import Standard Curve: Import a standard curve with similar circumstance for analysis.

Running Logs: Display any projects running currently in the program.

Print: Print desired information.

Patient Information: Import patient information for printing and data analysis.
Toolbar - Control:
Disconnect: Disconnect the program from the instrument

Connect: Manually connect the program to the instrument by entering its IP address (the default is
"192.168.1.6”").

Pause: Pause operation of the project, which will be resumed in 5 minutes if no operation has been
performed.

Stop: Cease operation of the project.

Operate:

1. Resume a project which is paused, or

2. Operate a chosen project when the instrument is idle.

Toolbar - Interface:
Monitor: Switch to real-time monitoring page.

Analyze: Switch to subsequent analysis page (note: this makes sense only after a test file has been
loaded).

Toolbar - Project:
Change parameters: After a test has been loaded, when current settings are not proper to the
expectation, they may be changed under this option. Any change will be applied immediately after
completion. Change to parameters makes sense only after a test has been loaded.

Project Wizard: Call a project wizard dialog box

Toolbar - Utilities:
TM calculator: Call a TM calculator

Gradient calculator: Call a gradient calculator.

Chinese-English toggle: Toggle between Chinese and English language.

Toolbar - Help:
Instructions: Open instructions in the program
Project Wizard Interface:

New Project: Create a new project for test.

Project Management: Open a project list and manage any project in it, and operate or modify a
project.

Open Test File: Open a test file list and manage any test file, and load or delete a file.

Instrument List: Open an instrument list, and connect or rename any instrument in it.

Software Version: Display current software version number.

Current Address: Display IP address of the computer.

New Project = Basic Settings

Project Name: Name the project to be saved.

Creator: Specify a creator for the project, and save it by pressing ENTER. This option can be left
blank.

Reviewer: Specify a reviewer for the project, and save it by pressing ENTER. This option can be
left blank.

Password: Specify a password to access this project. It can be left blank.

Confirm the password: Confirm the password by repeating it.

Test Result Path: The path where the test result is saved.

Project File Path: The path where the project file is saved.

Test Type: Specify the type of current test.

Channel Selection: Select channels required for current test. At least one channel is required.

Cross-Interference: Call cross-interference setting page to compensate interference between

multi-channel tests.

General Settings: Call general setting page to perform some basic settings.
New Project - Sample Settings

Import: Import sample settings saved already.

Export: Export any sample information edited already for test next time.

Standard: Mark a certain hole position as Standard for a known concentration. This option
should be used along with sample concentration.

Negative: Negative comparison type

Positive: Positive comparison type

Unknown: Sample type in unknown concentration

Gap: Gap comparison type, where water is generally used for comparison.

Unused: No sample is used for current hole position

Sample Concentration: Specify sample concentration for standard sample

Concentration Multiply/ Ascending/ Descending: Quickly mark hole positions arranging in

rows or columns.
Gene Name: Identify the project for the hole position

Sample Name: Identify the sample for the hole position

Housekeeping Gene: Identify housekeeping gene for current test

Standard Sample: Identify standard sample in contrast group for current test

Project Group: Divide projects into different groups

New Project - Sample Settings

Import: Import any program setting file saved already

Export: Export a program setting file edited currently

Low-Temperature Storage: For storage at low temperature upon completion of test

Solution Curve: Enable solution curve function

Save: Save current project file upon completion of test

Operate: Operate any project file saved when the instrument is connected and idle.
Analysis Interface

Introduction
It is used for analysis of test result after completion of test, including baseline setting,

threshold analysis, outcome analysis, solubility curve, and quantitative

analysis. .
 Section V - Test Introduction and
Description

Software Connection to Instrument

Step 1: Power the instrument on, and

connect it to a PC by cabled or

wireless means. Open the program

when the instrument beeps, and the

following information should be

displayed. If not, refresh the instrument

list by using right button.

Step 2: Click on Yes to connect it

directly, or manually connect it in

toolbar.
New Project File

Click on New Project in project wizard

dialog box.

Enter basic setting page. Choose and

enter proper settings and then click on
Next.
 As required by the test, choose and
mark desired hole positions for each
test type.

In case of absolute quantitative test,

hole position must be marked, or
otherwise standard curve will be
unavailable, and there are at least
three standard samples in gradient.
You may import any existing hole
setting file. Quickly mark hole
positions arranging in rows or
columns with concentration
multiply/ascending/descending.
 In case of relative quantitative test,
gene and sample for each desired hole
shall be identified, and housekeeping
gene and reference sample shall be
marked for the test.

When necessary, project groups can be

identified for distinction.

Upon completion, click on Next

Button Function Description:

Add a temperature point

Change parameters for the temperature

point chosen

Set the selected temperature point as

cycle start point

Set the selected temperature point as

cycle end point

Delete a temperature point chosen

Change cycle number. Change or specify

cycle number by dragging from a
temperature point to an end point.
Temperature: Temperature set for a temperature point.

Time: Duration for a temperature point

Temperature variation: Increase temperature variation for each cycle, which will be reflected to
the point in each cycle.

Gradient Width: Modular gradient can be used, and the whole module temperature will be
distributed in gradient.

Time Variation: Increase time variation for each cycle, which will be reflected to the point in each
cycle.

Heating Rate: Change heating rate, and the default is Fastest

Cooling Rate: Change cooling rate, and the default is Fastest

Collect Fluorescence: Mark it to collect fluorescence at current temperature point. Only one
temperature point can be specified for each test. Set it by referring to related agent instructions.

Add at the end: Check it to add the temperature point to the end of the program.
Created or changed temperature control
program. The small blue point indicates
the fluorescence strength in the agent
finally collected at current temperature
point.

Import existing temperature control

program export a compiled temperature

control program

Set whether low-temperature storage is

enabled and the storage temperature.

This option is disabled by default.

Solubility Setting: It is recommended

to use the default value. It is used to

enable or disable solubility test, which

is settable in customization. Save the

project.
Specific Test Procedure

Locate reagent

First align the mark on the knob with point unlock,

and then slide heated lid to put sample reagent into
Hole 96 and then close the lid; rotate the knob
clockwise to a proper position to run the program for
testing. It is recommended to align mark on the knob
with the point LOCK.

Features of Real-Time Quantitative PCR

Specificity: Quantitative PCR features both primer and probe, so is more special than

conventional PCR.

Sensitivity: Sensitivity of fluorescence PCR is up to 10² copies/ml, and the range of linearity in

logarithmic phase analysis is 0~1011 copies/ml. Generally, the number of pathogens in a

clinical specimen is 0~1010/copy, within which quantitative PCR will dose accurately and the

specimen need not be diluted.

Repeatability: Quantitative PCR presents a stable result, since the threshold value is set in

exponential amplification phase, during which, concentration of the reaction constituents is

relative stable, free of side effect, and the logarithm of CT and fluorescence signal are in a

linear relationship. Compared to Endpoint Method CT value is more stable and more

accurately reflects copies of initial template.

Safety: Without post-PCR operation steps, it reduces risk of pollution by the products.
Running Program
A project file can be run by either of

two ways:

1.Click on Control > Run to run a project

file saved in a folder.

2.Choose project management in project

wizard and then choose the target program

and right-click on Run.

After being started, the software module

will display module information, lid
information and estimated time.

After being started, the software module

will display the increasing temperature
for current project.

After being started, the software module

will display the increasing temperature
curve for current project.
After being started, the software module
will display the increasing fluorescence
variation for current project.

When solubility curve is contained in

the project, the software module will

display current temperature variation.

When solubility curve is contained in

the project, the software module will

display current fluorescence variation.

As necessary, you can selectively

review channels and holes

To view detailed output data for the
fluorescence point, move the cursor to
a point on the curve to pop up a box
indicating detailed parameters.

Upon completion of test, the test file

will be automatically saved to the

directory as set for the project.

Test results are classified into result

with CT value, absolute quantitative
result with standard curve, relative
quantitative result with histogram,
SNP result with three pile diagram,
and HRM result with differential
diagram.
Test Analysis Operations

Principle and Application of Quantitative PCR

Real-Time Quantitative PCR is a method which adds fluorescence radical to PCR

reaction system and perform real-time monitor of the whole PCR process by using
fluorescence signals, and carry on quantitative analysis on the unknown template
through s standard curve.

Click on Project > Change Parameters to

choose a location where agent is located

and mark it.

During operation, user can perform

manual settings based reagent
property in Reagent Parameters
Setting at the right side, including:
agent property, sample concentration,
concentration multiple, channel, gene
name marking, sample name marking,
housekeeping gene settings, and
standard sample settings.
 In analysis interface logarithm curve,

logarithm curve drawing is available

and baseline can be re-positioned to

achieve a more suitable CT value.

Baseline
Baseline is a straight line along which fluorescence signals vary slightly in the first
several cycles of PCR amplification reactions.

In analysis interface, you can

choose different channels, and

make analysis by manually

setting baseline or review curves

of a certain type.
 In this table you can view CT
and TM values of a certain curve
and other result information.

CT Value
Means the cycles which fluorescence signals in each PCR reaction tube undergo to
reach the Threshold value as set. According to a research, CT value of each template
is in a linear relation to the logarithm of initial copies of this template. The more
initial copies, the fewer CT value, vice versa. A standard curve can be created by
using a standard product in known initial copies, where the abscissa represents
logarithm of initial copies, and the ordinate indicates CT value. Therefore, initial
copies for an unknown sample can be calculated from the standard curve only by
determining CT value of the sample.

Fluorescence Threshold Setting

Generally fluorescence signals in the first 15 cycles in PCR reaction are
used as fluorescence background signals. Fluorescence Threshold value is
10 times of standard deviation of fluorescence signals in 3~15 cycles, and
set in the exponential phase of PCR amplification.
Absolute Quantitative Analysis - Standard Curve: Absolute Quantitative Analysis
is available when standard samples are contained in test analysis and proper
concentration in three or more gradients has been set for them.

The standard curve can be imported or exported in the interface.

Parameters Related to Standard Curve

Standard samples are required in real-time absolute quantitative PCR test. The

sample used to create standard curve can be in particle.

Determination of molecular weight of standard sample： Concentration of DNA

sample is 50 μg/ml when OD260 value is 1, based on the following formula:

Concentration of sample particle (μg/μl) = OD260 value × nuclein dilution

factor × 50/1000
Relative Quantitative Analysis - Analysis Method: 2  Ct As the most classic relative
quantitative analysis, it is mostly used in release of test report or result. Relative
quantitative analysis is available when gene and sample names are specified in test
analysis and housekeeping gene and standard samples have been set.

Description of relative quantitative test

In relative quantitative test, gene and sample names need to be specified, and

housekeeping gene and standard samples need to be marked. In lab test, this

method is mostly used in final test analysis.

Where error analysis is required, each gene and sample need to be re-tested

based on the condition above.

 Absolute light intensity in solubility
analysis: Where solubility test is
contained in initial test, the software
will draw absolute light intensity for
current solubility test, and the testing
personnel will visually observe
variation of fluorescence intensity
during test.

Relative light intensity in solubility

analysis: As determined from the
absolute light intensity above,
fluorescence value variation trend is
clearly observed during solubility test.
The red line in the diagram will help
testing personnel in positioning the
temperature point with the most
intensive variation of fluorescence
strength.

As shown in information on solubility

test, testing personnel can quickly
search for specific TM value for each
hole in the table.

Description of solubility test

In QPCR test by dye method, solubility test is generally required. Test by dye

method has poor specificity, so solubility test will be performed to validate

whether primer dimer which is not concerns of the test is produced in test.
Instructions on Amplification Curve

Determine whether an amplification curve is acceptable based

on the following:

Clear knee points, particularly distinctive low-concentration sample exponential

phase. Parallel amplification curve as a whole, flat baseline without rise, and

distinctive low-concentration sample exponential phase.

Slope of exponential phase in direct proportion to amplification efficiency - the

more slope, the higher amplification efficiency.

Standard flat and straight baseline or with slight descent, but without obvious

rise trend.

Test Considerations

● Rules and regulations issued by the authorities shall be complied with by the

laboratory.

● Fluorescent material-free disposable gloves, disposable dedicated centrifugal

tube, self-removing pipette and suction head with filter element shall be used.

● Prepare and handle reaction fluid in such way to avoid generation of air bubble;

check whether reaction tube is sealed tightly prior to startup to prevent leakage

of fluorescent material and thereby pollution to the instrument.

● During loading sample soak the sample entirely into the reaction fluid and

prevent attachment of any sample onto the tube wall; seal the tube after loading.

● Take the reaction tube out immediately after completion of amplification, and

seal it in dedicated plastic bag and keep the bag at specified location for

approval and treatment.

● Suction head used in test shall be put in waste jug containing sodium

hypochlorite of 1%, and reject it after sterilization with other waste.

● The test platform and all test appliances shall be disinfected with sodium

hypochlorite of 1%, alcohol of 75% or UV lamp.

● PCR reaction mixed liquor shall be stored at low temperature and protected from

sunlight.

● The test platform shall be cleaned after completion of test to avoid

contamination.
 Section VI - Other Function
Introduction

Grade Calculation and Retrieval

About Grade Functions

Click on "Grade" from main menu to access View Grade page. For convenience
of user, the software provides a grade table for user's retrieval. Upon entry of
central temperature and gradient width, the software will automatically calculate
specific temperature value for each column and helps to ensure accurate test
result.
Auxiliary TM Value Calculator

About TM Value Calculation

Click on "TM" from main menu to access TM Calculation page. Enter a value
and then click on "Calculate" to output the result.
The program is furnished with an embedded TM value calculator for user's access
when necessary.
Logs Recording and Retrieval

About Logs

Click on "Logs" from the main menu to access Logs page and view test operation
logs.
Facilitate management of test history records by user and retrieval of related test
history records.
 Other Internal Operational Functions

Chinese-English Toggle Button

Click on Tool > Chinese or English to

change the language.

Load test data.

Click on “File > Load Test” in main

menu.

In the popup dialog box, select the

target data (in format of .jgtest) stored
previously to load.
 After loading the data, you can

perform relate test analysis on the

loaded data.

Real-Time Modification to Operation Parameters

During operation of the program,

double click on a temperature
segment to modify a parameter in the
popup dialog box.

Modification can be made only by

double clicking on a temperature
segment currently not in operation,
or otherwise a message box will
occurs, indicating "No modification
can be made to any node currently in
operation".
Manufacturer: Heal Force Group -
Hangzhou Jingle Scientific Instrument
Company Limited

Address: Room 401, 4th floor, building 1,

No. 11, Xiyuan 8th Road, Xihu District,

Hangzhou City, Zhejiang Province

Telephone: (0571)89905515

Facsimile: (0571)89905515 - 802

Postal Code: 310000

Email:shanghai@healforce.com

Web: www.healforce.com

Registration Certificate No:

20183220447

Production license No:

20120017

Invention patent No:

ZL 2017 1 0201257.7

Appearance patent No:

ZL 2017 3 0100120.3

Utility model patent No:

ZL 2017 2 0327476.5