Scribd
Upload
11 views38 pages

Microscope M 2

The document outlines the seven essential functions of living organisms, including metabolism and reproduction, and provides guidelines for using a microscope safely. It details the parts of a microscope, how to prepare a slide, and the history and types of microscopes, such as compound light and electron microscopes. Additionally, it explains magnification calculations and the proper techniques for observing specimens.

Uploaded by

aleksandrasolovyev5
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as ODP, PDF, TXT or read online on Scribd
11 views38 pages

Microscope M 2

The document outlines the seven essential functions of living organisms, including metabolism and reproduction, and provides guidelines for using a microscope safely. It details the parts of a microscope, how to prepare a slide, and the history and types of microscopes, such as compound light and electron microscopes. Additionally, it explains magnification calculations and the proper techniques for observing specimens.

Uploaded by

aleksandrasolovyev5
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as ODP, PDF, TXT or read online on Scribd
You are on page 1/ 38

Microsope m

• All living things carry out seven basic functions integral to


survival:
• Metabolism – Living things undertake essential chemical
reactions
• Reproduction – Living things produce offspring, either
sexually or asexually
• Sensitivity – Living things are responsive to internal and
external stimuli
• Homeostasis – Living things maintain a stable internal
environment
• Excretion – Living things exhibit the removal of waste
products
• Nutrition – Living things exchange materials and gases
with the environment
• Growth / Movement – Living things can move and
change shape or size
•
Rules of using a microscope
• Always carry with 2 hands
• Only use lens paper for cleaning
• Do not force knobs
• Always store covered
• Be careful of the cords
Parts of a microscope
Parts of a microscope
• Eyepiece
Parts of a microscope
• Body tube
Parts of a microscope
• Nosepiece
Parts of a microscope
• Objectives
• Low power (short)
• High power (long)
Parts of a microscope
• Arm
Parts of a microscope
• Stage
Parts of a microscope
• Stage clips
Parts of a microscope
• Diaphragm
Parts of a microscope
• Coarse and fine
adjustment knobs
Parts of a microscope
• Light source
Parts of a microscope
• Base
Magnification
• Multiply the eyepiece magnification (10X) by the
objective magnification (4X, 10X, 40X)
• Example: 4 x 10 = 40X total
How to use a microscope
• Place the slide on the stage
• Use stage clips to secure slide
• Adjust nosepiece to lowest setting
• (Lowest = shortest objective)
• Look into eyepiece
• Use coarse focus knob
Slide # 9
How to Prepare a Slide

1. Place slide on a flat surface.


2. Place a drop of water on the slide. Add the specimen to
the drop of water (at times, you may want to have the
specimen already on the slide before adding the water).
3. Hold the coverslip by its sides and lay its bottom edge
on the slide close to the specimen. Holding the coverslip
at a 45° angle helps.
4. Slowly lower the coverslip so that it spreads the water
out. If you get air bubbles (looking like little black
doughnuts), gently press on the coverslip to move them
to the edge. If there are dry areas under the coverslip,
add a little more water at the edge of the coverslip. Too
much water can be dabbed off with a piece of paper
towel
Drawing
History of the Microscope

•1590 –first compound


microscope
History of the Microscope

•1655 – Robert Hooke


used a compound
microscope to observe
pores in cork
•He called them “cells”
Types of Microscopes
•1. Compound Light
Microscope
•1st type of microscope,
most widely used
•light passes through 2
lenses
•Can magnify up to
2000x
Ocular
lens

Objective
lenses
Types of Microscopes
• 2. Electron Microscope
• Used to observe VERY
small objects: viruses,
DNA, parts of cells
• Uses beams of electrons
rather than light
• Much more powerful
•
Types of Microscopes

•Scanning Electron
Microscope (SEM)
•Can magnify up to
100,000x
Scanning Electron Microscopes

Scanning electron microscopes use electrons to probe the sample.


Note that there are no lenses or eyepieces on this SEM. Images are
created from measurements of how the electrons scatter off the
sample.

Image: Grant Institute of Earth Science, University of Edinburgh


Types of Microscopes

•Transmission Electron
Microscope (TEM)
•Can magnify up to
250,000x

You might also like

pFad - Phonifier reborn

Pfad - The Proxy pFad of © 2024 Garber Painting. All rights reserved.

Note: This service is not intended for secure transactions such as banking, social media, email, or purchasing. Use at your own risk. We assume no liability whatsoever for broken pages.


Alternative Proxies:

Alternative Proxy

pFad Proxy

pFad v3 Proxy

pFad v4 Proxy