Operating manual

ProLab 3000

pH/ISE meter
with automatic sensor recognition
and electronic access control

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DRAFT January 28, 2010 2:34 pm
 Accuracy when The use of advanced technology and the high quality standard of our
going to press instruments are the result of a continuous development. This may
result in differences between this operating manual and your instru-
ment. Also, we cannot guarantee that there are absolutely no errors in
this manual. Therefore, we are sure you will understand that we cannot
accept any legal claims resulting from the data, figures or descriptions.

Warranty We guarantee the meter described for 3 years from the date of pur-
chase.
The meter warranty covers manufacturing faults that are discovered
within the warranty period.
The warranty does not cover components that are replaced during
maintenance work, e. g. batteries.

The warranty claim extends to restoring the instrument to readiness for

use but not, however, to any further claim for damages. Improper han-
dling or unauthorized opening of the meter invalidates any warranty
claim.

To ascertain the warranty liability, return the instrument and proof of

purchase together with the date of purchase freight paid or prepaid.

CE conformity SI Analytics GmbH hereby declares that the ProLab 3000 meter is in
Radio data transmission compliance with the basic requirements and the other relevant regula-
tions of the directive 1999/5/EC.
The EC declaration of conformity can be requested from SI Analytics
GmbH.

Copyright © 2010, SI Analytics GmbH

Reprinting - even in the form of excerpts - is only allowed with the
explicit written authorization of SI Analytics GmbH.
Printed in Germany.
ProLab 3000

KONFORMITÄTSERKLÄRUNG
DECLARATION OF CONFORMITY
DÉCLARATION DE CONFORMITÉ

Wir erklären in alleiniger We declare under our sole Nous déclarons sous notre
Verantwortung, dass das responsibility that the seule responsabilité que le
Produkt product produit

pH-/ISE-Messgerät pH / ISE measuring Appareil de mesure

ProLab 3000 instrument pour pH/ISE
ProLab 3000 ProLab 3000
auf das sich diese Erklärung
bezieht, übereinstimmt mit to which this declaration auquel se réfère cette
den Angaben im Kapitel relates is in conformity with déclaration est conforme aux
the specifications in the indications du chapitre
chapter

Technische Daten
pH-/ISE-Messgerät ProLab 3000
30. Oktober 2009

SI Analytics GmbH
Hattenbergstr. 10
D-55122 Mainz
Deutschland, Germany, Allemagne

30. Oktober, October 30, 30 octobre 2005

AGQSF 0000-A107-01/091030

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 ProLab 3000

4 ba75645e02 01/2010
ProLab 3000 Contents

ProLab 3000 - Contents

1 Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
1.1 General features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
1.2 Keypad . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
1.3 Display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
1.4 Socket field . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
1.5 Automatic sensor recognition . . . . . . . . . . . . . . . . . . . . . 17
1.5.1 ID sensors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
1.5.2 Sensor data from ID sensors . . . . . . . . . . . . . . . 19
1.6 Electronic access control . . . . . . . . . . . . . . . . . . . . . . . . 20

2 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
2.1 Authorized use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
2.2 General safety instructions . . . . . . . . . . . . . . . . . . . . . . . 22

3 Commissioning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
3.1 Scope of delivery. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
3.2 Initial commissioning . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
3.3 Connecting the power pack. . . . . . . . . . . . . . . . . . . . . . . 24
3.4 Switching on the ProLab 3000 . . . . . . . . . . . . . . . . . . . . 25
3.5 Connecting the USB mouse . . . . . . . . . . . . . . . . . . . . . . 26
3.6 Connecting sensors . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
3.6.1 Connecting an ID sensor . . . . . . . . . . . . . . . . . . 27
3.6.2 Connecting a non ID sensor. . . . . . . . . . . . . . . . 28
3.7 Connecting optional accessories . . . . . . . . . . . . . . . . . . 28
3.7.1 RS232 interface (serial port) . . . . . . . . . . . . . . . 29
3.7.2 USB-B interface (USB Device). . . . . . . . . . . . . . 30
3.7.3 USB-A interface (USB Host) . . . . . . . . . . . . . . . 31

4 Operating principles . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
4.1 Operating and display elements . . . . . . . . . . . . . . . . . . . 33
4.2 File system . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
4.3 Entry of numerals, letters and characters . . . . . . . . . . . . 37
4.4 Navigation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
4.4.1 Navigation in the measured value display . . . . . 40
4.4.2 Navigation in menus. . . . . . . . . . . . . . . . . . . . . . 41
4.4.3 Navigation in dialog boxes . . . . . . . . . . . . . . . . . 43
4.4.4 Navigation in the file selection dialog box . . . . . 44

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5 Access to the meter . . . . . . . . . . . . . . . . . . . . . . . . . . . 47

5.1 Switch the meter on and off . . . . . . . . . . . . . . . . . . . . . . . 47
5.2 Login as a user . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
5.3 Password for login . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
5.3.1 Changing the password . . . . . . . . . . . . . . . . . . . 49
5.3.2 Assigning a password. . . . . . . . . . . . . . . . . . . . . 49
5.3.3 Forgotten the password? . . . . . . . . . . . . . . . . . . 49
5.4 Lock . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
5.5 Access control and user rights. . . . . . . . . . . . . . . . . . . . . 51
5.5.1 Access control . . . . . . . . . . . . . . . . . . . . . . . . . . 52
5.5.2 User management and assigning user rights . . . 53
5.6 Lost your electronic key? . . . . . . . . . . . . . . . . . . . . . . . . . 55

6 System functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
6.1 Overview: System settings . . . . . . . . . . . . . . . . . . . . . . . 56
6.2 Selecting the language . . . . . . . . . . . . . . . . . . . . . . . . . . 58
6.3 Selecting the country . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
6.4 Setting the date and time . . . . . . . . . . . . . . . . . . . . . . . . . 59
6.5 Selecting the channels for measured value display. . . . . 60
6.6 Interfaces for data download . . . . . . . . . . . . . . . . . . . . . . 60
6.7 Temperature . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
6.7.1 Temperature unit . . . . . . . . . . . . . . . . . . . . . . . . 61
6.7.2 Temperature measurement . . . . . . . . . . . . . . . . 62
6.8 Automatic stability control . . . . . . . . . . . . . . . . . . . . . . . . 63
6.9 Color assignment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
6.10 Reset . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
6.10.1 Resetting the sensor settings . . . . . . . . . . . . . . . 65
6.10.2 Resetting the system settings . . . . . . . . . . . . . . . 65
6.11 List of open windows . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66

7 pH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
7.1 General information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
7.2 Measuring the pH value . . . . . . . . . . . . . . . . . . . . . . . . . . 68
7.2.1 Preparatory activities . . . . . . . . . . . . . . . . . . . . . 68
7.2.2 Measuring . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
7.2.3 Measurement settings . . . . . . . . . . . . . . . . . . . . 71
7.3 Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
7.3.1 Carrying out a calibration procedure
(example: AutoCal DIN) .............. 77
7.3.2 Carrying out a calibration procedure
(example: VariCal) . . . . . . . . . . . . . . . . . . . . . . . 81
7.3.3 Calibration settings and calibration data . . . . . . . 85
7.3.4 Calibration interval . . . . . . . . . . . . . . . . . . . . . . . 86

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8 ORP voltage. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87
8.1 General information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87
8.2 Measuring the ORP. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88
8.2.1 Preparatory activities . . . . . . . . . . . . . . . . . . . . . 88
8.2.2 Measuring . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89
8.2.3 Measuring the relative ORP . . . . . . . . . . . . . . . . 91
8.2.4 Measurement settings . . . . . . . . . . . . . . . . . . . . 94

9 Ion concentration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95
9.1 General information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95
9.2 Measuring the ion concentration . . . . . . . . . . . . . . . . . . 96
9.2.1 Preparatory activities . . . . . . . . . . . . . . . . . . . . . 96
9.2.2 Measuring . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97
9.2.3 Measurement settings . . . . . . . . . . . . . . . . . . . . 99
9.3 Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101
9.3.1 Carrying out calibration . . . . . . . . . . . . . . . . . . 104
9.3.2 Calibration data . . . . . . . . . . . . . . . . . . . . . . . . 108
9.4 Blank value correction. . . . . . . . . . . . . . . . . . . . . . . . . . 109
9.5 Reference measurement. . . . . . . . . . . . . . . . . . . . . . . . 110
9.6 Measuring with increment procedure (methods). . . . . . 111
9.6.1 Selecting the measuring method . . . . . . . . . . . 111
9.6.2 Standard addition . . . . . . . . . . . . . . . . . . . . . . . 112
9.6.3 Double standard addition . . . . . . . . . . . . . . . . . 114
9.6.4 Standard subtraction . . . . . . . . . . . . . . . . . . . . 117
9.6.5 Sample addition . . . . . . . . . . . . . . . . . . . . . . . . 120
9.6.6 Sample subtraction . . . . . . . . . . . . . . . . . . . . . 123
9.6.7 Blank value addition (Standard addition with
blank value correction) . . . . . . . . . . . . . . . . . . . 126

10 Memory . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 129
10.1 Measurement data . . . . . . . . . . . . . . . . . . . . . . . . . . . . 129
10.1.1 Creating and selecting a file for manually
stored measurement datasets . . . . . . . . . . . . . 130
10.1.2 Storing measurement datasets manually. . . . . 131
10.1.3 Automatically storing measurement datasets . 132
10.1.4 Displaying and filtering stored measurement
datasets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 134
10.1.5 Erasing measurement datasets . . . . . . . . . . . . 135
10.2 Calibration data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 136
10.2.1 Displaying stored calibration data . . . . . . . . . . 136
10.2.2 Managing the calibration data . . . . . . . . . . . . . 137
10.3 Recorder data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 138
10.4 Configuration data. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 138

11 Recorder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 139
11.1 Recording the measured value curve . . . . . . . . . . . . . . 140
11.2 Recording the curve of the measured

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temperature value ........................ 141

11.3 Storing recorder data . . . . . . . . . . . . . . . . . . . . . . . . . . . 142
11.4 Displaying stored recorder data. . . . . . . . . . . . . . . . . . . 143
11.5 Settings for the recorder . . . . . . . . . . . . . . . . . . . . . . . . 144
11.5.1 Defining the recording interval . . . . . . . . . . . . . 145
11.5.2 Specifying the axes of the system
of coordinates ..................... 145
11.5.3 Defining and activating limit values. . . . . . . . . . 147
11.5.4 Defining the colors for the
recorder representation ................ 148
11.6 Changing the displayed axis intercept . . . . . . . . . . . . . . 149
11.7 Displaying exact measurement data at a point (cursor) 151
11.8 Deleting stored recorder data . . . . . . . . . . . . . . . . . . . . 151

12 Transmitting data . . . . . . . . . . . . . . . . . . . . . . . . . . . . 153

12.1 Printing current measurement data . . . . . . . . . . . . . . . . 153
12.2 Transmitting data (to a PC or printer) . . . . . . . . . . . . . . 153
12.2.1 RS232 interface . . . . . . . . . . . . . . . . . . . . . . . . 153
12.2.2 USB-B interface (USB Device) . . . . . . . . . . . . . 154
12.2.3 USB-A interface (USB Host) . . . . . . . . . . . . . . . 155
12.2.4 Options for data transmission . . . . . . . . . . . . . . 155
12.3 Data backup. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 156
12.3.1 Data backup by the administrator . . . . . . . . . . . 156
12.3.2 Viewing backed up data . . . . . . . . . . . . . . . . . . 157

13 Configurations. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 158
13.1 Viewing a configuration . . . . . . . . . . . . . . . . . . . . . . . . . 159
13.2 Creating a new configuration . . . . . . . . . . . . . . . . . . . . . 160
13.3 Loading a configuration . . . . . . . . . . . . . . . . . . . . . . . . . 161
13.4 Deleting a configuration . . . . . . . . . . . . . . . . . . . . . . . . . 162

14 Maintenance, cleaning, disposal . . . . . . . . . . . . . . . . 163

14.1 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 163
14.2 Cleaning. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 164
14.3 Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 164

15 What to do if... . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 165

15.1 pH and ORP measurement . . . . . . . . . . . . . . . . . . . . . . 165
15.2 Ion selective measurement . . . . . . . . . . . . . . . . . . . . . . 167
15.3 General errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 168

16 Technical Data. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 169

16.1 General data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 169
16.2 Measuring ranges, resolution, accuracy . . . . . . . . . . . . 172
16.2.1 Temperature . . . . . . . . . . . . . . . . . . . . . . . . . . . 172
16.2.2 pH/ORP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 172
16.2.3 ISE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 173

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17 Lists . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 175

Appendix . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 183
A.18 Firmware update . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 183
A.19 Menus . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 185
A.19.1 Main menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . 185
A.19.2 pH sensor menu. . . . . . . . . . . . . . . . . . . . . . . . 187
A.19.3 U sensor menu. . . . . . . . . . . . . . . . . . . . . . . . . 188
A.19.4 dU sensor menu. . . . . . . . . . . . . . . . . . . . . . . . 188
A.19.5 ISE sensor menu . . . . . . . . . . . . . . . . . . . . . . . 189

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Contents ProLab 3000

10 ba75645e02 01/2010
ProLab 3000 Overview

1 Overview
1.1 General features
The ProLab 3000 precision pH meter enables you to
perform pH and ORP measurements rapidly and reliably.
Das ProLab 3000 provides the maximum degree of ease of use, reli-
ability and, above all, measuring certainty for all applications.

The proven calibration procedures, and

stability control function (SC) and the sensor recognition function sup-
port your work with the meter.

In addition, the ProLab 3000 provides an electronic access control.

Documented measurement data is thus automatically assigned to a
user.

1 Keypad
2 Display
3 Reader field for electronic access con-
trol
4 Jack field

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Overview ProLab 3000

1.2 Keypad

1 ABC 2 DEF 3

GHI 4 JKL 5 MNO 6

MODE CAL SC ESC
OK
PQRS 7 TUV 8 WXYZ 9
STO RCL PRINT
_ . 0 DEL

Keys with dedicated Keys with dedicated functions are labeled on the meter.
functions In this operating manual, they are identified by the key labeling, bold let-
ters and angle brackets <..> .
Keys with changing Keys with changing functions are not labeled; the currently assigned
functions function is shown on the display instead. In this operating manual,
(softkeys) these keys are identified by the displayed softkey function, bold letters
and angle brackets [..] .
The key symbol (e.g. <OK> or [OK]) generally means you should press
a key in this operating manual.

Key functions <On/Off> Switch the meter on/off

<MODE> Select measured parameter

MODE

<CAL> Call up calibration procedure

CAL

<SC> Switch on or off the stability control func-

SC tion manually.

< > Reduce values, Scroll

Move in the menu

< > Increase values, Scroll

Move in the menu

<W> Reduce values, Scroll

Move in the menu
<X> Increase values, Scroll
Move in the menu

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ProLab 3000 Overview

<OK> Confirm entries

OK

<ESC> Return to higher menu level /

ESC Cancel inputs

<STO> Store a measured value

STO

<RCL> Open the menu for stored measured val-

RCL ues

<PRINT> Print or output data to an interface (serial

PRINT RS232, USB-A (USB Host) or USB-B USB
Device))

Alphanumeric keypad The keys of the alphanumeric keypad are used to enter numerals
(<0...9>), letters (<A...Z>) and characters (<- .>). The keys of the alpha-
numeric keypad only have a function if the ProLab 3000 is waiting for a
character to be entered, e.g. in input fields for the ID during manual
storage.

<0...9> Press the key once.

PQRS 7 The numeral is displayed in the input field

<A...Z> Press the key several times if necessary

PQRS 7 until the required letter is displayed in the
input field
<- .> Depending on the input field, the keys is
_ . automatically interpreted as a minus sign
or decimal point.

Numeral input field:

Cursor is in front of the first digit: Minus.
Cursor is behind a numeral: Decimal point
<DEL> Deletes the character left of the cursor
DE L

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Overview ProLab 3000

1.3 Display
The graphic color display displays the measurement data. The illumi-
nation enables to read the display even in the darkness.

11
1

2
10

4
7

6 5

Elements:
1 Menu line (main menu)
2 Sensor window (title bar)
3 Menu line (sensor menu)
4 Status line
5 Info line with date and time, info or action text
6 Key assignment with changing functions (softkeys)
7 Temperature display
8 Measured value (with unit)
9 Measured parameter
10 ID sensor symbol
11 CalClock

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ProLab 3000 Overview

1.4 Socket field

RS232
TP 2 TP 1 USB Device
pH/U/ISE pH/U/ISE
Probe 2 Probe 1 9V/DC
Ref 2 Ref 1 USB Host input

3 6 8 10
1 2 4 5 7 9
Connections:
1 pH/ISE/ORP electrode (pH/U/ISE Probe 2)
2 Reference electrode (Ref 2)
3 Temperature sensor (TP 2)
4 pH/ISE/ORP electrode (pH/U/ISE Probe 1)
5 Reference electrode (Ref 1)
6 Temperature sensor (TP 1)
7 USB-A interface (USB Host), e.g. for
– the USB mouse,
– a USB printer,
– a USB hub,
– a USB keyboard,
– a USB storage,
– a USB card reader or
– other USB devices
8 USB-B interface (USB Device),
e.g. for a PC.
9 Power pack (9V/DC input)
10 RS232 interface (RS232)

RS232
TP 2 TP 1 USB Device
Cond pH/U/ISE pH/U/ISE
Probe Probe 2 Probe 1 9V/DC
Ref 2 Ref 1 USB Host input

4 7 9 11
1 2 3 5 6 8 10

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Overview ProLab 3000

CAUTION
Only connect sensors to the meter that cannot return any voltag-
es or currents that are not allowed (> SELV and > current circuit
with current limiting).
Almost all sensors - in particular SI Analytics sensors - fulfill
these conditions.

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ProLab 3000 Overview

1.5 Automatic sensor recognition

The automatic sensor recognition function enables
z to operate a sensor with different meters without recalibrating
z to operate different sensors with one meter without recalibrating
z to assign measurement data to a sensor
– measurement datasets are always downloaded to the interface
with the sensor type and sensor series number.
– measurement datasets are always stored together with the sen-
sor type and sensor series number.
z to assign calibration data to a sensor
– calibration data is always downloaded to the interface with the
sensor type and sensor series number.
z to hide menus that are not relevant for this sensor

To be able to use the automatic sensor recognition function a meter is

required that supports the automatic sensor recognition (e.g.
ProLab 3000), and a sensor (ID sensor) that is suitable for sensor rec-
ognition.

In every ID sensor, sensor data is stored that clearly identifies the sen-
sor.
DIN The sensor data is sent to the meter automatically via radio commu-
nication and used for sensor identification there.

BNC

Note
With the ProLab 3000 you can also operate non ID sensors. Then,
however, you cannot use the advantages of the sensor recognition
function.

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Overview ProLab 3000

1.5.1 ID sensors

SI Analytics GmbH ID sensors support the automatic sensor recogni-

tion function. Their sensor designation has the addition "ID", e.g. Blue-
Line A 161 1M-BNC-ID electrode.

Note
Information on available ID sensors is given on the Internet or directly
by SI Analytics.

ID sensors connected to the ProLab 3000 meter can be recognized by

the ID sensor symbol on the display.

ID sen-
sor sym-
bol

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ProLab 3000 Overview

1.5.2 Sensor data from ID sensors

ID sensors transmit the following sensor data to the meter:

z SENSOR ID
– Sensor type
– Sensor series number
z Calibration data
– Calibration date
– User (having carried out the last calibration procedure)
– Calibration characteristics
– Calibration interval
– Selected buffer set (pH electrodes only)
The calibration data is updated in the ID sensor after each calibration
procedure. A message is displayed while data is updated in the sensor.

Note
While data is updated in the sensor, the sensor must not be discon-
nected because otherwise the calibration data will not be completely
transmitted. The sensor will then have no valid calibration.

Note
If non-ID sensors are used, the calibration data from the meter is used
and also stored in the meter.

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Overview ProLab 3000

1.6 Electronic access control

The ProLab 3000 always documents measurement and calibration
data together with a user name.
Thus all measurement data is assigned to the user, in compliance with
GLP.

The user name is easily and safely transmitted to the ProLab 3000 via
an electronic key. Each electronic key in the form of a keyring pendant
contains a key number and a user name. The key number and user
name are read by the meter via a contactless radio data connection.

The meter checks the access authorization for the user name. Mea-
surements with an electronic key are only possible if the key number
and user name is registered in the meter. The measuring data is then
documented along with the registered user name.

Reader field for

the electronic
key

If the user name of an electronic key is not registered in the meter,

access to the meter with this electronic key is denied.

If access to the meter is anonymous (no password, no electronic key

required), all data are documented with the user name, Anonymous.
By labeling the measurement data with Anonymous, this data can be
excluded from the GLP-compliant documentation.

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ProLab 3000 Safety

2 Safety
This operating manual contains basic instructions that you must follow
during the commissioning, operation and maintenance of the meter.
Consequently, all responsible personnel must read this operating man-
ual before working with the measuring system. The operating manual
must always be available within the vicinity of the meter.

Target group The meter was developed for work in the laboratory.
Thus, we assume that, as a result of their professional training and
experience, the operators will know the necessary safety precautions
to take when handling chemicals.

Safety instructions The individual chapters of this operating manual use the following
safety instruction to indicate various types of danger:

CAUTION
indicates instructions that must be followed precisely in order to
avoid the possibility of slight injuries or damage to the meter or
the environment.

Further notes

Note
indicates notes that draw your attention to special features.

Note
indicates cross-references to other documents, e.g. operating manu-
als.

2.1 Authorized use

This meter is authorized exclusively for pH, ISE and ORP measure-
ments in the laboratory.
The technical specifications as given in chapter 16 TECHNICAL DATA
(page 169) must be observed. Only the operation and running of the
meter according to the instructions given in this operating manual is
authorized. Any other use is considered unauthorized.

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Safety ProLab 3000

2.2 General safety instructions

This instrument is built and inspected according to the relevant guide-
lines and norms for electronic measuring instruments (see page 169).
It left the factory in a safe and secure technical condition.

Function and The smooth functioning and operational safety of the meter can only be
operational safety guaranteed if the generally applicable safety measures and the specific
safety instructions in this operating manual are followed during opera-
tion.

The smooth functioning and operational safety of the meter can only be
guaranteed under the environmental conditions that are specified in
chapter 16 TECHNICAL DATA (page 169).

If the instrument was transported from a cold environment to a warm

environment, the formation of condensate can lead to the faulty func-
tioning of the instrument. In this event, wait until the temperature of the
meter reaches room temperature before putting the meter back into
operation.

Safe operation If safe operation is no longer possible, the meter must be taken out of
service and secured against inadvertent operation!
Safe operation is no longer possible if the meter:
z has been damaged in transport
z has been stored under adverse conditions for a lengthy period of
time
z is visibly damaged
z no longer operates as described in this manual.

If you are in any doubt, please contact the supplier of the meter.

Obligations of the The purchaser of this meter must ensure that the following laws and
purchaser guidelines are observed when using dangerous substances:
z EEC directives for protective labor legislation
z National protective labor legislation
z Safety regulations
z Safety datasheets of the chemical manufacturers.

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ProLab 3000 Commissioning

3 Commissioning
3.1 Scope of delivery
z ProLab 3000 laboratory meter
z Power pack
z 4 batteries 1.5 V Micro type AAA
z 1 electronic administrator key (as keyring pendant)
z 1 electronic user key (as keyring pendant)
z Cover
z USB cable (Z875)
z USB mouse
z CD-ROM with USB driver for the PC
z Operating manual

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Commissioning ProLab 3000

3.2 Initial commissioning

Perform the following activities:
z Insert the batteries (see page 163)
z Connect the power pack (see page 24).
z Switch on ProLab 3000 (see page 25).
z Connect the USB mouse (see page 26)
z Setting the language (see page 58)
z Set the date and time (see page 59)
z Set up the access authorization for user keys (see page 53)
z Connect the sensor (see page 27).

3.3 Connecting the power pack

The power pack supplies the meter with low voltage (9 V DC).

The batteries are only used to buffer the system time if the power sup-
ply is interrupted.

CAUTION
The line voltage at the operating site must lie within the input
voltage range of the original power pack (see page 169).

CAUTION
Use original power packs only (see page 169).

1 Insert the plug into the socket of the meter.

2 Connect the original power pack to an easily accessible power
outlet.
The self-test, meter designation, software version and subse-
quently the Standby display appear.

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ProLab 3000 Commissioning

3.4 Switching on the ProLab 3000

The ProLab 3000 indicates the Standby display.

1 Switch the meter on with <OK>.

2 The meter switches itself on. The Login dialog box is displayed.

Note
The default password for the administrator is "00001".
For your safety, change it as soon as possible (see page 49).

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Commissioning ProLab 3000

3.5 Connecting the USB mouse

You can connect the USB mouse included in the scope of delivery to
the USB-A USB Host interface of the ProLab 3000. Thus the
ProLab 3000 can be operated like a PC.

3.6 Connecting sensors

If an ID sensor is connected, the ProLab 3000 recognizes the sensor
type, sensor name and series number of the sensor.
If the ID sensor cannot be automatically assigned to a channel, the
Automatic sensor recognition window prompts the manual assignment.

Note
 wrong assignment of ID sensors to channels can be corrected by
reassigning the sensors, e.g. after
z Switching off, then on the meter
z Disconnecting, then reconnecting all ID sensors.

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ProLab 3000 Commissioning

3.6.1 Connecting an ID sensor

ID sensor with
1 Connect the temperature sensor of the ID sensor to the socket.
temperature sensor
2 Connect the ID sensor to the socket of the meter.
The sensor is automatically assigned.
The channel to which is was assigned shows the measurement
data of the sensor.

ID sensor without
1 Connect the ID sensor to the socket of the meter.
temperature sensor
As soon as the meter has recognized the ID sensor, the Auto-
matic sensor recognition window appears with the sensor
name and series number.

2 Assign the sensor that was recognized to a channel with pH/U/

ISE Probe 1 or pH/U/ISE Probe 2.
As soon as a sensor was assigned to a channel, the assign-
ment is completed and the Automatic sensor recognition is
closed again.

Note
The manual assignment of a sensor to a channel is stored in the
meter. The next time the meter is switched on while ID sensors are
connected, the last assignment of the sensor is displayed and can be
confirmed with <OK>.

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Commissioning ProLab 3000

3.6.2 Connecting a non ID sensor

1 Connect the sensor and if necessary, temperature sensor to a

socket.
The relevant channel shows the measurement data of the sen-
sor.
2 Prior to measuring:
Calibrate the sensor.

3.7 Connecting optional accessories

The ProLab 3000 has several interfaces to connect accessories to. To
connect a device, find the suitable interface in the following table.
More information on how to operate an interface is provided with the
description of the interface.

Interface
Accessory RS232 USB-B USB-A
(see (USB Device) (USB Host)
page 29) (see page 30) (see page 31)
PC x x
Printer x x
USB mouse x
USB memory x
USB card reader x
USB keyboard x
USB barcode reader x
USB hub x

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ProLab 3000 Commissioning

3.7.1 RS232 interface (serial port)

PC and printer can be connected via the RS232 interface.

Data that is transmitted to the interface with <PRINT> can be printed
out with a printer or received with a terminal program if a PC is con-
nected.
Operation with RS232 Connect the interface of the ProLab 3000 to the devices using the Z390
(PC) or Z893 (external USB printer Z890) cable.

Set up the following transmission data on the PC/printer:

Baud rate selectable between: 1200 ... 115200

The baud rate must agree with the baud rate set on
the PC/printer.
Handshake RTS/CTS
PC only:
Parity none
Data bits 8
Stop bits 1

Note
Pin assignment of the socket: See page 169.

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Commissioning ProLab 3000

3.7.2 USB-B interface (USB Device)

You can connect a PC to the meter via the USB-B interface(USB

Device).
Data that is transmitted to the interface with <PRINT> can be printed
out with a printer or received with a terminal program if a PC is con-
nected.
The USB driver of the enclosed CD-ROM must be installed for the inter-
face to be available for the PC.

Installation of the USB System requirements of the PC for installation of the USB driver:
driver on the PC z PC with Pentium processor or higher with at least one free USB con-
nection and CD-ROM drive
z Windows 2000, XP.

1 Insert the supplied installation CD in the CD drive of your PC.

2 Connect the meter to the USB input of the PC via the USB
cable.
Windows automatically installs the driver for the meter. Follow
the Windows installation instructions as necessary.
The meter is listed as a virtual COM interface among the con-
nections in the Windows instrument manager.

Operation at USB-B Connect the interface on the ProLab 3000 to the PC using a cable with
(USB Device) a USB-A and a USB-B plug.

Set up the following transmission data on the PC/printer:

Baud rate selectable between: 1200 ... 115200

The baud rate must agree with the baud rate set on
the PC/printer.
Handshake RTS/CTS
PC only:
Parity none
Data bits 8
Stop bits 1

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ProLab 3000 Commissioning

3.7.3 USB-A interface (USB Host)

You can connect the following devices to the USB-A interface (USB
Host) at the ProLab 3000:

Accessory Use
USB mouse Simple operation of the ProLab 3000 like a
PC
USB printer Simple output of data to the printer with the
<PRINT> key
USB memory Simple data backup on an external USB
medium
USB card reader Simple data backup on an external memory
card
USB keyboard Input of text with the keyboard
USB barcode reader Input of data such as sample designations
using a barcode.
Data input using the barcode reader is
always possible whenever it is possible to
input data via the keyboard.
USB hub (USB 2.0) Multiplication of the number of USB-A inter-
faces USB Hostfor simultaneous operation
of several USB devices

Operation at USB-A Connect the interface on the ProLab 3000 to the external device using
(USB Host) a cable with a USB-A and a USB-B plug.
The device is automatically recognized and is immediately operable.

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Commissioning ProLab 3000

32 ba75645e02 01/2010
ProLab 3000 Operating principles

4 Operating principles
This section contains basic information on the operation of the
ProLab 3000.

4.1 Operating and display elements

Mouse The ProLab 3000 is designed for mouse operation. With a mouse click
you can carry out all functions except for the entry of characters and
numerals. This means the ProLab 3000 can be operated as easily as a
software on the PC.

Keys Frequently required functions such as Store (<STO>), Calibrate

(<CAL>) or Print (<PRINT>) are directly available using keys. In this
operating manual, keys with dedicated functions are identified by the
key labeling, bold letters and angle brackets, e.g. <OK>.

Note
When the meter is operated with the mouse, all key functions are
available in a context menu (right mouse button).

Window The screen shows display windows and dialog boxes like a PC soft-
ware. Only one window is active at a time. The active window has a col-
ored title bar. Windows that are not active have a grayed out title bar.
In the measured value display, for each channel there is a window with
special menus for the displayed measured parameter.

Windows contain further operating elements such as buttons, register

cards, selection lists, option fields and input fields.

When operating the meter without the mouse, use the [Tab] softkey to
activate the open window while scrolling, e.g. individual channels or the
main menu.

Buttons Functions in dialog boxes are executed using buttons. In this operating
manual, buttons are identified by angle brackets. Example: [Continue]
or [OK].
When operating without the mouse the buttons have to be selected
first. Then the respective function can be executed with <OK>. The
[Cancel] button can always be operated with the <ESC> key directly.

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Operating principles ProLab 3000

Display Depending on the operating situation, the color display shows mea-
surement data, calibration steps, setting dialogs or stored data.

12

1 Main menu
2 Channel
3 Menu for a measured parameter
5 Info line
6 Softkey assignment
12 Softkeys

In the main menu, there are sensor independent functions and settings:
Main menu (1) z Manage configurations (File ) (see page 158)
z Store current measurement data, and display and edit stored mea-
surement and calibration data (Memory) (see page 129)
z System settings such as language, date, time, color assignment,
interface settings and general measurement settings (System) (see
page 56)
z Select the measurement data to be displayed (Window)
z Manage user data (User ) (see page 139)

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ProLab 3000 Operating principles

Channel (2) In the measured value display, for each connected sensor you can see
a channel with special menus for the displayed measured parameter
(Measuring, Calibration).

Menu for a measured Menus for a measured parameter comprise sensor dependent func-
parameter (3) tions and settings:
z Calibration settings and calibration data (Calibration) (see section
CALIBRATION in the chapter for the respective sensor)
z Temperature settings such as usage of the temperature sensor of
another sensor, manual temperature setting (Measuring)
z The measured value display with recorder (see page 140)
z Resetting the sensor settings
z Special measurement settings (Measuring)
A detailed description of the functions is given with the description of
the measured parameter.

Info line (4) The info line shows the date and time, information or instructions on the
current situation.

Softkey assignment and Softkeys provide additional, situation-related functions. In this operat-
softkeys (5+6) ing manual, softkeys are indicated by the currently displayed function,
bold letters and angle brackets, e.g. [Tab].
The current functions are displayed in the four fields in the lower display
(empty field = no function).

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Operating principles ProLab 3000

4.2 File system

Files With the ProLab 3000 you can store data in files just like using a PC.
Different data is stored in an extra file, such as
z manually stored measured values
z automatically stored measured values
z recorder data

A complete overview of the data that can be stored and the correspond-
ing file formats is given in chapter 10 MEMORY (see page 129).

File system The ProLab 3000 creates an individual directory with the user name for
each registered user.
In his/her directory, each user can:
z Create folders
z Store data
z Delete files
z View stored files

File dialogs such as Save as always suggest the directory of the regis-
tered user.

Note
If an external USB memory is connected, the external memory
appears as a folder in the directory of the user.

Only the administrator can move to a higher directory and in other user
folders.

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ProLab 3000 Operating principles

4.3 Entry of numerals, letters and characters

Numerals, letters, punctuation marks and special characters are
entered on the alphanumeric keypad on the meter or an external key-
board.
Entering characters is required in operating situations such as the fol-
lowing:
z Entry of the date and time
z Entry of an ID, e. g. if you want to store measurement data
z Entry of the user name and password
z User management

Character set The following characters are available:

z Numerals 0 ... 9
z Letters A ... Z
z Punctuation marks . -

Operating principle Characters can be entered as long as an input field is displayed.

The keys of the alphanumeric are assigned to the characters that are
PQRS 7
printed on them. Example: With the <PQRS 7> key you can enter the
following characters: 7, P, Q, R, S.
The required character is selected by pressing the key several times
(similar to a cell phone). The numeral appears on the first pressing if
the key is assigned to several characters. One keypressing is sufficient
to enter a numeral.

The key for punctuation marks (<- .>) is assigned to a decimal point of
minus sign, depending on the context. If a number is in front of the
punctuation mark, a decimal point is automatically displayed, in all
other cases a minus (-).

A character is taken over in the input field if

z the character is highlighted longer than one second,
z the character is confirmed with <OK>,
z another alphanumeric key is pressed.

Operating example: The input field for an ID appears if you press the <STO> key to store
Entering an ID measurement data. A dialog with input fields for the ID and comment
opens up. In the following example, a measurement dataset with the ID
"Test" should be stored.

1 Press <TUV 8> several times until "T" appears in the input line.
After approx. 1 second the character is taken over.

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Operating principles ProLab 3000

2 Complete and confirm the ID with <A...9>.

Mouse operation:
With the mouse, you have in all entry fields the additional option of
showing a keyboard with the right mouse button. You can then enter
letters or numerals with the mouse.

Correcting wrong Move the cursor behind the character to be deleted with <W><X>.
entries Then delete the character in front of the cursor with <DEL>.

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ProLab 3000 Operating principles

4.4 Navigation
The dialog with the meter takes place through menus and dialog boxes
as usual with any PC software. In each dialog box there are operating
elements such as register cards, selection lists, buttons or input fields.

Note
The ProLab 3000 is designed to be operated with a mouse. A suitable
mouse is included in the scope of delivery.

General operating If you wish to operate the meter without using a mouse, find some gen-
principles eral operating principles listed below.

Key / soft- Measured Menu Dialog box

key value display
[Tab] Select main - Select an element
menu or such as a register
channel card or button
[Menu] Open main - -
menu or
selected
channel
<S><T> - Select a menu Select a subele-
and item ment in a dialog
<W><X> box
<OK> - Execute a menu Execute the func-
item, e.g. start an tion of the
action or open a selected button
dialog box.
<ESC> - Close the menu Cancel current
action without
change

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Operating principles ProLab 3000

4.4.1 Navigation in the measured value display

The display shows, for example, the measurement data of all activated
channels, the main menu, the sensor menu and the softkey functions.
z Using [Tab], activate channels or the main menu
z Using [Menu], open the menu line.
z Using <MODE>, change the measured parameter for the selected
channel (e. g. pH ><− mV).
z Using the functions keys on the meter such as Calibrate (<CAL>),
Store (<STO>), Print (<PRINT>), Display stored measurement data
(<RCL>), Manual stability control (<SC>) etc. you can execute fur-
ther functions.

Operating example: A channel is an indication on the display that corresponds to a physical

Highlighting a channel connection at the meter. The number of the channel is displayed with
the selected measured parameter, e.g. pH1, pH2.

Menu / chan-
nel, not high-

Channel,
highlighted

1 In the measured value display, highlight the main menu or a

channel with [Tab].
The highlighting moves on with each keypressing.

Mouse operation:

1 Select a channel or menu name with a mouse click.

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ProLab 3000 Operating principles

4.4.2 Navigation in menus

A menu item contains submenus, executes a function or opens a dialog

box.
The selection is made with the <S><T> and <W><X> keys or the
mouse.
The current selection is highlighted.
z Menus
Submenus are marked by an arrow to the right (X). Menus are
opened with <OK>.
z Functions
Functions are indicated by the name of the function. They are imme-
diately carried out by confirming with <OK>.
z Dialog boxes
Settings are marked by dots (...).
The relevant setting window is opened with <OK>.

Operating example: Menus are available for general settings and functions and for each
Navigation in the menu individual measured parameter.

1 In the measured value display, highlight the main menu or a

channel with [Tab].
A highlighted channel is marked by a frame.
2 Open the menu with the current selection with [Menu].
3 Highlight a menu item with <W><X> and <S><T>.
The highlighted menu item is displayed inverse.
4 Confirm the highlighted menu item with <OK>.

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Operating principles ProLab 3000

5 Highlight a menu item with <W><X> and <S><T>.

The highlighted menu item is displayed inverse.
6 Confirm the highlighted menu item with <OK>.
When a function is executed the main menu is closed and the
function executed, or a dialog box opens up.

Mouse operation:

1 Select a menu name with a mouse click.

The menu pops up.
2 Select a menu item and confirm with a mouse click.
When a function is executed the main menu is closed and the
function executed, or a dialog box opens up.

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ProLab 3000 Operating principles

4.4.3 Navigation in dialog boxes

Dialog boxes contain further subelements such as register cards,

selection lists and buttons. The elements are selected with the [Tab]
softkey. Within a list or register card, they are selected with the
<S><T> or <W><X> keys.

Operating example:
1 Using [Tab], highlight individual elements in a dialog box.
Navigation and settings
Highlighted elements such as register cards, option fields or
in dialog boxes
buttons are marked by a dotted frame.
Highlighted lists are displayed inverse.
2 If an element such as a register card or list that allows a further
selection is highlighted:
Highlight a different selection in element with <W><X> or
<S><T>.
3 If an element such as a button that executes a function is high-
lighted:
Use <OK> to execute the function.

Mouse operation:

1 Select an element with a mouse click.

The relevant function is executed.

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Operating principles ProLab 3000

4.4.4 Navigation in the file selection dialog box

The file selection dialog is opened if a file is to be created, stored,

selected or deleted.
All files are stored in the folder of the user. Each user can create or
delete subfolders in his/her folder.
The file dialog box contains further subelements such as
z symbol buttons,
z a list with files and subfolders,
z an input field for the file name and
z buttons.

The elements are selected with the [Tab] softkey. Within a list or regis-
ter card, they are selected with the <S><T> or <W><X> keys.
The functions of the symbol buttons available with the keypad using the
[Menu] softkey. A context menu opens up that contains all key func-
tions.

Note
Functions in file dialog boxes that are activated with symbol buttons
can be activated via the contest menu with [Menu] if you operate with-
out the mouse. Examples:

: move one folder up

: create new folder

: display/hide header

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ProLab 3000 Operating principles

Operating example:
1 Highlight an element in a file dialog box with [Tab]. Highlighted
Navigation and settings
elements such as buttons are marked by a dotted frame.
in file selection dialogs
Highlighted input fields are displayed inverse.
2 If an element such as the file list in the current folder allows a
further selection:
Highlight a different selection in element with <W><X> or
<S><T>.
3 If an element such as the input field for the file name allows an
entry:
Enter a name with <A...9>.
4 If an element such as a button that executes a function is high-
lighted:
Use <OK> to execute the function.

Mouse operation:

1 Select an element with a mouse click.

The assigned function is executed.
2 If an element such as the input field for the file name allows an
entry:
Enter a name with <A...9>.

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Operating principles ProLab 3000

46 ba75645e02 01/2010
ProLab 3000 Access to the meter

5 Access to the meter

5.1 Switch the meter on and off

Switching on
1 Switch the meter on with <On/Off>.
The self-test, meter designation, software version and subse-
quently the Login dialog box appear.

Switching off
1 Switch off the meter with <On/Off>.

5.2 Login as a user

After switching on, the Login dialog box pops up. Depending on the
adjusted type of access control, the dialog box contains:
z a list to select a user name and a field to enter a password or
z the prompt, Place the electronic key on the reader field.

In the delivery condition, access is possible with password.

Login with password

1 Select an item in the User name list.

2 Enter the correct password for the user name and confirm with
<OK>.
The login has taken place.

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Access to the meter ProLab 3000

Note
The default password for the administrator is "00001".
For your safety, change it as soon as possible (see page 49). No
password entry is required for anonymous access.

Login with electronic The Login dialog box displays the instruction:
key Place the electronic key on the reader field.

1 Place the electronic key on the read field and leave it there for
approx. 2 seconds until the key has been recognized.
The user name and key number is displayed. The login has
taken place.

Login with electronic The Login dialog box displays the instruction:
key and password Place the electronic key on the reader field.

1 Place the electronic key on the read field and leave it there for
approx. 2 seconds until the key has been recognized.
The user name and key number is displayed.
2 Enter the correct password and confirm with <OK>.
The login has taken place.

Note
Depending on user rights, individual functions and settings may be
grayed out and thus locked.
More information on user rights: See page 51.

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ProLab 3000 Access to the meter

5.3 Password for login

If the used of a password for login to the meter was set by the admin-
istrator, each user has to enter his or her password during the login.

The ProLab 3000 is delivered with the password "00001" for the user
name, Administrator . Change this password as soon as possible.

5.3.1 Changing the password

All users can change their own password.

A valid password consists of at least 5 characters.

1 Open the User / Change password... menu.

The Change password window opens.
The old password is in the Old password field.
2 Enter the new password in the New password field.
3 Enter the new password once again in the Confirm password
field.
4 Confirm the new password with [OK].
The password is changed.
The Change password window is closed.

5.3.2 Assigning a password

During the first login with the new user name the Change password
window appears. Here the password is set by the user. A valid pass-
word consists of at least 5 characters.

5.3.3 Forgotten the password?

If the access control requires the entry of a password, access to the

meter is not possible without the password.

Users The administrator can delete the password for user profiles (see
page 53).

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Access to the meter ProLab 3000

5.4 Lock
During operation (e.g. if the automatic storing function is active), the
activated lock prevents the inadvertent use of the meter with the name
of the registered user.
The lock can only can only be released with the currently registered
electronic key or the administrator key.

Note
The lock can only be activated in the measuring mode of operation.
With anonymous access, the Activate lock function is not available.

Activating the lock

1 Activate the lock in the System / Activate lock menu.
The meter is locked against inadvertent use.

Releasing the lock

1 Press any key.
The login window pops up.
2 Log in with the password, electronic key or electronic key and
password.
The lock is released.

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ProLab 3000 Access to the meter

5.5 Access control and user rights

You as the administrator define the type of access (assess control) to
the ProLab 3000. In addition, you define the rights to carry out functions
and measurements for the users (user rights).

When the meter is delivered, only the enclosed electronic administrator

key has an access authorization for the meter.
The administrator can set up access authorizations for electronic user
keys (see below).
Anonymous access without password and electronic key (user name,
Anonymous) is always possible. Measurement and calibration data is
identified by the user name, Anonymous.

After the login as administrator, the user management functions are

available in the Access control and Administration register cards of the
User / Administration... menu.

Access control In the Access control register card you define the general access set-
tings that will apply to all users.

User rights Each user is given user rights with the ProLab 3000. The user rights are
limited by the user type and configuration:

User type User rights

Administrator z Calibrate and measure
z Create file for manual storage
z Create user configuration
z Define type of access to the meter
z User administration
z Delete all files
Users
without configura- z Calibrate and measure
tion z Create file for manual storage
z Create user configuration
z Delete files in their own folder
with configuration z Calibrate and measure
(limited by settings in configuration file)

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Access to the meter ProLab 3000

User type User rights

Anonymous z Calibrate and measure
z Create file for manual storage
z Create user configuration
z Delete files in the folder, Anonymous

Note
Only one user can be the administrator. The administrator is adjusted
in the delivery condition. The administrator can only create users of
the type, user.

5.5.1 Access control

The administrator defines the type of access to the meter.

The setting applies to all users and the administrator.

Type of access Description

Access with password The meter can only be unlocked by enter-
ing the password for a certain user.
Access with electronic Access with electronic key only. No addi-
key tional password is required here.
Access with el. key and To be able to work with the meter, a pass-
password word has to be entered in addition to
access with the electronic key.

1 Open the User menu with Administration....

The User administration dialog box pops up.
The General register card is open.
2 Select the type of access and confirm with [OK].
The new setting is active when the ProLab 3000 is switched on
the next time.

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5.5.2 User management and assigning user rights

The ProLab 3000 has basic user management functions. The adminis-
trator can
z add new users
z delete users
z delete the password assigned to a user
z store a configuration file for users.
The configuration file contains measurement settings that cannot be
changed by the user. Besides, the administrator considerably
restricts the rights of the user.
The user cannot
– change or reset any defined measurement settings
– delete any files
– create or change any configuration
– backup any data
z hide the anonymous access or any user. If a user or anonymous
access is hidden, it is no longer available in the login dialog and can-
not be selected.

The administrator also defines the user rights for any new user (see
page 51).

The symbol buttons are used for user management with the mouse.
The relevant functions are available as a context menu with the [Menu]
softkey when operating with the meter keypad.

Symbol button Function (designation in context menu)

Add user
Delete user
Delete old password
Hide user/visible
Add configuration...

1 Open the User menu with Administration....

The User administration dialog box pops up.
2 Open the Administration register card.

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Access to the meter ProLab 3000

Adding a new user

1 Enter a new user name in the New user field.
or
Place an electronic user key on the reader field.
The key number and a user name already stored in the key are
displayed.
If necessary, enter a new user name in the New user field.
2 In the context menu, select the menu item, Add user.
The new user is included in the list of registered users.
On access with the electronic key, the user name is at the
same time stored in the electronic key.
3 Remove the electronic key from the reader field.

Assigning a
4 Highlight a user in the list of users.
configuration to a user
5 In the context menu, select the menu item, Add configuration....
The Open file dialog box pops up.
6 Select a configuration file and confirm with [OK].
The configuration file is permanently assigned to the user and
is also displayed in the list of users.

Erasing a registered
7 Highlight a user in the list of users.
user
8 In the context menu, select the menu item, Delete user.
A safety query appears. After confirming the safety query the
access authorization for the currently highlighted user is
deleted.

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Erasing the password

9 Highlight a user in the list of users.
for a registered user
10 In the context menu, select the menu item, Delete old pass-
word.
A safety query appears. After confirming the safety query the
password for the currently highlighted user is deleted.

Hiding / displaying a
11 Highlight a user in the list of users.
user
12 In the context menu, select the menu item, Hide user/visible.
If the user is hidden, the [Hidden] labeling is displayed in the list
of users.

Exiting the user

13 Confirm the settings with [OK].
management
The user settings are active.

5.6 Lost your electronic key?

Without an electronic key with access authorization, anonymous
access is possible only.

Electronic user key Keys for additional users can be obtained from SI Analytics GmbH.
The access authorization for new keys is set up by the administrator
(see page 53).

Electronic administrator If the electronic administrator key is lost, the access authorization for a
key new electronic administrator key can be set up in the factory only.

The addresses and telephone numbers of SI Analytics GmbH are given

on the cover of this operating manual.

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System functions ProLab 3000

6 System functions
System functions are all functions that are independent of the probe.

6.1 Overview: System settings

The following sensor-independent meter features can be adjusted in
the System menu and its submenus:

Menu item Setting Description

System

– Data output...

– USB printer ( )

– USB interface /Baud ( ) / 1200 ... 115200 Baud rate of the data interface

– Serial interface /Baud ( ) / 1200 ... 115200 Baud rate of the data interface

– Output format – ASCII Output format for data transmis-

– CSV sion
For details, see page 155

– Autom. printout... Printout interval Automatic output of the measure-

ment data to the interfaces at the
specified interval.

– Reset - Resets the system settings to the

default values.
For details, see page 65

– Measuring

– Autom. stability control ( ) You can activate or switch off the

automatic stability control func-
tion (see page 63).

– Temperature unit – Celsius All temperatures are displayed

– Fahrenheit with the selected unit.

– Connect Ref1/Ref2 ( ) Use reference electrode of the

other pH/ISE input

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Menu item Setting Description

System

– Signal On ( ) Switches on or off the acoustic

signal. The signal sounds e.g.
when a stable measured value
has been recognized (stability
control)

– Country and language...

– Country List with over 100 With this setting you can select
countries the country-specific notation for
the date, time and numbers.
The setting applies to all data
that is stored or output to an
interface.

– Language – Deutsch Select the menu language

– English
– Francais
– Espagñol

– Date and time...

– Date Setting the date

– Time Setting the time

– Color code... – SC stable Settings in the Color code group

– SC not stable field change the background
– Instruction display color for display indications with
certain states (stability criterion
– Information dis-
for stable measured values met /
play
not met) and for indications in the
info line.

– Activate lock ( ) After a user login with the elec-

tronic key, the key lock can be
activated here. The meter is
locked against operational
actions until the electronic key is
applied again.

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System functions ProLab 3000

6.2 Selecting the language

Menus can be displayed in different languages. The language is
selected in the System main menu.

1 Open the System / Country and language... menu.

The Country and language window opens.

2 Select a language in the Language list.

The active language is highlighted.
3 Confirm the selection with [OK] .
The language is changed.

6.3 Selecting the country

With this setting you can select the country-specific notation for the
date, time and numbers. This data is stored and output to an interface
in the country-specific notation.

1 Open the System / Country and language... menu.

The Country and language window opens.
2 Select a country in the Country list.
The active country is highlighted.
3 Confirm the selection with [OK] .
The country is changed.

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6.4 Setting the date and time

The data and time are displayed in the local format, according to the
Country setting (see above). The date and time are set in the menu,
System / Date and time....

1 Open the System / Date and time... menu.

The Date and time window opens.

2 Select the time zone in the Time zone field.

3 If necessary, mark the Automatic daylight saving time field.
4 Select the date.
5 Enter the time.
6 Confirm the entries with [OK] .
The date and time are taken over.

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System functions ProLab 3000

6.5 Selecting the channels for measured value display

In the Window / Channels... you can define which channels are visible
in the measured value display.
Depending on the number of active channels, one or two sensor data
is displayed.

Menu item Description

pH/U/ISE Probe 1 Display or hide the sensor data of the pH/U/ISE
1 channel.
pH/U/ISE Probe 2 Display or hide the sensor data of the pH/U/ISE
2 channel.

6.6 Interfaces for data download

The meter has three interfaces to which data can be downloaded:
z RS232: serial printer or PC
z USB-B (USB Device): PC
z USB-A (USB Host): USB printer

You can specify to which interfaces data will be downloaded at the

same time.

1 Open the System / Data output... menu.

The Data output window opens.

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2 Mark the option fields, USB printer, Serial interface and USB
interface.
The interfaces are selected for data download.
3 If data should be downloaded to the Serial interface or USB
interface interfaces:
Set the baud rate in the respective Baud list field.
The interface is ready for data download.

6.7 Temperature
The temperature affects the measurement results. You can select the
unit of the temperature display. The temperature is determined auto-
matically with an external temperature sensor or one that is integrated
in the sensor. It is also possible to enter temperature values manually.

6.7.1 Temperature unit

Measured temperature values can be displayed in the unit, °C (degrees

Celsius) or °F (degrees Fahrenheit).

1 Open the System / Measuring / Temperature unit menu and

select the unit, Celsius or Fahrenheit.
The selected temperature unit is active.

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System functions ProLab 3000

6.7.2 Temperature measurement

For reproducible measurements it is essential to determine the corre-

sponding temperature value.
The measuring system receives the temperature value from:
z a temperature sensor integrated in the sensor,
z a temperature sensor integrated in another sensor,
z an external temperature sensor NTC 30 or Pt 1000, or
z manual input of the temperature value.

On the display, the used temperature sensor is labeled with TP,

pH1 TP or pH2 TP.

Using an integrated Sensors with an integrated temperature sensor always measure the
temperature sensor measured parameter and temperature simultaneously. If the integrated
temperature sensor is recognized, the measured temperature value
and the TP status indicator appear on the display.

Using the temperature If no temperature sensor is available for a pH/ISE channel, you can
sensor of another measure the temperature with the integrated temperature sensor of
sensor another sensor.
To do so proceed as follows:

1 Immerse two electrodes in the measuring sample.

2 Highlight a channel in the measured value display.
3 In the sensor menu, select the Alternative TP menu item, e.g.
Measuring / Alternative TP.
The temperature measurement is active.
For checking purposes, the used temperature sensor is
indicated on the display.

Using an external If you want to measure the temperature using an external temperature
temperature sensor probe, proceed as follows:

1 Connect a temperature sensor to the measuring module.

2 Immerse the temperature sensor in the test sample.
The measured temperature value is shown on the display.

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6.8 Automatic stability control

The Autom. stability control function continuously checks the stability of
the measurement signal. The stability has a considerable impact on the
reproducibility of measured values.

You can activate or switch off the Autom. stability control function
(menu, Autom. stability controlSystem).

The Autom. stability control function is carried out:

z as soon as the measured value is outside the allowed stability range
z when you switch over between the measured parameters with
<MODE>.

Whether or not a measured value is stable can be taken from its color
labeling.

In the delivery condition, the SC stable / SC not stable conditions are

assigned to the following colors:

Color Meaning
red SC not stable
Stability criteria not met
green SC stable
Stability criteria met

SC stable

SC not stable

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System functions ProLab 3000

Note
The stability criteria that apply to a measured parameter are given in
the description of the measured parameter.

You can change the assignment of colors to conditions (see page 64).

6.9 Color assignment

For easy differentiation, the following conditions are highlighted by a
background color:
z Stability criterion met / not met
z Indications in the info line: Instruction / info display

You can adapt the colors yourself for these conditions.

1 Open the System / Color code... menu.

The Color code window pops up.

2 Open the Color palette window with [...].

3 Select a color.
4 Confirm the color with [OK].
The color is entered for the selected condition.
5 If necessary, change the background color for other conditions.
6 Confirm the color assignment with [OK].
The new color assignment is accepted and immediately
effective.

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6.10 Reset
You can reset (initialize) all sensor settings and sensor-independent
settings separately from each other.

6.10.1 Resetting the sensor settings

Note
The calibration data is reset to the default settings together with the
measuring parameters. Recalibrate after performing a reset.
The sensor settings are reset in the sensor menu.
In the measured value display, activate the relevant sensor window
[Tab] and open the menu with [Menu].

pH The following settings for pH measurements are reset to the default

settings with the Reset pHMeasuring function:

Setting Default settings

Calibration interval... 7d
Slope in mV/pH
Measured parameter pH
pH resolution High
Zero point 0 mV
Slope -59.16 mV/pH
Temperature, manual 25 °C

6.10.2 Resetting the system settings

The following system settings are reset to the default settings with the
System / Reset function:

Setting Default settings

Language English
Temperature unit Celsius
Signal On ✓

Baud rate (Baud) 4800

Output format ASCII

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System functions ProLab 3000

6.11 List of open windows

The Window menu item in the main menu provides an overview of the
open windows of the ProLab 3000. Open recorder windows and the
measured value display of the indicated sensors appear in the list of
open windows.

1 Open the Window menu.

2 Select a window from the list.
The window is activated and displayed in the foreground.

Note
Recorder windows that are open and displaced can be maximized and
restored with the Maximize recorder function.

Recorder window
1 Open the Window / Maximize recorder menu.
Recorder windows that are open and displaced are maximized.
The first recorder window is displayed.

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ProLab 3000 pH

7 pH
7.1 General information
Temperature For reproducible pH measurements, it is essential to measure the tem-
measurement perature of the test sample.
You have the following options to measure the temperature:
z Automatic temperature measurement with
– Temperature sensor (NTC 30 or Pt 1000) integrated in electrode
(if available).
– the integrated temperature sensor of another electrode that is
connected and immersed in the sample at the same time.
– an external temperature sensor (NTC 30 or Pt 1000).
z Manual determination and input of the temperature.

The display of the temperature indicates the active temperature mea-

suring mode:

Temperature Resolution of Mode

sensor the temp. dis-
play
yes 0.1 °C Automatic with temperature
sensor
- 1 °C Manual

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pH ProLab 3000

7.2 Measuring the pH value

7.2.1 Preparatory activities

Perform the following preparatory activities when you want to measure:

1 Adjust the temperature of the solutions and measure the cur-

rent temperature if the measurement is made without a temper-
ature sensor.
2 Connect a pH electrode to the meter.
3 Highlight the channel of the pH electrode in the measured
value display.
4 If necessary, select the pH display with <MODE>.
5 With manual temperature input:
– Call up the input window for the manual temperature input
with Measuring / Setting of man. temp....
– Measure the temperature of the test sample using a ther-
mometer.
– Enter the measured temperature value and confirm it with
[OK].
6 Calibrate the meter with the electrode.

Note
An incorrect calibration of pH electrodes leads to incorrect measured
values. Calibrate regularly. The meter has to be calibrated before
measuring.

Temperature sensor You can measure with or without a temperature sensor. The TP display
indicator appears when a temperature sensor is connected.

Note
If using a pH electrode without temperature sensor, you can also use
the temperature sensor of another connected electrode or conductiv-
ity measuring cell (see page 62). In this case, the indication from
which channel the temperature value is taken appears next to the tem-
perature value.

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ProLab 3000 pH

7.2.2 Measuring

1 Perform the preparatory activities (see page 67).

2 Immerse the pH electrode in the test sample.
3 If necessary, select the pH parameter with <MODE>.

Stability control The Stability control function checks the stability of the measurement
signal. The stability has a considerable impact on the reproducibility of
measured values. The background color is red until the measured
value is stable. When the measured value is stable, the background
color switches to green.
Regardless of the setting for Autom. stability control (see page 63) in
the System menu, you can start the Stability control function manually
at any time.

1 Highlight the channel of the electrode in the measured value

display.
2 If necessary, switch to the pH parameter with <MODE>.
3 Freeze the measured value with <SC>.
In the status line, [SC] is displayed.
4 With <OK> activate the Stability control function.
As long as the measured value does not meet the stability cri-
terion, the background color is red.
As soon as the measured value is stable, the background color
is green. The current measurement data is downloaded to the
interface. The measured values of all channels meeting the cri-
terion for stability control are marked by SC.

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pH ProLab 3000

Note
You can terminate prematurely the Stability control function with
<OK> manually at any time. If the Stability control function is termi-
nated prematurely, the current measurement data is not downloaded
to the interface.

5 Using <SC> or <MODE>, release the frozen measured value.

The [SC] status display disappears.

Criteria With identical measurement conditions, the following applies:

Measured param- Reproducibility Response time

eter
pH value Better than 0.01 > 15 seconds

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ProLab 3000 pH

7.2.3 Measurement settings

All settings for pH measurements are in the Measuring menu.

Menu item Setting Description

Measuring

– pH resolution High ( ) Resolution of the pH display:

Medium High = 0.001
Low Medium = 0.01
Low = 0.1

– Alternative TP ( ) ( ) = alternative temperature

sensor is used
( ) = no alternative tempera-
ture sensor is used

– Setting of man. temp... -35 ... +150 °C Entry of the manually deter-
mined temperature. For mea-
surements without
temperature sensor only.

– Reset pH Resets all sensor settings to

the delivery condition (see
page 65).

– Recorder... Start recorder (see page 139).

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pH ProLab 3000

7.3 Calibration
Why calibrate? pH electrodes age. This changes the zero point (asymmetry) and slope
of the pH electrode. As a result, an inexact measured value is dis-
played. Calibration determines the current values of the zero point and
slope of the electrode and stores them.
Thus, you should calibrate at regular intervals.

When do you have to z After connecting a non-ID electrode

calibrate?
z After the CalClock has expired

Buffer sets for You can use the buffer sets quoted in the table for an automatic calibra-
calibration tion. The pH values are valid for the specified temperature values. The
temperature dependence of the pH values is taken into account during
the calibration.

No. Buffer set * pH values at

1 DIN 1,679 25 °C
DIN buffers according to DIN 19266 4,006
and NIST Traceable Buffers 6,865
9,180
12,454
2 TEC 2,000 25 °C
4,010
7,000
10,011
3 Merck1 * (Technical buffers) 4,000 20°C
7,000
9,000
4 Merck2 * 1,000 20°C
6,000
8,000
13,000
5 Merck3 * 4,660 20°C
6,880
9,220
6 Merck4 * 2,000 20°C
4,000
7,000
10,000
7 Merck5 * 4,010 25 °C
7,000
10,000

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ProLab 3000 pH

No. Buffer set * pH values at

8 DIN 19267 * 1,090 25 °C
4,650
6,790
9,230
9 Mettler EU 1,679 25 °C
4,003
7,002
10,013
10 Mettler US * 1,995 25 °C
4,005
7,002
9,208
11 Fisher * 2,007 25 °C
4,002
7,004
10,002
12 Fluka * 4,006 25 °C
6,984
8,957
13 Radiometer * 1,678 25 °C
4,005
7,000
9,180
14 Baker * 4,006 25 °C
6,991
10,008
15 Metrohm * 3,996 25 °C
7,003
8,999
16 Beckmann * 4,005 25 °C
7,005
10,013
17 Hamilton DC * 4,005 25 °C
7,002
10,013
18 Precisa (Hamilton Duracal) * 3,996 25 °C
7,003
8,999

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pH ProLab 3000

No. Buffer set * pH values at

19 Reagecon TEC * 2,000 25 °C
4,010
7,000
10,000
20 Reagecon 20 * 2,000 20°C
4,000
7,000
10,000
13,000
21 Reagecon 25 * 2,000 25 °C
4,000
7,000
10,000
13,000
22 Riedel-de Haen * 2,000 20°C
4,000
7,000
10,000

* Brand names or trade names are trademarks of their respective owners

protected by law (see page 180).

Note
The buffers are selected in the Calibration sensor menu (see
page 71).

Calibration points Calibration can be performed using one to five buffer solutions in any
order (single-point to five-point calibration). The measuring instrument
determines the following values and calculates the calibration line as
follows:

Displayed calibration data

1-point z Zero point
z Slope = Nernst slope (-59.16 mV/pH at 25 °C)
2-point z Zero point
z Slope
3-point to z Zero point
5-point z Slope
The calibration line is calculated by linear regression.

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ProLab 3000 pH

Note
You can display the slope in the unit, mV/pH or % (see page 71).

Stability control The calibration procedure automatically activates the stability control
function. The current measurement with stability control can be termi-
nated at any time (accepting the current value).

Calibration record When finishing a calibration, the new calibration values are first dis-
played as an informative message. Then you can decide whether you
want to take over these values of the new calibration or whether you
want to continue measuring with the old calibration data. After accept-
ing the new calibration values the calibration record is displayed.

Display of calibration You can display the data of the last calibration (see page 134). Subse-
data and download to quently, you can transmit the displayed calibration data to the interface,
interface e. g. to a printer or PC, with the <PRINT> key.

Note
The calibration record is automatically transmitted to the interface
after calibrating.

Sample record:

Print date26.04.07 16 was selected:13

ProLab 3000 (06249876)
Current user: 1234567890
CALIBRATION pH
Calibration date20.04.07 10:14:03
A 161 1M-BNC-ID (A062498765)
User: Administrator

Calibration interval 7 days

AutoCal DIN 2-point TAuto SC
Buffer 1 6.86
Buffer 2 9.18
Voltage 1 7.2 mV 26.3 °C
Voltage 2 -171 mV 26.3 °C
Slope -59.2 mV/pH
Zero point -0.5 mV
Sensor +++

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pH ProLab 3000

Calibration evaluation After calibrating, the meter automatically evaluates the calibration. The
zero point and slope are evaluated separately. The worse evaluation of
both is taken into account. The evaluation appears on the display and
in the calibration record.

Display Calibration Zero point Slope

record [mV] [mV/pH]
+++ - 18 ... + 18 -60.5 ... -57.5

++ - 22 ... + 22 -57.5 ... -56

+ - 26 ... + 26 -61 ... -60.5

or
-56 ... -55
- - 30 ... + 30 -62 ... -61
or
-55 ... -50
Clean the electrode according to the
sensor operating manual
< - 30 or ... -62 or
Error Error
> 30 ... -50
Eliminate the error according to chapter
15 WHAT TO DO IF... (page 165)

Preparatory activities Perform the following preparatory activities when you want to calibrate:

1 Connect a pH electrode to the meter.

2 Highlight the channel of the electrode in the measured value
display.
3 If necessary, select the pH display with <MODE>.
4 Keep the buffer solutions ready. Adjust the temperature of the
buffer solutions, or measure the current temperature, if you
measure without a temperature sensor.

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7.3.1 Carrying out a calibration procedure

(example: AutoCal DIN)

Make sure the Calibration / pH calibration type / Buffer sets setting is

selected correctly and the AutoCal calibration type is marked (see
page 71).

Use any one to five buffer solutions of the selected buffer set.

The DIN calibration is described below. With other buffer sets, other
nominal buffer values are displayed. Apart from that, the procedure is
identical.

1 Perform the preparatory activities (see page 76).

2 Start the calibration with <CAL>.
The pHX calibration window opens.

3 If necessary, check whether the displayed buffer set agrees

with the used buffer set.
4 When measuring without temperature sensor:
z Measure the temperature of the standard solution using a
thermometer.
z Open the setting window for the temperature value with
[Man. temp.].
z Enter the temperature and confirm with [OK].
5 Thoroughly rinse the electrode with distilled water.
6 Immerse the electrode in buffer solution 1.
7 Start the measurement with [Continue].
The measured value is checked for stability (Stability control).

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pH ProLab 3000

1 Recognized nominal buffer value (referring to 25 °C)

2 Current electrode voltage
3 Current temperature value
4 Selected buffer set

8 Wait for the measurement with stability control to end.

or
Terminate the stability control with [OK].
The pHX calibration window for the according to buffer solution
pops up and the Finish with 1-point calibration infotext is dis-
played.

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ProLab 3000 pH

9 Using [OK], confirm the Finish with 1-point calibration infotext

and take over the new calibration values.
The calibration record is displayed and output to the interface.
The calibration procedure is finished.
or
Continue the calibration with [Continue].
or
Cancel the calibration procedure without taking over the cali-
bration values with [Cancel], or <ESC>.

Note
For single-point calibration, the meter uses the Nernst slope (-
59.16 mV/pH at 25 °C) and determines the zero point of the electrode.

Continuing with two-

10 When measuring without temperature sensor:
point calibration
z Measure the temperature of the standard solution using a
(Buffer: DIN)
thermometer.
z Open the setting window for the temperature value with
[Man. temp.].
z Enter the temperature and confirm with [OK].
11 Thoroughly rinse the electrode with distilled water.
12 Immerse the electrode in buffer solution 2.
13 Start the measurement with [Continue].
The measured value is checked for stability (Stability control).
14 Wait for the measurement with stability control to end.
or
Terminate the stability control with [OK].
The pHX calibration window for the according to buffer solution
pops up and the Finish with 2-point calibration infotext is dis-
played.
15 Using [OK], confirm the Finish with 2-point calibration infotext
and take over the new calibration values.
The calibration record is displayed and output to the interface.
The calibration procedure is finished.
or
Continue the calibration with [Continue].
or
Cancel the calibration procedure without taking over the cali-
bration values with [Cancel], or <ESC>.

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pH ProLab 3000

Note
A calibration line is determined for the two-point calibration.

Continuing with three-

16 When measuring without temperature sensor:
point to five-point
z Measure the temperature of the standard solution using a
calibration
thermometer.
(Buffer ) DIN)
z Open the setting window for the temperature value with
[Man. temp.].
z Enter the temperature and confirm with [OK].
17 Thoroughly rinse the electrode with distilled water.
18 Immerse the electrode in buffer solution 3.
19 Start measurement of the next buffer with [Continue].
The measured value is checked for stability (Stability control).
20 Wait for the measurement with stability control to end.
or
Terminate the stability control with [OK].
The pHX calibration window for the according to buffer solution
pops up and the Finish with 3-point calibration infotext is dis-
played.
21 Using [OK], confirm the Finish with 3-point calibration infotext
and take over the new calibration values.
The calibration record is displayed and output to the interface.
The calibration procedure is finished.
or
Continue the calibration with [Continue].
or
Cancel the calibration procedure without taking over the cali-
bration values with [Cancel], or <ESC>.

Note
The calibration is automatically finished after all buffers of a buffer set
were used.

Note
The calibration line is determined by linear regression.

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7.3.2 Carrying out a calibration procedure (example: VariCal)

Make sure the Calibration / pH calibration type / VariCal calibration type

is marked (see page 71).

Single-point calibration Use any buffer solution for this rapid method.
The calibration will be the more exact the nearer the pH value of the
buffer solution is to that of the test sample.

Two-point calibration Use two buffer solutions for this procedure:

z first buffer solution: pH 7.000 ± 0.500
z any other buffer solution

To carry out the calibration procedure, the pH calibration type VariCal

must be set in the sensor menu (see page 85).

1 Perform the preparatory activities (see page 76).

2 Start the calibration with <CAL>.
The pHX calibration window opens.

3 When measuring without temperature sensor:

z Measure the temperature of the standard solution using a
thermometer.
z Open the setting window for the temperature value with
[Man. temp.].
z Enter the temperature and confirm with [OK].
4 Thoroughly rinse the electrode with distilled water.
5 Immerse the electrode in buffer solution 1 (pH 7.000 ± 0.500).
6 Start the measurement with [Continue].
The measured value is checked for stability (Stability control).

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pH ProLab 3000

1 Recognized nominal buffer value (referring to 25 °C)

2 Current electrode voltage
3 Current temperature value
4 Selected buffer set

7 Wait for the measurement with stability control to end.

or
Terminate the stability control with [OK].
The pHX calibration window for the according to buffer solution
pops up and the Finish with 1-point calibration infotext is dis-
played.

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ProLab 3000 pH

8 Using [OK], confirm the Finish with 1-point calibration infotext

and take over the new calibration values.
The calibration record is displayed and output to the interface.
The calibration procedure is finished.
or
Continue the calibration with [Continue].
or
Cancel the calibration procedure without taking over the cali-
bration values with [Cancel], or <ESC>.

Note
For single-point calibration, the meter uses the Nernst slope (-
59.16 mV/pH at 25 °C) and determines the zero point of the electrode.

Continuing with two-

9 When measuring without temperature sensor:
point calibration
z Measure the temperature of the standard solution using a
thermometer.
z Open the setting window for the temperature value with
[Man. temp.].
z Enter the temperature and confirm with [OK].
10 Thoroughly rinse the electrode with distilled water.
11 Immerse the electrode in buffer solution 2.
12 Start the measurement with [Continue].
The measured value is checked for stability (Stability control).
13 Wait for the measurement with stability control to end.
or
Terminate the stability control with [OK].
The pHX calibration window for the according to buffer solution
pops up and the Finish with 2-point calibration infotext is dis-
played.
14 Using [OK], confirm the Finish with 2-point calibration infotext
and take over the new calibration values.
The calibration record is displayed and output to the interface.
The calibration procedure is finished.
or
Continue the calibration with [Continue].
or
Cancel the calibration procedure without taking over the cali-
bration values with [Cancel], or <ESC>.

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pH ProLab 3000

Note
A calibration line is determined for the two-point calibration.

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7.3.3 Calibration settings and calibration data

In the Calibration menu, you can

z set the calibration interval,
z define the calibration type and
z view the calibration data.

Menu item Setting Description

Calibration

– pH calibration type

– AutoCal ( ) Calibration with commercial buffer sets.

The buffer set is selected with the next
menu item (Buffer sets)

– Buffer sets DIN ( ) Buffer sets to be used for pH calibration.

TEC For details, see page 72.
...

– VariCal ( ) Calibration with any buffer solutions

– Slope in mV/pH ( ) Unit of the slope.

% The % display refers to the Nernst slope of
-59.16 mV/pH ([determined slope/Nernst
slope ] x 100).

– Zero point in mV ( ) Unit for the zero point.

pH

– Calibration interval... 1 ... 999 Calibration interval... for the pH electrode

(in days).
The meter reminds you to calibrate regu-
larly by the red frame around the CalClock
in the measuring window.

– Calibration history... Displays the calibration history of the last

calibrations.

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pH ProLab 3000

7.3.4 Calibration interval

The calibration interval and calibration evaluation are indicated on the

display as the CalClock.

CalClock

The remaining time of the calibration interval is indicated by the seg-

mented ring around the calibration evaluation. This segmented ring
reminds you to calibrate regularly.
The CalClock appears on a red square after the specified calibration
interval has expired. It is still possible to measure.

Note
To ensure the high measuring accuracy of the measuring system, cal-
ibrate after the calibration interval has expired.

Setting the calibration The calibration interval is set to 7 days in the factory.
interval You can change the interval (1 ... 999 days):

1 Highlight the channel of the electrode in the measured value

display.
2 If necessary, select the pH display with <MODE>.
3 Open the input window for the calibration intervals with Calibra-
tion / Calibration interval....
4 Enter the calibration interval with <0...9>.
5 Confirm the setting with <OK>.

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8 ORP voltage
8.1 General information
You can measure the following variables:
z ORP [mV]
z Relative ORP [mV]

Temperature The temperature value is measured and documented with all measure-
measurement ments.
You have the following options to measure the temperature:
z Automatic temperature measurement with
– Temperature sensor (NTC 30 or Pt 1000) integrated in electrode
(if available).
– the integrated temperature sensor of another electrode that is
connected and immersed in the sample at the same time.
– an external temperature sensor (NTC 30 or Pt 1000).
z Manual determination and input of the temperature.

The display of the temperature indicates the active temperature mea-

suring mode:

Temperature Resolution of Mode

sensor the temp. dis-
play
yes 0.1 °C Automatic with temperature
sensor
- 1 °C Manual

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ORP voltage ProLab 3000

8.2 Measuring the ORP

8.2.1 Preparatory activities

Perform the following preparatory activities when you want to measure:

1 Adjust the temperature of the solutions and measure the cur-

rent temperature if the measurement is made without a temper-
ature sensor.
2 Connect the ORP electrode to the meter.
3 Highlight the channel of the electrode in the measured value
display.
4 If necessary, select the U or dU display with <MODE>.
5 Adjust the temperature of the solutions and measure the cur-
rent temperature if the measurement is made without a temper-
ature sensor.
6 With manual temperature input:
Call up the manual input of the temperature value with
Measuring / Setting of man. temp....
7 Enter the measured temperature value and confirm it with [OK].
8 Check the meter with the electrode.

Note
ORP electrodes are not calibrated. However, you can check ORP
electrodes using a measuring solution.

Temperature sensor You can measure with or without a temperature sensor. The TP display
indicator appears when a temperature sensor is connected.

Note
If using an ORP electrode without temperature sensor, you can also
use the temperature sensor of another electrode (see page 62). In this
case, the indication from which channel the temperature value is
taken appears next to the temperature value.

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8.2.2 Measuring

Note
ORP electrodes are not calibrated. However, you can check ORP
electrodes using a test solution.

1 Perform the preparatory activities (see page 67).

2 Highlight the channel of the electrode in the measured value
display.
3 If necessary, select the U display with <MODE>.
4 Immerse the ORP electrode in the test sample.

Stability control TheStability control function checks the stability of the measurement
signal. The stability has a considerable impact on the reproducibility of
measured values. The background color is red until the measured
value is stable.
Regardless of the setting for Autom. stability control (see page 63) in
the System menu, you can start the Stability control function manually
at any time.

1 Highlight the channel of the ORP electrode.

2 If necessary, select the U or dU parameter with <MODE>.
3 Freeze the measured value with <SC>.
The display switches to the display of the selected channel as
necessary.
In the status line, [SC] is displayed.

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ORP voltage ProLab 3000

4 With <OK> activate the Stability control function.

The background color is red as long as the measured value
does not meet the stability criterion.
As soon as the measured value is stable, the background color
is green. The current measurement data is downloaded to the
interface. The measured values of all channels meeting the cri-
terion for stability control are marked by SC.

Note
When you measure the electromotive force, please note that it may
take some time until the measured value is stable.

Note
You can terminate prematurely the Stability control function with
<OK> manually at any time. If the Stability control function is termi-
nated prematurely, the current measurement data is not downloaded
to the interface.

5 Using <SC> or <MODE>, release the frozen measured value.

The [SC] status display disappears.

Criteria With identical measurement conditions, the following applies:

Measured param- Reproducibility Response time

eter
ORP voltage better than 0.3 mV > 15 seconds

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8.2.3 Measuring the relative ORP

Note
The relative ORP can be determined with pH and ORP electrodes.

To measure the difference of the ORPs of two solutions it is best to use

two ORP electrodes. Define one of the ORP electrodes as the refer-
ence in the menu. The difference of the two voltages is displayed.

1 Perform the preparatory activities (see page 67).

2 Prepare the reference solution for the determination of the ref-
erence point.
3 Immerse one electrode in the reference solution.
Immerse one electrode in the test sample.
4 Highlight the channel of the electrode that is in the test sample
in the measured value display.
5 If necessary, select the U display with <MODE>.
6 Display the difference voltage with Measuring / Referenz / an-
derer U-Kanal.

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ORP voltage ProLab 3000

As an alternative you can also determine the difference of the ORPs of

two solutions with one ORP electrode only. To do so, first define the
ORP of one solution as the zero point.

1 Perform the preparatory activities (see page 67).

2 Prepare the reference solution for the determination of the ref-
erence point.
3 Immerse the electrode in the reference solution.
4 Highlight the channel of the electrode in the measured value
display.
5 If necessary, select the U display with <MODE>.
6 Display the voltage of the current zero point with <CAL> or
Measuring / Referenz / Determine reference....
7 Measure the reference solution with <OK>.
The measured voltage is defined as the zero point.
or
Terminate the display of the zero point with <ESC>.
8 Rinse the electrode and immerse it into the test sample.

Stability control TheStability control function checks the stability of the measurement
signal. The stability has a considerable impact on the reproducibility of
measured values. The background color is red until the measured
value is stable.
Regardless of the setting for Autom. stability control (see page 63) in
the System menu, you can start the Stability control function manually
at any time.

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1 In the measured value display, mark the channel of the ORP or

pH electrode.
2 If necessary, select the U or dU parameter with <MODE>.
3 Freeze the measured value with <SC>.
The display switches to the display of the selected channel as
necessary.
In the status line, [SC] is displayed.
4 With <OK> activate the Stability control function.
The background color is red as long as the measured value
does not meet the stability criterion.
As soon as the measured value is stable, the background color
is green. The current measurement data is downloaded to the
interface. The measured values of all channels meeting the cri-
terion for stability control are marked by SC.

Note
When you measure the electromotive force, please note that it may
take some time until the measured value is stable.

Note
You can terminate prematurely the Stability control function with
<OK> manually at any time. If the Stability control function is termi-
nated prematurely, the current measurement data is not downloaded
to the interface.

5 Using <SC> or <MODE>, release the frozen measured value.

The [SC] status display disappears.

Criteria With identical measurement conditions, the following applies:

Measured param- Reproducibility Response time

eter
ORP voltage better than 0.3 mV > 15 seconds

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ORP voltage ProLab 3000

8.2.4 Measurement settings

The following settings are possible for ORP measurements in the Mea-
suring menu:

Menu item Setting Description

Measuring

– High resolution ( ) Resolution of the mV display:

( ) = 0.1 mV
( ) = 1 mV

– Alternative TP ( ) ( ) = Temperature sensor of

another channel is used

– Setting of man. temp... -35 ... +150 °C Entry of the manually determined
temperature value. For measure-
ments without temperature sensor
only.

– Determine reference... Only if the relative ORP (dU) is

selected as the measured param-
eter

– Recorder... Start recorder (see page 139).

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9 Ion concentration
9.1 General information

Note
Incorrect calibration of ion sensitive electrodes will result in incorrect
measured values. Calibrate regularly before measuring.

Temperature The temperature value is measured and documented with all measure-
measurement with ion ments.
selective measurements You have the following options to measure the temperature:
z Automatic temperature measurement with
– the temperature sensor integrated in the electrode (NTC 30 or
Pt 1000) .
– the integrated temperature sensor of another electrode that is
connected and immersed in the sample at the same time.
– an external temperature sensor (NTC 30 or Pt 1000).
z Manual determination and input of the temperature.

The display of the temperature indicates the active temperature mea-

suring mode:

Temperature Resolution of Mode

sensor the temp. dis-
play
yes 0.1 °C Automatic with temperature
sensor
- 1 °C Manual

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Ion concentration ProLab 3000

9.2 Measuring the ion concentration

9.2.1 Preparatory activities

Perform the following preparatory activities when you want to measure:

1 Connect an ion sensitive electrode to the meter.

2 Highlight the channel of the electrode in the measured value
display.
3 If necessary, select the ISE display with <MODE>.
4 Open the Measuring / ISE settings / Ion type menu and select
the ion type to be measured.
5 With manual temperature input:
– Measure the temperature of the test sample using a ther-
mometer.
– Open the Measuring / Setting of man. temp... menu.
The Manual temperature window opens.
– Enter the measured temperature value and confirm it with
[OK].
6 Calibrate the meter with the electrode (see page 101).
or
For measurement with a method, start a method.
The calibration is included in the course of the measuring
method (see page 111 ff.).

Note
While no valid calibration is available, e.g. in the delivery condition,
Error appears in the measured value display.

Temperature sensor You can measure with or without a temperature sensor. The TP display
indicator appears when a temperature sensor is connected.

Note
If using an ion sensitive electrode without temperature sensor, you
can also use the temperature sensor of another electrode (see
page 62). In this case, the indication from which channel the temper-
ature value is taken appears next to the temperature value.

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9.2.2 Measuring

1 Perform the preparatory activities according to page 95.

2 Immerse the electrode in the test sample.

Temperature for For precise ion selective measurements, the temperature difference
calibrating and between measurement and calibration should not be greater that 2 K.
measuring Therefore, adjust the temperature of the standard and measuring solu-
tions accordingly. If the temperature difference is greater the [TempErr]
warning appears in the measured value display.

Stability control The Stability control function continuously checks the stability of the
measurement signal. The stability has a considerable impact on the
reproducibility of measured values. The background color is red until
the measured value is stable.

Regardless of the setting for automatic Autom. stability control (see

page 63) in the System menu, you can start the Stability control func-
tion manually at any time.

1 Highlight the channel of the electrode in the measured value

display.
2 If necessary, switch to the ISE display with <MODE>.
3 Freeze the measured value with <SC>.
In the status line, [SC] is displayed.

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Ion concentration ProLab 3000

4 With <OK> activate the Stability control function.

While the measured value does not meet the stability criterion,
the background color is red.
As soon as a stable measured value is recognized, the current
measurement data is downloaded to the interface. The mea-
sured values of all channels meeting the criterion for stability
control are marked by SC.

Note
You can terminate prematurely the Stability control function with
<OK> manually at any time. If the Stability control function is termi-
nated prematurely, the current measurement data is not downloaded
to the interface.

5 Using <SC> or <MODE>, release the frozen measured value.

The [SC] status display disappears.

Criteria The criteria of the stability control affect the reproducibility of the mea-
sured values.
If possible, always use the highest reproducibility criteria.
If the stability criteria cannot be achieved with your test sample and
electrode, you can switch over to lower reproducibility criteria. The fol-
lowing criteria can be adjusted:
z High: highest reproducibility
z Medium: medium reproducibility
z Low:lowest reproducibility

Note
Increasing reproducibility also causes the response time to increase
until a measured value is evaluated as stable.

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9.2.3 Measurement settings

All settings for ion selective measurements are done in the Measuring
menu.

Menu item Setting Description

Measuring

– ISE settings

– Units – mg/l Selection with which unit the mea-

– mol/l surement result and calibration
– mg/kg standards should be displayed.
– ppm
– %

– Ion type Ag, Br, Ca, Cd, Cl, CN, CO2, Selection of the ion type to be
Cu, F, I, K, Na, NH3, NH4, measured
NO3, Pb, S, ION
With the ION setting it is possible
to measure ions that are not men-
tioned in the list. Depending on
the selected unit, (see ISE set-
tings/Units) a further entry may be
necessary (molar mass of the ion
or density of the solution).

– Blank value correction

– Reference measure-
ment

– Input reference
value...

– Stability control – High ( ) Selection of criteria for the stabil-

– Medium ity control (see page 98).
– Low

– ISE methods – Standard addition... Selection of the available measur-

– Double standard addition ing methods.
– Standard subtraction...
– Sample addition...
– Sample subtraction...
– Blank value addition...

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Ion concentration ProLab 3000

Menu item Setting Description

Measuring

– Alternative TP ( ) ( ) : alternative temperature sen-

sor is used
( ) : no alternative temperature
sensor is used

– Setting of man. temp... -35 ... +150 °C Entry of the manually determined
temperature. For measurements
without temperature sensor only.

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ProLab 3000 Ion concentration

9.3 Calibration
Why calibrate? Ion-selective electrodes age and are temperature-dependent. This
changes the slope. As a result, an inexact measured value is displayed.
Calibration determines the current value of the slope of the electrode
and stores it in the instrument.

Thus, you should calibrate before each measurement and at regular

intervals.

When to calibrate? z Before any measurement if possible

z After connecting another ion selective (combination) electrode

Standard solutions Use two to nine different standard solutions. The standard solutions
have to be selected in either increasing or decreasing order.

Standard solution (Std 1 - Values

9)
Unit [mg/l] 0.001 ... 500000
Unit [mol/l] 0.010 ... 5000 µmol/l
10.00 ... 5000 mmol/l
Unit [mg/kg] 0.001 ... 500000
Unit [ppm] 0.001 ... 500000
Unit [%] 0.0001 ... 50000

In addition to the list with defined values for the standard solutions, you
can enter any concentration for a standard.

Note
The measurement precision is also dependent on the selected stan-
dard solutions. Therefore, the selected standard solutions should
cover the expected value range of the subsequent concentration mea-
surement.

Temperature for For precise ion selective measurements, the temperature difference
calibrating and between measurement and calibration should not be greater that 2 K.
measuring Therefore, adjust the temperature of the standard and measuring solu-
tions accordingly. If the temperature difference is greater the [TempErr]
warning appears in the measured value display.

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Ion concentration ProLab 3000

ISE Cal This is the conventional two-point to nine-point calibration proce-

dure that uses 2 to 9 freely selectable standard solutions. The concen-
tration expected in the measurement determines the concentration of
the calibration standards.

Stability control During calibration, the stability control is automatically activated.

The current measurement with stability control can be terminated at
any time (accepting the current value).

Calibration record The new calibration values are displayed when a calibration procedure
is finished. Then you can decide whether you want to take over these
values of the new calibration or whether you want to continue measur-
ing with the old calibration data. After accepting the new calibration val-
ues the calibration record is displayed.

Display of calibration You can display the data of the last calibration (see page 136). Subse-
data and download to quently, you can transmit the displayed calibration data to the interface,
interface e. g. to a printer or PC, with the <PRINT> key.

Note
The calibration record is automatically transmitted to the interface
after calibrating.

Sample record:

Print date 26.04.07 16:13

ProLab 3000 (06249876)
Current user: 1234567890
CALIBRATIONISE
Calibration date 20.04.07 10:14:03
User: Administrator

Standard 1: 0.010 mg/l

Standard 2: 0.020 mg/l
Voltage 1: 0.0 mV 24.0 °C
Voltage 2: 9.0 mV 24.0 °C
Ion type: Ag
Slope: 29.9 mV
Sensor +++

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ProLab 3000 Ion concentration

Calibration evaluation After calibrating, the meter automatically evaluates the calibration.

Display Calibration Magnitude of the slope [mV]

record
Ions with Ions with
valency 1 valency 2
+++ 50.0 ... 70.0 25.0 ... 35.0

< 50 or > 70 < 25 or > 35

Error Error
Eliminate the error according
to chapter 15 WHAT TO DO IF...
(page 165)

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Ion concentration ProLab 3000

9.3.1 Carrying out calibration

Use two to nine standard solutions in ascending or descending order.

Note
When calibrating within the framework of a method measurement, it is
always a two-point calibration.

Preparatory activities Perform the following preparatory activities when you want to calibrate:

1 Connect an ion sensitive electrode to the meter.

2 Highlight the channel of the electrode in the measured value
display.
3 If necessary, select the ISE display with <MODE>.
4 If necessary, open the Measuring / ISE settings / Ion type menu
and select the ion type to be measured.
5 Keep the standard solutions ready.
6 If necessary, measure the temperature of the standard solu-
tions with a thermometer.
7 If necessary, change the unit of the measurement result and
calibration standards in the menu, Measuring/Units.

Calibration
1 Perform the preparatory activities (see page 104).
2 Start the calibration with <CAL>.
The ISEX calibration window opens.

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ProLab 3000 Ion concentration

3 When measuring without temperature sensor:

z Measure the temperature of the standard solution using a
thermometer.
z Open the setting window for the temperature value with
[Man. temp.].
z Enter the temperature and confirm with [OK].
4 Thoroughly rinse the electrode with distilled water.
5 Immerse the electrode in standard solution 1.
6 Open the ISE standard input window with [Continue].

7 Select a value from the Concentration list.

or
Enter a value with <0...9>.
or
Cancel the calibration with [Cancel].

Note
To enter a concentration in exponential representation such as
1.00 E2 mg/l:
Enter the mantissa with <0...9> (here: 1.00).
Enter the exponential sign (E) with <- .><- .>.
Enter the exponent with <0...9> (here: 2).

8 Start the measurement with [OK].

The measured value is checked for stability (Stability control).

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Ion concentration ProLab 3000

9 Wait for the measurement with stability control to end.

or
Take over the calibration value with [OK].
The ISE standard input window for the entry of the concentra-
tion of the second standard solution pops up.

Continuing with two-

10 Select a value from the ISE-Std list.
point calibration
or
Enter a value and, if necessary, an exponent with <0...9>.
or
Cancel the calibration with [Cancel].

Note
To enter a concentration in exponential representation such as
1.00 E2 mg/l:
Enter the mantissa with <0...9> (here: 1.00).
Enter the exponential sign (E) with <- .><- .>.
Enter the exponent with <0...9> (here: 2).

11 When measuring without temperature sensor:

z Measure the temperature of the standard solution using a
thermometer.
z Open the setting window for the temperature value with
[Man. temp.].
z Enter the temperature and confirm with [OK].
12 Thoroughly rinse the electrode with distilled water.
13 Immerse the electrode in standard solution 2.
14 Start the measurement with [OK].
The measured value is checked for stability (Stability control).
15 Wait for the measurement with stability control to end.
or
Take over the calibration value with [OK].
The ISEX calibration window for the next standard solution
pops up and the Finish with 2-point calibration infotext is dis-
played.

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ProLab 3000 Ion concentration

16 Using [OK], confirm the Finish with 2-point calibration infotext

and take over the new calibration values.
The calibration record is displayed and output to the interface.
The calibration procedure is finished.
or
Continue the calibration with [Continue].
or
Cancel the calibration procedure without taking over the cali-
bration values with <ESC>.

Note
A calibration line is determined for the two-point calibration.

Continuing with three- Repeat the steps 9 to 16 in the same way with the third and further stan-
point to nine-point dard solutions as necessary. The new calibration values are displayed
calibration after the last calibration step is finished.

Note
Based on the calibration data, the calibration line is determined in sec-
tions. A straight line of the calibration is determined according to the
Nernst equation between the highest and second highest calibration
standard.
Between the lowest and second lowest calibration standard, a calibra-
tion line is determined according to the Nernst equation modified by
Nikolski. The Nikolski equation reproduces the real course of the char-
acteristic curve of the electrode for the low concentration range.

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Ion concentration ProLab 3000

9.3.2 Calibration data

In the Calibration menu, you can view the calibration data of ISE cali-
brations.

Menu item Description

Calibration

– Calibration record... Displays the calibration record of the last

calibration.

– Calibration history... Displays the calibration history of the last

calibrations.

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9.4 Blank value correction

If the ion concentration in the sample is so small that it is not in the lin-
ear range of the electrode, you can increase the ion concentration in
the linear range with the blank value correction by the addition of a
blank value solution.

The displayed measured value is the difference between the actual

measured value and the determined blank value.

Calibration
1 In the Measuring / ISE Setup menu, select and confirm the
menu item, Blank value correction.
The Blank value correction function is active (✔).
2 Carry out a 2 to 5-point calibration according to the user guide
(see page 104).
As soon as a stable value is achieved for the standard calibra-
tion solution, the slope (mV) and evaluation (Error, +++) of the
electrode after calibration is displayed.
The infotext, Accept calibration and terminate is displayed.
3 Using [OK], confirm the infotext Accept calibration and termi-
nate.
The calibration record is displayed and output to the interface.
The calibration procedure is finished.
4 Close the calibration record with [OK].
The prompt for the measurement of the blank value appears.

Measuring
5 Thoroughly rinse the electrode with deionized water.
6 Immerse the electrode in the blank value solution.
7 Start the measurement with [Continue].
The measured value is checked for stability (Stability control).
8 Wait for the measurement with stability control to end.
or
Take over the measured value with [OK].
The calibration record is displayed and output to the interface.
The calibration procedure is finished.
9 Close the calibration record with [OK].

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9.5 Reference measurement

A reference measurement enables you to assign any concentration
value to the calibration curve of your electrode for a specific ion con-
centration (reference concentration).
Prerequisite: A valid calibration must be available.

Determining the
1 Only if no valid calibration is available
reference value and
(no sensor symbol on the measured value display):
activating reference
Perform a calibration (see page 101).
measurement
2 Prepare the solution with reference concentration.
3 Open the Measuring / Input reference value...ISE settings
menu.
The window for the entry of the reference value pops up.
4 Enter the reference value and confirm with [OK].
The [ISERef] symbol is displayed in the status line.
The measurement with reference value is active.

Switching off the

1 In the Measuring / ISE settings menu, select and confirm the
reference measurement
menu item, Reference measurement.
The Reference measurement function is switched off.
The [ISERef] symbol disappears from the status line.

Activating reference If a reference value was determined but the reference measurement
measurement with was switched off, the reference value does not have to be determined
existing reference value again if you want to measure with the reference. The meter has stored
the last reference value with the respective concentration.

1 In the Measuring / ISE settings menu, select and confirm the

menu item, Reference measurement.
The Reference measurement function is activated.
The [ISERef] symbol is displayed in the status line.

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9.6 Measuring with increment procedure (methods)

9.6.1 Selecting the measuring method

The following methods are supported:

z Standard addition
z Double standard addition
z Standard subtraction
z Sample addition
z Standard subtraction
z Blank value addition (Standard addition with blank value correction)

1 Perform the preparatory activities (see page 101).

2 Highlight the channel of the electrode in the measured value
display.
3 If necessary, select the ISE display with <MODE>.
4 Thoroughly rinse the electrode with distilled water.
5 Adjust the temperature of the calibration standard solutions.
6 Select a unit in the menu, Measuring / ISE settings / Units .
7 Select a method in the menu, Measuring / ISE methods .
The selected method is started (see page 112 ff).

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9.6.2 Standard addition

In the Standard addition procedure, a known amount of standard solu-

tion is added to the sample.
The ion concentration in the sample is calculated from the change in
potential.

1 Check the currently displayed ion.

If the ion to be measured is displayed incorrectly:
Select the ion type in the menu, Measuring / ISE methods / Ion
type.
2 In the Measuring / ISE methods menu, select the method,
Standard addition.
The ISE standard input window for the first calibration standard
appears.

Calibration
3 Carry out a two-point calibration according to the user guid-
ance (see page 104).
As soon as a stable value is achieved for the second standard
calibration solution, the slope (mV) and evaluation (Error, +++)
of the electrode after calibration is displayed.
The infotext, Accept calibration and terminate is displayed.
4 Using [OK], confirm the infotext Accept calibration and termi-
nate.
The calibration record is displayed and output to the interface.
The calibration procedure is finished.
5 Close the calibration record with [OK].
The ISE method: The Standard addition window pops up. The
Sample register is displayed.

Measuring

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6 In the Sample register, enter the values for the sample volume
(Sample volume) and the volume of the ISA/TISAB solution
(ISA/TISAB volume) as necessary.
7 Thoroughly rinse the electrode with deionized water.
8 Immerse the electrode in the sample.
9 Start the measurement with [Continue] .
The measured value is checked for stability (Stability control).
10 Wait for the measurement with stability control to end.
or
Take over the calibration value with [OK].
The Standard register with the calculated measured value
pops up.

11 In the Standard register, enter the values for Standard volume

and Standard concentration if necessary.
12 Add the standard solution to the sample.
13 Start the measurement with [Continue].
The measured value is checked for stability (Stability control).
14 Wait for the measurement with stability control to end.
or
Take over the calibration value with [OK].
The Calculation register with the calculated measured value
pops up.

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15 Change the displayed unit of the measured value with [Unit] if

necessary.
16 If required, display the record of the current measurement with
[Record].
17 Start measuring further samples with [Continue].
Repeat the steps 5 - 15 for all samples.
or
Terminate the measuring method with [Cancel] and confirm the
safety query with [OK].
Measurement with the selected method is completed.

9.6.3 Double standard addition

In the Double standard addition procedure, a known amount of stan-

dard solution is added to the sample in two steps.
First, one percent of the sample volume is added, then 2 percent of the
sample volume is added.
The ion concentration in the sample is calculated from the change in
potential between the first and second addition of standard solution.

Measuring
1 Check the currently displayed ion.
If the ion to be measured is displayed incorrectly:
Select the ion type in the menu, Measuring / ISE methods / Ion
type.
2 In the Measuring / ISE methods menu, select the method, Dou-
ble standard addition.
The ISE method: The Double standard addition window pops
up.
The Sample register is open.

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3 In the Sample register, enter the values for the sample volume
(Sample volume) and the volume of the ISA/TISAB solution
(ISA/TISAB volume) as necessary.
4 Thoroughly rinse the electrode with deionized water.
5 Immerse the electrode in the sample.
6 Start the measurement with [Continue] .
After end of the measurement, the Standard pops up.

7 In the Standard register, enter the value for Standard concen-

tration if necessary.
8 Add standard solution to the sample (1 percent by volume of
the sample solution).
9 Start the measurement with [Continue].
The measured value is checked for stability (Stability control).

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10 Wait for the measurement with stability control to end.

or
Take over the measured value with [OK].
The Standard register opens up.
11 Add standard solution to the sample (2 percent by volume of
the sample solution).
12 Start the measurement with [Continue].
The measured value is checked for stability (Stability control).
13 Wait for the measurement with stability control to end.
or
Take over the measured value with [OK].
The Calculation register with the calculated measured value
pops up.

14 Change the displayed unit of the measured value with [Unit] if

necessary.
15 If required, display the record of the current measurement with
[Record].
16 Start measuring further samples with [Continue].
Repeat the steps 2 - 14 for all samples.
or
Terminate the measuring method with [Cancel] and confirm the
safety query with [OK].
Measurement with the selected method is completed.

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9.6.4 Standard subtraction

In the "Standard Subtraction" procedure, a known amount of standard

solution is added to the sample (as complexing agent or precipitating
agent) and, thus, the ion concentration lowered.
The ion concentration in the sample is calculated from the change in
potential.

1 Check the currently displayed ion.

If the ion to be measured is displayed incorrectly:
Select the ion type in the menu, Measuring / ISE methods / Ion
type.
2 In the Measuring / ISE methods menu, select the method,
Standard subtraction.

Calibration
3 Carry out a two-point calibration according to the user guid-
ance (see page 104).
As soon as a stable value is achieved for the second standard
calibration solution, the slope (mV) and evaluation (Error, +++)
of the electrode after calibration is displayed.
The infotext, Accept calibration and terminate is displayed.
4 Using [OK], confirm the infotext Accept calibration and termi-
nate.
The calibration record is displayed and output to the interface.
The calibration procedure is finished.
5 Close the calibration record with [OK].
The ISE method: The Standard subtraction window pops up.
The Sample register is displayed.

Measuring

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6 In the Sample register, enter the values for the sample volume
(Sample volume) and the volume of the ISA/TISAB solution
(ISA/TISAB volume) as necessary.
7 Check the currently displayed ions.
If the ion of the standard (e.g. Cl (Sub) is displayed incorrectly:
Change the ion type of the standard solution [e.g. Cl (Sub)] in
the ION register.
8 Thoroughly rinse the electrode with deionized water.
9 Immerse the electrode in the sample.
10 Start the measurement with [Continue] .
The measured value is checked for stability (Stability control).
11 Wait for the measurement with stability control to end.
or
Take over the measured value with [OK].
The Standard register opens up.

12 In the Standard register, enter the values for Standard volume

and Standard concentration if necessary.
13 Add the standard solution to the sample.
14 Start the measurement with [Continue].
The measured value is checked for stability (Stability control).
15 Wait for the measurement with stability control to end.
or
Take over the measured value with [OK].
The Calculation register with the calculated measured value
pops up.

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16 Change the displayed unit of the measured value with [Unit] if

necessary.
17 If required, display the record of the current measurement with
[Record].
18 Start measuring further samples with [Continue].
Repeat the steps 5 - 16 for all samples.
or
Terminate the measuring method with [Cancel] and confirm the
safety query with [OK].
Measurement with the selected method is completed.

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9.6.5 Sample addition

In the Sample addition procedure, a known amount of test sample is

added to the standard solution.
The ion concentration in the sample is calculated from the change in
potential.

1 Check the currently displayed ion.

If the ion to be measured is displayed incorrectly:
Select the ion type in the menu, Measuring / ISE methods / Ion
type.
2 In the Measuring / ISE methods menu, select the method,
Sample addition.

Calibration
3 Carry out a two-point calibration according to the user guid-
ance (see page 104).
As soon as a stable value is achieved for the second standard
calibration solution, the slope (mV) and evaluation (Error, +++)
of the electrode after calibration is displayed.
The infotext, Accept calibration and terminate is displayed.
4 Using [OK], confirm the infotext Accept calibration and termi-
nate.
The calibration record is displayed and output to the interface.
The calibration procedure is finished.
5 Close the calibration record with [OK].
The ISE method: The Sample addition window pops up. The
Standard register is displayed.

Measuring

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6 In the Standard register, enter the values for Standard volume-

Standard concentration and ISA/TISAB volume if necessary.
7 Thoroughly rinse the electrode with deionized water.
8 Immerse the electrode in the standard.
9 Start the measurement with [Continue].
The measured value is checked for stability (Stability control).
10 Wait for the measurement with stability control to end.
or
Take over the measured value with [OK].
The Sample register opens up.

11 Add the sample to the standard solution.

12 In the Sample register, enter the values for the sample volume
(Sample volume) if necessary.
13 Start the measurement with [Continue].
The measured value is checked for stability (Stability control).
14 Wait for the measurement with stability control to end.
or
Take over the measured value with [OK].
The Calculation register with the calculated measured value
pops up.

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15 Change the displayed unit of the measured value with [Unit] if

necessary.
16 If required, display the record of the current measurement with
[Record].
17 Start measuring further samples with [Continue].
Repeat the steps 5 - 15 for all samples.
or
Terminate the measuring method with [Cancel] and confirm the
safety query with [OK].
Measurement with the selected method is completed.

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9.6.6 Sample subtraction

In the Sample subtraction procedure, a known amount of test sample

is added to the standard solution.
The ion concentration in the sample is calculated from the change in
potential.
The sample subtraction is one method for the indirect determination of
ions. It is used for the determination of ions for which no direct determi-
nation is possible.

1 Check the currently displayed ion.

If the ion to be measured is displayed incorrectly:
Select the ion type in the menu, Measuring / ISE methods / Ion
type.
2 In the Measuring / ISE methods menu, select the method,
Sample subtraction.

Calibration 3 Carry out a two-point calibration according to the user guid-

ance (see page 104).
As soon as a stable value is achieved for the second standard
calibration solution, the slope (mV) and evaluation (Error, +++)
of the electrode after calibration is displayed.
The infotext, Accept calibration and terminate is displayed.
4 Using [OK], confirm the infotext Accept calibration and termi-
nate.
The calibration record is displayed and output to the interface.
The calibration procedure is finished.
5 Close the calibration record with [OK].
The ISE method: The Sample subtraction window pops up.
The Standard register is displayed.

Measuring

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6 In the Standard register, enter the values for Standard volume-

Standard concentration and ISA/TISAB volume if necessary.
7 Check the currently displayed ions.
If the ion of the standard (e.g. Cl (Sub) is displayed incorrectly:
Change the ion type of the standard solution [e.g. Cl (Sub)] in
the ION register.
8 Thoroughly rinse the electrode with deionized water.
9 Immerse the electrode in the sample.
10 Start the measurement with [Continue] .
The measured value is checked for stability (Stability control).
11 Wait for the measurement with stability control to end.
or
Take over the measured value with [OK].
The Sample register opens up.

12 Add the sample to the standard solution.

13 In the Sample register, enter the value for the sample volume
(Sample volume) if necessary.
14 Start the measurement with [Continue].
The measured value is checked for stability (Stability control).
15 Wait for the measurement with stability control to end.
or
Take over the measured value with [OK].
The Calculation register with the calculated measured value
pops up.

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16 Change the displayed unit of the measured value with [Unit] if

necessary.
17 If required, display the record of the current measurement with
[Record].
18 Start measuring further samples with [Continue].
Repeat the steps 5 - 16 for all samples.
or
Terminate the measuring method with [Cancel] and confirm the
safety query with [OK].
Measurement with the selected method is completed.

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9.6.7 Blank value addition (Standard addition with

blank value correction)

In the "Standard addition with blank value correction" procedure, a

known amount of standard solution is added to the sample in two steps.
With the first addition, the ion concentration is raised to the linear range
of the electrode characteristic curve.
The second addition is equivalent to the standard addition.
The ion concentration in the sample is calculated from the change in
potential.

1 Check the currently displayed ion.

If the ion to be measured is displayed incorrectly:
Select the ion type in the menu, Measuring / ISE methods / Ion
type.
2 In the Measuring / ISE methods menu, select the method,
Blank value addition.

Calibration
3 Carry out a two-point calibration according to the user guid-
ance (see page 104).
As soon as a stable value is achieved for the second standard
calibration solution, the slope (mV) and evaluation (Error, +++)
of the electrode after calibration is displayed.
The infotext, Accept calibration and terminate is displayed.
4 Using [OK], confirm the infotext Accept calibration and termi-
nate.
The calibration record is displayed and output to the interface.
The calibration procedure is finished.
5 Close the calibration record with [OK].
The ISE method: The Blank value addition window pops up.
The Sample register is displayed.

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Measuring

6 In the Sample register, enter the following values:

z the volume of the sample (Sample volume)
z the volume of the ISA/TISAB solution (ISA/TISAB volume)
z the volume of the blank value solution (Blank value volume)
z the concentration of the blank value solution (Blank value
conc.).
7 Thoroughly rinse the electrode with deionized water.
8 Immerse the electrode in the sample that was supplemented
with blank value solution.
9 Start the measurement with [Continue] .
The measured value is checked for stability (Stability control).
10 Wait for the measurement with stability control to end.
or
Take over the measured value with [OK].
The Standard register opens up.

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11 In the Standard register, enter the values for Standard volume

and Standard concentration if necessary.
12 Add the standard solution to the sample.
13 Start the measurement with [Continue].
The measured value is checked for stability (Stability control).
14 Wait for the measurement with stability control to end.
or
Take over the measured value with [OK].
The Calculation register with the calculated measured value
pops up.

15 Change the displayed unit of the measured value with [Unit] if

necessary.
16 If required, display the record of the current measurement with
[Record].
17 Start measuring further samples with [Continue].
Repeat the steps 5 - 15 for all samples.
or
Terminate the measuring method with [Cancel] and confirm the
safety query with [OK].
Measurement with the selected method is completed.

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10 Memory
With the ProLab 3000 software you can store and recall all important
measurement and configuration data.

Each data type has an individual storage format.

z Measurement datasets of the manual memory "*.mst" (see
page 130)
z Measurement datasets of the automatic memory "*.ast" (see
page 132)
z Recorder "*.rdt" (see page 139)
z Configurations "*.ste" (see page 158)
z Calibration histories "*.cal" (see page 136)

10.1 Measurement data

You can save measurement data in the following ways:

z Manual
– with <STO>:
stores all current measured values
z Automatically
– after each measurement with manual stability control.
– within the specified interval of the automatic memory (see
page 132)

When creating an automatic memory, the system each time prompts

you to create a file. The file is stored in the directory of the user. An
external USB medium appears in the directory of the user and can be
selected as the location for the automatic memory.

For measurement data to stored manually, you can create or select a

file prior to storing.
If no file is selected prior to storing, the ProLab 3000 automatically
stores the measurement data in the files last selected by the current
user. If this user has never selected a file, one is created automatically.

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10.1.1 Creating and selecting a file for manually

stored measurement datasets

Creating a file for You can create the file in which to store your manually stored measure-
manually stored ment datasets.
measurement datasets Files for manually stored measurement datasets have the file exten-
sion, "*.mst".

1 Open the menu, Memory / Manual memory / New....

The Open file dialog box pops up.
2 Enter a new file name and confirm with [OK].
The file is created. Data from manual storing will be stored in it.

Selecting the file for You can open an existing file for stored measurement datasets in order
measurement dataset to to append new measurement datasets.
be stored manually Files for manually stored measurement datasets have the file exten-
sion, "*.mst".

Note
If a user has not yet created a file, the data is stored in the file, "Man-
Sto.mst".

1 Open the menu, Memory / Manual memory / Select....

The Open file dialog box pops up.
2 Select a file and confirm with [OK].
Manually stored data is written in the file.
New datasets are appended to the existing dataset.

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10.1.2 Storing measurement datasets manually

Measurement datasets are stored in a file selected before (see

page 130). Allocating an Identification (ID) to the data record makes it
easier to find it again.

1 Using <STO>, display the current measurement dataset.

2 If necessary, enter an ID (ID) for the measurement.

3 If necessary, enter additional text in the comment field (V).

Note
The entry in the ID field automatically appears for the next storing pro-
cedure.

4 Use [OK] to save the selected data.

The window is closed.

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10.1.3 Automatically storing measurement datasets

The file in which measurement datasets should be automatically stored

is selected by yourself. You can create a new file o4r select an existing
file to be overwritten.
Files for automatically stored measurement datasets have the file
extension, "*.ast".

1 Open the menu, Memory / Automatic memory / New....

The New file dialog is open.
2 Enter a new file name and confirm with [OK].
The Automatic memory window opens.
3 If necessary, select a different interval in the Save interval list.

Note
The selection in the Save interval field automatically appears for the
next storing.
One hour is preset as the storing duration.

4 Enter the final date for automatic storing (date and time) and
confirm with [OK].
The window for the input of the ID (ID) and comment (V) pops
up.
5 If necessary, enter an ID (ID) for the measurement and a text
in the comment field (V).

Note
The entry in the ID field automatically appears for the next storing pro-
cedure.

6 Start the automatic storing with [OK].

The Automatic memory window pops up.
Other functions cannot be executed while the automatic storing
is active.

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Note
The progress of the automatic memory is indicated by a progress bar.
The Automatic memory window can be minimized with the mouse in
order to see the measured value display.

7 Wait for the automatic storing to be finished.

or
Finish the automatic storing manually with [Terminate].

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10.1.4 Displaying and filtering stored measurement datasets

1 Open the currently selected manual memory with <RCL>.

or
Open the menu, Memory / Manual memory or Automatic mem-
ory / Display....
The Open file dialog box pops up.
2 Select a file and confirm with [OK].
A data record of the selected file is displayed.

3 Using [Filter], open the Data filter window for the setting of filter
criteria if necessary.
A combination of the following criteria is possible as the filter:
– Memory no.
– Identification
– Date of storing

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4 Activate the selected filter criteria with [OK].

The [Filter] option field is marked.
The filter criteria are active.
5 Enter or select a number in the selection field for the dataset
number.
The selected dataset within the list of (filtered) measurement
datasets is displayed.
6 If necessary, download the list of the (filtered) measurement
dataset to the interface with [Print].
7 Terminate the display of stored measurement datasets with
Terminate.

10.1.5 Erasing measurement datasets

1 Open the menu, Memory / Manual memory or Automatic mem-

ory / Delete....
The Delete file dialog box pops up.
2 Select the file to be deleted and confirm with [OK].
3 Confirm the safety query with [OK].
The file is deleted.

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10.2 Calibration data

The current calibration record is automatically stored after each valid
calibration procedure.

10.2.1 Displaying stored calibration data

The current calibration record for a meter and a sensor can be found in
Displaying the current the menu, Calibration / Calibration record....
calibration record
1 Highlight a channel in the measured value display.
2 Select the measured parameter with <MODE>.
3 Open the Calibration menu with Calibration record....
The current calibration record is displayed.
4 If required, output the calibration data to the interface with
[Print].

Note
The current calibration record can be quickly displayed by clicking on
the CalClock with the right mouse button.

Displaying the
1 Open the Calibration / Calibration history... menu.
calibration history
The Calibration memory window with the calibration data for
the selected sensor pops up.
2 If required, output the calibration data to the interface with
[Print].

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10.2.2 Managing the calibration data

The calibration data is stored in the current calibration record (menu,

Calibration / Calibration record...) and in the calibration history (menu,
Calibration / Calibration history... and Memory / Calibration memory).

The current calibration record of ID sensors is stored in the sensor, of

non ID sensors it is stored in the ProLab 3000.

The meter creates a separate calibration history for each ID sensor cal-
ibrated with the ProLab 3000.
For non ID sensors calibrated with the meter, a calibration history is
created for each measured parameter (pH, ISE) where the current cal-
ibration data is appended.

Managing functions for all calibration data are available to the adminis-
trator only.

Viewing the calibration The administrator can view the calibration histories of all sensors and
history users.

1 Open the menu, Memory / Calibration memory / Display....

The Open file dialog box pops up.
2 If necessary, select a different folder.
3 Select a file ("*.cal") and confirm with [OK].
The Calibration memory window with the calibration data pops
up.
4 If necessary, output the calibration data to the interface with
[Print].

Erasing a calibration The administrator can delete the calibration histories of all sensors and
history users.
The current calibration data of each sensor is stored separately and is
retained even if the calibration history is deleted.

1 Open the menu, Memory / Calibration memory / Delete....

The Delete file dialog box pops up.
2 If necessary, select a different folder.
3 Select a file ("*.cal") and confirm with [OK].
A safely query pops up.
4 Confirm the deletion of the file with [OK].
The file is deleted.

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10.3 Recorder data

Information on the recording, storing, displaying and deleting of mea-
sured value curves is given on page 139 ff.
Files containing recorder data have the file extension "*.rdt".

10.4 Configuration data

Information on the recording, storing, displaying and deleting of config-
uration files is given on page 158 ff.
Files containing configuration data have the file extension "*.ste".

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11 Recorder
The recorder represents measured values graphically in a system of
coordinates. The recorder can record a running measurement
(online recorder) or the developing of a stored measurement
(offline recorder).

The following editing functions are available for the recorder:

z Displaying or hiding the measured temperature (see page 141)
z Adjusting the colors and axis intercepts of the recorder (see
page 144)
z Setting the limit values (see page 147)
z Zooming the displayed section (see page 149)
z Displaying a precise measured value at a specific location (see
page 151)

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11.1 Recording the measured value curve

The recorder records the course of the measured values curve and the
course of the temperature curve.

Starting the recorder

1 Highlight a channel in the measured value display,e.g. pH.
2 Open the Measuring / Recorder... menu.
The Online recorder window pops up.
The current measurement data of the selected channel is
recorded.

Note
While the recorder is recording measurement data of a channel, the
functions for this channel that could interfere with data integrity are
blocked.

If the recorders for two similar electrodes are activated, both mea-
sured value curves and both temperature value curves are displayed
in one window.

During the recording, the measured value graphic is stored in the

working memory only. When the recorder is terminated, a query
appears for the storing of the measured value graphic.

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11.2 Recording the curve of the measured

temperature value
If the recorder was started without recording the temperature value,
you can switch on the recording of the temperature value.
The current setting for the recording of the temperature value is
retained until the next start of the recorder.

1 Open the recorder menu, Settings....

The recording of the temperature value is switched off or on
( ).
2 Select the menu item, Temperature recording.
To change the setting for the temperature recording, it is
required to restart the online recorder.
A dialog box pops up prior to restarting where you can store the
previous recorder data.
3 Discard the previous recorder data with [Cancel].
The recording is restarted with the current setting for the
temperature value recording.
or
– Confirm the storing of the previous recorder data with [OK].
The Save file window opens.
– Enter a new file name and confirm with [OK].
The previous recorder data is stored.
The recording is restarted with the current setting for the
temperature value recording.

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11.3 Storing recorder data

The recorder records the course of the measured values curve and the
course of the temperature curve. You can store the previous course of
the measured value recording in a file at any time.

1 Open the File / Save as menu.

The Save file window pops up.
2 Enter a new file name and confirm with [OK].
The previous progression of the measured value graphic is
stored in the file.
With the online recorder, the progression of the measured
value is stored in the working memory.
3 Further storing processes:
Open the File / Save menu.
The recorder data is stored in the file that was selected
previously without any query.

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11.4 Displaying stored recorder data

Opening the offline

1 Open the menu, Memory / Recorder / Display....
recorder
The Open file dialog box pops up.
2 Select a file with recorder data and confirm with [OK].
The Offline recorder window pops up and displays the selected
recorder data.

Displaying a different
3 Open the File / Open menu.
recorder file
The Open file dialog box pops up.
4 Select a file and confirm with [OK].
The selected recorder data is displayed.

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11.5 Settings for the recorder

In the Recorder settings dialog box, the appearance of your recorder is
defined. The following settings can be done while the recording is run-
ning or after opening a completed recording.

z Colors for the recorder window (register, General)

– Background color of the system of coordinates
– Color of the legend of the system of coordinates
z Colors of the curves (register, Channels)
– Color of measured value curve (Measured value)
– Color of temperature value curve (Temp.)
z Scaling of the axis for the measured value (register, Measured
value)
– Axis type (linear / logarithmic)
– Minimum and maximum value for the recorder
– Activating the limit value function
– Minimum and maximum limit value for the measured value
z Scaling of the axis for the temperature value (register, Temperature)
– Minimum and maximum value for the recorder
– Activating the limit value function
– Minimum and maximum limit value for the temperature value
z Displayed intercept of the time axis (register, Time)
– Unit of the x axis (Displayed time period)
z Recording interval (register, Time)

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11.5.1 Defining the recording interval

The Recording interval defines at which intervals the measured values

are recorded.

1 Open the recorder menu, Settings...Settings....

The Recorder settings window pops up.
2 Select the Time register for the entry of the recording interval.
3 Select the interval.
4 Use [OK] to accept the new settings and close the dialog box,
Recorder settings.
The new settings are active.
or
Select another register to make further settings.

11.5.2 Specifying the axes of the system of coordinates

The appearance of the recorder is specified by specifying the axes of

the system of coordinates:
z Time axis of the measured value recording:
The time period entered in the field, Displayed time period is dis-
played including the last recorded measured value.
z Measured value and temperature value axis
– Axis type (linear or logarithmic)
– Resolution of the axis for measured values
The number in the Decimal places field specifies with how many
decimal places the measured value scale should be labeled.
– Minimum and maximum measured value
Here you define the part of the measured value and temperature
you want to keep in view such as pH 0 to pH 14.
– Scaling of the axes (Axis scale, rough and fine)
In the Axis scale, rough field you specify how often the measured
value scale should be divided between the minimum and maxi-
mum value.
In the fine field you specify how often an axis intercept of the
rough scaling should be divided.
You can view the entire progress of the recording regardless of the set-
tings at any time (see page 149).

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1 Open the recorder menu, Settings...Settings....

The Recorder settings window pops up.
2 Select the Measured value register for the scaling of the
measured value axis.
3 Make the settings for the axes.
If necessary, define limit values (see page 147).
4 Select the Temperature register for the scaling of the
temperature value axis.
5 Make the settings for the axes.
If necessary, define limit values (see page 147).
6 Use [OK] to accept the new settings and close the dialog box,
Recorder settings.
The new settings are active.
or
Select another register to make further settings.

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11.5.3 Defining and activating limit values

With the limit values you define the measured values at which the curve
of the measurement developing is displayed gray if the measured are
below or above the limits.
Thus measured values below or above the limits can easily be recog-
nized.

1 Open the recorder menu, Settings...Settings....

The Recorder settings window pops up.
2 Select the Measured value register to define the limit values for
the measured value.
3 Mark the selection field, Switch on limit value monitoring.
4 Enter the upper and lower limit value.
5 Select the Temperature register to define the limit values for
the measured value.
6 Mark the selection field, Switch on limit value monitoring.
7 Enter the upper and lower limit value.
8 Use [OK] to accept the new settings and close the dialog box,
Recorder settings.
The new settings are active.
or
Select another register to make further settings.

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11.5.4 Defining the colors for the recorder representation

The appearance of the recorder is determined with the following colors:

z Background color
z Text color for axes legend
z Color of the measured value curve
z Color of temperature value curve

1 Open the recorder menu, Settings...Settings....

The Recorder settings window pops up.
2 Select the General register card for the setting of background
color and text color.
3 Open the color palette for background color with [...].
4 Select a color and confirm with [OK].
5 Open the color palette for the text color with [...].
6 Select a color and confirm with [OK].

7 Select the Channels register for the setting of the curve colors.
8 If necessary, select a channel.
9 Open the color palette for the measured value (Measured
value) with [...].
10 Select a color and confirm with [OK].
11 Open the color palette for the temperature value (Temp.) with
[...].
12 Select a color and confirm with [OK].

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13 Use [OK] to accept the new settings and close the dialog box,
Recorder settings.
The new settings are active.
or
Select another register to make further settings.

11.6 Changing the displayed axis intercept

The standard settings for the displayed axis intercepts are defined in
the Recorder settings window for the time axis (register, Time), the
measured value axis (register, Measured value) and the temperature
axis (register, Temperature) (see page 145).

In the recorder window, you can change the displayed axis intercept
and move back to the standard display at any time.

Displaying the entire The Total time axis function provides an overview of the entire process
time axis since the recording of the measured value curve was started.

1 Open the Display / Total time axis menu.

The time axis from the start of the recording is displayed.

Displaying the time axis The Reset zoom function is used to reset the representation of the
with specified time recorder to the standard settings specified in the Recorder settings win-
period dow.
The recorder then shows the specified minimum and maximum of the
measured value and temperature axis and the specified time period
(see page 145).

1 Open the Display / Reset zoom menu.

The representation of the recorder is reset to the standard set-
tings specified in the window, Recorder settings.

Displacing the axis As described below, the axis intercepts of the recorder can be dis-
intercept placed either in the direction of the time axis or in the direction of the
measured value/temperature axis. By his the displayed axis intercept is
displaced to the left/right or up/down but not scaled up.
This function is only available if the zoom function is not active.

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1 Click on an arrow at the beginning or end of an axis with the

mouse (time axis or measured value/temperature axis).
The displayed axis intercept is displaced in the direction of the
arrow.

Zooming an axis The axis intercepts of the recorder can be zoomed as follows (time axis
intercept and measured value/temperature axis).

1 Draw a rectangle within the recorder with the mouse.

The marked section is displayed bigger.

Undoing the zooming You can undo the last zoom step as follows.

1 Click on an axis with the mouse.

The context menu pops up.
2 Open the menu item, Undo zoom.
The last zoom step is undone.

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11.7 Displaying exact measurement data at a point (cursor)

In the recorder window, you can display the exact measurement data
numerically at a point of the measuring curve.

1 Open the menu item, DisplayCursor.

A cursor is displayed.
2 Move the cursor to a position with the mouse.
The respective numeric measurement data is displayed in a
box.

11.8 Deleting stored recorder data

1 Open the menu, Memory / Recorder / Delete.... The Delete file

dialog box pops up.
2 Select a file ("*.rdt") and confirm with [OK].
A safely query pops up.
3 Confirm the safety query with [OK].
The file is deleted.

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12 Transmitting data
12.1 Printing current measurement data
To which interface the measurement data should be output with
<PRINT> is specified in the menu, System / Data output... (see
page 60).

1 Output the current measurement data to the interface with

<PRINT>.

12.2 Transmitting data (to a PC or printer)

The meter has the following interfaces for PC/printer:
z RS232 interface (serial interface)
e.g. to connect a PC/printer with the serial interface
z USB-B interfaceUSB Device
e.g. to connect a PC
z USB-A interface, USB Host
e.g. to connect devices with USB connection (printer, keyboard,
mouse, storage, card reader, barcode scanner, hub etc.)

The interfaces, RS232 (serial port) and USB-B (USB Device) are used
to transmit data to a PC and update the meter software.

The USB-A (USB Host) also enables the transmission of data to an

external USB printer.

12.2.1 RS232 interface

1 Connect the interface to the PC with the Z390 (PC) cable or to

the printer with the Z393 (ext. serial printer) cable.
2 Set up similar transmission data on the PC/printer:
z Baud rate: selectable in the range 1200 ... 115200
z Handshake: RTS/CTS + Xon/Xoff
z Set on the PC only:
– Parity: none
– Data bits: 8
– Stop bits: 1

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Note
Pin assignment of the socket: See page 169.

12.2.2 USB-B interface (USB Device)

Connect the interface to the PC via the supplied Z875 USB cable.

Installation of the USB System requirements of the PC for installation of the USB driver:
driver on the PC
z PC with Pentium processor or higher with at least one free USB con-
nection and CD-ROM drive
z Windows 2000, XP.

1 Insert the supplied installation CD in the CD drive of your PC.

2 Connect the meter to the USB-B input (USB Device) of the PC
via the USB cable.
Windows automatically installs the driver for the meter. Follow
the Windows installation instructions as necessary.
The meter is listed as a virtual COM interface among the con-
nections in the Windows instrument manager.
3 Set up similar transmission data on the PC/printer:
z Baud rate: selectable in the range 1200 ... 115200
z Handshake: RTS/CTS + Xon/Xoff
z Set on the PC only:
– Parity: none
– Data bits: 8
– Stop bits: 1

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12.2.3 USB-A interface (USB Host)

Connect the USB-A interface (USB Host) of the ProLab 3000 to the
printer.

1 Connect the USB printer to the USB Host interface.

12.2.4 Options for data transmission

Data can be transmitted to a PC or external printer via the interfaces,

RS232, USB-B (USB Device) and USB-A (USB Host).
The following table shows which data is transmitted to the interface in
which way:

Data Control Operation / description

Current measured Manual z With <PRINT>.
values of the con- z Simultaneously with every
nected sensor manual storage process with
<STO> (see page 129).
automatic, at z Simultaneously with every
intervals automatic storage process
(see page 129).
Stored measured Manual z Displayed dataset:
values with <PRINT> after recall
from data storage.
z All datasets correspond to the
filter criteria with the [Print]
function in the memory dis-
play
Calibration Manual z Displayed calibration record:
records with <PRINT> after recall
from data memory.
Automatically z For the respective sensor at
the end of a calibration proce-
dure.

Note
The following rule applies: Except for the menus, the display content
is generally downloaded to the interface with <PRINT> (displayed
measured values, measurement datasets, calibration records).

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12.3 Data backup

12.3.1 Data backup by the administrator

To back up important data against being lost or to relocate big files from
the instrument, the administrator can plug an external USB medium to
the ProLab 3000 in order to save data on it.

1 Connect a USB memory device to the USB-A USB Host inter-

face.
2 Open the File / Data saving... menu.
A double dialog box opens.

3 Select a file extension in the list of file types, e.g. *.sto.

All files in this directory that have the selected extension are
displayed.
4 In the source directory, select the source to be copied, a direc-
tory (CalHistory, or a user directory) or a file.
5 In the target directory, select the target, such as a directory in
an external storage medium (hard disk).
6 If necessary, create and select a new folder in the target direc-
tory.
7 With [>>>] start the copy procedure.
8 If necessary, back up further files or directories.
9 Terminate the data backup with <ESC>.

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12.3.2 Viewing backed up data

The external USB medium is displayed within the directory of each

user. Thus the data can be read at any time after connecting the USB
medium.

Example: Viewing backed up measurement data

1 Connect a USB memory device to the USB-A USB Host inter-

face.
2 Open the menu, Memory / Manual memory or Automatic mem-
ory / Display....
The Open file dialog box pops up.
3 Select a file from the external medium and confirm with [OK].
A data record of the selected file is displayed.

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13 Configurations
As the administrator, you can create and assign a configuration to each
user (see page 52). The settings for each measured parameter are
defined in a configuration file. If a configuration file is assigned to a
user, the measurement setting for the user are fixed and the user rights
limited.

All users whose user rights were not limited by the user can create their
own configuration files for different measurement tasks. This can be
useful if measurement tasks require different basic settings.

After a user login without any stored configuration, the meter always
starts with the settings last used (not with the configuration last called
up).

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13.1 Viewing a configuration

A configuration viewer is available so the current configuration settings
can be viewed. It gives an overview of the current measurement set-
tings.

1 Open the menu, File / View configuration....

The Configuration window appears.
The current configuration is displayed.

Note
A user whose user rights were restricted by the administrator cannot
change these settings.

2 If required, output the settings to the interface with [Print].

3 Close the Configuration window with [Terminate].

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13.2 Creating a new configuration

When creating a configuration, the current settings of the meter for
each measured parameter are taken over in the Configuration window
first. Then the settings can be adjusted and stored as an extra configu-
ration file.

1 Open the menu, File / New configuration....

The Configuration window appears.

2 Define the options for allowed measurements for all measured

parameters.
3 Using [Save], open the Save file dialog.
4 Enter a new file name and confirm with [OK].
The configuration is stored.
5 If required, output the settings to the interface with [Print].
6 If necessary, store further configurations.
7 Close the Configuration window with [Terminate].

Note
To activate the settings of a configuration, the configuration has to be
loaded (see page 161).

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13.3 Loading a configuration

All users who can create a configuration can also load it.
When loading a configuration, all settings stored in the configuration file
can be loaded.

1 Open the File menu with Select configuration....

The Open file dialog box pops up.
2 Select a configuration file ("*.ste") and confirm with [OK].
The Configuration window pops up.

3 The configuration for all measured parameters can be viewed

but not changed.
4 If required, output the settings to the interface with [Print].
5 Load the configuration with [OK].
The settings stored in the configuration are active.
6 Close the Configuration window with [Terminate].

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13.4 Deleting a configuration

All users who can create a configuration can also delete it in their direc-
tory.

1 Open the File menu with Delete configuration....

The Delete file dialog box pops up.
2 Select the file to be deleted and confirm with [OK].
3 Confirm the safety query with [OK].
The file is deleted.

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14 Maintenance, cleaning, disposal

14.1 Maintenance
The only maintenance activity required is replacing the batteries.

Note
For maintenance of the electrodes refer to the relevant operating man-
uals.

Replacing the batteries If the meter stays connected to the power supply while the batteries are
being replaced, or if they are replaced within approx. one minute, the
date and time will be retained in the meter.

1 Using a screw driver, unscrew the screws (2) of the lid of the
battery compartment.

2 Open the battery compartment (1) on the underside of the

meter.
3 Remove the four batteries from the battery compartment.
4 Place four new batteries (type Micro AAA) in the battery com-
partment.

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5 Close the battery compartment (1).

6 Using a screw driver, tighten the screws (2) of the lid of the bat-
tery compartment.
7 Switch the meter on with <On/Off>.

Note
If the date and time are lost, the setting of the date and time is dis-
played automatically after switching on.
Set the date and time (see page 59)

CAUTION
Make sure that the poles of the batteries are the right way round.
The ± signs on the batteries must correspond to the ± signs in
the battery compartment.
Only use leakproof alkaline manganese batteries.

14.2 Cleaning
Occasionally wipe the outside of the meter with a damp, lint-free cloth.
Disinfect the housing with isopropanol as required.

CAUTION
The housing components are made out of synthetic materials
(polyurethane, ABS, PMMA), metal and glass. Thus, avoid con-
tact of the synthetic material with acetone and similar detergents
that contain solvents. Remove any splashes immediately.

14.3 Disposal

Note
This instrument contains batteries. Batteries that have been removed
must only be disposed of at the recycling facility set up for this purpose
or via the retail outlet.
It is illegal to dispose of the instrument in household refuse.

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15 What to do if...
15.1 pH and ORP measurement

Error message Err1 Cause Remedy

pH electrode:
– Measured value outside the – Use a suitable electrode
measuring range
– Air bubble in front of the dia- – Remove air bubble
phragm
– Air in the diaphragm – Extract air or moisten junc-
tion
– Cable broken – Replace the electrode
– Gel electrolyte dried out – Replace the electrode

Error message Err2 Cause Remedy

– No electrode connected – Connect the electrode
– Setting time during calibration – Adjust temperature if neces-
too long sary
– Recalibrate

Error message Err4 Cause Remedy

– Temperature not stable during – Adjust temperature if neces-
calibration. sary
– Recalibrate

Error message, Error Cause Remedy

pH electrode:
– The values determined for zero – Recalibrate
point and slope of the electrode
are outside the allowed limits.
– Diaphragm contaminated – Clean diaphragm

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Cause Remedy
– Electrode broken – Replace the electrode
Buffer solutions:
– Incorrect buffer solutions – Select the suitable buffer set
for the buffers used
or
– Replace the buffer solutions
– Buffer solutions too old – Use only once.
Note the shelf life
– Buffer solutions depleted – Replace the buffer solutions

No stable measured Cause Remedy

value
pH electrode:
– Diaphragm contaminated – Clean diaphragm
– Membrane contaminated – Clean membrane
Test sample:
– pH value not stable – Measure with air excluded if
necessary
– Temperature not stable – Adjust temperature if neces-
sary
Electrode + test sample:
– Conductivity too low – Use a suitable electrode
– Temperature too high – Use a suitable electrode
– Organic liquids – Use a suitable electrode

Obviously incorrect Cause Remedy

measured values
pH electrode:
– Not connected – Connect the electrode
– Cable broken – Replace the electrode
– pH electrode unsuitable – Use a suitable electrode
– Temperature difference – Adjust temperature of buffer
between buffer and test sam- or sample solutions
ple too high

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Cause Remedy
– Measurement procedure not – Follow special procedure
suitable

CalClock on red Cause Remedy

background
– Calibration interval expired – Recalibrate the measuring
system

15.2 Ion selective measurement

Error message Err1 Cause Remedy

– Measuring range exceeded – Dilute test sample

Obviously incorrect Cause Remedy

measured values
– Electrode not connected – Connect the electrode
– Cable broken – Replace the electrode

Error message, Error Cause Remedy

(invalid calibration)
ISE electrode:
– Moisture in the plug – Dry plug
– Electrode obsolete – Replace the electrode
– Electrode unsuitable for the – Use a suitable electrode
range to be measured
– Socket damp – Dry socket
Calibration procedure:
– Wrong sequence of standards – Select correct sequence
for three point calibration
– Calibration standards do not – Adjust the temperature of
have the correct temperature the calibration standards
(max. ± 2 °C temperature differ-
ence)

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Warning [TempErr] Cause Remedy

– Temperature difference – Adjust the temperature of
between measurement and cali- the test sample
bration greater than 2 K.

Warning [ISEErr] Cause Remedy

– Electrode voltage outside cali- – Recalibrate
brated range

15.3 General errors

Meter does not react to Cause Remedy

keystroke
– Operating condition undefined – Processor reset:
or EMC load unallowed Press the <On/Off> and
<SC> key simultaneously.

Time is lost Cause Remedy

– Buffer batteries empty – Exchange buffer batteries
(see page 163)

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16 Technical Data
16.1 General data

Dimensions approx. 240 x 280 x 70 mm

Weight approx. 2.5 kg (without power pack)
Mechanical structure Type of protection IP 54
Electrical safety Protective class III
Test certificates cETLus, CE

Ambient Storage - 25 °C ... + 65 °C

conditions
Operation + 5 °C ... + 45 °C
Climatic class 2

Power Power pack FRIWO FW7555M/09, 15.1432.500-00

supply Friwo Part. No. 1883259
Input: 100 ... 240 V ~ / 50 ... 60 Hz / 400 mA
Output: 9 V = / 1.5 A
Connection max. overvoltage category II
Primary plugs contained in the scope of
delivery: Euro, US, UK and Australian.
Batteries 4 x 1.5 V alkali-manganese batteries, Type
(to buffer the system AAA
clock in the case of a
power failure)

Sensor input pH/U/ISE probe 1 + 2

Input resistance > 5 E12 Ohm
Input current < 1 E-12 A

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RS232 serial interface Connection of the PC cable (Z390) or printer cable (Z393)
Baud rate adjustable:
1200, 2400, 4800, 9600, 19200 Baud
Type RS232, data download
Data bits 8
Stop bits 2
Parity None
Handshake RTS/CTS
Cable length Max. 15m
Socket assignment 1 RTS
3
2
2 RxD
4
6 3 TxD
5 1 4-
5 SG
RS 232 6 CTS

USB-B interface (USB Automatic switch-over when a USB cable is connected

Device)
Type RS232 - USB interface converter
Cable length max. 3 m

USB-A interface (USB

Host) Type USB 1.1
Cable length max. 3 m

Guidelines EMC EC guideline 2004/108/EC

and norms used
Interference emission:
EN 61326-1
EN 61000-3-2
EN 61000-3-3
FCC Class A
Interference immunity:
EN 61326-1
Performance feature C for ESD according to
IEC61000-4-2

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Instrument safety EC guideline 2006/95/EC

EN 61010-1 class A
ANSI/UL 61010-1
CAN/CSA-C22.2 No. 61010-1
Radio data transmis- EC guideline 1999/5/EC
sion EN 300 330-2
EN 50364
EN 60950-1
Climatic class VDI/VDE 3540
IP protection class EN 60529

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16.2 Measuring ranges, resolution, accuracy

16.2.1 Temperature

Measuring ranges, Variable Measuring range Resolution

resolution
T [°C] - 35.0 ... + 150.0 0.1

Accuracy (± 1 digit) Variable Accuracy Temperature of the

test sample
T [°C] ± 0.1 - 35.0 ... + 150.0

Manual Variable Range Increment

temperature input
Tmanual [°C] -35 ... + 150 1

16.2.2 pH/ORP

Measuring ranges, Variable Measuring range Resolution

resolution
pH - 2.000 ... + 20.000 0.001
- 2.00 ... + 20.00 0.01
- 2.0 ... + 20.0 0.1
U [mV] - 2200.0 ... + 2200.0 0.1
- 2200 ... + 2200 1

Accuracy (± 1 digit) Variable Accuracy Temperature of the test

sample
pH ± 0.002 + 15 °C ... + 35 °C
± 0.01
± 0.1

U [mV] / range
- 2200.0 ... + 2200.0 ± 0.1 + 15 °C ... + 35 °C
- 2200 ... + 2200 ±1 + 15 °C ... + 35 °C

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16.2.3 ISE

Measuring ranges, Variable Measuring range Resolution

resolution
ISE 1.0 E-40 ... 9.9 E-4 1 E-39 ... 1 E-5
[mg/l, %, ppm, 0.001 ... 9.999 0.001
mg/kg, µmol/l] 10.00 ... 99.99 0.01
100.0 ... 999.9 0.1
1000 ... 999999 1
1.0 E6 ... 9.9 E39 1 E1 ... 1 E38

Manual Variable Range Increment

temperature input
Tmanual [°C] - 35 ... + 150 1

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17 Lists
This chapter provides additional information and orientation aids.

Abbreviations The list of abbreviations explains the indicators and abbreviations that
appear on the display and in the manual.

Status display indica-

tors

Specialist terms The glossary briefly explains the meaning of the specialist terms. How-
ever, terms that should already be familiar to the target group are not
described here.

Trademarks used The list comprises the trademarks used in the present document and
their owners.

Index The index will help you to find the topics that you are looking for.

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Abbreviations

°C Temperature unit, degrees Celsius

°F Temperature unit, degrees Fahrenheit
LoBat Batteries almost empty (Low battery)
mV Voltage unit
mV/pH Unit of the electrode slope (internat. mV)
pH pH value
S Slope (internat. k)
SELV Safety Extra Low Voltage
U Voltage

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Status display indicators

[SC] Measured value (Hold) or

Measurement with stability control
[TP] Temperature sensor is being used
[ISEErr] For calibration with ion sensitive electrodes only:
Electrode voltage outside the calibrated range (see
page 167)

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Glossary

Adjusting To manipulate a measuring system so that the relevant value (e. g.

the displayed value) differs as little as possible from the correct value
or a value that is regarded as correct, or that the difference remains
within the tolerance.
Calibration Comparing the value from a measuring system (e. g. the displayed
value) to the correct value or a value that is regarded as correct. Of-
ten, this expression is also used when the measuring system is ad-
justed at the same time (see adjusting).
Channel A channel is an indication on the display that corresponds to a phys-
ical connection at the meter.
The connections for similar sensor types are numbered on the meter
(pH/U/ISE Probe 1, pH/U/ISE Probe 2). To secure the assignment of
connection and channel on the display, the channel is numbered on
the display(e.g. pH1 and U2).
Electrode zero point The zero point of a pH electrode is the pH value at which the electro-
motive force of the pH electrode at a specified temperature is zero.
Normally, this is at 25 °C.
Electromotive force of an The electromotive force (voltage) U of the electrode is the measur-
electrode able electromotive force of an electrode in a solution. It equals the
sum of all the galvanic voltages of the electrode. Its dependency on
the pH results in the electrode function, which is characterized by the
parameters, slope and zero point.
Junction The junction is a porous body in the housing wall of reference elec-
trodes or electrolyte bridges. It forms the electrical contact between
two solutions and makes electrolyte exchange more difficult. The ex-
pression, junction, is also used for ground or junction-less transi-
tions.
Measured parameter The measured parameter is the physical dimension determined by
measuring, e. g. pH.
Measured value The measured value is the special value of a measured parameter
to be determined. It is given as a combination of the numerical value
and unit (e. g. 3 m; 0.5 s; 5.2 A; 373.15 K).
Molality Molality is the quantity (in Mol) of a dissolved substance in 1000 g
solvent.
Offset potential The measurable potential of a symmetrical electrode, the membrane
of which is immersed in a solution with the pH of the nominal elec-
trode zero point. The zero point is part of the offset potential.

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ORP voltage The ORP is caused by oxidizing or reducing substances dissolved in

water if these substances become effective on an electrode surface
(e. g. a gold or platinum surface).
pH value The pH is a measure of the acidic or basic effect of an aqueous so-
lution. It corresponds to the negative decadic logarithm of the molal
hydrogen ions activity divided by the unit of the molality. The practi-
cal pH value is the value of a pH measurement.
Potentiometry Name of a measuring technique. The signal (depending on the mea-
sured parameter) of the electrode is the electrical potential. The elec-
trical current remains constant.
Reset Restoring the original condition of all settings of a measuring system.
Resolution Smallest difference between two measured values that can be dis-
played by a measuring instrument.
Slope The slope of a linear calibration function.
Stability control Name for a function to check the stability of the measured value.
Standard solution The standard solution is a solution where the measured value is
known by definition. It is used to calibrate a measuring system.
Test sample Designation of the test sample ready to be measured. Normally, a
test sample is made by processing the original sample. The test
sample and original sample are identical if the test sample was not
processed.
Zero point Designation for the offset potential of a pH electrode. It is the mea-
surable potential of a symmetrical electrode, the membrane of which
is immersed in a solution with the pH of the nominal electrode zero
point (pH = 7).

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Lists ProLab 3000

Trademarks used

Trademark Owner of the trademark

Merck Merck KGaA
SCHOTT Schott Glas AG
Mettler Toledo Mettler Toledo
Fisher Fisher Scientific Company
Fluka Fluka AG
Radiometer Radiometer
Baker Mallinckrodt Baker, Inc.
Metrohm Metrohm AG
Beckman Beckman Instruments, Inc.
Hamilton Hamilton Company Corporation
Precisa Precisa Instruments AG
Reagecon Reagecon Diagnostics Limited
Riedel-de Haen Honeywell International Inc.
Windows Microsoft Corporation

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Index

A J
Authorized use .........................................21 Jack field ................................................ 15

B K
Battery compartment ..............................163 Key functions .......................................... 12
Blank value addition ...............................126 Keys ....................................................... 12

C L
Calibration Lists ..................................................... 175
ISE .................................................101
pH .....................................................72 M
Calibration evaluation
Measurement accuracy ............................ 86
ISE .................................................103
Measuring
pH .....................................................76
ISE .................................................. 96
Calibration interval ...................................86
ORP voltage ................................ 89, 91
Calibration points
pH .................................................... 69
ISE .................................................101
Measuring method ................................. 111
pH .....................................................74
Blank value addition ......................... 126
Calibration record
Sample addition ............................... 120
Stored calibration records, display .. Sample subtraction .......................... 123
136, ................................................151 Standard addition ............................. 112
Cleaning ...............................................164
Standard subtraction ........................ 117
Connecting sensors .................................15
Measuring mode
ISE .................................................. 95
D pH .................................................... 67
Data output ...................................139, 153 Menus (navigation) .................................. 41
Default settings
Measured parameter ...........................65 O
System settings ..................................65
Obligations of the purchaser ..................... 22
Delete
Operational safety ................................... 22
Measurement datasets ......................135
Display ....................................................14
Display illumination ..................................14 P
pH .......................................................... 67
F pH buffer sets ......................................... 72
Precautions ............................................ 21
Firmware update ....................................183
Principle operation ................................... 33
Print ..................................................... 155
I
Initial commissioning ................................24 R
Initialize ...................................................65
Recorder ...................................... 139, 153
Ion concentration .....................................95
Reset ..................................................... 65
ISE standard solutions ............................101
RS232 interface ............................ 154, 155
RS232 socket assignment ...................... 170

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Index ProLab 3000

S
Safe operation ........................................ 22
Safety .................................................... 21
Sample addition .................................... 120
Sample subtraction ................................ 123
Scope of delivery .................................... 23
Sensor window ....................................... 14
Single-point calibration
pH .................... 79, 80, 81, 83, 84, 107
Slope
ISE ................................................ 101
pH .................................................... 72
Stability control
Manual
ISE ............................................. 97
ORP voltage .......................... 89, 92
pH ............................................... 69
Standard addition .................................. 112
Standard addition with blank value correction
126
Standard subtraction ............................. 117
Status line .............................................. 14
Stored measurement datasets, display ....
134
Storing functions ................................... 129
System configuration
Recall ..................................... 160, 161

T
Temperature measurement ...................... 62
ISE .................................................. 95
pH .............................................. 67, 87
Temperature sensor
pH ........................................ 68, 88, 96
Temperature unit ..................................... 61
Three-point calibration
ISE ................................................ 107
pH .................................................... 80
Transmitting data .................................. 153
Transmitting measured values ................ 153
Two-point calibration
ISE ................................................ 106
pH ........................................ 79, 81, 83

Z
Zero point of the pH electrode .................. 72

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Appendix
A.18 Firmware update
General information With the "Update_ProLab3000_4000" program and a PC you can
update the firmware of the ProLab 3000 to the newest version.
Available firmware updates can be found on the Internet.
Connect the meter to a PC for the update.

For the update via the USB-B interface (USB Device), the following is
required:
z a free USB interface (virtual COM port) on your PC
z the driver for the USB interface of the PC (installation of the USB
driver from the installation CD-ROM)
z the USB cable
(Z875, included in the scope of delivery of the ProLab 3000).

For the update via the RS232 interface, the following is required:
z a free RS232 interface on your PC
z the RS232 cable, Z390.

Preparing the
8 Disconnect the ProLab 3000 from the power supply.
ProLab 3000 for the
update 9 Disconnect the interface cable (USB, RS232) from the
ProLab 3000.
10 Reconnect the power supply.
The self-test, meter designation, software version and subse-
quently the Standby display appear.

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Appendix ProLab 3000

11 Connect the ProLab 3000 to a USB interface (virtual COM port)

of the PC with the aid of the USB interface cable.
or
Connect the ProLab 3000 to a serial interface (COM port) of
the PC with the aid of the Z390 interface cable.

Program installation
1 With the installation program,
"Install_Update_ProLab3000_4000_Vx_yy_English.exe",
install the firmware update program on a PC.

Program start
2 Start the "Update_ProLab3000_4000" program from the Win-
dows start menu.
3 If necessary, change the language in the language menu.

Firmware update
4 Start the updating procedure in the firmware update program
with [OK].
5 Follow the instructions of the firmware update program.
During the programming process, a corresponding message
and a progress bar is displayed.
The programming process takes up to 15 minutes.
A terminatory message is displayed after a successful pro-
gramming process. The firmware update is completed.
6 Disconnect the ProLab 3000 from the PC.
The ProLab 3000 is ready for operation.

After switching the meter off and on you can check whether the meter
has taken over the new software version.

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A.19 Menus

A.19.1 Main menu

File File
– New configuration...
– Select configuration...
– View configuration...
– Delete configuration...
– Terminate

Memory Memory
– Manual memory
– New...
– Select...
– Display...
– Delete...

– Automatic memory
– New...
– Display...
– Delete...

– ISE method memory

– New...
– Select...
– Display...
– Delete...

– Calibration memory
– Display...
– Delete...

– Recorder
– Display...
– Delete...

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Appendix ProLab 3000

System System
– Data output...
– Baud
– Output format (ASCII, CSV)
–
– Header

– Autom. printout...
– Reset
– Measuring
– Autom. stability control
– Temperature unit
– Connect Ref1/Ref2

– Signal On
– Country and language...
– Country
– Language

– Date and time...

– Color code...
– Activate lock

Window Window
– Channels...
– Maximize recorder

User User
– Administration...
– Change password...

Help Help
– Help topics
– Meter info...
– Sensor info...
– System info...

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A.19.2 pH sensor menu

Measuring Measuring
– pH resolution
– High
– Medium
– Low

– Alternative TP
– Setting of man. temp...
– Reset pH
– Recorder...

Calibration – Calibration
– pH calibration type
– AutoCal
– Buffer sets
– VariCal
– Slope in (mV/pH / %)
– Zero point in (mV / pH)

– Calibration interval...
– Calibration record...
– Calibration history...

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Appendix ProLab 3000

A.19.3 U sensor menu

Measuring Measuring
– High resolution
– Alternative TP
– Setting of man. temp...
– Determine reference...
– Recorder...

A.19.4 dU sensor menu

Measuring Measuring
– High resolution
– Alternative TP
– Setting of man. temp...
– Referenz
– anderer U-Kanal
– Referenzwert
– Determine reference...

– Recorder...

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A.19.5 ISE sensor menu

Measuring Measuring
– ISE settings
– Units
– Ion type
– Blank value correction
– Reference measurement
– Input reference value...
– Stability control
– High
– Medium
– Low

– ISE methods
– Standard addition...
– Double standard addition...
– Standard subtraction...
– Sample addition...
– Sample subtraction...
– Blank value addition...

– Alternative TP
– Setting of man. temp...

Calibration Calibration
– Calibration record...
– Calibration history...

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190 ba75645e02 01/2010

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