Scribd
Upload
87 views15 pages

Gene Transfer in Bacteria

There are three main types of gene transfer in bacteria: 1) Conjugation - transfer of genetic material between bacterial cells through cell-to-cell contact via pilus structures. 2) Transduction - transfer of genes from one bacterium to another via bacteriophages during viral replication. 3) Transformation - uptake and integration of naked DNA from the environment into competent bacterial cells.

Uploaded by

savera savera
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPTX, PDF, TXT or read online on Scribd
87 views15 pages

Gene Transfer in Bacteria

There are three main types of gene transfer in bacteria: 1) Conjugation - transfer of genetic material between bacterial cells through cell-to-cell contact via pilus structures. 2) Transduction - transfer of genes from one bacterium to another via bacteriophages during viral replication. 3) Transformation - uptake and integration of naked DNA from the environment into competent bacterial cells.

Uploaded by

savera savera
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPTX, PDF, TXT or read online on Scribd
You are on page 1/ 15

ham

S al
L

GENE TRANSFER IN BACTERIA


Gene transfer in bacteria

• There are three types of gene transfer in bacteria by which donor DNA

is transferred into a recipient cell.

1) Conjugation Vertical gene transfer: It is


the transfer of genetic material
from parents to offspring. It may be
2) Transduction through sexual or asexual
reproduction.
Horizontal gene transfer: It is the
movement of genetic material from
3) Transformation a donor organism to a recipient
organism that is not its offspring.
1) CONJUGATION
• Bacterial conjugation is the transfer of genetic material between bacterial cells

through pili.

Steps in conjugation:
1. Donor cell produces pilus.
2. Pilus attaches to recipient cell and brings the two cells together.
3. The double stranded plasmid is converted into single strand which is then
transferred to the recipient cell.
4. Both cells synthesize a complementary strand to produce a double stranded
circular plasmid and also produce pili; both cells are now donor for the F factor.
1) CONJUGATION
• Donor cells are called F+ 

• Recipient cells are called F- 

• When F- cells receive plasmid they become F+ and then can

donate a copy of plasmid.


CONJUGATION
2) TRANSDUCTION
• Transduction is the process by which foreign DNA is introduced

into a bacterial cell by a virus.


Two cycles of virus during transduction

1) The lytic cycle: It involves the reproduction of viruses using a

host cell to manufacture more viruses; the viruses then burst out

of the cell.
Two cycles of virus during transduction
2) The lysogenic cycle: It involves the incorporation of the viral genome into the

host cell genome and it replicates along with it harmlessly. Integrated Viral DNA

is called prophage.

The lysogenic cycle does not result in immediate lysing of the host cell. When

nutrients in bacterial cells are finished, viral DNA (prophage) can become active,

reproduce and finally cause lysis of bacterial cell.


3) TRANSFORMATION
• A process by which some bacteria take up foreign genetic

material (naked DNA) from the environment.

• Bacteria that can intake naked DNA from environment are

called competent cells.

• It was first reported in Streptococcus pneumoniae by

Griffith in 1928.
TRANSFORMATION
GRIFTH EXPERIMENT ON TRANSFORMATION
GRIFTH EXPERIMENT ON TRANSFORMATION
ham
S al
L

The End

You might also like

pFad - Phonifier reborn

Pfad - The Proxy pFad of © 2024 Garber Painting. All rights reserved.

Note: This service is not intended for secure transactions such as banking, social media, email, or purchasing. Use at your own risk. We assume no liability whatsoever for broken pages.


Alternative Proxies:

Alternative Proxy

pFad Proxy

pFad v3 Proxy

pFad v4 Proxy