Oct4

Oct4
已知的结构
PDB	直系同源搜索: PDBe RCSB
PDBID列表
	1OCP、3L1P
识别号
别名	POU5F1；, OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, POU class 5 homeobox 1, Oct3/4
外部ID	OMIM：164177 MGI：101893 HomoloGene：8422 GeneCards：POU5F1
相关疾病
	史蒂芬斯－强森综合征
基因位置（人类）
染色体	6号染色体
终止	31,180,731 bp
基因位置（小鼠）
染色体	小鼠17号染色体
终止	35,821,669 bp
RNA表达模式
	; ; ; ;
	查阅更多表达数据
基因本体
分子功能	• DNA结合; • sequence-specific DNA binding; • miRNA binding; • DNA结合转录因子活性; • 转录因子结合; • transcription cis-regulatory region binding; • 血浆蛋白结合; • DNA结合转录阻遏物活性，RNA聚合酶II特异性; • DNA-binding transcription factor activity, RNA polymerase II-specific; • 泛素蛋白连接酶结合; • RNA binding;
细胞组分	• 细胞质; • 细胞质基质; • 转录调节复合物; • 核质; • 细胞核; • 线粒体;
生物学过程	• regulation of transcription, DNA-templated; • somatic stem cell population maintenance; • negative regulation of gene silencing by miRNA; • cell fate commitment involved in formation of primary germ layer; • endodermal cell fate specification; • anatomical structure morphogenesis; • mRNA transcription by RNA polymerase II; • regulation of asymmetric cell division; • negative regulation of transcription by RNA polymerase II; • transcription by RNA polymerase II; • transcription, DNA-templated; • regulation of DNA methylation-dependent heterochromatin assembly; • multicellular organism development; • cardiac cell fate determination; • response to wounding; • BMP signaling pathway involved in heart induction; • 基因调节; • regulation of heart induction by regulation of canonical Wnt signaling pathway; • blastocyst development; • positive regulation of transcription by RNA polymerase II; • positive regulation of SMAD protein signal transduction;
	Sources:Amigo / QuickGO
直系同源
	5460
	18999
ENSG00000230336; ENSG00000206454; ENSG00000204531; ENSG00000237582; ENSG00000229094;
	ENSG00000233911; ENSG00000235068
	ENSMUSG00000024406
	Q01860
	P20263
	NM_203289; NM_001173531; NM_001285986; NM_001285987; NM_002701
	NM_001252452; NM_013633
NP_001167002; NP_001272915; NP_001272916; NP_002692; NP_976034;
	NP_001272915.1
	NP_001239381; NP_038661
	维基数据
查看/编辑人类	查看/编辑小鼠

Oct4（八聚体结合转录因子4, octamer-binding transcription factor 4）,亦称为POU5F1（第五类POU结构域转录因子1），是一种蛋白质，在人体中由POU5F1基因编码^[6]。Oct4是一种具有与POU结构域（英语：POU domain）同源的结构域的转录因子。Oct4对未分化的胚胎干细胞的自我更新与干性维持至关重要^[7]。也正因如此，有时Oct4会被用来作标记未分化细胞的分子标记。Oct4的过表达或低表达都会造成细胞的分化，因此Oct4只在特定的表达强度下才能维持细胞的干性^[8]。

Oct4（八聚体结合转录因子4）名称中的“八聚体”（octamer）指DNA核苷酸序列“A TTTG CAT”^[9]。

参见

Sox2
Nanog

参考

^ 與Oct4相關的疾病；在維基數據上查看/編輯參考.
^ ^2.0 ^2.1 ^2.2 GRCh38: Ensembl release 89: ENSG00000230336、ENSG00000206454、ENSG00000204531、ENSG00000237582、ENSG00000229094、ENSG00000233911、ENSG00000235068 - Ensembl, May 2017
^ ^3.0 ^3.1 ^3.2 GRCm38: Ensembl release 89: ENSMUSG00000024406 - Ensembl, May 2017
^ Human PubMed Reference:. National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Mouse PubMed Reference:. National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Takeda J, Seino S, Bell GI. Human Oct3 gene family: cDNA sequences, alternative splicing, gene organization, chromosomal location, and expression at low levels in adult tissues. Nucleic Acids Research. Sep 1992, 20 (17): 4613–20. PMC 334192 . PMID 1408763. doi:10.1093/nar/20.17.4613.
^ Young Lab- Core Transcriptional Regulatory Circuitry in Human Embryonic Stem Cells （页面存档备份，存于互联网档案馆） at MIT
^ Niwa H, Miyazaki J, Smith AG. Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells. Nature Genetics. Apr 2000, 24 (4): 372–6. PMID 10742100. doi:10.1038/74199.
^ Petryniak B, Staudt LM, Postema CE, McCormack WT, Thompson CB. Characterization of chicken octamer-binding proteins demonstrates that POU domain-containing homeobox transcription factors have been highly conserved during vertebrate evolution. Proceedings of the National Academy of Sciences of the United States of America. Feb 1990, 87 (3): 1099–103. PMC 53418 . PMID 1967834. doi:10.1073/pnas.87.3.1099.

拓展阅读

Lamoury FM, Croitoru-Lamoury J, Brew BJ. Undifferentiated mouse mesenchymal stem cells spontaneously express neural and stem cell markers Oct-4 and Rex-1. Cytotherapy. 2006, 8 (3): 228–42. PMID 16793732. doi:10.1080/14653240600735875.
Hough SR, Clements I, Welch PJ, Wiederholt KA. Differentiation of mouse embryonic stem cells after RNA interference-mediated silencing of OCT4 and Nanog. Stem Cells. Jun 2006, 24 (6): 1467–75. PMID 16456133. doi:10.1634/stemcells.2005-0475.
Feldman N, Gerson A, Fang J, Li E, Zhang Y, Shinkai Y, Cedar H, Bergman Y. G9a-mediated irreversible epigenetic inactivation of Oct-3/4 during early embryogenesis. Nature Cell Biology. Feb 2006, 8 (2): 188–94. PMID 16415856. doi:10.1038/ncb1353.
Boyer LA, Lee TI, Cole MF, Johnstone SE, Levine SS, Zucker JP, Guenther MG, Kumar RM, Murray HL, Jenner RG, Gifford DK, Melton DA, Jaenisch R, Young RA. Core transcriptional regulatory circuitry in human embryonic stem cells. Cell. Sep 2005, 122 (6): 947–56. PMC 3006442 . PMID 16153702. doi:10.1016/j.cell.2005.08.020.
Gerrard L, Zhao D, Clark AJ, Cui W. Stably transfected human embryonic stem cell clones express OCT4-specific green fluorescent protein and maintain self-renewal and pluripotency. Stem Cells. 2005, 23 (1): 124–33. PMID 15625129. doi:10.1634/stemcells.2004-0102.
Reményi A, Lins K, Nissen LJ, Reinbold R, Schöler HR, Wilmanns M. Crystal structure of a POU/HMG/DNA ternary complex suggests differential assembly of Oct4 and Sox2 on two enhancers. Genes & Development. Aug 2003, 17 (16): 2048–59. PMC 196258 . PMID 12923055. doi:10.1101/gad.269303.
Schoorlemmer J, Kruijer W. Octamer-dependent regulation of the kFGF gene in embryonal carcinoma and embryonic stem cells. Mechanisms of Development. Dec 1991, 36 (1-2): 75–86. PMID 1723621. doi:10.1016/0925-4773(91)90074-G.
Wey E, Lyons GE, Schäfer BW. A human POU domain gene, mPOU, is expressed in developing brain and specific adult tissues. European Journal of Biochemistry / FEBS. Mar 1994, 220 (3): 753–62. PMID 7908264. doi:10.1111/j.1432-1033.1994.tb18676.x.
Crouau-Roy B, Amadou C, Bouissou C, Clayton J, Vernet C, Ribouchon MT, Pontarotti P. Localization of the OTF3 gene within the human MHC class I region by physical and meiotic mapping. Genomics. May 1994, 21 (1): 241–3. PMID 8088794. doi:10.1006/geno.1994.1249.
Guillaudeux T, Mattei MG, Depetris D, Le Bouteiller P, Pontarotti P. In situ hybridization localizes the human OTF3 to chromosome 6p21.3-->p22 and OTF3L to 12p13. Cytogenetics and Cell Genetics. 1993, 63 (4): 212–4. PMID 8500351. doi:10.1159/000133537.
Abdel-Rahman B, Fiddler M, Rappolee D, Pergament E. Expression of transcription regulating genes in human preimplantation embryos. Human Reproduction. Oct 1995, 10 (10): 2787–92. PMID 8567814.
Hillier LD, Lennon G, Becker M, Bonaldo MF, Chiapelli B, Chissoe S, Dietrich N, DuBuque T, Favello A, Gish W, Hawkins M, Hultman M, Kucaba T, Lacy M, Le M, Le N, Mardis E, Moore B, Morris M, Parsons J, Prange C, Rifkin L, Rohlfing T, Schellenberg K, Bento Soares M, Tan F, Thierry-Meg J, Trevaskis E, Underwood K, Wohldman P, Waterston R, Wilson R, Marra M. Generation and analysis of 280,000 human expressed sequence tags. Genome Research. Sep 1996, 6 (9): 807–28. PMID 8889549. doi:10.1101/gr.6.9.807.
Inamoto S, Segil N, Pan ZQ, Kimura M, Roeder RG. The cyclin-dependent kinase-activating kinase (CAK) assembly factor, MAT1, targets and enhances CAK activity on the POU domains of octamer transcription factors. The Journal of Biological Chemistry. Nov 1997, 272 (47): 29852–8. PMID 9368058. doi:10.1074/jbc.272.47.29852.
Nichols J, Zevnik B, Anastassiadis K, Niwa H, Klewe-Nebenius D, Chambers I, Schöler H, Smith A. Formation of pluripotent stem cells in the mammalian embryo depends on the POU transcription factor Oct4. Cell. Oct 1998, 95 (3): 379–91. PMID 9814708. doi:10.1016/S0092-8674(00)81769-9.
Gonzalez MI, Robins DM. Oct-1 preferentially interacts with androgen receptor in a DNA-dependent manner that facilitates recruitment of SRC-1. The Journal of Biological Chemistry. Mar 2001, 276 (9): 6420–8. PMID 11096094. doi:10.1074/jbc.M008689200.
Butteroni C, De Felici M, Schöler HR, Pesce M. Phage display screening reveals an association between germline-specific transcription factor Oct-4 and multiple cellular proteins. Journal of Molecular Biology. Dec 2000, 304 (4): 529–40. PMID 11099378. doi:10.1006/jmbi.2000.4238.
Ezashi T, Ghosh D, Roberts RM. Repression of Ets-2-induced transactivation of the tau interferon promoter by Oct-4. Molecular and Cellular Biology. Dec 2001, 21 (23): 7883–91. PMC 99954 . PMID 11689681. doi:10.1128/MCB.21.23.7883-7891.2001.
Guo Y, Costa R, Ramsey H, Starnes T, Vance G, Robertson K, Kelley M, Reinbold R, Scholer H, Hromas R. The embryonic stem cell transcription factors Oct-4 and FoxD3 interact to regulate endodermal-specific promoter expression. Proceedings of the National Academy of Sciences of the United States of America. Mar 2002, 99 (6): 3663–7. PMC 122580 . PMID 11891324. doi:10.1073/pnas.062041099.
Looijenga LH, Stoop H, de Leeuw HP, de Gouveia Brazao CA, Gillis AJ, van Roozendaal KE, van Zoelen EJ, Weber RF, Wolffenbuttel KP, van Dekken H, Honecker F, Bokemeyer C, Perlman EJ, Schneider DT, Kononen J, Sauter G, Oosterhuis JW. POU5F1 (OCT3/4) identifies cells with pluripotent potential in human germ cell tumors. Cancer Research. May 2003, 63 (9): 2244–50. PMID 12727846.
Wang P, Branch DR, Bali M, Schultz GA, Goss PE, Jin T. The POU homeodomain protein OCT3 as a potential transcriptional activator for fibroblast growth factor-4 (FGF-4) in human breast cancer cells. The Biochemical Journal. Oct 2003, 375 (Pt 1): 199–205. PMC 1223663 . PMID 12841847. doi:10.1042/BJ20030579.
Reményi A, Lins K, Nissen LJ, Reinbold R, Schöler HR, Wilmanns M. Crystal structure of a POU/HMG/DNA ternary complex suggests differential assembly of Oct4 and Sox2 on two enhancers. Genes & Development. Aug 2003, 17 (16): 2048–59. PMC 196258 . PMID 12923055. doi:10.1101/gad.269303.
Rajpert-De Meyts E, Hanstein R, Jørgensen N, Graem N, Vogt PH, Skakkebaek NE. Developmental expression of POU5F1 (OCT-3/4) in normal and dysgenetic human gonads. Human Reproduction. Jun 2004, 19 (6): 1338–44. PMID 15105401. doi:10.1093/humrep/deh265.
Matin MM, Walsh JR, Gokhale PJ, Draper JS, Bahrami AR, Morton I, Moore HD, Andrews PW. Specific knockdown of Oct4 and beta2-microglobulin expression by RNA interference in human embryonic stem cells and embryonic carcinoma cells. Stem Cells. 2005, 22 (5): 659–68. PMID 15342930. doi:10.1634/stemcells.22-5-659.
Baal N, Reisinger K, Jahr H, Bohle RM, Liang O, Münstedt K, Rao CV, Preissner KT, Zygmunt MT. Expression of transcription factor Oct-4 and other embryonic genes in CD133 positive cells from human umbilical cord blood. Thrombosis and Haemostasis. Oct 2004, 92 (4): 767–75. PMID 15467907. doi:10.1160/TH04-02-0079.

外部链接

[1] 與Oct4相關的疾病；在維基數據上查看/編輯參考.

[refGRCh38Ensembl-2] 2.0 ^2.1 ^2.2 GRCh38: Ensembl release 89: ENSG00000230336、ENSG00000206454、ENSG00000204531、ENSG00000237582、ENSG00000229094、ENSG00000233911、ENSG00000235068 - Ensembl, May 2017

[refGRCm38Ensembl-3] 3.0 ^3.1 ^3.2 GRCm38: Ensembl release 89: ENSMUSG00000024406 - Ensembl, May 2017

[4] Human PubMed Reference:. National Center for Biotechnology Information, U.S. National Library of Medicine.

[5] Mouse PubMed Reference:. National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid1408763-6] Takeda J, Seino S, Bell GI. Human Oct3 gene family: cDNA sequences, alternative splicing, gene organization, chromosomal location, and expression at low levels in adult tissues. Nucleic Acids Research. Sep 1992, 20 (17): 4613–20. PMC 334192 . PMID 1408763. doi:10.1093/nar/20.17.4613.

[7] Young Lab- Core Transcriptional Regulatory Circuitry in Human Embryonic Stem Cells （页面存档备份，存于互联网档案馆） at MIT

[pmid10742100-8] Niwa H, Miyazaki J, Smith AG. Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells. Nature Genetics. Apr 2000, 24 (4): 372–6. PMID 10742100. doi:10.1038/74199.

[9] Petryniak B, Staudt LM, Postema CE, McCormack WT, Thompson CB. Characterization of chicken octamer-binding proteins demonstrates that POU domain-containing homeobox transcription factors have been highly conserved during vertebrate evolution. Proceedings of the National Academy of Sciences of the United States of America. Feb 1990, 87 (3): 1099–103. PMC 53418 . PMID 1967834. doi:10.1073/pnas.87.3.1099.

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参见

参考

拓展阅读

外部链接

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