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 Zone Size lnterpretative GhartY
Sesi-
slanl
ATCC
mm
700603
0l
less

fnterobaclenaceae p aerugioosa,
AcientaDactet I Stapry/ococcus spp r5 16 14

. Amoryclav
(Amoricillif,/Clavutanic acid)
Entercbacteriaceae 4-17
1 13
Haenophilus influenzae &
Haemo ph tIu s p arail I Io e a za e r9
'19

16
t)
17-23
19
15

EnlerobacteMceae
Staphylococcus spp.
Enlerccoccus spp. -tl
_l-l
Haenophilus inlluenzae &
H ae m op hiI u s para i nl I ue ua e
Slrepaccoccus Spp. beta
haemol!,ttc group
-i-l30-36
Enlercbaaeftles
Staplrylo c oc c u s s a p r op h yti c u s
Eillerccoccus spp.
St@toccccus spp. viridails groltp
Haenophtlus kffuenzae &
Listeia nonacytogens
Pasteuela tnuttocida'
Aetococcus nnguinicola & urinae

Staphylococ cus, S. p ne u ma nj ae,

) otEptuouLLur
Slreplococcus rpg_ vntuut$ groul
spp. Vridians lroup &
i Slreplococcus spp. bela
haeorclytic group
i Haenophih$ inltuen2ae &
-t 1 9-25
H ae n aphl rs p ar at n I I u e n zae

)il. i_ i_ _t
_l 1 3-21

10-16

EntercbacteriaceagStaphytocaccusspp. 1B iS i, i.i

_t

y: lnaccordancetoPerformancestandardsforAntimicrobialDisksusceptibilityTests,cLSl&EUcAsT.
I* : Zone size inlerprelative criteria given in Bed colour is as per EtlCASTslandard (The Eur0pean Commitiee 0n Antilnrcr0biat Susceptibilily Testing)
: Not included in cLSI charti performance standards for Antimicrobiai Discs obtained from drug manufaclurers.
. : 0n receipt, store at -20'C. FDA approved
0n receipt all other products to be stored between -20'c t0 g'c. For prolonged use, store at 0r below -20.c.
2 FotE.coli,s.aureus,P.aerugin1sa: MuellerHintonAgar([rHA). FotHaenophilusspp.;HaemophilusTestMedium;
For S. pneunoniae:Mueller Hinton Agar with 5% sheep blood; tot N. gonorrhoeae:cb
Agar Base with 1% defined gr;Mh supptement.
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 Zone $ize lnterpretatiue ShartY
lnterprelative Criteria
Qualily Conlrol Limits (mm)
Product Antimicrobial sym- lisc lnier
Code Agenl
Sen" Re$i- E. col) . auteus o,
eet!gifrosa E. coli S, aurers t laecalis A, I a-:- a
irtluenrae 2a fr gonothoeae :. jeiuni
bol :oniEnl silive mediale stanl Afcc ATCC ATCC ATCC ATCC ATCC ATCC ATCC I-:: ATCC ATCC ATCC
mm
0t
mm mm
0r
25922 25923 2i853 35218 292r 3 29212 49247 43766 -::i": 49226 4961S 33560
m0re less

sD062 i ceftazidime CAZ 30 mcg

Enterobactehaceae, B. cepacia 21 16-20 1l 25-,32
?aeruginosa, Acientobactet il 10-18
SlaF,hylarccc)us spp 18 15-1 7
Haemophilus intlLteiae d
14 16-20 -- 29

H [1e n a p h i i u s p ara nl i u e l z ae 26
Neisseria gonathaeae
27 35
31 l
35-43
o Cellaridine CAZ 10 mcg
!:aierat)aiereles 22 lo. r1
Ps"Drdclrrra.s s!D 6-12
17 .27
sD110 . Celtizorime ca 30 mcg
tnterobacteriaceae 25 22-24 21 30 27-35
36 12-17
Haeinaphilus irfluenzae & 28-34
ij a a n i a p h iIu s pa i an il u e nzae 2b
Neisseria ganarhoeae 33
s0065 . Ceflriaxone CTR 30 mcg
Enterobacteriaceae 20-22 19
Pae ru g i n osa, Acie nlo bacter &
16-24
Staphylococcus spp. 21 14-20 '13 ],",, 22-28
H aeno ph ilu s infIue nzae &
H ae no ph i Iu s parai nfI u enzae 26 l
31-39
N ei sse ria
Neisseria gonorhaeae
n e ni n g iti d i s

Streptococcus spp. Viridians group

34
35
27 iu-ru 2'1
L 39 51

Slrepto6occus spp. ,0,-.

beta haemolltia group 24 I

tnlerobacterales 25 22.?A
Si/epiocorcrs spo viridans qror! rclzz
2i Izs;s
Haencphilus iniluenzae 3l
f,l c i axe I Ia c ai a ffha Ii \ 24 2_1,23
r,ingeila kinqae 3C

s006'l . Celurcxime CXi\4 30 mcg

Enterahact€ riace ae lparcfienl) &
Slaprr'/ococcrs spp. 15-1 7 i4 20-26 27-35
Enterahaclelaaeie t.ia \ 23 15-22 14
Haencpliius i.ilit,"tae &
il a e na p h i tu s p a r ai nl I u e nzae 20 l7 t9 t5
Neisseria gancihoeae 31 26 30 25
t,ru

sD050 . Cephalolhin CEP 30 ncg

s0006 Chloramphenicol C 30 mc!
Ente i
rabacte ac eae, Staphyl 0c o tcu s &
Enterococcus spp. 13,17
18 12 21 -2i 1 9-26
Haenophilus influenzae &
H ae mophi Ius p arairl I u enzae 29 26 2,1 25
N ei sse ria ne n i ng ilid is 26 13
S. pneunoniae 21 20
23-27
Sfepf0coccus spp. Viridians group &
I
Sfreplococcus spp.
beta haemolJ,,tic group 2i 18-2tl I
iilerabacterales 21 -27
Siapnylacoccus s,0p
20-28
S//eplococors groupA B, C & c
S. pneunoniae
HaenoDhilus influenzae
llaiuella caiatrhalis
s0245 Cinoxacin clN 1C0 mcg
Entercbacteriaceae t9 14 2b 3i
s0060 Ciprolioxacin CiP mcg
Entercbacteiiaceae alhet that S. Typhl
& extrainiestinal Skrrore//a spp..
P a e rLq I r, a sa Ac;anto"hacte(
I I

-iiiparlca!;tis i t/r:€ii;iai-! ;tp 21 1il20 15 22-30 25-33

For S Iyphl anc €iirarf leslif a
Sa/monEra sop 31 21 30 2A
llaenaDhilus iailuenzae &
Haen aphi i us perainiluenzae 21
I'leisse ria ne ni xg ildis 35 33-34
Neisseta lanorhaeae 41 2B-40

Y: lnaccordancetoPerformanceStandardsforAntimicr0bialoiskSusceptibilityTests,CLSl&EUCAST.
I : z0ne size interpretaiive criteria qiuen in Hed e0l0ur is as per EiJCAST standard (The European Committee 0n Antimicrobial Susceptibitity Tesling)-
" : Noi included in CLSI chart; FDA approved perlormafice standards f0r Antjmicrobial Discs obtained from druo manuiaciurers.
. : 0n recerpt. store at -20"C.
0n receipl ali 0thei pro6x61, to O.,,ored betweer -20'C to 8"C. F0r pr0l0nged uSe. Slore at 0r be,0r/ -2:,C.
A"
-t lor E. coli S aureils. P. aetuginosa: N4ueiler Hinton Agar :MHA). Fot Haemophiius $',op. : qaem0phrlus resr Med,um,
ForS.pneunorlre:MuellerHintonAgarwilh5%sheepblood;ForM g1norrhoeae:GCAgarBasewilhlgbdeflnedgi0Mhsuppiement. EFTI A
L',,IFI
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Zone Size lnterpretative ChartY
lnlerpre!alive Crileria 0uality Conlrol Lim'ts (mm)
Producl Anlimicrobial sym- Dasc sen- nler- ilesi- E. coli l- ailrcul E. coli - aurcus E. '1. a : : :,
iillluenzae tr- \. gaailhcezt neunonii
Code Agenl bol Donleni silive nediale slanl ATCC ATCC ATCC ATCC ATCC ATCC ATCC ATCC :-:: AtCt ATCC ATCC
mm nm mm 25922 25S23 27853 29213 29212 45247 49766 -::::: 49226 49619 33560
0r 0t
m0re less

Erylhrcmycin
:',jrltytn't).t:t); :, F,tit:t.)f0C1 |\ !!!
! rlri-,rr,,lr:,4 -!//epidatICr/ri :Qp
l/nd t3r S .l. r: A qlrpr/ratf t!:i !ll!
i

-lfta 'taaltr fi r riri rll

Galilloxacin
iace ae, P ae rug i nos a,
E nl erobacte
Acientobactet & Enterococcus spp
Slaprylococcus spp.
llaenrc p h ilu s i nf I u enzae &
Hae mo ph ilu s parail iu e nz ae
Neissetia gonothoeae
S. pneunoniae, Slreplococcus spp.
Vkidians group, Streptacoccus spp.
beta haemolylic group 24-31

Heemophilus inlluenzae &

Haeno ph ilus pa rainf lu e nz ae
S. pneumoniae

| ,"t )l,atq,"! rf ! aL,riqriis,t

Aai,t loblt\" &''lllr/hrrr'ar/,: .X!

. lmipef,em
inlerch?clPtiTeac
P aen)0inosc
Haenrphilt)s irl!tr/re,1
haen)a, tio s Drt! nl itet rxe
11
21-29
/cier/obaclcl spl]

2+30
|
-l

E ntero b acte tiic e ae, S Iy pl1

P aeruginosa, Acienlobacler spp.,
S. mallophila, Enterocaccus sag.,
S pneuqiontac-

y !r rriror!.ilra."l0PeiirririfT51;l,rar.d5lcrA.r,:- r:biil0skSrrsaEpilbrityTustsl.lLS d

- l.j.rlirtlrr(l,r{i rlLS rhad LLlAappr0vedperl0frnancislandardsl0rAntlmrcr0balDscscLhtaiIed1'] : -r'.riLiactuIers.

. r il: r.aa !t -s10rr. rt '20' i
0irr.!Litii cthrr0r0rjrclslr.!nitllrcilfrEt'r.'cri,-20"C103'C FOrprclcrOeduse slai,rit:rbt: -, ,
F!r | ,rrr,. S eLr:!ts P eetLn)tt0::2 Iilrel er llrnlon Agar tl\,4HA\ Fct Haetnolthtltls spp : Haem0Dlr[]s ra.' r.':!!um:
Ft, s a.r:,/r'!iir. L,lLrr i"r ll rifr Aqar Hith 5'. sireet blc00 F,I /y' Enn1rilueat . GC Arar Brs€,r. th 1' :'- -,ar !r0yrth suppiefleri utn,iErrl ir
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 Zone Size lnterpretative GhartY
Int8rpretatiys Criteria
ouality Conkol Limits {mmt
P,oducl Aolimicrcbial sym' 0isc Sen- lnlcr Sesi E. coli i- aweul P aerugioosi E. col) S. aurers E. laecalis inllueotae
Co& ACsrt ll. gonoilhoeat jeiuni
bol :0nleil sltive mediate slanl Afcc ATCC ATCC ATCC ATCC ATCC AICC Aicc
C.
ATCC ATCC ATCC ATCC
mm mm nm 25922 25923 27853 29213 29212 49247 1!t 66 700603 49226 j96r I 33560
0r 0r
MOIG less

s0021 Nalidixic Acid NA ]0 mcg

Enterobacleriaceae 19 l4-1 I 13 22 28
2

s0046 t{etillin (iletilmicin $ulphate) NET JU mcg

fntercbacteriaceae, P aeruginosa,
Staphylococcus spp. t5 13-14 12 22-3C l- l
s0085 NE] 10 mcg
15 2-14 12 18-24
18 18 20.26
22 22
12 12
16
s0023 Nitrolurantoin NIT 300 mcg
Enterobacteriaceae Staphylocaccus &
Enterococcus spp. 17 15-16 14 20-21 18 22
23-29
SDU8fi t{lholurantoin NIT 100 mcg
Enterobactenles '1'l
17-23
Slaprylococcus spp. 13 IJ
Slreplococcrs group,4. B, C & G, 15 15
E lerococcus spp. '15
15
Aercc oc cus sang uinDo Ia & u ri nae 16 16
i0196 t{ilruoline NO l0 m(
Enlerobactenlcs
(uncomplicated UIls only) 15 15 18-24
s0057 NX 10 mcg
Enle robact cti ac eae. P ae ru g in o s a,

Staphylococcus & Enlerocaccus spp. 17 13-16 12 17 -28 22-29

28-35

,u-r,
12

.s0053 ilovobiocin NV 30 mcg 17 18-21 22 22-31

s0087 ofloracin OF 5 mcg
tntercbacteriaceae, P aeruginasa,
S. pneunoniae, Steptococcus spp.
Viidians group, Streptococcus spp
bela haemolytic group r6 13-15 12 29-33 17-21
Slaphylococcus spp. ]B 't5-17 14 24-28
Haenophilus inlluenzae &
Haemophilus parainlluenzae 16
31-40
Neisseila gonorhoeae 31 25-30 24
43-51

s0088 .oracillin OX 1 mcg

Slaphy lo cocc u s lS. p se ud it e rn e di us) 18 17 18-24
S. pneumooiae 20

s0070 Polloracin PF. mcg

tntercbactenaceae I.S lypll ) 24 23 I
26-32 I

s0028 . PenicilliFc P l0 units

Staphylococcus spp. 29 28 26-37
Enterccoccus spp. 15 14
Neisseria gonothoeae 47 iluu 26
Streplococcus sDp.
beta haemolytic group

I
24
s0089 a

12 I
6-9
I
15-_21
IJ

29

25 I

s0066 . Piperacillin 100 mcA

Enlerobactetiaceae & Acientobacter spp. ai 18 20 17
P aeruginosa l 1 5-20 14 ,un
SD066A PI JU mcg
Enterobacterales 20 17-19 17 21-2
Pseudomoms spp 18 18 I

y : lnacc0rdancetoPeTfofluanceSlafda(JslcrAntrmcfobialDiskSirsce!lb rt!-iisl3 CLS &

' : N0tincludedlnCLSlchart:FDAappr0vedperfoimancestandardsf0rAntimicr0ora Dlscs0btainedfr:- I t-ranillacturers.

a./ : DeterioratJoninoxacllindlsccontertrsbestassessedwith0C0rqanismsa!/eusATCC25923r,:-:-,:::::biezcnrd:an?lar0f1g-24mfi.
. : 0n recetpt. store at -20'C
8 0n receipt all cther pr0du,rts t0 be st0red bet\,reen 20'c ic 8'c. F0r pr0i0,rqed rs€. :taie !t 0r tle i.. ,_,
to( E coli. s aureus. P. aerug'fi{)sa: lvlueller llini0n Aaar (MHA). tai Haeorcph //rs spp. : Ha€m0philLrs r-:.: ,.,:r
FatS.pneunoriae l\lu€lerHlnl0nAqarlvllh5l"sheepblood:FatN.ganarrhaeae:GCAgarBasewlthl::
--.
i'itl.tJ;ihsup0rement MEEEI'
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Zone Size lnterpretatiue GhartY
lnlenretative Criteria ouality Cofltrol Limits (nm)
,rodoct Ailimicrobial sym- 0isc len- ntoF Resi- E. coli i. auteul E. coli S. aofeus E. laecefis k. pneunoniae gonorhoeae C. iejuni
:ode Aqent bol ]ontent iilive nediate slanl Afcc ATCC ATCC ATCC ATCC ATCC AICC ATCC ATCC Arcc ATCC
nm nm mm 25922 25923 21853 352't8 29213 15241 4S766 700603 49226 ::-. i 33560
,t 0r
n0re ess

'10 mcg
s0044 Tobramycin TOB
Enlercbaclet'aceae P aeiognasa.
Acieplobcte, & Slapttilaaa.ils spp l lr-rl r,r ii r 9-29

I
I

s0039 Trimelhoprim TB 5 mcg

L nl e r a b a cler ia c ea e, S la prylococcus spf l,j |15 11) 21-28 I 9-26
?1 28
2z-:8
24-32

s0268 t lilloracin (Prulilloracin) PBU mcg

s0045 Vancomycin l0 mcg 17-21

frlerococcus spp. it ir-, u ,o
S. pneunofiae, Streplacoccus spp.
beta haemolytic group &
Strcptococcus spp. Viridians group 17 20 27
s0155 Yammrcb mc0
Enlertrmrus spp. 12 12 10-16
Slreplococcus oroup A, B, C & G 13 13
S:Wumoniae 16 16 17-23
Sfeplocmcus spp. vlridans qroup 15
Cuynebackiun spp. 17 17
/a,1mcul sauuinicola & uinae 16 16

v lf a,:aDrlancc t0 Pcrfornrirnar Sti r,1aril., i! .\nr,ri|Lrobra D sl. S!:!tnl ir rl,l Tfst ijL: ,\

' 0n rece pt, slrrc ai -llo'i)

0nreceplalotherprodtclst0l)estoredbcti'ran.2C'Ci08(l l!ri)rr,i,,liFrllrse.si,reiir,rilr,:),.,,'lli,
ForE L,r, S treLts P st:ru?trtitst fJrelrrf r:'rf A, i'ir'l "r '' r:,,i, llir 1,1 ir - . '.'
iol l.pttcutttottrac. iviueiu nrtLuL AVdr ilrli ! or drpJruuu.ru, ! yr r,, "Ldc u!Aqn, Drsi\ ri I

Zone size interpretative chart for Antifungal agent

(Based on results obtained on Mueller Hinton Agar + 2% Glucose + Methylene Blue Dye Medium)
diamcter, (mm)
pt0a€ l- c.rAicars ]l c. paapsitosd-lf cnpicatts
morc ii rrccgoozo ll ATcczz0rg ll rrcczso
25 mcg 28-39
1 mcg

" S-00 ' Suscepi ble - D0se iiet,.ir.renl

Belereraas I 1,1!1t-.a'0flrllL.qa rr"ii1-:!-S-sa:::l:--:a'': ':'a:::: ifLrrcve,,lG!deln--r':. -: . .- - :.-.,:,,: : - '.'-.::
For more detarrs rcleT lo 1lIs volume

lcsin,j0i\?a:ts TlrLrdlni0Trltalr0fillSi[rr']ne,'tCLr:1.,r: r 1.':r l' r.,r200C

10
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 0u ality Gontrol Limits tu: Antibiotics
Bas pd nn Results obtained ur l\/lr rollor l-lintnr
niamerer oi zomoi intiitilionlmm Diameter 0f zone ot inhibilion in mm

0ual ts
E. coli S. aureus \ aetugtnosa E. coli \. aureus P aeruginosa
Product tnlimicrobiat 3yril. Disc ATCC ATCC ATCC PrDducl qniiifiicrobial Bym" 0iso ATCC AICC ATCC
Dode qgenl lol lonteni 25922 25923 27853 ijode {qenl 3ol !'onlent 25922 25923 27853

s0cll8 Iriple Sulpha S3 i00 mcq SD163 /ancomvcin 0 mcq 17-21

SDl 99 Iviosine IL i mcn 22-30 SD,182 /ir0inamVCin 'l 5 mco 22-30

and results are recorded cn the basis cf appearance 0f growth usinq Cl. per{ringens (ATCC 1292ji as a test r,rg,rn sm

Product Code Antimicrobial Agenl Symbol Irisc conlenl F orffi --]l- o ,--][. r-=lf3 -]f s --][-7 -l
bUUZU

s0099
l\4etronidazole

l\4etronidazole
t\,17

IV]T
5 mcg

4 mcg
E$ .=l!-,,,,* lt
" addition ro 5 ml medium
s'.d ]t e*hfi{[r f;* --]

Z{}NE SIUE CHAffiT

'N'TEMPffiHTATIVf; lnterpretatiye criteria
-
Antimicrobial I |
Producl Symbol Disc I +--
Code Agenl conient i - Sensitive j nesistant ' Bactercides lragitisAToo 25285 Fusobactetiun neuophorun A1CC zi?86
I ,
iso837 [Kanamf I r ] tooomlg_]-tlImm
'*:Expecieddiameteroizoneoliflhlbltoil,asperQualityControlresullsobtainedonBrucelaBoodA-0arwHemirandVtamrnKl
L ___-_ _
livl1039).
-
Ouality Control I tor Antifr gal Agenl

tr-l
] c(lnreii I
C.albicans
ATCC
9763
8-12 10-18
8-1 0
-of
Amphotericin-B 16-25
17-25

18-22 18"22
20"32 14.29 18-22
30 mcg 26-32
s0274 Xeloconazole 50 mcg W 3644

I
sD273 i
Miconazole
lqryq 22-26 13-17
i sozzz i Miconazole 50 mcg 26-32 23.29 2C 2,
l[,]1,, i 6-25
I Nystatin 50 mcg 1 9-23 22-2-;

6. All Slaphr/0.0c.a/ is0lates with zone diameters of '14 mm or less should be tested by an
tu]lC method.
These discs are used for semi-quantitativB in yfro susceptibility testlng by the agar disc 0iffusi0f
tesi. The vaiues obtained by this test can be c0nsidered as a guide t0 therapy selecti0n only after 7.WhentestinqVancomycinagainstE lerococci,platesshouldbeheldaflill24hrs&examined
taking into c0nsiderati0n several other fact0rs; and must be the sole decisron and resp0nsibtlity using transmtted lght; the presence 0f a haze or any grornth within the zone 0l inhibiti0n
ind cates rcsistrncg.
of the physlcian al0ng with the clinical experience in treating the infectlon. These tests are
c0mparable to the standards as per the given specifications and set 0f expeflment standards as 8. Ofloxacin susceptrble S. pneumoniae will also be susceptible to Levofloxacin.
far as p0ssible. Please refer to CLSI & EUCAST standafds f0r detailed iim tatlon of susceptibllity L Susceptibiiity & resistance t0 Azithr0myc n. Clarilhr0i-n,/cin & Dirithr0mycin can be predicted
test cir the cl iical use 0l an antibiotrc in vanous tlterapeutic c0nd tions. [''l ils ng Erythro-yc. fo" StrFp:Jc0.-a

L Anipiclilin disc is useo for testing susceptibillty t0 Amoxycrllin as vre . 1.Ba;e'I ,', l,-:)','l'.1 5rerr;J.C.&Trrckl,i 1966 Am.J.Clin.Patho.,45:493.
2. Cephai0thrn Cis{r is used for'testing susceptibility to Cephapirin, Cephaloridine, Cephalexin, 2. Bryskier A., Antimicrobial agents. 2005, ASM Press, USA.
Celac!0', Ceploxitirr Cefazo rr, Cephrad ne ald Ce0haCrOxil as \yeii 3. Ci rlaal & Lab0r':ricry Siarda,{ls llstllrte Perfo.na'r.e Sl?nd?rds ror An+iT r.0bi?l
3. Tne Su { soxazole (Suiphaflrrazc el disc can be used t0 represen'i any 0fthe c!re.i v ara latr e SlscictibiiU resric f.llaiOS 23:^ E: :L: ,: - 3. ,a -.--:,-5 l:S ,',.,-: PA
sulionamrde preparations. ] 9:3. USA,

4. The category "lntermediate" sh0uld be reported. Infecti0ns r\,ith bacteria of ntermediale -: :enberg, H.D. Clinical [4icrobioogy Procedures Handbook.2nd Editon, Vol.'1, Section 2.
susceptibility may be considered nr0derately susceptlble and may respond to antincr..,al a :.rlerg, H.D. Clinicai Microbiology Procedures Handbook. 2nd Edition, Vol. 3, Section 1 5.
agents with a wide sale dosage range. 5.;196nsen.J.H.,Ptaller,M.A.,Carr0ll,K.C.,Funke,G.,Landry,M.L., RichteiS.SandWarnock.,
5. Since certain strains oI Providencia spp. have been reported t0 give false suscepiible r.:- 1s D ,', 201 5) lvlanual 0f Clinical t!4icrobi0l0gy, 1 1 th Edition. V0l. 1 .
with Cefprozil discs, strains of this genus should not be tested & reported with this d s. ;:,.'a:\./.,AntiDioticsif Laboratoryrnedicine.2005,LippincottWilliams&Wilkins,USA.
t :::itsh G. F., 1929, J. Lab. Clin. Med., 14:649.

: Concentrat0n 0iAntib Otics notas perCl-Sl & nol as per EUCAST.

LEqFEtl cEpartner4u, FoB tN vlrRo ottclosrtcs

(€ ESDOOHNLAAN 13,
lrvo=r

3951D8 MAARN, NL HiMedia Laboratories Pvt. Limited

23, Vadhani lndustrial Estate, LBS Marg, Mumbai -400 086, MS, lndia

12
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