Instructions for use

CO2 INCUBATOR

CO2Cell 190 Comfort

CO2Cell 190S Comfort
(with sterilization 200 °C)

CO2CELL Comfort 190_np_en 1301_mmm_V1.02

CONTENTS:

24. 1. 2013

1 SAFETY ......................................................................................................................................................3
1.1 LEGAL CONSIDERATIONS ............................................................................................................................ 3
1.2 INTENDED USE .........................................................................................................................................3
1.3 SYMBOLS USED IN THIS MANUAL.................................................................................................................. 3
1.4 GENERAL SAFETY INSTRUCTIONS ................................................................................................................. 3
2 DESCRIPTION .............................................................................................................................................3
2.1 CONSTRUCTION .......................................................................................................................................4
2.2 DOORS ..................................................................................................................................................4
2.3 CONTROL SYSTEM ....................................................................................................................................4
2.4 CO2 SENSOR ........................................................................................................................................... 4
2.5 DIAGRAMS ..............................................................................................................................................4
2.5.1 OUTER VIEW ...................................................................................................................................... 4
2.5.2 INNER VIEW ....................................................................................................................................... 4
2.5.3 REAR CONNECTIONS ............................................................................................................................ 4

3 DELIVERY, TRANSPORTATION AND STORAGE ...................................................................................... 5

3.1 UNPACKING .............................................................................................................................................5
3.2 LIFTING AND MOVING ................................................................................................................................ 5
3.3 STORAGE ...............................................................................................................................................5

4 INSTALLATION ............................................................................................................................................5
4.1 LOCATION AND AMBIENT CONDITIONS............................................................................................................ 5
4.2 SHELVES AND USABLE SPACE ..................................................................................................................... 6
4.3 WATER TRAY ........................................................................................................................................... 6
4.4 GAS CONNECTION .................................................................................................................................... 6
4.5 ELECTRICAL CONNECTION .......................................................................................................................... 6

5 GETTING STARTED ....................................................................................................................................7

5.1 START UP ..............................................................................................................................................7
5.2 SETTING TIME AND DATE ............................................................................................................................ 7
5.3 FACTORY PRESETS ................................................................................................................................... 7
5.4 CHANGING TEMPERATURE SET POINTS AND HIGH / LOW ALARMS ........................................................................ 7
5.5 CHANGING CO2 SET POINT AND ALARM THRESHOLDS ....................................................................................... 8
5.6 ALARM STATUS SCREEN ............................................................................................................................. 8

6 OTHER FEATURES ..................................................................................................................................... 9

6.1 GRAPHICAL DISPLAY ................................................................................................................................. 9
6.2 UTILITY MENU ..........................................................................................................................................9
6.3 DOOR ALARM MENU ................................................................................................................................10
6.4 INCUBATOR INFORMATION ......................................................................................................................... 10
6.5 HISTORICAL EVENT LOG ........................................................................................................................... 10
6.6 USER SECURITY .....................................................................................................................................10
6.6.1 ENTERING A USER NAME AND PASSWORD ............................................................................................... 10
6.6.2 ENABLING SECURITY ........................................................................................................................... 10
6.6.3 LOGGING OUT ....................................................................................................................................11
6.6.4 DISABLING SECURITY ...........................................................................................................................11

7 SD CARD DATA LOGGING ....................................................................................................................... 11

 7.1 START LOGGING ..................................................................................................................................... 11
7.2 CHANGING FILENAME .............................................................................................................................. 12
7.3 REMOVING THE SD CARD ......................................................................................................................... 12

8 HIGH TEMPERATURE STERILISATION .................................................................................................. 13

8.1 PREPARATION ........................................................................................................................................ 13
8.2 STARTING THE CYCLE.............................................................................................................................. 13
8.3 STERILISE CYCLE ................................................................................................................................... 13
8.4 COOLING PHASE ....................................................................................................................................14
8.5 ABORTING THE STERILISE CYCLE ............................................................................................................... 14

9 ERROR MESSAGE SCREENS ................................................................................................................. 14

10 OPTIONS ..................................................................................................................................................15
10.1 VOLT FREE ALARM RELAY (BMS) ................................................................................................................ 15
10.2 4-20 MA RETRANSMISSION (RETX) .............................................................................................................. 15
10.3 RS 232 SERIAL COMMUNICATION (232) ........................................................................................................ 15
10.4 ETHERNET COMMUNICATION (ETH) ............................................................................................................. 15
10.5 MULTI-DOOR (8ID)...................................................................................................................................15

11 ACCESSORIES .........................................................................................................................................15

12 CLEANING .................................................................................................................................................16
12.1 OUTER SURFACES ..................................................................................................................................16
12.2 INNER SURFACES....................................................................................................................................16
12.3 OXIDATION AT HIGH TEMPERATURE (COMFORT 190S ONLY) .............................................................................. 16

13 SERVICE ....................................................................................................................................................16
13.1 WARRANTY ...........................................................................................................................................16
13.2 PREVENTATIVE MAINTENANCE ................................................................................................................... 16
13.3 RETURNING A UNIT .................................................................................................................................16

14 DISPOSAL .................................................................................................................................................17

15 TECHNICAL INFORMATION......................................................................................................................17
15.1 TECHNICAL DATA .................................................................................................................................... 17
15.2 DIMENSIONAL DRAWINGS ......................................................................................................................... 17
 Instructions for use

1 SAFETY
These safety instructions are intended to be used alongside
any local safety guidelines, rules and regulations. If in
GRXEWSOHDVHFRQVXOWWKH+HDOWKDQG6DIHW\2I¿FHURI\RXU
1.1 LEGAL CONSIDERATIONS organisation.
Only skilled electricians or engineers authorised by MMM
It is important that you read this manual
can perform repairs or maintenance on this incubator. Only
completely and observe all instructions.
original spare parts from MMM can be used. There are no
Failure to follow these instructions may result
user serviceable parts within the incubator.
in personal injury, equipment damage and
incorrect performance of the incubator. MMM takes no responsibility for 3rd party accessories or
equipment used with this incubator.
This manual contains information for installing, operation
and maintenance of the incubator. It cannot cover all
possible uses and applications. Please contact your dealer Electrical hazard
or MMM directly if you have a special application or the Danger of death
manual does not describe your problem in enough detail.
Do not remove any panels from incubator.
The contents of this manual and the incubator are subject to Do not allow the incubator to become wet.
DANGER
change without notice.
Danger of burning
Risk of injury
1.2 INTENDED USE The glass door and inner chamber reach
The CO2 Cell Comfort 190 incubators are intended for 200 °C during sterilisation.
mammalian cell cultivation under typical conditions of Do not force the door open during
37.0 °C. The pH of the cell media can be controlled by HOT
sterilisation.
altering the CO2 concentration within the incubator. SURFACES
Other applications are not approved by MMM. Please Stability hazard
contact your dealer or MMM directly, for advice if you intend Risk of injury
to use this incubator for other applications. Damage to unit
Do not place the unit too close to the edge
of a bench.
1.3 SYMBOLS USED IN THIS MANUAL STABILITY
Do not lean on the door when open.
HAZARD
The following symbols are used throughout this manual:
Lifting hazard
Risk of injury
Damage to unit
Lift with 4 people
95 kg
Risk of injury Electrical Hot surface Lifting LIFTING Observe safe handling regulations
hazard hazard HAZARD

2 DESCRIPTION
Stability Compressed Asphyxiation Mandatory CO2&HOO&RPIRUWLQFXEDWRUVDUH¿WWHGZLWKWKHODWHVW
hazard gas hazard hazard regulation generation of programmable controller, ensuring precise
control of temperature and CO2 levels.
Accurate and stable heating throughout the entire chamber
LVHQVXUHGE\WKHXQLTXH000GHVLJQHG¿YHVLGHGGLUHFW
heating element. In addition a separate independently
Read Lift with Disconnect Important
FRQWUROOHGGRRUKHDWHULV¿WWHGWRHQVXUHWKDWWKHLQQHUJODVV
operating several from information
door is free from condensation.
manual persons electrical for correct
supply operation 7KHGHVLJQRIWKHVHHOHPHQWSUR¿OHVSURPRWHQDWXUDO
convection of the air within the chamber, ensuring even
temperature and gas distribution throughout the chamber.
1.4 GENERAL SAFETY INSTRUCTIONS CO2 Cell Comfort incubator has extra heaters that allow
high temperature 200 °C dry sterilisation of the incubator
7KLVLQFXEDWRULVLQWHQGHGIRUXVHE\VXLWDEO\TXDOL¿HG
laboratory personnel who are familiar with all precautions chamber.
and standard safety practices for working in a laboratory
environment.

CO2CELL Comfort 190_np_en 1301_mmm_V1.02 3

 Instructions for use

The sensor is not mounted within the chamber; instead a

2.1 CONSTRUCTION small pump passes the chamber atmosphere through the
The inner chamber is made from 316 grade stainless steel. sensor.
It is deep drawn from one piece with no welds and is The sensor does not have to be removed during high
electro-polished, meaning that the inner surfaces are temperature sterilisation.
smooth and easily cleaned.
The removable shelves, racking and water tray are also
made from stainless steel. 2.5 DIAGRAMS
The outer housing is made from stainless steel and powder
coated with anti-bacterial biocide paint.
2.5.1 OUTER VIEW

2.2 DOORS
The outer door has a heater on its inner face. This door is
an integral part of the temperature control system and must
be closed for the incubator to function normally.
The door is hinged on the right hand side. Left hand opening
is only available as an option at the point of ordering, retro-
¿WWLQJLVQRWSRVVLEOH
When this door is open, the CO2 valve is closed and
heater power is reduced to prevent over shoot of operating
conditions. The door is linked to the controller alarm system.
DSection 6.3
On the Comfort 190S incubator, 2 magnetic solenoid
locks prevent the outer door from being opened while the
incubator is in a high temperature sterilise cycle.
An inner glass door seals against a thermoplastic elastomer
gasket. This allows viewing of the samples within the 2.5.2 INNER VIEW
chamber without disturbing the temperature or atmosphere
of the chamber.
The incubator can be optionally equipped with a multi-door.
These multi-doors improve temperature and CO2 recovery
DIWHUDGRRURSHQLQJ0XOWLGRRUVFDQEHUHWUR¿WWHG
DSection 10.

2.3 CONTROL SYSTEM

The colour LCD touch screen display allows the user to
easily see operating conditions and to alter settings through
icon based menus.
Software controlled PID loops constantly monitor
temperature and CO2 values, making automatic adjustments
to keep the chamber atmosphere within limits.
The controller is equipped with a built in feature that logs
incubator data directly to a removable SD card. This
data is in Excel format, making it easy to view with a PC.
DSection 7. 2.5.3 REAR CONNECTIONS
$56VHULDOSRUWLV¿WWHGDVVWDQGDUG7KLVDOORZV\RX
to connect the incubator to a PC so that you can remotely
access the SD card data. PC software is required to do this.
Contact your dealer or MMM directly for advice.
Other optional communication options are Ethernet PC
interface and 4-20 mA retransmit. DSection 10.

2.4 CO2 SENSOR

CO2 is measured and controlled using a highly accurate
infra-red sensor. This sensor remains practically drift free
between calibration periods.

4 CO2CELL Comfort 190_np_en 1301_mmm_V1.02

 Instructions for use

3 DELIVERY, 3.3 STORAGE

If the incubator has been in a cold location for a
TRANSPORTATION period then it needs to acclimatise to room
temperature.
AND STORAGE 0RYHWKHLQFXEDWRUWRLWV¿QDOORFDWLRQDQGZDLWIRUKRXUV
before start up. This will allow any condensation that may
3.1 UNPACKING have formed to dry. It will also stop the incubator from
Inspect the packaging for signs of transportation damage. overshooting temperature set point.

Carefully undo the top of the cardboard packaging and

gently slide the packaging over the incubator. 4 INSTALLATION
Inspect the incubator and inform the carrier immediately if
there are any signs of transportation damage. 4.1 LOCATION AND AMBIENT
Remove any packaging material from within the chamber. CONDITIONS
Remove any protective coverings from the surfaces of the
The incubator can be placed on a table or worktop.
inner chamber.
3OHDVHHQVXUHWKDWWKHVLWHLVVXI¿FLHQWO\VWURQJ
Remove the operating manual and accessory kit from within enough to carry the weight of the incubator.
the chamber. Keep these safe. MMM do not recommend placing the incubator directly on
Please check that the accessory pack contains the following WKHÀRRUDVWKLVLQFUHDVHVWKHULVNRIFRQWDPLQDWLRQ
items. Contact your dealer with details of any shortages. Wheeled platform forms are available that lift the incubator
RIIWKHÀRRU$OWHUQDWLYHO\WZRLQFXEDWRUVFDQEHVWDFNHG
Comfort 190 accessory pack Qty using the stacking kit. DSection 10.
Stainless steel perforated shelves 4 Correct location of an incubator is very important. The
incubator is designed to work in a typical laboratory
Shelf racking (left and right hand) 1 set
environment.
Racking support bar 1
For good performance the ambient conditions should be at
Stainless steel water tray 1 least 6 °C below the working temperature.
Service port bungs 2 E.g. for a set point of 37.0 °C the ambient temperature must
Formatted 2Gb SD card 1 not exceed 31.0 °C.
If the difference between the set point and ambient
Instructions for use 1
temperatures is less than 6.0 °C then the set point can be
3.5 meter length of CO2 tubing with HEPA 1 H[FHHGHGDQGWHPSHUDWXUHÀXFWXDWLRQZLOORFFXU
¿OWHU
Our performance data was produced at the ideal ambient
Hose clips 2 condition of 25.0 °C. For best performance the ambient
Test report 1 WHPSHUDWXUHVKRXOGQRWGUDVWLFDOO\H[FHHGWKLV¿JXUH
IEC mains power lead 1 Maximum ambient humidity is 70 % rH, non-condensing.
&RQGHQVDWLRQFDQRFFXUZLWKLQWKHFKDPEHULIWKLV¿JXUHLV
exceeded.
3.2 LIFTING AND MOVING A poorly located incubator can experience temperature
These incubators are heavy. The weights are as follows: ÀXFWXDWLRQVSRRUFHOOJURZWKDQGFRQWDPLQDWLRQRIFHOO
Comfort 190 is 94,5 Kg cultures. These guidelines will ensure best performance:
Comfort 190S is 104 Kg x 7KHLQFXEDWRUVKRXOGEHVLWHGRQDÀDWHYHQVXUIDFH
Please refer to Manual Handling Policy of your organisation x It should be in a well ventilated and dry area, but not
before attempting to lift the incubators. sited directly in the path of ventilation ducts.
x It should be free from vibration.
Risk of unit sliding or tipping. x It should be kept away from heat sources e.g. radiators,
Risk of injury by lifting heavy loads. heating pipes, refrigeration plant.
Risk of damage to the unit. x It should not be in direct sunlight.
Always lift using the handles on the outside x To avoid contamination it should not be placed directly
of the incubator. RQWKHÀRRU
Lift with 4 people. x $OORZVXI¿FLHQWGLVWDQFHVEHWZHHQDGMDFHQWHTXLSPHQW
Do not lift by using the door.
x There should not be big swings in ambient temperature.
Leave a clearance of 50 mm from each side and 100 m from
the rear.
Level the incubator by adjusting the two front feet. Place a
small spirit level on the centre position of the middle shelf as
a guide.

CO2CELL Comfort 190_np_en 1301_mmm_V1.02 5

 Instructions for use

When it is level, secure the feet by tightening the lock nuts

with a 10 mm spanner.
4.4 GAS CONNECTION
Risk of injury. Compressed gas.
4.2 SHELVES AND USABLE SPACE Risk of damage to unit.

Each incubator is supplied with 4 perforated stainless steel

Observe relevant regulations for
shelves. The shelves locate into removable stainless steel
handling CO2
racking. The shelves incorporate an anti-tilt mechanism,
Risk of asphyxiation. Site must have
stopping the shelves from being pulled out too far.
adequate ventilation.
Each shelf measures 690 mm wide by 390 mm deep. The
minimum spacing between shelves is 80 mm. There are Risk of CO2 poisoning. Site must have
8 possible positions for the shelves. adequate ventilation.
The shelves and racking can be left in place during sterilisation.
The usable space within the incubator is the volume The incubator is designed for use with CO2 of
in which the conditions are within our published 99.5 % purity. Input pressure greater than 1.0 bar
data. (15 psi) will damage the incubator so a pressure
reducing valve (PRV) must be used.
The CO2 inlet to the incubator is via a 4 mm barbed push
¿WWLQJ
359¶VZLWKWKHFRUUHFW¿WWLQJVDQGRWKHUJDVDFFHVVRULHV
can be supplied by MMM. DSection 10.
For correct connection of CO2, follow these steps in order:
 &RQQHFWWKHVXSSOLHGWXELQJDQG+(3$¿OWHUWRWKHJDV
supply.
2) Fully close the PRV.
3) Open the main valve on the gas bottle or central supply.
4) Adjust the PRV pressure of the gas supply to between
0.3 bar and 0.7 bar (4.5 to 10 psi).
5) Close the main valve on the gas bottle / central supply.
A, B, C = Internal dimensions 6) Connect the other end of the tubing to the incubator CO2
a = distance from left and right wall = 70 mm inlet. Secure with the supplied clip.
b = distance from top = 70 mm
Repeat these steps whenever you change the CO2 bottle or
c = distance from front and back wall = 70 mm
supply.
d = distance from base = 70 mm
Turn off the main valve and disconnect from the CO2 supply
For practical purposes this is the perforated area on each
if the incubator is to be unused for extended periods.
shelf.
x Do not place any samples outside of this space.
x 'RQRWRYHU¿OOHDFKVKHOI 4.5 ELECTRICAL CONNECTION
x $OORZVXI¿FLHQWURRPEHWZHHQVDPSOHVWRDOORZIRU
DLUÀRZ Risk of injury.
x Do not use the top shelf for very deep samples. Risk of damage to unit. Observe correct
mains supply voltage.
4.3 WATER TRAY Risk of electrical shock. Do not connect
A stainless steel water tray is supplied which allows the with wet hands.
incubator to provide 90 %rH. No user serviceable parts. Do not
remove service panels.
3ODFHWKHWUD\LVRQWKHÀRRURIWKHLQFXEDWRUORFDWLQJLWZLWK
the side racking. Disconnect unit in an emergency.
&DUHIXOO\¿OOWKHWUD\ZLWKOLWUHVOLWUHVRIGLVWLOOHGVWHULOLVHG
water.
000UHFRPPHQGWKDW\RXFOHDQDQGUH¿OOWKHWUD\HYHU\ Before connecting the incubator, check that the electrical
week. supply corresponds with the values on the incubator data
SODWH,ILQGRXEWFRQVXOWDTXDOL¿HGHOHFWULFLDQ
Any spillages within the chamber should be removed and
FOHDQHGLPPHGLDWHO\:DWHUOHIWRQWKHFKDPEHUÀRRUZLOO The incubator is designed to operate from a single phase
cause condensation to form. electrical supply of: 230 V AC ±10 % 50/60 Hz.

Under no circumstances should any equipment be placed in This incubator MUST be connected to a protective earthed
WKHZDWHUWUD\RURQWKHFKDPEHUÀRRU supply.

Remove the water tray before moving the incubator. Further details can be found in the technical data.
DSection 15.1.
Empty the water tray if the incubator is switched off,
otherwise condensation will occur.

6 CO2CELL Comfort 190_np_en 1301_mmm_V1.02

 Instructions for use

Plug the supplied power lead into the IEC connector on the MAIN DISPLAY
rear of the incubator.
Plug the other end of the IEC lead into the electrical supply. The main screen shows chamber set points (SP) and
process values (PV). It also shows the status of any
In an emergency you may need to isolate alarm and has touch screen buttons for accessing further
the incubator from the electrical supply by functions.
disconnecting the mains supply plug. Make sure
that the mains supply plug is easily accessible.

5 GETTING STARTED Temperatureprocess value Chamber temperature set point

5.1 START UP
CO2 process value Chamber CO2 set point

Insert the pre-formatted SD card (supplied) into the slot to Sterilise cycle start Menu escape

the right of the controller.

Turn on the unit using the power switch on the rear panel.
The controller will boot up and perform a self-check.
5.4 CHANGING TEMPERATURE SET
POINTS AND HIGH / LOW ALARMS
5.2 SETTING TIME AND DATE
To change the temperature set point, touch this area of the
After the controller has booted up, the time and date screen
will be shown. Time and date are shown in the format
hh:mm:ss and dd/mm/yy.
main screen:
If the time and date is correct

press:
Touch here
Touch here to alter
to alter setset
pointpoint

Touch
Touch here to
here to alter
alter high alarm limit
high
Proceed to step 5.3
alarm limit
Touch here to alter low alarm limit

Touch here to alter low

If the time and date are incorrect press the time display. This alarm limit
screen will be shown:
Use the keypad to
Use the touch screen to enter change the set point.
the correct time. Maximum set point is
limited to 60.0 °C
Press WRFRQ¿UP Minimum set point is
selection. limited to 25 °C

Repeat these steps for setting

Press WRFRQ¿UP
the date. selection.

The high and low alarm limits are relative to the chamber set
5.3 FACTORY PRESETS point.
For instance if the set point is 37.0 °C and the high alarm
The following values are preset to allow you to work straight limit is + 1.0 °C then the alarm will trigger at 38.0 °C.
away:
If the set point is changed to 36.5 °C then the high alarm will
Parameter Value trigger at 37.5 °C.
Set Point, Temperature 37.0 °C
Set Point, CO2 5.0 % Use the keypad to
High temperature alarm +1.0 °C change the high alarm
relative to set point threshold.
Low temperature alarm -1.0 °C
relative to set point Press WRFRQ¿UP
High CO2 alarm +1.0 °C selection.
relative to set point
Low CO2 alarm -1.0 °C
relative to set point
Door open alarm timer 30 seconds

CO2CELL Comfort 190_np_en 1301_mmm_V1.02 7

 Instructions for use

Use the keypad to Use the keypad to

change the low alarm change the low alarm
threshold. threshold.
This must be negative This must be negative
VRUHPHPEHUWR¿UVW VRUHPHPEHUWR¿UVW

press press

Press WRFRQ¿UP Press WRFRQ¿UP

selection. selection.

5.5 CHANGING CO2 SET POINTS AND 5.6 ALARM STATUS SCREEN
ALARM THRESHOLDS
To change the temperature set point, touch this area of the The alarm icon gives a visual indication of alarm
status.
main screen: Pressing the alarm icon opens the alarm status screen:

Touch here to alter set

point

Touch here to alter

high alarm limit

Touch here to alter low

alarm limit

Use the keypad to

High temperature alarm Low CO2 alarm
change the set point.
Maximum set point is
limited to 20.0 % Low temperature alarm Door open alarm

Minimum set point is

limited to 0.0 % High CO2 alarm SD card alarm

Press to
Low gas pressure alarm
FRQ¿UPVHOHFWLRQ
In an alarm condition an audible buzzer sounds and the

If the CO2 set point is 0.0 % then CO2 control is alarm icon changes to red.
disabled.
Press this red alarm icon and the alarm status screen will
show the corresponding alarm will in red.
Use the keypad to For instance this shows high temperature alarm condition:
change the high alarm
threshold.
Pressing this will mute the alarm.
The alarm is now acknowledged and the icons show this by
Press WRFRQ¿UP
selection.
changing to yellow:
The icons remain yellow for as long as the incubator is in an
alarm condition.
This process is the same for all alarms.

8 CO2CELL Comfort 190_np_en 1301_mmm_V1.02

 Instructions for use

As an additional warning the colours of the process values The CO2 graph is viewed in the same manner.
change during alarm condition.
6.2 UTILITY MENU
The temperature process
value has changed On the main screen press the utility menu symbol:
colour from black to red.
The utility menu combines all of the incubator settings
The CO2 process value menus in one place. You will already be familiar with some
will change colour from of these.
blue to red when it
enters alarm conditions.

6 OTHER FEATURES
6.1 GRAPHICAL DISPLAY
The controller has a built in feature that allows the user
to view graphs of both temperature and CO2. It stores a
minimum of 3 days of graphical data. When the internal Clock menu. DSection 5.2
PHPRU\LVIXOOLWZLOORYHUZULWHWKHROGHVWGDWD¿UVW
Graphs are only stored while the unit is powered. Temperature menu. DSection 5.5
This feature is independent of the SD data logging function.
DSection 7. CO2 menu. DSection 5.6
Ö
On the main screen press the graph symbol:
User security menu. DSection 6.6
The graph menu
opens:
SD card menu. DSection 7

Choose which graph Door alarm menu. DSection 6.3

you wish to view

Alarm status menu. DSection 5.7

Historical event log. DSection 6.5

In this instance we are viewing the temperature graph:

Incubator information. DSection 6.4

User log out. DSection 6.6

Press the
y-axis limits.

The values can be

changed using the
keypad.

Press Use to view the temperature

stability against time.

To return to the Main Screen press the escape button

CO2CELL Comfort 190_np_en 1301_mmm_V1.02 9

 Instructions for use

6.3 DOOR ALARM MENU

Press the door icon:

The door has a

magnetic switch that
senses when the door
is opened.

6.6 USER SECURITY

The door icon turns red as soon as the door is opened. After
a set time the alarm will sound. This can be muted in the This incubator allows for a user name and password
alarm status menu. DSection 5.7 to be programmed.
This delay can be changed by pressing here. This stops any unauthorised changes to incubator settings.
The format is mm:ss.

6.6.1 ENTERING A USER NAME AND

6.4 INCUBATOR INFORMATION PASSWORD
To enter a user name and password press the User icon:
Press the info icon:
The information screen shows the incubator Serial Number
DVZHOODWZKDWUHOHDVHRIVRIWZDUH¿UPZDUHWKHFRQWUROOHU
is using. This information is important should you require
any assistance from MMM.

Press here on Line 1 to

enter user name.

Press: to
save.
Then press here to
enter password.

Press:
to save
The user name and password are now saved.

6.5 HISTORICAL EVENT LOG 6.6.2 ENABLING SECURITY

The controller has a built in feature that displays the time Press the security status icon:
and date of 100 historical events.
When 100 events have been logged the oldest event is The icon changes state to:
over-written.
Events include: alarms, alarm acknowledgment, door open/ Security feature is enabled and you are now logged
closed, power on/off, SD card insertion/removal,
XVHUORJRQORJRIIVWHULOLVHF\FOHVWDUW¿QLVKDERUW on as shown by the open padlock symbol:

Press the event log icon: Press to scroll

though the events.

10 CO2CELL Comfort 190_np_en 1301_mmm_V1.02

 Instructions for use

6.6.3 LOGGING OUT 7.1 START LOGGING

On the utility menu screen, press and hold the log out icon: If the card is already inserted when the incubator is powered
up then logging will start automatically.

You are logged out and the padlock icon is now locked:
If the SD symbol is grey then the incubator is
No changes to the incubator can be made without logging
back in.
For the Comfort 190S incubator only: logging. If it is red then it is not logging.
At this point the magnetic door locks are activated, stopping
any unauthorised access to the incubator.
Press the red SD icon in the Utility Menu. The Status
screen will state that the card is removed.
6.6.4 DISABLING SECURITY

Press the utility icon:

When prompted, enter the user name and password.

To disable security press:

Icon changes to:

Security is now disabled.

Insert the SD card into the slot to the right of the controller

7 SD CARD DATA (label side closest to controller).

The SD Card status screen shows that the controller is
LOGGING locating the data and preparing to log. This can take from a
IHZVHFRQGVWRDIHZPLQXWHVGHSHQGLQJRQGDWD¿OHVL]H
The incubator is supplied with a 2 GB SD card. This Please be patient.
SD card is pre-formatted to work with the
controller’s operating system.
Only cards supplied by MMM can be used. Using
any other type of card will not work with the
incubator and will cause the controller system to
crash.
The controller can log to a removable SD card located on
the front of the incubator.
Every 30 seconds the controller records the following data
to the SD card:
Date & Time
:KHQWKHFRQWUROOHUKDVORFDWHGWKHORJ¿OHWKHVWDWXVVFUHHQ
Chamber temperature set point (SP);
shows that it is logging data:
Chamber temperature process value (PV)
Door set point (SP);
Door process value (PV)
Door set point (SP);
Door process value (PV)
CO2 set point (SP);
CO2 process value (PV) Press to return
to the main screen
$Q\HYHQWVDUHDOVRORJJHGLQDVHSDUDWH¿OH
The data is stored in Excel format making it very easy to
read and use the data with a PC.
Data is only logged when the incubator is powered; however
data is not lost when power is removed.

CO2CELL Comfort 190_np_en 1301_mmm_V1.02 11

 Instructions for use

7.2 CHANGING FILENAME 7.3 REMOVING THE SD CARD

<RXFDQHDVLO\FKDQJHWKH¿OHQDPHWRVWDUWDQHZORJ Always perform the following steps before
3UHVVWKH¿OHQDPHRQWKHVFUHHQLQWKLVFDVH12345. Enter removing the SD Card. Failure to follow
DQHZ¿OHQDPHLQWKLVFDVH these steps will results in corrupted data.

On the SD Card status screen press the SD card tools icon:

Press to store selection.

Press the remove card

icon:

Press WRFRQ¿UPWKHQHZ¿OHQDPH

3UHVVWKHFRQ¿UP

icon:

7KHFRQWUROOHUZLOOWKHQORFDWHWKHQHZ¿OHDQGVWDUWORJJLQJ

It is now safe to
remove the SD card.

When the card is removed then the screen will automatically

change to this:

12 CO2CELL Comfort 190_np_en 1301_mmm_V1.02

 Instructions for use

This shows that the door locks have activated and that
8 HIGH TEMPERATURE Operator 6 is logged on.

STERILISATION
Risk of damage to unit.
The Comfort 190 is not intended to be used as
an autoclave and must not be used for sterilising
any other equipment. Press to
proceed.
Only the shelves, racking and water tray can be
left inside the incubator during sterilisation.

Risk of injury. Risk of burning. Internal surfaces

reach 200 °C

The Comfort 190S incubator is equipped with a high 8.3 STERILISE CYCLE
temperature sterilisation function. This raises the chamber
temperature to 200 °C for 2 hours. The sterilise cycle has now started.
Independent evaluation has shown this to be effective A temperature graph is plotted to show
against all spores and bacteria commonly associated with the duration of the cycle. The y-axis
laboratory incubator contamination. automatically changes its scale to 205 °C

8.1 PREPARATION This shows that

the heaters are
Make sure that you have entered a user name and operational
password as shown in DSection 6.6.1
Empty and dry the water tray. This can be placed back
inside the incubator.
Abort cycle icon
Clear any samples or equipment from the shelves. DSection 8.5
The shelves and racking can be left in place during the
sterilisation cycle.

The sterilise cycle consists of 3 distinct phases. They are:

8.2 STARTING THE CYCLE
x Heat up.
Press and hold the sterilise button for 5 seconds: During the heat up phase, maximum power is applied to
7KH¿UVWVWHULOLVHF\FOHFKHFNOLVWRSHQV the heating elements. CO2 control is disabled to protect the
sensor and the door locks are activated.
x 2 hour dwell.
Automatically carried The dwell phase starts once the chamber temperature
out by controller. reaches 200 °C.
Heater power is automatically reduced to regulate the
temperature at 200 °C.
x Cool down.
After 2 hours at 200 °C power is removed from the heaters
and the cooling phase begins. The chamber is allowed to
cool naturally. When the chamber temperature reaches
38 °C the door locks are deactivated and CO2 control is
When you have emptied the water tray, place it back inside enabled.
the chamber and close the doors. The time taken for a full sterilisation cycle is
Press the grey tick to acknowledge this: dependent on the cool down phase.
As cooling relies on the heat loss through the chamber walls
All checks are now the time taken is affected by the location of the incubator
complete. and the ambient temperature.
So, an incubator placed under a worktop will take longer to
cool than a freestanding incubator on a worktop.
Press to
proceed. We recommend that you carry out the sterilise cycle at the
end of a working day. That way, when you return to work in
the morning the incubator will be ready for you to use.

CO2CELL Comfort 190_np_en 1301_mmm_V1.02 13

 Instructions for use

7KLVVWHULOLVHF\FOHJUDSKVKRZVDW\SLFDOVWDUWWR¿QLVK
duration of 8 hours:
8.5 ABORTING THE STERILISE CYCLE
Should you need to stop the sterilise cycle for any reason

then press the abort icon:

8.4 COOLING PHASE

After the 2 hour dwell is completed the incubator goes into
the cooling phase of the sterilisation cycle.
The doors are locked during this phase. CO2 control is
disabled.

The chamber
temperature is
displayed

Press The incubator now enters the cooling phase.

DSection 8.4.

The cooling phase ends once the temperature falls below

38.0 °C
9 ERROR MESSAGE
SCREENS
In the event of a fault condition the control system may warn
you with various error messages.
Chamber probe failure

Press to continue Chamber probe is out of

to main screen. limits and has failed.
Contact MMM Service
immediately
Base probe failure
Base probe is out of limits
and has failed.
Contact MMM Service
immediately
Door probe failure
Door probe is out of limits
and has failed.
Contact MMM Service
immediately

14 CO2CELL Comfort 190_np_en 1301_mmm_V1.02

 Instructions for use

SD card critical error The incubator will be supplied with a pre-wired 3 metre
SD has not been safely cable socket.
removed.

Press and hold Switch

power off, insert card and
switch power on again.
If problem persists contact
MMM Service
SD card removal error
Repeat the steps above to The temperature signal is scaled as: 4 mA = 0 °C;
¿[WKLV 20 mA = 250 °C
The CO2 signal is scaled as: 0 mA = 0 % CO2;
20 mA = 20 % CO2
Boot screen error
Take a picture of the 10.3 RS 232 SERIAL COMMUNICATION
screen. Turn power off for
1 minute.
(232)
56FRPPXQLFDWLRQLV¿WWHGDVVWDQGDUG7KLVDOORZVIRU
If problem persists contact
the SD card data to be remotely accessed and downloaded.
MMM Service with details
of picture. A PC with a serial (COM) port is required. Software is
required. Please contact MMM limited for further details.

10 OPTIONS 10.4 ETHERNET COMMUNICATION (ETH)

7KLVLVDQRSWLRQWKDWPXVWEHVSHFL¿HGDWWKHSRLQWRI
10.1 VOLT FREE ALARM RELAY (BMS) ordering.
$Q(WKHUQHWSRUWFDQEH¿WWHGWRFRQQHFWWKHLQFXEDWRUWRD
$OOWRXFKVFUHHQLQFXEDWRUVDUH¿WWHGDVVWDQGDUGZLWKD network. This allows the SD card to be remotely accessed
volt free changeover relay for connection to a building and for the incubator screen to be displayed on a PC.
maintenance system (BMS).
Connection to a PC network is required. Contact your IT
This relay changes state whenever the incubator is in an manager to see if this possible.
alarm condition. The relay changes back to its original state
Instructions will be sent separately when this option is
once the alarm condition clears.
ordered.
The connections are as shown below:

10.5 MULTI-DOOR (8ID)

7KLVFDQEHUHWUR¿WWHG
The inner glass door can be replaced with a multi-door. This
has 8 smaller glass doors in a stainless steel frame.
By only opening one small door to access the sample you
require, less heat, humidity and CO2 are lost.
This improves recovery of the chamber back to ideal
conditions and reduces the amount of CO2 used.

11 ACCESSORIES
10.2 4 - 20 mA RETRANSMISSION (RETX) This is the list of some of the accessories currently
7KLVLVDQRSWLRQWKDWPXVWEHVSHFL¿HGDWWKHSRLQWRI available.
ordering. Please contact MMM if the part you require is not listed.
This feature allows the chamber temperature and CO2 PTSK Stacking kit for 2 x Comfort 190
process values to be retransmitted as a 4 - 20 mA signal.
This 4 - 20 mA signal can be connected to external PTST1 Wheeled platform for Comfort 190
equipment for independent validation of incubator PRV Single stage CO2 pressure reducing valve
performance. PRV2 Two stage CO2 pressure reducing valve

CO2CELL Comfort 190_np_en 1301_mmm_V1.02 15

 Instructions for use

RO6 In line CO2 reducing valve with gauge 12.3 OXIDATION AT HIGH TEMPERATURE
(0.1 to 2 bar) (COMFORT 190S ONLY)
PNEU Automatic change over unit for two CO2
When operated at 200 °C the action of atmospheric oxygen
cylinders
may cause discolouration of the stainless steel surfaces.
TYGON Tygon gas tubing, per meter This natural oxidation is not detrimental to the quality of the
incubator and is completely harmless.

12 CLEANING 13 SERVICE
Electrical hazard. Do not spill water or 13.1 WARRANTY
FOHDQLQJÀXLGVRYHUWKHLQQHURURXWHU
surfaces. All MMM Comfort 190 incubators are supplied with a two
year manufacturer’s warranty from the date of installation.
Disconnect before cleaning. 7RUHJLVWHU\RXUZDUUDQW\SOHDVH¿OOLQWKH8VHU*XDUDQWHH
Completely dry the unit before Registration Card and post to MMM Limited or to your local
reconnecting the power. distributor.

13.2 PREVENTATIVE MAINTENANCE

This Culture Safe CO2 Incubator is built to last for many
12.1 OUTER SURFACES years, but to ensure that the unit continues to perform
Always disconnect the incubator before cleaning. to the highest level we recommend that you purchase a
preventative maintenance contract from MMM.
It is recommended that the exterior of the incubator regularly
wiped clean. Use a non-abrasive soft cloth dampened with a The incubator should be calibrated annually.
warm soapy water solution.
Make sure the exterior is thoroughly dry after cleaning. 13.3 RETURNING A UNIT
Should your incubator require repair or maintenance then
12.2 INNER SURFACES FRQWDFW\RXUORFDOGLVWULEXWRULQWKH¿UVWLQVWDQFH
It is advisable to clean the chamber regularly. Returns to MMM will only be accepted with prior
Use a 70 % isopropanol 30 % distilled water solution. authorisation from the Service Department.
Apply using a sterile cloth so as not to introduce any We recommend that you keep all packaging.
contamination. When returning a unit please include the following
Never use any of the following: information:
x Any chlorine based detergents or bleaches. x Incubator model and serial number
x Any acidic cleaning agents. x Date of purchase
x Sodium Azide
x Authorisation number
x Iodine
x Ferric Chloride
x Name of local distributor if not purchased from MMM

Wipe over the inner surfaces of the stainless chamber, all x A detailed description of the fault (we can never have too
the shelves including the underside of the shelves, the shelf much information)
racks and the water tray. x Where was the incubator located
The shelves, racking and water tray can be sterilised in an
x Contact details of the person who reported the fault
autoclave if required.
Clean the glass door and the inner gasket seal. x $FRPSOHWHGGHFRQWDPLQDWLRQFHUWL¿FDWHSOXVDFRS\
faxed through in advance)
Clean the inner stainless steel surfaces of the outer door
and the outer door gasket. MMM cannot accept any returns without a completed
GHFRQWDPLQDWLRQFHUWL¿FDWH

16 CO2CELL Comfort 190_np_en 1301_mmm_V1.02

 Instructions for use

14 DISPOSAL Volume
Number of shelves
Litres (cu. ft.) 190 (6.7)
4 shelves. Maximum of 8
The incubator must not be disposed of at public waste SHIPPING DIMENSIONS
collection points. It must only be disposed of by licensed Width mm (inches) 925 (36.4)
waste recycling companies. Height mm (inches) 1080 (42.5)
Contact MMM for further information regarding WEEE Depth mm (inches) 850 (33.5)
regulations. WEIGHT
Comfort 190, Net kg (lbs) 94.5 (208)
Comfort 190S, Net kg (lbs) 104 (229)

15 TECHNICAL Comfort 190, Shipping

Comfort 190S, Shipping
kg (lbs)
kg (lbs)
109 (240)
117.5 (259)

INFORMATION
15.1 TECHNICAL DATA 15.2 DIMENSIONAL DRAWINGS

TEMPERATURE MANAGEMENT
Range °C 5 °C above ambient to
60 °C
Control °C 0.1 °C
Stability °C ±0.1 °C @ 37.0 °C
Uniformity °C ±0.25 °C @ 37.0 °C
Door recovery,30 s minutes 13
opening
CO2 MANAGEMENT
Range % CO2 0-20 %
Control % CO2 ±0.1 %
Stability % CO2 ±0.2 %
Uniformity % CO2 ±0.2 %
Door recovery, 30 s minutes 6
opening
CO2 sensor type NDIR
CO2 inlet pressure Bar 0.3 to 0.7
CO2 inlet connection type PPSXVK¿WEDUEHGW\SH
HUMIDITY MANAGEMENT
Humidity control type Passive
Range % rH 90 % ± 5 %
Door recovery, 30 s minutes 50
opening
ELECTRICAL DATA
Nominal voltage 50/60 Hz V 230 1N ~PE
Power; Comfort 190 W 380
Power ; Comfort 190S W 1800
sterilise cycle
Power consumption kWh 0.058
Main fuse F1; Comfort 190 6.3 A / 6.3 x 32mm / 250 V / TT
Main fuse F1; 10 A / 6.3 x 32mm / 250 V / TT
Comfort 190S
EXTERIOR DIMENSIONS
Width mm (inches) 765 (30.1)
Height mm (inches) 862 (34)
Depth mm (inches) 734 (28.9)
Wall clearance, rear mm (inches) 100 (3.94)
Wall clearance, side mm (inches) 50 (1.97)
INTERIOR DIMENSIONS
Width mm (inches) 632 (24.9)
Height mm (inches) 686 (27)
Depth mm (inches) 440 (17.3)

CO2CELL Comfort 190_np_en 1301_mmm_V1.02 17

 Instructions for use

18 CO2CELL Comfort 190_np_en 1301_mmm_V1.02

:

, 2 8 ; , 1 3 8 4 ) 0 - 2 3 < ) = >

 
 
       
 
   




 
       


          
 


   

  ! " # " # " " $ $ % $ &

'  ! " # " # " " $ $ % ( &

) * + , - . / + ) 0 1 ) 2 3 ) 4 5 + + + 6 4 7 8 9 1 7 +