ORPHEE

19, chemin du champ-des-filles

CH-1228 Geneva / Plan-les-Ouates
SWITZERLAND

Tel : +41 (0) 22 706 1840

Fax : +41 (0) 22 794 4391
http://www.orphee-medical.com

MYTHIC 60

SERVICE MANUAL
REF : M60-SM-000 Rev 02
 REF : M60-SM-000 Rev 02

REVISIONS

Revision Nb Date Author Software Comments

01 02/12/19 MG > V 0.7.3 Creation

02 28/02/20 MG V 0.7.4 Cosmetic fixes
Updates of section: 3.2.1,3.3,3.4,4.6,5,6,7,9

CONTACT ADDRESS

MANUFACTURER
Manufactured in Germany for:
ORPHEE SA
19, chemin du champ des filles
CH-1228 Geneva / Plan-les-Ouates
SWITZERLAND

Tel : +41 (0) 22 706 1840

Fax : +41 (0) 22 794 4391
http://www.orphee-medical.com

LOCAL REPRESENTATIVE

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IMPORTANT SAFETY INSTRUCTIONS

WARNING! RISK OF DANGER! Indicates a procedure to be strictly respected

in order to avoid any risks for the operator (user) or damages on the instrument
or on the quality of results.
Indicates that wearing gloves is mandatory before performing the described
operation due to risk of contact with materials that may be infectious.

Indicates hot temperatures surfaces and risk of burns Do not touch directly
while the machine is turned on. To avoid risk of burning injuries, be sure to
wait 30 min after the device power is off before directly touching the areas
marked with this symbol.
Indicates that moving parts can cut and crush. Risk of pinch point.

Keep hands clear while operating the machine.

NOTE Indicates important additional information.

BEFORE TO INSTALL OR TO
MAINTAIN A MYTHIC 60 YOU
HAVE TO READ AND TO REFER
TO THE USER’S MANUAL.

OPERATORS MUST HAVE

RECEIVED ADEQUATE TRAINING.

BE CAREFUL WITH THE EDGE OF THE METAL SHEETS

AFTER HAVING DISMANTLED THE COVERS.

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TABLE OF CONTENTS
1. INTRODUCTION 6
2. FLUIDICS 7
2.1 GENERALITIES .......................................................................................................................................................................................................... 7
2.2 MYTHIC 60 ANALYSE CYCLE - FLUIDIC SEQUENCES DESCRIPTION ..................................................................................................................... 7
2.2 FLUDICS DIAGRAMS DESCRIPTIONS ..................................................................................................................................................................... 11
2.3 FLUIDICS VIEW ...................................................................................................................................................................................................... 14
2.3.1 Fluidics View (right side of instrument) ................................................................................................................................................ 14
2.3.2 Fluidics View (left side of instrument) ................................................................................................................................................. 15
2.4 FLUIDICS PARTS DESCRIPTION ............................................................................................................................................................................. 16
2.4.1 Sampling module ......................................................................................................................................................................................... 16
2.4.2 Counting module ......................................................................................................................................................................................... 16
2.4.3 Syringe module ........................................................................................................................................................................................... 16
2.4.4 Syringe valves............................................................................................................................................................................................. 16
2.4.5 Optical module ............................................................................................................................................................................................ 17
2.4.6 2 valves module .......................................................................................................................................................................................... 17
2.4.7 Counting valves ........................................................................................................................................................................................... 17
2.5 MODULES AND PARTS DESCRIPTIONS ................................................................................................................................................................... 18
2.5.1 Sampling module ......................................................................................................................................................................................... 18
2.5.2 Counting module ......................................................................................................................................................................................... 19
2.5.3 Syringe Module........................................................................................................................................................................................... 21
2.5.4 Syringe Valves Module ..............................................................................................................................................................................22
2.5.5 2 valves modules #1, #2, and counting valves .....................................................................................................................................23
2.5.6 Heating system ..........................................................................................................................................................................................24
2.5.7 Vacuum tank ................................................................................................................................................................................................24
2.5.8 Rinsing pump circuit ..................................................................................................................................................................................24
2.5.9 Optical module ............................................................................................................................................................................................25
3. ELECTRIC & ELECTRONIC 26
3.1 GENERALITIES ........................................................................................................................................................................................................26
3.2 ELECTRICS PARTS VIEW .........................................................................................................................................................................................26
3.2.1 CPU Board ....................................................................................................................................................................................................26
3.2.2 LCD screen ..................................................................................................................................................................................................27
3.2.3 Connection board .......................................................................................................................................................................................27
3.2.4 Power supply block .....................................................................................................................................................................................27
3.3 CPU BOARD TEST POINTS ......................................................................................................................................................................................28
3.4 CPU BOARD CONNECTIONS DIAGRAM ................................................................................................................................................................... 31
4 FLAGS & ALARMS 32
4.1 GENERAL FLAGS ......................................................................................................................................................................................................32
4.2 LEUCOCYTE FLAGS..................................................................................................................................................................................................33
4.3 ERYTHROCYTE AND HGB FLAGS ............................................................................................................................................................................35
4.4 PLATELET FLAGS.....................................................................................................................................................................................................36
4.5 SYSTEM ALERTS .....................................................................................................................................................................................................37
4.6 PRESSURE CONTROL SYSTEM .................................................................................................................................................................................38
5. OUTPUT FORMAT 51
5.1 CONNECTION TYPES................................................................................................................................................................................................ 51
5.2 SEIS M60 FORMAT C2 ........................................................................................................................................................................................ 51
6. PARTS LIST 68
6.1 SPARE PARTS LIST ..................................................................................................................................................................................................68
6.2 TWO/THREE YEARS SPARE PARTS KIT...................................................................................................................................................................70
7. SOFTWARE 71
7.1 INTRODUCTION ...................................................................................................................................................................................................... 71
7.2 SPECIFIC TECHNICIAN DISPLAY ............................................................................................................................................................................ 71
7.2.1 Technician password .................................................................................................................................................................................. 71
7.2.2 Technician display ......................................................................................................................................................................................72
7.2.2.1 Technician..........................................................................................................................................................................................72
7.2.2.2 Adjust dif .........................................................................................................................................................................................72
7.2.2.3 Adjust other.....................................................................................................................................................................................73
7.2.2.4 Check dif WB ...................................................................................................................................................................................74
7.2.2.5 QC dif tresholds .............................................................................................................................................................................74
7.2.2.6 Maintenance......................................................................................................................................................................................75
7.2.2.7 System config ..................................................................................................................................................................................75
7.2.3 Troubleshooting .........................................................................................................................................................................................76
7.2.3.1 Menu overview ..................................................................................................................................................................................76
7.2.3.2 Check sensors / valves ...................................................................................................................................................................77

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7.2 SOFTWARE TREE ....................................................................................................................................................................................................78
8. MAINTENANCE 80
8.1 MAINTENANCE TABLE ............................................................................................................................................................................................80
8.2 FLUSH CLEANING ................................................................................................................................................................................................... 81
8.3 SYRINGES GREASING.............................................................................................................................................................................................82
9. REPAIRING 83
9.1 EMERGENCY STOP ...................................................................................................................................................................................................83
9.2 SYRINGE VALVES MODULE #1 ..............................................................................................................................................................................83
9.3 SYRINGE VALVES MODULE #2 ..............................................................................................................................................................................88
9.4 COUNTING MODULE (RBC/WBC CHAMBERS, HEADS, ELECTRODES, COUNTING HOLDER) ............................................................................... 93
9.5 COUNTING VALVES MODULE DISASSEMBLY....................................................................................................................................................... 106
9.6 HEATER MODULE DISASSEMBLY ......................................................................................................................................................................... 109
9.7 IN LINE PRESSURE SENSOR DISASSEMBLY ........................................................................................................................................................113
9.8 OPTIC MODULE DISASSEMBLY .............................................................................................................................................................................116
9.9 CPU BOARD ............................................................................................................................................................................................................131
9.10 USB BOARD REPLACEMENT................................................................................................................................................................................. 135
9.11 REAR PANEL BOARD REPLACEMENT ..................................................................................................................................................................... 137
9.12 HGB BOARD REPLACEMENT ................................................................................................................................................................................ 139
9.13 PUMP DISASSEMBLY ............................................................................................................................................................................................141
9.14 SAMPLING MODULE DISASSEMBLY ................................................................................................................................................................... 146
9.15 ROCKER REPLACEMENT ........................................................................................................................................................................................ 156
9.16 PROBE BELT & PROBE CARRIAGE REPLACEMENT.................................................................................................................................................. 172
9.17 ROCKER STEPPER MOTOR REPLACEMENT............................................................................................................................................................. 184
9.18 STOPCOCK DISASSEMBLY................................................................................................................................................................................... 193
9.19 SYRINGE MODULE .............................................................................................................................................................................................. 197
9.20 SYRINGE VALVES MODULE ................................................................................................................................................................................. 212
9.21 AMBIENT TEMPERATURE SENSOR DISASSEMBLY ............................................................................................................................................ 215
9.22 DILUENT TEMPERATURE SENSOR REPLACEMENT. ............................................................................................................................................. 218
9.23 DISCHARGE TUBING DISASSEMBLY ................................................................................................................................................................. 221
10. TROUBLESHOOTING 225
10.1 ANALYTICAL PROBLEMS ..................................................................................................................................................................................... 225
10.2 MECHANICAL PROBLEMS TROUBLESHOOTING ................................................................................................................................................. 226
10.3 TROUBLESHOOTING MESSAGE.......................................................................................................................................................................... 227

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1. INTRODUCTION

BEFORE TO INSTALL OR TO
MAINTAIN A MYTHIC 60 YOU HAVE
TO READ AND TO REFER TO THE
USER’S MANUAL

The following chapters describe the different technical parts of the MYTHIC 60
- Technical specification
- Fluidic
- Electric & electronic
- Flags and alarms
- Output format
- Spare parts list
- Software description
- Maintenance procedure
- Repairing
- Troubleshooting procedures

General Specifications
Working Temp: Screen: Sampling modes:
18 to 32°C Touch-screen LCD with LED Whole Blood (WB):
Relative humidity: backlit, 8.4 inch (800x600) sampling volume = 15.6 µl
80% max. at 32°C Pre-diluted (PD):
Storage Temp: Connections: sampling volume = 20µL
-10°C to 50°C 5 USB ports
Altitude: Ethernet-RJ45 Throughput:
Up to 3000 meters Serial LIS-SUBD 9 pin (male) 60 samples/hour
Power supply
Dimensions (mm): Storage:
406x275x430 External supported Accessories: Up to 35 000 patients
Weight (kg): 13 Included:
1D USB Barcode reader
Power supply: USB keyboard (QWERTY)
Input: Optional:
100 to 240 VAC 50 to 60 Hz External printers
Output:
24V – 6.67 A – 160W

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2. FLUIDICS
2.1 Generalities
MYTHIC 60 is a fully automated analyzer performing hematological analysis on whole blood.
The human blood venous sample must be collected in an EDTA K3 (Ethylene Diamine Tetracetic Acid, tri
potassic) tube in sufficient quantity. The DIFF results are available for 24 hours after the blood draw. The
use of EDTA K2 leads to a poor quality level of the DIFF results.
It must be correctly homogenized before analysis. It is recommended to use a rotary agitator turning between
20 to 30 turns/mn during 10 minutes.

A volume of insufficient blood for the quantity of anticoagulant or a bad mixing may involve
an erroneous result.

2.2 Mythic 60 Analyse Cycle - Fluidic Sequences Description

SAMPLE ASPIRATION AND SAMPLING PROBE CLEANING:

ACTION COMMENT
HGB led on HGB Green led located in WBC counting chamber is
1
switched on
2 Read HGB Blank HGB led measure through diluent for HGB reference

3 Syringe DOWN Vacuum tank draining

Syringe DOWN Sample aspiration in the sampling probe

4

Sampling probe UP + Syringe UP +Pump Sampling probe outside rinsing with syringe and pump
5
ON
Sampling module moves to home position
6 Sampling module and sampling probe in home position

Sequence duration: 5 secs, cycle duration: 5 secs

SAMPLING PROBE MOVES TO WBC COUNTING CHAMBER AND WASTE CHAMBERS DRAINING:
ACTION COMMENT

1 Syringe UP Waste chambers draining

Sampling module moves to WBC Sampling probe located above the WBC counting chamber
2
counting chamber
Sampling probe DOWN Sampling probe moves to rinsing position in WBC counting
3
chamber
Syringe UP Sampling probe outside rinsing with syringe in WBC
4
counting chamber
Sequence duration: 2 secs, cycle duration: 7 secs

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WBC COUNTING CHAMBER DRAINING AND SAMPLING PROBE POSITIONING:
ACTION COMMENT

1 Syringe DOWN + Pump ON WBC counting chamber draining + Transfer tubing draining

Sampling probe DOWN Sampling probe moves to dilution position in WBC counting
2
bath
3 Syringe UP Waste chambers draining

Sequence duration: 3.25 secs, cycle duration: 10.25 secs

FIRST DILUTION AND BUBBLING IN WBC COUNTING CHAMBER:

ACTION COMMENT
Syringe UP Diluent (by entry on WBC counting chamber) in WBC
1
counting chamber for first dilution
Sample + Diluent (through the sampling probe) dispense in
2 Syringe UP WBC counting chamber for dilution

3 Syringe UP First sample and diluent bubbling in WBC counting chamber

Sequence duration: 5.25 secs, cycle duration: 15.5 secs

FIRST DILUTION ASPIRATION, SAMPLING PROBE CLEANING:

ACTION COMMENT
Syringe DOWN RBC counting chamber partial draining and diluent removal
1 on WBC counting chamber entry
Syringe DOWN and Sampling probe UP First dilution aspiration in sampling probe
2

Sampling probe UP + Syringe UP + Pump Sampling probe moves to home position and sampling probe
3
ON outside rinsing
Sampling module moves to RBC counting Sampling module in RBC dilution position
4
chamber
Sampling probe DOWN Sampling probe moves to rinsing position in RBC counting
5
chamber
Sequence duration: 5.25 secs, cycle duration: 20.75 secs

LYSE IN WBC COUNTING CHAMBER AND BUBBLING:

ACTION COMMENT
Syringe UP Lyse dispense in WBC counting chamber + Sampling probe
1 outside rinsing in RBC counting chamber

2 Syringe UP End of lyse dispense in WBC counting chamber

3 Syringe UP Second bubbling in WBC counting chamber

Sequence duration: 2.5 secs, cycle duration: 23.25 secs

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RBC COUNTING CHAMBER DRAINING:
ACTION COMMENT
RBC counting chamber draining and lyse removal on WBC
1 Syringe DOWN + Pump ON counting chamber

2 Sampling probe DOWN Sampling probe moves to dilution position in RBC counting
chamber
3 Syringe UP Waste chambers draining

Sequence duration: 2.25 secs, cycle duration: 25.5 secs

SECOND DILUTION AND BUBBLING IN RBC COUNTING CHAMBER:

ACTION COMMENT

Diluent (by entry on RBC counting chamber) in RBC

1 Syringe UP counting chamber for RBC dilution

First dilution sample + Diluent (through the sampling

2 Syringe UP probe) dispense in RBC counting chamber for dilution

3 Measurement current ON

4 Optical LED ON

Syringe UP + Sampling probe UP Third bubbling in WBC counting chamber + Sampling probe
5 in home position

6 Syringe UP Bubbling in RBC counting chamber + counting heads rinsing

Sequence duration: 7 secs, cycle duration: 32.5 secs

FIRST COUNTING:
ACTION COMMENT

Waste chambers at atmosphere pressure Pressure control to “0” in waste chambers before vacuum
1
creation for counting
Syringe DOWN WBC dilution transferred to the optical bench and diluent
2
removal on RBC counting chamber
Syringe DOWN First and second dilutions (present in counting chambers)
3
aspiration through WBC and RBC counting heads
4 Wait 500ms Resistive counting vacuum stabilization

5 Check pressure Pressure control before resistive counting

Start first RES counting Cells counting acquisition during aspiration through WBC
6
and RBC counting heads
7 Stop first RES counting

Sequence duration: 8 secs, cycle duration: 40.5 secs

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BACK FLUSH IN COUNTING HEADS AND SECOND COUNTING:

ACTION COMMENT

Syringe UP Back flushes with diluent in counting heads to make sure

1 potential clogs did not happen during first counting
sequence
First and second dilutions (present in baths) aspiration
2 Syringe DOWN through WBC and RBC counting heads

Syringe UP and start optical WBC Injection of transferred sample in optical bench, WBC
3
differentiation differentiation
Start second RES counting Cells counting acquisition during aspiration through WBC
4
and RBC counting heads
5 Stop second RES counting

Sequence duration: 10.25 secs, cycle duration: 50.75 secs

COUNTING HEADS CLEANING:

ACTION COMMENT

1 Counting Heads cleaning Diluent behind counting heads is replaced by cleaner

2 Stop Optical WBC differentiation

3 Measurement current OFF

4 Optical LED OFF

Sequence duration: 1.75 secs, cycle duration: 52.5 secs

WBC COUNTING CHAMBER DRAINING AND FILLING:

ACTION COMMENT

Syringe DOWN + Pump ON + Sampling WBC counting chamber and transfer tubing draining,
1 probe DOWN sampling probe moves to rinsing position in RBC counting
chamber
2 Syringe UP WBC counting chamber filling with diluent

Syringe UP Back flushes with cleaner for cleaning counting heads

3
after counting sequences
Sequence duration: 2.5 secs, cycle duration: 55 secs

RBC COUNTING CHAMBER DRAINING AND FILLING:

ACTION COMMENT

1 Syringe DOWN + Pump ON RBC counting chamber draining and optical transfer priming

Syringe UP Diluent (through Sampling probe outside) dispense in RBC

2 counting
chamber + waste chambers draining
3 Sampling probe UP Sampling probe moves to home position

4 Pump ON Sampling probe drying through rinsing head

5 Syringe UP Diluent dispense in rinsing head to rinse the Sampling probe

Sequence duration: 2.5 secs, cycle duration: 57.5 secs

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SAMPLING MODULE MOVES TO WBC COUNTING CHAMBER AND SAMPLING PROBE INITIALIZATION
FOR NEXT SAMPLE:
ACTION COMMENT

Sampling module moves to home Sampling module in sampling position

1
position
2 Syringe UP Syringe moves to home position

3 Sampling probe DOWN Sampling probe in sampling position, ready for next sampling

Waste chambers to atmosphere

4
pressure
5 End of analytic cycle

Sequence duration: 2.5 secs, cycle duration: 60 secs

2.2 Fludics Diagrams Descriptions

All valves used in the analyzer are size 1,6mm.

Hydraulic connections are 1.2mm or 1.6mm depending on the color of the connector.
NOTE:

FLUDIC DIAGRAM:

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DILUENT LINE:

LYSE LINE:

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CLEANER LINE:

WASTE LINE:

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2.3 Fluidics view

All the fluidics part is on the right side of the instrument it’s consists a few modules :
- Sampling module :
o Rocker (patent pending) : Manages the rise and descent of the needle.
- Syringe module :
o Reagent syringes (Diluent, lysis), sampling and air syringes.
- Syringe Valves
- 2 valves module
- Counting valves
- Counting module:
o WBC and RBC counting chambers and hemoglobin measurement.
o Liquid valve manifold assembly and tubing.
- Optical module
All fluidic connections are realize by manifolds and tubes. Those tubes can be access from top and left side
of instrument (the metal bulkhead must be removed firs).

2.3.1 Fluidics View (right side of instrument)

Optical module

Sampling module

Syringe valves

2 valves module x2

Syringe module

Counting module

Counting valves

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2.3.2 Fluidics View (left side of instrument)

Needle motor

Vacuum tank

Inline pressure
sensor

Rocker motor

CPU board pressure

sensor tube

Reagent heater

Rinsing pump

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2.4 Fluidics parts description
The hydraulic part of the MYTHIC 60 is very simple and made of only 8 modules :
- Sampling module
- Counting module
- Syringe module
- Syringe valves
- Optical module
- 2 valves module x2
- Counting valves

The modules are connected together by semi rigid tubing.

2.4.1 Sampling module

This module (patent pending) enables to draw the sample and to perform the WBC and RBC/PLT dilutions.
It is assembled with a rotating rocker moving around a support which maintains the system to move up and
down the sampling needle.
A very reliable system of rack-gear moves the rocker.
The cleaning system of the sampling needle can be removed without tool (see section 9.7.6 in the user manual).
The o-ring of the needle included in the cleaning system can also be removed without tool.
The maintenance of these parts is very easy to perform.

2.4.2 Counting module

This module allows to count the WBC and RBC/PLT and to measure the HGB.
It is made with a manifold maintaining the reagent commutation valves and the counting bath block with their
measurement block including the apertures.

2.4.3 Syringe module

This module (patent pending) enables :
to draw the sample
to distribute the reagents
to drain the baths
to do the vacuum necessary for counting
and to push the waste to the waste container
Its contains the syringes bloc including five syringes :
sampling syringe
lysis syringe
two waste and vacuum/pressure syringes
diluent syringe.
Only one motor drives the five syringes.
The diluent input and the waste output are also included in this manifold.

2.4.4 Syringe valves

It is made with a manifold maintaining the fluid commutation valves for syringes.

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2.4.5 Optical module
This very important part allows to measure the leucocytes sub population.
It is made of three parts :
- an illumination module : which contain the semi-conductor light source, a beam adjustment glass smear, and
lens.
- a flow cell : made from glass in which samples flow.
- and the detection module : which contain lens, a beam separation glass smear, photodiodes and the amplifier
boars for the two measurement axes.

2.4.6 2 valves module

It a support plate for 2 valves, its contains connection for fluidic components.

2.4.7 Counting valves

It a support plate for 2 valves, its contains connection for fluidic components.

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2.5 Modules and parts descriptions
2.5.1 Sampling module

Needle Motor
Needle Guide

Needle Sensor
Board

Rocker Sensor
Rinsing Head

Rocker Motor

Rocker Needle

Belt Needle guide

O-ring

Washer

Belt tensioner Rinsing Head

Rocker guide

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2.5.2 Counting module

Protection cover

Counting manifold

RBC/PLT, WBC
counting baths

WBC counting
HB LED head

RBC/PLT
counting head

RBC/PLT
Counting Bath

RBC/PLT
electrode
WBC HB detection
electrode board

WBC/HGB
Counting Bath

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Counting manifold

Blocked fitting

Int. Ø1.6mm fitting

Int. Ø1.2mm fitting

Stainless steel fitting

Int. Ø1.2mm fitting

Electrodes connections

Int. Ø1.2mm fitting

Counting valves

Hydraulic connections

Counting valves
Counting valve manifold

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2.5.3 Syringe Module

Front view Rear View

Hydraulic
fittings
Syringe body

Optical
Sampling
sensor
piston
Plastic nut

Diluent
piston
Lead screw

Waste
pistons
Motor
support
Lyse piston Syringe
stepper motor

Designation
1 Syringe body assy 2 13

2 Syringe motor assy

Sampling piston
3
(DIA1.6mm) 10
Diluent piston (DIA
4 9
16mm)
1
Waste piston (DIA
5
22mm)
Lyse piston (DIA
6
6.5mm)
7
7 Screw CZX M4x12
8 Screw CZX M4x30 11

9 Screw K30x8
Syringe barbed 8
10 12
connector DIA 1.2
Syringe barbed 4 6
11
connector DIA 1.6
Syringe barbed
12 3
connector closed
Slotted optical
13 5
switch

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2.5.4 Syringe Valves Module

Designation
1 Valve 3/2
2 Valve 2/2
3 Tubing connector (Int-diam 1,2)
4 Tubing connector (Int-diam 1,6)
5 Syringe manifold

3
2
1

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2.5.5 2 valves modules #1, #2, and counting valves

2 valves modules #1 2 valves modules #2 counting valves

Designation
1 Valve 3/2
2 Valve 2/2
3 Tubing connector (Int-diam1,2)
4 Tubing connector (Int-diam1,6)
5 Valve manifold

5
4 5 4 3 2
3
1

2 valves modules #1 and #2 counting valves

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2.5.6 Heating system

Diluent Line (Fitting Ø1.6mm)

Lyse Line (Fitting Ø1.2mm)

2.5.7 Vacuum tank

38 39

37

2.5.8 Rinsing pump circuit

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2.5.9 Optical module

Detection part Flow cell Illumination part

flowcell

cuvette

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3. ELECTRIC & ELECTRONIC

3.1 Generalities
Only three boards compose the MYTHIC 60 :
- CPU main board (connects with display)
- Microprocessor Q7 board
- Connection board

- In the case of replacement of any electronic board, be aware of the sensibility of this
board to the electrical static discharge (ESD).
- Before operating check the earth connection and use the antistatic bag supply with the
board. Please contact Orphée for more information.
- Be careful with the edge of the metal sheets after having dismantled the covers.

3.2 Electrics parts view

3.2.1 CPU Board

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The CPU board is located on a front of the analyzer under the display.
The board, driven by a 32-bit processor, manages the following parts :
- Sample needle, rocker, syringe block motors movement
- Display and touch panel management
- Connection mode (RS232, Ethernet, USB)
- Printer, external keyboard, USB drive
- Measurement (Counting, hemoglobin measurement)
- Data processing
- External barcode reader

3.2.2 LCD screen

Its located on a front of a instrument. It will display GIU and sending input from touch panel.

P/S + Back light

Data

CPU BOARD

Touch screen

3.2.3 Connection board

This board is located on a back of analyzer. Its use to connect all ports to main board.

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3.2.4 Power supply block

MYTHIC 60 is supplied with an external power supply block.

- In the case of replacement of the main power wire supplied with the MYTHIC 60 the new
one must comply with the local regulation.
- The MYTHIC 60 has been certified with the power supply adapter provided with the
machine. The use of another external power supply is not guaranteed. Please contact your
Orphée representative for more information.

3.3 CPU board test points

+24V DC External Power Supply Input

TP1

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+24V DC Output of Protection IC.
TP2

+24V DC Output of Main Power Switch

TP3

+12V DC .Output DC/DC converter for

LCD Backlight Power Supply
TP4

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 REF : M60-SM-000 Rev 02
+3.3V DC .Output of DC/DC converter
Power Supply
TP5

+5V DC .Output of DC/DC converter

Power Supply
TP6

+3V DC .Lithium Cell

TP7

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 REF : M60-SM-000 Rev 02
3.4 CPU Board connections diagram

21

20
10
12

15

19
27
13
26 11 22 9
17 4 5
25

14 3 1
18
2 6

Cable Designation
1 Power Supply Cable
2 4 Valves Cable
3 14 Valves Cable
4 Fluidic Door Switch + Heater +Diluent Temp. sensor
5 CPU-Rear Panel Cable
6 Motors/sensors Cable
9 ON-OFF Cable
10 ALL coaxial cable
11 USB Cable
12 FSC coaxial cable
13 LCD Screen Cable
14 LCD Power supply + Back light
15 Touch screen Flat Cable
17 Counting Cable
18 Optic LED p/s Cable
19 Not Used
20 WBC Coaxial Cable
21 RBC Coaxial Cable
22 Ambient Temperature sensor
25 Start key switch Cable
26 Not Used
27 Not Used

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 REF : M60-SM-000 Rev 02

4 FLAGS & ALARMS

MYTHIC 60 manages different alarms. These alarms allow user to be alerted if there is a mistake
which can affect the quality of the results. These alarms appear on screen at the right of the result.

In presence of one or more alarms, it is recommended to check the result by a

conventional measure or on blood smear.

NOTE: Most of these alarms can be adjusted by the user.

4.1 General Flags

Each parameter value is accompanied with specific information per the automation characteristics.

XXXX+: Over range on linearity high limit.

XXXX!: Suspicious result. Occurs when a morphological alarm has been triggered (please see
chapters below) or when an associated parameter might be suspected (For instance, if WBC if
flagged with +, LYM, MON, NEU, EOS and BAS will be flagged with +).
++++ : Higher than the reportable limits. WBC, RBC, PLT, HCT, HGB.
----: Invalid result.
XXXX*: Rejected result.
h: Results higher than normal value.
L: Results lower than normal value.
H: Results higher than panic value.
L: Results lower than panic value.

NOTE: Whenever one of the following symbol is triggered: *,+, ++++, display tagged result in
bold (flag included).
Whenever one of the following flags is triggered: L, l, h, H, display tagged result normally and
flagged in bold.

Whenever “!” is triggered display afferent result in italic (flag included).

Change color of all flagged result to a specific color in accordance to the UI specifications (flag included):
• Orange (l, h) is used as standard color.
• Red (L, H) is used as standard color.

When result is flagged with: *, +, ++++,!, the parameter printout on table view is the parameter
value followed or replaced (++++) by the appropriate symbol.

When result is flagged with L,l, h or H, the parameter printout on table view is in reverse
video only.

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 REF : M60-SM-000 Rev 02
4.2 Leucocyte Flags
Extraction of distribution curve in height of pulses (after initial pulse width based cut-off).

L1 L5

Two editable
thresholds L1, L5 enable to discriminate the resistive count flags.

MORPHOLOGICAL ALARMS
On Curve
Alarm Short
Trigger Condition Alarm Flags enlarged
version
Review on Smear:
Determination of the population defined from channel CL1
Small lymphocytes,
to CL1-2.
Probable Incomplete,
The alarm is raised if this population is higher than an L1
Erythroblasts,
absolute limit AND a percentage of the lymphocyte
Platelet Aggregates,
population.
Erythroblasts
Determination of the population defined by CL5 threshold
and curve ending.
Review on Smear:
The alarm is raised if this population is higher than L5
Immature Cells.
an absolute limit AND a percentage of the WBC
population.

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 REF : M60-SM-000 Rev 02
The measured pulses on the two optical channels are displayed on DIF Plot ALL (Y axis) Vs
FSC (X axis).

Definition of height zones enable to discriminate optic count flags.

MORPHOLOGICAL ALARMS
On DIF Plot
Trigger Condition Alarm Short version Alarm Flags enlarged
Presence of cells in the zone regarding Review on Smear:
the total number of leukocytes in the N1 Debris or Small Cells,
DIF Plot. Platelet Aggregates.
Review on Smear:
Probable Incomplete,
Presence of cells in the zone regarding
N2 Erytrolysis,
the number of lymphocytes.
Platelet Aggregates,
Erythroblasts.
Review the DIF.
Presence of Immature Cells (from the
IMM Review on Smear:
mono or polynucleated cells line).
Immature Cells.
Review the DIF.
Presence of atypical lymphocytes. ALY Review on Smear:
Atypical Lymphocytes.
Review the DIF.
Presence of atypical lymphocytes or
RL Review on Smear:
basophiles.
Right Lymphocytes.
Presence of basophiles, small neutrophils
Review the DIF.
(without granulations or few HL
High Lymphocytes.
segmented), band cells.
Presence of small neutrophils (without
Review the DIF.
granulations or few segmented), band NL
Low Neutrophils.
cells or hyper basophil monocytes.
Presence of giant neutrophils, hyper
Review the DIF.
segmented neutrophils, eosinophils with NH
High Neutrophils.
few granulations or damaged eosinophils.

In any case if one or more DIF parameters (LYM, MON, NEU, EOS or BAS) are following
with a star (* ) the sample must be checked on smear.

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4.3 Erythrocyte and HGB Flags

Extraction of distribution curve in height of pulses (after initial pulse width based cut-off).
Two editable thresholds R1 and R2 enable to discriminate between microcytes, normocytes and macrocytes.

CR1 CR2

100% 68.26% of total

distribution area

RDW

RDW-

20%

MCV
 MCV is measured on the whole RBC acquisition.
 HCT parameter is calculated from MCV and RBC parameters.
 MCHC parameter is calculated from HGB and HCT parameters.
 MCH parameter is calculated from HGB and RBC parameters.
 RDW parameter is calculated on curve distribution (defined as the CV of 68.26% of
total distribution area).
 RDW-SD parameter is calculated on curve distribution (defined as curve width at 20%
of peak).

MORPHOLOGICAL ALARMS
On The Curve
Trigger Condition Alarm Short version Alarm Flags enlarged
Determination of the population defined from channel 0 to
CR1.
R1 Microcytes
The alarm is raised if this population is higher than an
absolute limit OR a percentage of the RBC population.
Determination of the population defined by CR2 threshold
and curve ending.
R2 Macrocytes
The alarm is raised if this population is higher than an
absolute limit OR a percentage of the RBC population.

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4.4 Platelet Flags
Extraction of distribution curve in height of pulses (after initial pulse width based cut-off), below an
editable threshold P that discriminates between PLT and RBC (sample dependent).

MORPHOLOGICAL ALARMS
On The Curve
Trigger Condition Alarm Short version Alarm Flags enlarged
Determination of the population defined from channel 0 to
CP1.The alarm is raised if this population is higher than an P1 Platelet Debris
absolute limit OR a percentage of the PLT population.
Determination of the population defined by CP2 threshold
and P.
P2 Schizocytes
The alarm is raised if this population is higher than an
absolute limit OR a percentage of the PLT population.
Determination of the population defined by CP3 threshold
and over a CP3-2 width at the right of CP3.
P3 Microcytes
The alarm is raised if this population is higher than an
absolute limit AND a percentage of the PLT population.

NOTE: Only P threshold is displayed on histogram.

 MPV parameter is calculated on curve distribution (defined as curve width at 20% of peak).
 PCT parameter is calculated from PLT and MPV parameters.
 P-LCR is calculated as the percentage of platelets with a volume higher than 12fL, i.e. those
appearing between 12fL and P.
 P-LCR is significantly decreased in patients with thrombocytosis while it is increased in
thrombocytopenia. In patients with high counts, P-LCR is significantly decreased in reactive
thrombocytosis than neoplastic thrombocytosis. P-LCR is more likely higher in destructive
thrombocytopenia than hypoproliferative thrombocytopenia. P-LCR is inversely related to
platelet count and directly related to PDW and MPV.

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4.5 System alerts
Pressure default
Means that pressure was out of range for at specific point in counting cycle. Check the tubing
connection of the pressure sensors, if all the tubing around the waste syringes are connected, if
there is no leak on the waste syringe piston

INS-T / Instrument Temperature Out Of Range

Means that the instrument temperature is lower than 17°C or higher than 33°C and the results
could be affected.

WBC BAL / WBC Balance

Unbalanced counting between the resistive and the optical measurement on the WBC channel. This may be
explained by a clog on the WBC aperture, a clog on the WBC flow cell or that the stop cock has been left as
closed.

WBC-CL / WBC Aperture Clog

Clog detected during WBC measurement.

RBC-CL / RBC Aperture Clog

Clog detected during RBC or PLT measurement.

O-DF / Optical Default

Unstable LED light low measurement during WBC DIF measurement. This may come from bubble or
unexpected phenomenon during WBC DIF measurement.

SU-F / Startup Failed

Background limits or HGB blank are out of range.

QC Not Done / QC Not Done During The Day

While no QC are done during the day. This system alert is set to every analysis until a QC sample is run.

QC Failed / Last Run QC Failed

The last QC result is out of the lot limits. This system alert is set to every analysis until a correct QC is
done.

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 REF : M60-SM-000 Rev 02
4.6 Pressure control system

Introduction
Mythic 60 is equipped with two pressure sensors:
 P1, located on CPU board, it controls the pressure generated in waste syringe.
 P2, located between valve #3 and rinse pump, it controls the pressure in tubing #10.

Fluidic schematic

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 REF : M60-SM-000 Rev 02

Summary of the pressure checks from analysis cycle

(software version ≥ V0.7.4-005 / fluidic cycle version V0.8.0.9)

Error Message Sensor Range Detail of Pressure Checking

ERROR: Check pressure in waste syringe after

1 P1 [-550, -200]
SYRINGE VACUUM DEFAULT drawing the blood sample

ERROR: Check pressure in tubing #10 during

2 P2 [-425, -50]
PUMP DEFAULT needle rinsing after sampling

ERROR: Check pressure in waste syringe

3 P1 [-600, -250]
NO DILUENT during WBC bath draining

ERROR: Check pressure in waste syringe

4 P1 [-150, +50]
DRAIN BATH DEFAULT after WBC bath draining

ERROR: Check pressure in waste syringe after

5 P1 [-50, +180]
WASTE DRAINING DEFAULT waste syringe draining

Check pressure in tubing #10 during

ERROR:
6 P2 [-425, -50] needle rinsing after drawing the first
PUMP DEFAULT
dilution

ERROR: Check pressure in waste syringe

7 P1 [-450, -200]
NO DILUENT during RBC bath draining

ERROR: Check pressure in waste syringe

8 P1 [-150, +50]
DRAIN BATH DEFAULT after RBC bath draining

ERROR: Check pressure in waste syringe after

9 P1 [-50, +180]
WASTE DRAINING DEFAULT waste syringe draining

Check pressure in waste syringe and

ERROR:
10 P1 [-300, -100] vacuum tank before first counting.
VACCUM COUNTING DEFAULT
Absolute Pressure Pcount is recorded.
Must be < Pcount Check pressure variation in vacuum
ERROR:
11 P1 increased by tank after the first counting sequence
VACUUM COUNTING DEFAULT
5% (3secs)
Check pressure in waste syringe and
ERROR:
12 P1 [-300, -100] vacuum tank before second counting
VACCUM COUNTING DEFAULT
sequence
ERROR: Check pressure in waste syringe after
13 DRAIN BATHDEFAULT (OR P1 [-150, +100] RBC bath draining and control the
CHECK FOR EV12 FAILURE) correct operation of the valve #12
PRESSURE DEFAULT: PUMP Check pressure in tubing #10 during
14 DEFAULT (OR CHECK FOR EV18 P2 [-425, -50] needle drying and the correct
FAILURE) operation of the valve #18

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 REF : M60-SM-000 Rev 02

Fluidic description of MYTHIC 60 analysis cycle

A. START ANALYSIS CYCLE (user press on start trigger)
STEP ACTION COMMENT
Front LED indicator warming user to keep sample tube in
1 Blink on
sampling position

B. DRAIN VACUUM TANK + BLANK HGB MEASUREMENT + SYRINGE CARRIAGE IN SAMPLING

POSITION
STEP ACTION COMMENT

1 Syringe moves down

Beginning of vacuum tank draining
2 Set EV8 + EV17

3 Wait 500 ms Ensure vacuum tank draining

4 Read HGB Blank HGB reference measurements

5 Reset EV8 +EV17 End of Vacuum tank draining

Note: Piston carriage against bottom E-clip of sampling
6 Wait motor syringe piston

C. BLOOD SAMPLE DRAW (15.6µl)

STEP ACTION COMMENT

1 Syringe moves down Sample draw 15.6µl + vacuum in waste syringe

2 Check pressure waste [-550; -200] Control the vacuum created in waste syringe

3 Blink off + buzzer Warm the user that sample tube can be removed

D. NEEDLE RINSING (0.766ML OF DILUENT)

STEP ACTION COMMENT

1 Pump on Start the pump to drain Tubing#10 from any liquid

2 Set EV4 Switch diluent line to rinse head

Push diluent to rinse head for needle rinsing (pump still on for
3 Syringe moves up
diluent suction trough rinse head)
4 Check pressure in-line [-425; -50] Control the pump suction in Tubing #10

5 Wait 300 ms To ensure diluent priming in rinse head

6 Init motor needle

Move Needle in its home position
7 Wait motor needle

8 Init motor rocker Move Rocker in its home position

9 Wait motor syringe

10 Pump off End of needle rinsing

11 Reset EV4

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 REF : M60-SM-000 Rev 02
E. DRAIN WASTE + ROCKER MOVES TO WBC BATH + NEEDLE DOWN IN WBC BATH
STEP ACTION COMMENT

1 Set EV7
Beginning of waste syringe draining
2 Goto pos syringe 1500

3 Goto pos rocker WBC bath

Rocker moves over WBC bath
4 Wait motor rocker

5 Goto pos needle rising position Needle moves down in rinsing position in WBC bath

6 Wait motor syringe End of waste syringe draining

F. EXTERNAL NEEDLE RINSING

STEP ACTION COMMENT

1 Set EV4

2 Syringe moves up External needle rinsing (EV4 + diluent syringe up)

3 Wait motor syringe

4 Wait motor needle Needle in rinsing position in WBC bath

5 Reset EV4

6 Wait 200 ms End of external needle rinsing

7 Reset EV7

G. WBC COUNTING BATH DRAINING + OPTIC TRANSFER LINE DRAINING + SAMPLE PROBE
IN DILUTION POSITION
STEP ACTION COMMENT

1 pump on

2 Set EV1+3+16+13 WBC bath draining + optic transfer line draining (pump + waste
3 Syringe moves down syringe)

4 Wait 700 ms

5 Pump off
End of optic transfer line draining
6 Reset EV13+16

7 Goto pos needle dilution position Needle moves down in WBC bath to dilution position

8 Check pressure waste [-600, -250] Control presence of diluent in WBC bath during bath draining

9 Wait motor syringe End of WBC bath draining

10 wait 1000 ms Stabilizing pressure in waste syringe

11 Check pressure waste [-150, +50] Control WBC bath empty

12 Reset EV1+3 End of WBC bath draining

13 Wait motor needle Needle in dilution position in WBC bath

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 REF : M60-SM-000 Rev 02
H. WASTE SYRINGE DRAINING
STEP ACTION COMMENT
1 Set EV7
2 Goto pos syringe 1300
Waste syringe draining
3 Wait motor syringe
4 wait 700 ms
5 Check pressure Waste [-50, +180] Control waste syringe empty

I. FIRST DILUTION (2ml OF DILUENT + 15.6µl OF BLOOD) IN WBC BATH

STEP ACTION COMMENT
1 Set EV4+6
2 Syringe moves up
1ml of diluent pushed in WBC bath through heating module
3 Wait motor syringe
4 Reset EV6
5 Set EV5
15.6µl of blood (already present inside the needle) + 1ml of
6 Syringe moves up
diluent pushed in WBC bath through sampling needle
7 Wait 300 ms
8 Reset EV7 End of waste syringe draining

9 Set EV8+17 Waste syringe + vacuum tank to the atmosphere (avoid over
)
10 Wait motor syringe
End of first dilution (2ml of diluent + 15.6µl of blood) + end of
11 wait 500 ms
waste syringe to the atmosphere
12 Reset EV4+5+8+17

J. FIRST BUBBLING IN WBC BATH + FIRST RINSING AT THE BACK OF THE APERTURES
STEP ACTION COMMENT

1 Set EV1+4+12+10

2 Goto pos syringe 0 First bubbling in WBC bath (EV1 + waste syringe moves up) +
Rinsing at the back of the apertures with diluent (EV4+12+10 +
3 Wait motor syringe diluent syringe moves up)
4 Reset EV1+4+12+10

K. PARTIAL RBC BATH DRAINING + DILUENT WITHDRAWAL ON WBC BATH

STEP ACTION COMMENT

1 Set EV2+4+6 Partial RBC bath draining (EV2 + waste syringe moves down)
Diluent withdrawal on WBC bath (EV4+6 + diluent syringe moves
2 Syringe moves down down) to avoid electric noise
3 Wait 300 ms
Note: Piston carriage against bottom E-clip of sampling
4 Reset EV4+6 piston
5 Wait motor syringe
End of partial RBC bath draining
6 Reset EV2

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 REF : M60-SM-000 Rev 02

L. FIRST DILUTION DRAWING

STEP ACTION COMMENT

1 Set EV8+17 Waste syringe + vacuum tank to the atmosphere

2 Syringe moves down First dilution drawing (25.6µl) inside the needle

3 Needle moves up
Needle moves up during the first dilution sampling
4 Wait motor needle

5 Wait motor syringe

End of first dilution drawing
6 Reset EV8+17

M. NEEDLE RINSING + ROCKER MOVES TO RBC BATH

STEP ACTION COMMENT

1 Pump on
Beginning of needle rinsing
2 Set EV4 Push diluent to rinse head (diluent syringe up + EV4)
3 Syringe moves up Pump on for diluent suction trough rinse head

4 Wait 300 ms

5 Check pressure in-line [-425, -50] Control the pump suction in Tubing#10

6 Needle moves up

7 Wait motor needle End of pump suction in Tubing#10 + needle up

8 Pump off

9 Set motor needle no power

10 Goto pos rocker RBC bath Rocker over RBC bath

11 Wait motor rocker

12 Goto pos needle rinsing position Needle moves to rinsing position in RBC bath

13 Wait motor syringe

End of needle rinsing
14 Reset EV4

O. LYSE DISPENSE (0.730ML) + NEEDLE RINSING (0.9ML) + WASTE SYRINGE DRAINING

STEP ACTION COMMENT

1 Set EV4+9+14+7

2 Wait 440 ms Needle external rinsing (EV4 + Diluent syringe up)

First Lyse dispense (EV9+14 + Lyse syringe up)
3 Syringe move up Waste syringe draining (EV7 + Waste syringe up)
4 Wait motor syringe

5 Wait motor needle Needle in rinsing position in RBC bath

6 Reset EV4 End of needle rinsing

7 Syringe moves up Second Lyse dispense (EV9+14 + Lyse syringe up)

8 Reset EV7 End of waste syringe draining

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 REF : M60-SM-000 Rev 02
9 Set EV8+17 Waste syringe + vacuum tank to the atmosphere

10 Wait motor syringe

End of lyse dispense
11 Reset EV17+8+9+14

P. SECOND BUBBLING IN WBC BATH

STEP ACTION COMMENT

1 Set EV1

2 Syringe moves up Second bubbling in WBC bath

3 Wait motor syringe

4 Reset EV1 End of bubbling in WBC bath

Q. RBC BATH DRAINING + NEEDLE DOWN IN DILUTION POSITION

STE
ACTION COMMENT
P
1 Pump on

2 Set EV2+3 RBC bath draining (pump + waste syringe)

3 Syringe moves down

4 Set EV9+14
Lyse withdrawal on WBC bath to avoid electric noise
5 Wait 140 ms

6 Reset EV9+14 End of Lyse withdrawal

7 Wait 150 ms RBC bath draining

8 Goto pos needle dilution position Needle moves to dilution position in RBC bath

9 Wait 250 ms RBC bath draining

Check pressure Waste [-450, -
10 Control presence of diluent in RBC bath
200]
11 Wait motor needle Needle in dilution position in RBC bath

12 Wait motor syringe

13 Pump off End of RBC bath draining

14 Wait 600 ms

15 Check pressure Waste [-150, +50] Control RBC bath empty

16 Reset EV2+3 End of RBC bath draining

R. WASTE SYRINGE DRAINING

STEP ACTION COMMENT

1 Set EV7

2 Goto pos syringe 1400

3 Wait motor syringe Waste syringe draining (EV7 + waste syringe up)

4 Wait 700 ms

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 REF : M60-SM-000 Rev 02
S. DILUTION RBC (25.6 µL FROM FIRST DILUTION + 2ML OF DILUENT)
STEP ACTION COMMENT

1 Set EV4+18

2 Syringe moves up Push diluent (1ml) to RBC bath

3 Wait motor syringe

4 Reset EV18

5 Set EV5
25.6µl from first dilution (already present inside the needle) +
6 Syringe moves up
1ml of diluent pushed in RBC bath through sampling needle
7 Wait motor syringe

8 Wait 500 ms
End of second dilution + end of waste syringe draining
9 Reset EV4+5+7

10 Check pressure Waste [-50, +180] Control waste syringe empty

T. OPTIC LED ON + MEASUREMENT CURRENT ON

STEP ACTION COMMENT

1 Led opt on Optic LED switched ON

Measurement current ON (60volts applied on RBC and WBC
2 Measurement current on
electrodes)

U. THIRD BUBBLING IN WBC BATH + FIRST BUBBLING IN RBC BATH + NEEDLE UP + SECOND
RINSING AT THE BACK OF THE APERTURES
STEP ACTION COMMENT

1 Goto pos needle rinsing position Needle moves to rinsing position

2 Set EV1+17

3 Syringe moves up
Bubbling in WBC bath + vacuum tank to the atmosphere
4 Wait motor syringe

5 Reset EV1

6 Set EV2+4+12+10

7 Goto pos syringe 0 First bubbling in RBC (EV2 + waste syringe up)
Second rinsing at the back of the apertures (EV4+12 + Diluent
8 Wait motor syringe syringe up)
9 Reset EV2+4+12+10

V. WASTE SYRINGE + VACUUM TANK TO THE ATMOSPHERE

STEP ACTION COMMENT

1 Set EV8
Waste syringe + vacuum tank to the atmosphere (avoid over
2 Wait 500 ms
pressure)
3 Reset EV8+17

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 REF : M60-SM-000 Rev 02
W. FIRST DILUTION TRANSFER TO OPTIC BENCH + DILUENT WITHDRAWAL ON RBC BATH
STEP ACTION COMMENT

1 Set EV13+16+4+18 First dilution transfer to optic bench (EV13+16 + waste syringe
down)
2 Syringe moves down Diluent withdrawal on RBC bath (EV4+18 + diluent syringe down)

3 Wait 600
Note: Tubing#33E is filled with first dilution
4 Reset EV4+18 End of diluent withdrawal on RBC bath

5 Wait motor syringe

End of first dilution transfer to optic bench
6 Reset EV13+16

7 Wait motor needle Needle in rinsing position

X. COUNTING VACUUM CREATION

STEP ACTION COMMENT

1 Set EV10+8
Counting vacuum creation
2 Syringe moves down

3 Wait motor syringe Note: First impedance counting is done by vacuum created in
waste syringe
4 Wait 500 ms

5 Check pressure Waste [-300, -100] Control the counting vacuum

6 Reset EV8 End of counting vacuum creation

Y. NEEDLE AND ROCKER MOTORS DESACTIVATION FOR COUNTING

STEP ACTION COMMENT

1 Set motor rocker no power Needle and rocker motors deactivation during counting (avoid
2 Set motor needle no power electrical noise)

Z. FIRST COUNTING OF 3 SECONDS (IMPEDANCE ONLY)

STEP ACTION COMMENT

1 Start count 3
First counting on both aperture for three seconds
2 Wait 3200 ms
Control that loss of vacuum due to first counting does not
3 Check pressure Waste 5%
exceed 5%
4 Wait 100 ms

AA. BACK FLUSH WITH DILUENT IN BOTH APERTURES

STEP ACTION COMMENT

1 Set EV4+12

2 Syringe moves up
Diluent back flush into apertures to avoid potential clogging
3 Reset EV10

4 Wait 125 ms

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5 Set EV10

6 Wait motor syringe

7 Reset EV4 End of diluent backflush

8 Wait 200 ms

9 Reset EV12

BB. COUNTING VACUUM REGENERATION

STEP ACTION COMMENT

1 Set EV8 Counting vacuum regeneration to compensate the loss of vacuum

2 Syringe moves down from first counting

3 Wait motor syringe Note: Second Impedance counting is done by vacuum created
4 Wait 500 ms in vacuum tank

5 Check pressure Waste [-300, -100] Control the counting vacuum

6 Reset EV8 End of counting vacuum regeneration

CC. SAMPLE INJECTION + DILUENT SHEATH IN FLOWCELL

STEP ACTION COMMENT

1 Set EV4+12+15+9+7 Sample injection in flow cell (EV9 + lyse syringe up)
Active sheath (EV4+12+15 + diluent syringe up)
2 Syringe moves up Waste syringe draining
Stabilization of flow inside the flow cell
3 Wait 1200 ms

DD. SART SECOND COUNTING OF 7 SECONDS (IMPEDANCE) + START OPTIC

MEASUREMENTS FOR 8 SECONDS
STEP ACTION COMMENT

1 Start count 7s + 8s Start impedance counting (7seconds) + optic counting (8seconds)

2 Wait 7300 ms End of second impedance counting

EE. PRIME THE BACK OF THE APERTURES WITH CLEANER + END OF OPTIC MEASUREMENTS
STEP ACTION COMMENT

1 Set EV11 Prime cleaner at the back of the apertures thanks to the
2 Wait 650 ms residual vacuum present in vacuum tank

3 Reset EV10+11 End of cleaner priming

4 Wait 350 ms
End of optic counting
5 Led optic off

6 Measurement current off Switched off measurement current

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FF. PRIME LYSE ON WBC BATH + STOP SAMPLE INJECTION+DILUENT SHEATH IN FLOWCELL
STEP ACTION COMMENT

1 Set EV14
Prime lyse on WBC bath (lyse syringe still moving up)
2 Wait 300 ms

3 Reset EV9+14 Stop prime lyse on WBC bath

4 Wait motor syringe Rinse the flow cell during the Wait motor syringe then stop
5 Reset EV4+12+15+7 sample injection + diluent sheath in flowcell

6 Goto pos needle rinsing Position Needle moves to rinsing position

GG.WBC BATH DRAINING + OPIC TRANSFER LINE DRAINING

STEP ACTION COMMENT

1 Pump on

2 Set EV1+3+13+16 WBC bath + optic transfer line draining (using pump + waste
3 Syringe moves down syringe vacuum)

4 Wait motor syringe

5 Reset EV13+16
End of optic transfer line draining
6 Pump off

7 Wait 100 ms
End of WBC bath draining
8 Reset EV1+3

9 Wait motor needle Needle in rinsing position

HH. WBC BATH FILLING WITH DILUENT + WASTE SYRINGE DRAINING + BACKFLUSH
IN THE APERTURES WITH CLEANER
STEP ACTION COMMENT

1 Set EV4+6+7
WBC bath filling with diluent through heating module
2 Syringe moves up
Waste syringe draining (EV7 + Waste syringe up)
3 Wait motor syringe

4 Reset EV6 End of WBC bath filling

5 Set EV12

6 Syringe moves up Backflush into apertures using cleaner

7 Wait motor syringe

8 Reset EV4+7+12 End of backflush + end of waste syringe draining

II. RBC BATH DRAINING + PRIME OPTIC TRANSFER LINE WITH DILUENT
STEP ACTION COMMENT

1 Pump on

2 Set EV3+2 RBC bath draining (using pump + waste syringe vacuum)

3 Syringe moves down

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4 Wait 200 ms

5 Set EV13+16
Rinse optic transfer line with diluent (from WBC bath)
6 Wait 750 ms

7 Reset EV13+16 End of optic transfer priming

8 Wait motor syringe

9 Reset EV3
End of RBC bath draining
10 Pump off

11 Wait 500 ms
Control RBC bath empty
12 Check pressure Waste [-150, +100] Note: This check pressure control also the correct operation
of the valve #12 (from previous step BB)
13 Reset EV2

JJ. RBC BATH FILLING WITH DILUENT + WASTE SYRINGE DRAINING + NEEDLE DRYING
STEP ACTION COMMENT

1 Set EV3+4+7
Push diluent through rinse head to fill RBC bath by the external
2 Syringe moves up of the needle (EV3+4 + diluent syringe up)
Waste syringe draining (EV7 + waste syringe up)
3 Wait motor syringe

4 Reset EV3
Needle moves to home position during drying + Tubing#10
5 Pump on
draining
6 Needle moves up

7 Set EV18
Push diluent to RBC bath
8 Syringe moves up

9 Set EV10+17 To suppress over pressure on apertures due to backflush with

10 Wait 300 cleaner

11 Check pressure in line [-425, -50] Check the correct operation of the valve #18

12 Send result Result is sent for computation and display

13 Wait motor needle

Needle up + needle motor disactivated (for rocker movement)
14 Set motor needle no power

15 Wait motor syringe

End of RBC bath filling
16 Reset EV4+10+17+18

KK. NEEDLE + ROCKER+ SYRINGE INTIALIZATION

STEP ACTION COMMENT

1 Syringe moves up
Syringe moves to home position
2 Wait 300 ms

3 Goto pos rocker, Sampling Position

Rocker moves to sampling position
4 Wait motor rocker

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 REF : M60-SM-000 Rev 02
5 Needle moves to sampling position Needle moves to sampling position if another cycle is triggered

6 Wait motor syringe Syringe to home position

7 Set EV8+17 Waste syringe to atmosphere

8 Wait motor needle Needle in sampling position

9 Pump off End of needle drying + end of Tubing#10 draining

10 Wait 300 ms End of waste syringe drying + end of Waste syringe to the
11 Reset EV7+8+17 atmosphere

Page 50/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

5. OUTPUT FORMAT
5.1 connection types

RS-232 cable
Mythic 60 is equipped with DB9 MALE connector.
In order to connect it to LIS host CROSSED cable is required.
There is only 3 signals necessary: RXD,TXD,GND

NET (Ethernet cable)

This connection type requires standard ethernet cable terminated with RJ45 plug.

5.2 SEIS M60 Format C2

It is a specific format designed by C2 to transfer data to an external system. To ease the use of these data,
the chosen format is text oriented and is compatible with CSV format. The identifiers have to be in English.

Generic principles

The Mythic 60 begins its frame with the following header:

M60;W;X;;Y;[CR]
Where :
W is the Free instrument number (2 chars maximum).
X is the Free serial number (13 chars).
Y is the user login, UTF8 coded.

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 REF : M60-SM-000 Rev 02

The decimal separator is the dot.

The fields separator is the semicolon.
The lines separator and the end of frame indicator are the carriage return.
The atomic frames are :
• Results sending request with results sending
• Sending of calibration packet with calibration results
All the frames lines have to be emitted ( if there is no information, the parameter is left empty ).
All the identifier keywords (of frame or of parameter ) are in upper-case.
The following fields ( SID,PID, ID, blood type, operator, lot numbers ) are UTF8 encoded.
The DATE format is set to DD/MM/YYYY, time is set to HH:MM:SS in 24 hour mode.

Maximum fields length for fields shared among different frames

OPERATOR 10 chars
USER 10
LOT (CALI or QC) 8
SID 16
PID 16
ID 20

Unit
UNIT;u[CR]

Unit code value u Unit system

1 USA = standard
4 S.I.
7 S.I. MOD
3 OTHER

Page 52/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02
FRAMES
RESULT SENDING
The M60 sends the results to the host.

M60 Host

M60;1;510207-000451;;BILL[CR]
RESULT[CR] result frame
Analysis result sending

Analysis result sending

M60;1;510000-000000;;A;
RESULT
DATE;14/06/2017
TIME;10:03:57
MODE;NORMAL
UNIT;1
SEQ;63;1
SID;4
PID;
ID;
TEST;CD
TYPE;DIF WB
BIRTH;
Gender;0
Group;0
Doctor;
PATIENT COMMENT;
SN;510000-000000
OPERATOR;ADMIN
PREL;OT
ALTITUDE;N
CYCLE;N
WBC;8.4 ; ; ; ;2.0 ;4.0 ;12.0 ;15.0 ;
LYMP;1.1 ; ;L; ;15.0;25.0;50.0;55.0;
MONP;0.8 ; ;L; ;1.0 ;2.0 ;10.0;12.0;
NEUP;97.3; ;H; ;45.0;50.0;80.0;85.0;
EOSP;0.8 ; ; ; ;0.0 ;0.0 ;5.0 ;8.0 ;
BASP;0.0 ; ; ; ;0.0 ;0.0 ;2.0 ;5.0 ;
IMMP;0.6 ; ; ; ;0.0 ;0.0 ;100.0;100.0;

Page 53/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02
ALYP;21.2; ; ; ;0.0 ;0.0 ;100.0;100.0;
LYM;0.1 ; ;L; ;0.7 ;1.0 ;5.0 ;5.5 ;
MON;0.1 ; ; ; ;0.0 ;0.1 ;1.0 ;1.1 ;
NEU;8.2 ; ;h; ;1.5 ;2.0 ;8.0 ;9.0 ;
EOS;0.1 ; ; ; ;0.0 ;0.0 ;0.4 ;0.6 ;
BAS;0.0 ; ; ; ;0.0 ;0.0 ;0.2 ;0.3 ;
IMM;0.1 ; ; ; ;0.0 ;0.0 ;150.0;150.0;
ALY;0.0 ; ; ; ;0.0 ;0.0 ;150.0;150.0;
RBC;4.50 ; ; ; ;2.50 ;4.00 ;6.20 ;7.00 ;
HGB;13.0; ; ; ;8.5 ;11.0;17.0;19.0;
HCT;36.8; ; ; ;25.0;35.0;55.0;60.0;
MCV;81.8 ; ; ; ;70.0 ;80.0 ;100.0;120.0;
MCH;28.9; ; ; ;25.0;26.0;34.0;35.0;
MCHC;35.3; ; ; ;28.0;31.0;35.5;37.0;
RDW-CV;11.2; ; ; ;7.0 ;10.0;16.0;25.0;
RDW-SD;33.6 ; ;l; ;25.9 ;37.0 ;46.0 ;71.9 ;
PLT;294 ; ; ; ;70 ;150 ;400 ;500 ;
MPV;12.7; ;H; ;6.0 ;7.0 ;11.0;12.5;
PCT;0.374; ; ; ;0.100;0.200;0.500;0.600;
PDW;15.7; ; ; ;8.0 ;10.0;18.0;25.0;
PLCR;22.0; ; ; ;8.0 ;12.0;42.0;60.9;
PLCC;65 ; ; ; ;6 ;18 ;168 ;304 ;
WBC
CURVE;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;1;2;2;2;2;2;2;2;2;2;2;2;3;5;9;14;20;26;35;47;60;74;90;111;136;160;
182;203;222;236;242;244;243;238;227;212;195;176;158;137;114;96;81;69;57;45;34;25;19;14;11;8;5;4;3;2;2;
2;2;2;2;2;2;2;2;2;2;2;2;2;2;3;3;3;3;3;3;3;3;3;3;3;3;3;3;3;3;3;2;1;1;1;1;1;1;1;0;0;0;0;0;0;0;0;0;0;0;0;0;0;
WBC THRESHOLDS;32;120;0;
RBC
CURVE;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;1;1;1;2;3;5;7;10;14;19;26;35;48;63;82;106;132;157;178;200;221;2
36;244;243;233;218;202;183;162;140;119;99;79;62;49;39;31;24;19;16;13;11;9;7;6;5;4;4;4;4;4;4;4;4;4;4;5;6;6;
6;6;6;6;6;6;6;6;5;5;5;5;4;4;3;3;2;2;2;2;1;1;1;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;
RBC THRESHOLDS;32;62
PLT
CURVE;0;0;0;0;0;2;5;11;20;30;40;52;65;75;85;95;103;108;114;117;120;121;124;124;126;126;124;123;121;118;11
5;113;110;105;103;98;94;90;85;80;75;70;65;61;58;55;52;50;48;45;44;41;40;37;34;32;30;28;27;25;24;22;21;
20;18;17;15;14;12;11;10;8;7;7;5;5;4;4;4;4;4;4;4;4;4;4;4;4;4;4;4;4;4;4;4;4;4;4;4;4;4;4;4;2;2;2;2;2;2;2;2;4;4;4;4;
4;4;4;4;4;4;4;4;4;4;5;5;5;
PLT THRESHOLDS;100
ALARMS;NH ;QC-F;
INTERPRETIVE_WBC;LYM<;NEU>;
INTERPRETIVE_RBC;
INTERPRETIVE_PLT;MACROP;
COMMENT;ALY, IMM, PLCR, PCT, PLCC and PDW are for Research Use Only;
DYNAMIC_MATRIX;
Z982;46;Z3;46;46;Z3;47;47;Z3;46;Z2;46;Zc;46;Z24;46;Z3c;46;46;Z6;47;51;47;46;0;47;46;46;Z4;46;Z3;46;
0;46;Zb;46;Z1e;46;Z39;50;Z3;50;0;50;50;Z4;50;Z5;46;47;Za;46;Z8;46;Z62;50;0;50;Z6;47;46;Z5;46;Z14;46
;Z11;46;Z44;50;Z5;51;Z3;46;Z5;46;Z5;46;Z5;46;
;Z2;46;Z5e;50;Z2;50;Z4;50;50;Z3;46;Z2;46;Z2;46;0;46;Z6c;50;Z3;50;0;50;50;50;50;Z4;46;Z4;46;Z5;46;Z
6c;50;0;50;Z2;46;Z3;46;Z75;51;Z4;50;Z5;46;46;Z7;47;Zd;46;Z5a;50;Z16;46;Z6c;50;0;50;51;Z12;46;Z66;50
;Z5;50;0;50;0;46;Z3;47;Z5;46;Z4;46;Z69;50;Z8;47;

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 REF : M60-SM-000 Rev 02
;Z2;46;Z1e;46;Z3;46;Z55;50;Z10;46;Z6e;51;Z5;46;Z3;46;Z76;50;Z7;46;Z21;46;Z53;50;0;50;Zc0;46;Z4d;46
;Z6b;a;0;a;Z4;a;Za;46;Z34;46;Z3a;a;Zb;a;Z38;46;Z3c;a;Z2;a;Z2;a;Z3;a;Z11;46;Z2e;46;Z38;a;Zc;a;Z19;1e;Zb;
46;Z15;1e;Z12;28;Z29;a;Z2;b;0;b;Z26;1e;Zf;1e;
;0;1e;Z5;1e;Z37;a;Z5;a;Z34;1e;Z48;a;Z29;1e;Za;1e;Z2;1e;Z2;1e;Z48;a;Z23;1e;Z9;1e;Z25;28;Z14;a;Z21;1e;Z20
;1e;Z2;1e;1e;Z5;1f;1e;Z2f;a;Z18;a;Z18;1e;Z15;1e;0;1e;Z9;1e;0;1e;Z6e;1e;0;1e;Z3;1f;0;1f;0;1e;Z8;1e;Z62;1e;Za;1
e;1e;Zf;1e;1e;Z10;28;Z6;28;Z4d;1e;
;Z2;1e;Z3;1e;1e;1e;1e;1f;Z4;1e;Z2;1e;1e;1e;Z6;1e;Z8;1e;Z5b;1e;1e;Z2;1f;Z2;1e;1e;0;1f;1e;Z2;1e;1e;1f;1e;1e;Z5;1
f;Z12;28;Z2e;a;Z25;1e;Z2;1e;1f;1f;Z2;1f;1f;1f;1e;1e;0;1f;0;1e;1f;1f;1f;0;1e;Z2;1e;Z64;1e;1e;0;1e;1e;1e;1e;1e;1e;
1e;0;1f;1e;1e;1f;1f;
;Z5;1e;1e;Zf;1e;Z3b;a;Z18;1e;Z3;1f;0;1f;1e;0;1f;1f;1e;1f;0;1e;1f;1f;1f;1f;1f;1f;1e;1e;1f;1e;0;1e;1e;0;1e;1e;0;1e;0;
1e;1e;Z6;1e;Z4;28;Z3c;1e;Zb;1e;0;1e;Z4;1e;Z5;1e;0;1e;0;1e;0;1e;1f;1f;1f;1e;1f;1f;1e;1e;1f;0;1f;1f;0;1f;1e;Z2;1e;
1e;Zb;1e;Z13;28;
;Z28;a;Z16;1e;Z4;1f;1f;0;1f;1f;1f;1f;1f;0;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;0;1f;1f;1e;1f;1e;1e;Z2;1f;1f;Z2;1e;Z4
;1e;Ze;28;Z45;1e;Z2;1e;Z2;1e;1f;1f;1f;1e;1e;0;1e;1e;1f;1f;1f;1f;1f;1e;1f;1e;0;1f;1e;1e;1e;1e;1f;Z2;1e;1e;Z2;1e;Z3
;1e;Z5;1e;Z7;28;
;0;29;Z6;28;Z1b;b;Z22;1e;0;1e;Z2;1e;1f;1e;1e;0;1f;1f;1f;1f;1f;1f;1e;1f;1f;1f;1f;1f;1e;1f;1f;1f;Z2;1f;1f;1f;1e;0;1e
;0;1e;0;1e;Z2;1e;Z6;1e;Z2b;a;Z21;1e;Z5;1e;0;1e;0;1f;1f;1f;1f;1f;1e;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;0;1f;1e;1e;1
e;1e;1f;1f;1f;
;0;1e;0;1e;0;1f;Ze;28;Z1b;a;Z4;a;Z4;a;Za;a;Z19;1e;1e;1e;1e;1f;1f;Z2;1f;1e;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1
f;1f;1f;1f;1f;1f;1f;1f;1e;1f;Z2;1e;Z2;1e;1e;Z7;1e;Z5;28;Z2;28;Z2;28;Z6;28;Z16;a;Z6;a;Z9;a;Z9;1e;Za;1e;1f;0;1
f;1f;Z2;1e;1f;1f;1f;
;1f;1f;1f;1f;1f;1e;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1e;1e;1f;1e;Z2;1f;0;1e;1e;1e;1f;1e;1e;Z4;1e;Z2;1e;Z7;28;Z1b;
a;Za;b;0;a;Zd;1e;Z9;1e;Z6;1e;1f;0;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;0;1f;1f;
0;1e;1e;1f;
;Z2;1f;1f;1f;Z9;28;28;Z4;28;0;28;Z15;a;Z6;a;a;a;Z1f;1f;Z3;1e;Z2;1e;0;1f;1f;1f;1e;1f;0;1f;1f;1f;1f;1f;1f;1f;1f;1f
;1f;1f;1f;1f;1f;1f;1f;1f;1e;1f;1f;1f;1f;1f;1f;1e;1f;Z2;1e;Z2;1e;1e;1e;1e;Z3;1e;Z33;a;Z11;1e;Z4;1e;1f;Z3;1e;0;1f;1f
;1f;1f;1f;1e;1f;
;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1e;0;1f;1e;Z3;1e;0;1e;Z4;1e;1e;0;28;28;
Ze;28;Z12;a;0;a;Zc;b;Z17;1f;0;1e;1e;Z2;1e;1f;1f;1f;1f;1f;1f;0;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;
1f;1f;1e;
;1f;1f;1f;Z2;1e;1f;Z3;1e;1e;0;1f;1e;Z2;1e;Z6;28;Z24;a;Z1c;1e;Z2;1f;1e;1e;0;1e;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;
1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;0;1f;1e;Z2;1f;Z2;1e;1e;Z3;1e;1f;1e;Z4;28;Z2;29;Z2a;a;Z2;a;a;Z16;1
e;1e;Z6;1f;0;1f;
;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1e;1f;1f;1f;1f;0;1e;1f;0;1e;Z3;1e;1e;Z4;1e;Z9
;28;Z1c;a;0;b;a;a;0;a;a;a;b;a;Z11;1e;1e;Z4;1e;1e;0;1f;Z3;1f;1e;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;
1f;1f;1f;
;1f;1e;1f;1f;1f;1f;1f;1f;1f;1f;0;1f;1e;1f;1f;0;1f;1e;0;1e;1e;Z2;1f;28;Z2;28;Z28;14;14;0;a;Z12;1e;Z4;1e;Z5;1e;1e;1
e;1e;1f;1f;1f;1e;1f;1f;1f;1f;1f;1f;1f;1e;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1e;1e;1f;1f;1e;0;1e;1e;Z3;1
e;1f;Z11;28;
;28;Z19;14;Z3;15;Z2;14;14;Za;1e;Ze;1e;1e;1e;Z3;1e;1e;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1
f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;0;1e;0;1f;1f;1e;1e;Z7;1e;Z29;14;Z2;14;Z2;14;14;Z10;1e;Z8;1e;1e;0;1f;1e;1f;1f;1
f;1e;1f;1f;1f;1f;
;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1e;1f;1f;1f;1f;1e;Z3;1e;Z1c;28;Z18;14;0;14;Z16;
1e;Z2;1e;1e;1f;1e;1f;1e;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;
1f;1f;
;1f;0;1f;1f;1e;1e;Z4;1e;Z30;14;Z19;1e;Z2;1e;1e;1e;1e;0;1e;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1
f;1f;1f;1f;1f;1f;1f;1e;1f;1e;1f;1f;1f;1f;1e;0;1e;1e;1f;Z2;1e;Z7;28;Z26;14;Za;14;0;15;Z3;1e;Z13;1f;0;1e;1f;1f;1f;0
;1f;1f;1f;1f;
;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1e;1f;Z2;1e;0;1e;Z3;1e;Z16;28;Z15;14;Z21;1
e;Z3;1e;1e;0;1e;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1e;1f;1e;1f;0;1e
;1f;Z2;1e;

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 REF : M60-SM-000 Rev 02
;Z3;1e;Z31;14;Z17;1e;Z3;1e;1e;0;1e;1e;1e;0;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f
;1f;1f;1f;1f;1f;1f;1f;1f;1e;0;1f;1f;Z7;1e;Z30;14;Z6;14;Z13;1e;Z3;1e;1e;1e;0;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;
1f;1f;1f;1f;
;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1e;1f;0;1f;0;1f;1e;Z4;1e;1e;Z2;1e;Z2e;14;14;0;14;Z4;14;Z6;1e;Z5;1e;Z2;1e;Z4;1e
;1e;Z2;1f;Z4;1e;1f;1e;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1
e;0;1e;Z5;1e;
;Z3;28;Z32;14;Zc;1e;Z6;1e;1e;1e;Z3;1e;0;1f;1f;1f;Z2;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;
1f;1f;1f;1f;1e;1f;1f;1e;1e;1e;1e;1e;1e;1f;0;1f;1e;Z38;14;Z12;1e;Z2;1e;1e;1f;1e;1f;1f;0;1e;0;1e;1f;1f;1f;1f;0;1f;1f;1
f;1f;1f;1f;
;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1e;1f;1e;1f;0;1f;Z3;1e;Z4;1e;28;Z46;1f;Z7;1e;0;1f;1f;Z2;1e;0
;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;0;1f;1f;1f;1f;1f;1f;1f;1e;1e;Z53;1e;Z4;1f;Z2;1e;0;1e;1f;1f;1e;1
f;1f;1f;1f;
;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1e;1f;1f;1e;1f;1f;1e;0;1f;1f;1e;1f;Z4c;1f;Z7;1e;Z3;1f;1e;Z2;1e;0;1e;
1e;1f;1e;1f;1f;1f;1f;1e;1f;1f;1f;1f;1f;1f;1e;1f;1f;1f;1f;0;1e;1e;1f;1f;0;1f;1f;Z2;1f;1e;0;1e;Z4;1e;Z4a;1e;Z3;1f;1e;1
e;1e;Z3;1e;
;Z3;1e;1e;1f;1f;1f;1f;1f;1e;1e;1f;1f;0;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1e;1f;1f;0;1f;0;1e;1f;1e;Z2;1f;1e;Z3;1e;Z3
b;14;Z11;1e;1e;0;1e;1f;Z3;1f;1e;1e;1e;1e;1e;Z2;1f;1e;1f;1f;1e;1f;1f;1f;1f;1f;1e;1e;1f;1f;1f;1e;1f;1e;1e;1f;0;1f;0;1f;
1f;0;1f;
;1e;0;1e;Za;28;Z3;28;Z41;1e;0;1e;Z5;1e;1e;1e;1e;1e;1f;1e;1f;1f;0;1f;0;1e;1f;1f;1f;1f;1f;1f;1f;1f;1f;1f;1e;1f;1e;1f;
0;1e;1e;1f;1e;1f;0;1f;1e;1e;1e;1e;1e;Z10;28;Z3d;1e;Z2;1e;0;1e;1e;Z4;1e;1e;0;1e;1e;1e;Z2;1e;1e;1f;1e;1f;1e;1f;1e;0
;1f;0;1f;1f;1e;
;1e;1f;0;1e;0;1f;1e;1f;0;1e;0;1e;1e;1f;1e;1e;Z1e;28;Z20;14;Z15;1f;Z5;1f;0;1f;1f;1f;1e;1e;0;1f;0;1f;1e;1f;1e;1f;1f;1
f;1f;1f;1e;1f;1e;1f;1e;1f;1e;0;1f;Z4;1e;0;1e;0;1e;1e;Z2;1e;Z39;14;Z14;1e;Z2;1e;0;1e;Z3;1e;1f;1f;0;1f;1e;1e;1e;1e;
0;1f;1e;1e;1f;
;Z3;1f;1f;1f;Z2;1f;1e;Z2;1e;Z7;1e;Z4;1e;Z6;1e;Z40;14;Z4;1e;Z7;1e;0;1f;1f;1e;Z2;1f;1e;1e;1e;1f;1f;1f;1e;Z2;1f;1
f;1f;1e;1f;1e;1f;Z2;1f;0;1f;1e;0;1e;1e;1e;Z6;1e;Z51;1e;1e;Z6;1e;1e;Z2;1e;0;1e;0;1f;Z3;1f;0;1f;Z3;1e;1e;0;1e;1f;1e
;0;1e;0;1f;1e;Z2;1e;
;Z5a;1e;Z5;1e;0;1f;Z5;1e;1f;1e;Z2;1e;1e;0;1e;1e;1e;1e;1e;Z2;1e;1e;Z2;1f;0;1e;Z6;1e;0;1e;Z5;1e;Z4d;1e;Z5;1f;0;1
e;Z3;1e;1e;1f;1e;1f;Z5;1e;Z2;1e;0;1f;1e;Z4;1e;Z2a;28;Z30;1f;Z9;1e;Z2;1f;Z2;1e;1e;0;1f;Z2;1e;1e;1f;1f;1e;0;1e;
Z4;1f;Z2;1e;Z3;1e;Z3;1e;
;Z4e;14;Z4;1e;0;1f;Z4;1e;1e;Za;1e;0;1e;Z5;1f;0;1e;0;1e;1f;Z1b;28;Z4a;1e;0;1e;Z4;1e;Z6;1e;0;1e;0;1e;Z4;1e;Z2;1
e;0;1e;1e;Z2;1e;Z55;14;Zc;1e;Z16;1e;Z3;1e;Z3;1e;Zf;28;Z49;1e;Z3;1e;Z7;1e;1e;1e;Z6;1f;1f;Z7;1e;Z4;1f;Z9;1e;
Z58;1e;1e;1e;Z4;1e;Zc;1e;Z5d;14;
;Z18;1f;Za;1e;Z3;1e;0;1e;1e;Z3;1e;Z8;1e;Z47;14;Z7;1e;Zd;1e;Z38;28;Z31;14;Za;1e;Z3;1e;Z1d;1e;Z5a;1e;Ze;1e;0
;1e;Ze;1e;Z67;1e;Z25;1e;Z46;14;Zd;1e;Z3;1e;1e;Z63;a;Z2;14;Z5;14;Z1e;1e;Z8;1e;Z5b;14;Z85;14;Zf6;14;Z5;14;
Z5;1e;Z8d;3c;Zb5;28;Z5a;3c;Z16;3c;
;Z38;3c;Z2e;3c;Zd7;3c;Z24;3c;Z25;3c;Z26;3c;Z14;3c;Z27;3c;Z5f;3c;Z6f;3c;Zb0;3c;Z51;3c;Z105;3c;Zc6;3c;
3c;Z82;3c;Zb3;3c;Zf;3c;Zb;3c;Z4;3c;Z11;3c;Z11;3c;3c;3c;3d;0;3c;0;3c;0;3c;0;3c;3c;;T
END_RESULT;56134

Haematological parameter format

Id ; value ; flag A ; flag B ; low panic ;low ; high ;high panic [CR]

WBC; 11.0 ;A ;B ; 2.0 ; 4.0 ; 11.0 ; 15.0 [CR]

rank Fields Valeurs possibles

1 Parameter id WBC
2 Parameter value in the selected unit 5.84
+ + + + + (5 plus) if overrange
----- (5 dashes) if unvalid result

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 REF : M60-SM-000 Rev 02
3 Flag A • Nothing
• ‘*’ if result is rejected (counting or measure
rejection) or invalidating fault.
• ‘!’ : suspicion
• + over linearity range
4 Flag B Nothing
‘L’ Result < panic value
‘l’ Result < normal value
‘h’ Result > normal value
‘H’ Result > panic value
5 Low Panic value 2.0
6 Low value 4.0
7 High value 6.0
8 High panic value 8.0

Alarms list
L1
L5
R1
R2
P1
P2
P3
RL
ALY
HL
NL
NH
IMM
S-UP FAIL
QC-ND
QC-F
INS-T
O-DF
WBC-BAL
W-CL
R-CL

WBC interpretive message

NO_INTERPRETATION
LEU>
LEU<
LYM>
LYM<
MON>
NEU>
NEU<
EOS>
BAS>
LIC

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 REF : M60-SM-000 Rev 02
ALY
ERYB
PLTAGGR
RBC interpretive message

NO_INTERPRETATION
ANE
ERY>
ANIS>
MACRO
MICRO
COLDAGG
HYPOCR
MACR>
MICR>

PLT interpretive report

NO_INTERPRETATION
THR>
THR<
MACROP
MICROP
CELLD
SCHIZ

DYNAMIC MATRIX; [CR] Squeezed DIF matrix

m;m;m;m;m;m;m;m;…;m;m;m;m; [CR]
m;m;m;m;m;m;m;m;…;m;m;m;m; [CR] Variable lines number.
…………
m;m;m;m;m;m;m;m;m;m;m;T[CR]
[CR]

m : compressed ASCII field, 1 to 4 characters.

Each line size is below 250 characters. Lines number is variable.

Dynamic clusters matrices

dynamic clustering matrices are matrices with which thresholds are evaluated through the use of a special
algorithm. This algorithm intends to locate white cells population pretty automatically leading to non-fixed
thresholds. In this case, each cell of the scartergram is determined to be from a cell family or another.
The identification of the cells is given by the value found in the cell:

Code Category
10 LYM with only
one cell
11 LYM
20 MON with
only one cell

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 REF : M60-SM-000 Rev 02
21 MON
30 NEU with only
one cell
31 NEU
40 EOS with only
one cell
41 EOS
50 BAS with only
one cell
51 BAS
60 IMM with only
one cell
61 IMM
70 D1 area with
only one cell
71 D1 area
80 Low LYM with
only one cell
81 Low LYM

Compression

Those matrices have a great footprint that needs to be reduced so that we don’t end up with a fat file..
To proceed, we use a RLE-based compression algorithm headed by a table of content.

Here it goes; we start with an array of 16KB of matrix data, 128x128 bytes to compress.

This is a two step compression:

1°) we run through the 16384 bytes to fill a list with encountered byte values. List size shall not exceed number
of different values stated by the variable threshold algorithm. Once you get your list (look-up table) of
different values (no doubloons !) you can start compressing..

2°) we run through the 16384 bytes again, and this time, you count how many times you get a given byte value.
You then store this counter value with the index (that refers to the byte value in the first list) of that
encountered byte value. Counter and index are converted into base-20 so that it takes less room in the ending
file. Each of that couples (counter, index) are semicolon separated.

Below, compression algo:

QString UTIL::SqueezeMoreMatrix( u_char *pucSrcMatrix, size_t size)

{
QString strData;
uint uiCurrByte = 0;
QList<uchar> lstLUT;
bool bOk = false;

//build LUT - what are the different encountered chars ?

for(uint iByte = 0; iByte < size; iByte++)
if(!lstLUT.contains(*(pucSrcMatrix + iByte)))
lstLUT.append(*(pucSrcMatrix + iByte));

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while(uiCurrByte < size)

{

uchar ucChar = *(pucSrcMatrix + uiCurrByte);

uint uiCurrOffset = 0;

//how many of the same chars ?

while((ucChar == *(pucSrcMatrix + uiCurrByte + uiCurrOffset)) && (uiCurrOffset <
QString(COMP_MAX_OCC).toUInt(&bOk, COMP_BASE_CONV)))
uiCurrOffset++;

//write down that char using index in LUT

if(uiCurrOffset == 1)
strData += QString::number(lstLUT.indexOf(ucChar), COMP_BASE_CONV) + ";";
else
strData += QString::number(uiCurrOffset, COMP_BASE_CONV) +
QString::number(lstLUT.indexOf(ucChar), COMP_BASE_CONV) + ";";

uiCurrByte += uiCurrOffset;
uiCurrOffset = 0;

//convert each byte value into base-20, more room !

QString strLUT;
foreach(uchar val, lstLUT)
strLUT += QString::number(val, COMP_BASE_CONV) + ";";

strLUT.chop(1);

strLUT.append("|");

//prepend this LUT to compressed data.

return strLUT + strData;

As of a RLE-based compression, the less events in the matrix, the more compressed result, you get, ranging
from 109 bytes (for a quite empty matrix) to 8KB (for the most complex ones). Average size would be around
4KB (against 16KB).

You finally get something like this:

In red: LUT data separated by a pipe from effective data. Here, 16 different byte values were encountered
in that matrix.
In green: An example of RLE couple (61H,0) which means 61H=>1569 times the byte value we find at index (0
=> 0), which is actually 0. Please note that if a byte value is encountered just one time, we don’t prepend the
counter (example in blue, we just encountered the byte value at index 1 and which is 46 => 70.

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 REF : M60-SM-000 Rev 02
0;46;47;51;50;A;B;1F;28;1E;32;14;33;15;3C;3D|61H0;1;30;21;0;21;0;1;F0;1;4I0;1;20;1;0;2;0;1;2;
0;21;20;1;5G0;1;20;1;0;1;2;21;0;1;5H0;1;20;1;20;2;0;1;20;1;40;1;5A0;21;0;1;0;1;20;21;600;1;3;
50;1;0;1;680;1;5H0;34;60;2;0;21;80;1;40;1;4J0;4;70;4;0;1;30;1;D0;1;500;34;20;24;60;1;5E0;4;0;
4;0;4;40;4;5J0;4;40;4;5;0;3;20;24;40;5;180;1;470;5;0;6;20;5;5J0;5;20;5;6C0;5;40;5;90;1;530;25
;30;5;660;6;50;5;0;25;5C0;5;0;5;20;5;20;5;30;25;0;5;50;1;5E0;6;20;5;20;5;20;5;5H0;5;26;0;5;0;5
;0;5;60;1;5D0;5;0;5;60;25;50;1;I0;1;4A0;6;20;6;5;0;6;5;30;6;20;5;A0;1;520;5;30;6;5;6;35;5G0;2
5;30;6;5;26;0;25;0;5;E0;1;530;5;6;25;6;0;5;0;5;5G0;25;6;5;6;5;46;0;6;50;5;0;1;560;5;30;6;5;0;5
6;0;6;1F0;1;3F0;5;30;5;0;D6;E0;1;4J0;6;0;A6;5;6;5D0;5;E6;35;0;25;0;5;560;25;D6;5;5A0;5;0;25;
0;A6;5;6;20;6;60;7;240;8;2H0;5;0;D6;0;5;0;5;5A0;5;C6;5;26;5D0;5;C6;0;6;5;0;5;580;25;6;0;D6;5;
30;25;1A0;8;3F0;35;0;A6;0;26;580;5;40;25;B6;20;5;5D0;B6;5;6;5;20;5;1C0;8;2C0;8;150;C6;5;6;30
;5;50;39;530;86;5;26;20;5;0;5;3J0;8;1C0;56;5;46;0;35;310;8;290;5;0;25;6;0;6;5;46;35;40;5;5B0;
25;36;5;6;5;6;20;25;J0;9;4G0;5;30;5;36;0;6;0;5;6;20;5;D0;9;3G0;8;G0;5;40;6;5;36;0;46;0;5;40;5
;570;5;20;25;26;5;46;5;0;6;100;9;60;9;270;8;1J0;5;0;26;20;6;0;6;30;6;20;5;80;9;7;0;9;0;9;1I0;8
;140;8;210;25;6;25;6;30;5;2F0;8;2G0;5;20;6;5;0;25;0;5;0;5;0;6;25;20;5;70;9;50;7;520;5;6;0;5;C0
;9;60;9;240;8;D0;8;110;8;100;5;60;5;E0;9;60;9;230;8;A0;28;250;5;0;5;30;A;40;2A;0;9;20;9;0;9;0
;9;0;29;60;29;40;9;C0;9;3E0;5;40;5;20;5;A;30;2A;70;9;0;7;40;9;80;9;0;9;240;8;D0;8;1A0;5;B;0;B
;B0;9;7;9;40;9;0;9;50;7;9;20;9;110;9;180;8;30;8;1H0;B;0;B;40;B;2A;0;C;A;80;39;0;29;0;9;30;29;
0;29;20;29;2B0;28;C0;8;110;B;0;B;40;B;0;2A;0;2A;90;39;7;0;29;40;29;40;9;200;8;260;B;30;B;0;B;
80;D;B;0;29;0;9;0;9;40;27;9;0;9;20;29;0;9;30;9;40;9;1A0;8;100;8;160;B;30;D;30;B;20;2D;B;30;B;
50;9;0;7;40;7;20;9;0;9;0;9;0;39;4A0;D;30;B;D;2B;D;50;B;30;9;0;7;9;30;7;29;7;9;7;9;20;9;7;20;9;
220;8;E0;8;0;8;20;8;170;2B;0;B;40;B;60;9;60;9;7;9;27;9;0;9;30;49;7;0;9;60;29;1A0;8;260;B;0;B;
0;B;0;B;0;D;20;B;0;2B;30;B;30;7;9;7;9;0;9;7;29;77;9;7;29;0;7;9;7;9;20;9;420;B;30;B;0;D;50;B;60
;7;0;29;27;9;27;0;27;29;0;9;0;9;0;7;20;29;50;9;3H0;B;30;D;0;B;20;B;20;B;70;9;7;0;9;7;9;7;0;37;
9;77;20;9;7;0;9;0;7;410;B;D;50;2B;A0;29;0;29;7;9;57;9;7;9;7;9;0;9;7;9;7;29;7;0;9;B0;9;3F0;B;D;
B;0;B;0;B;40;B;0;D;9;0;7;0;47;9;37;9;77;9;37;9;37;40;9;3D0;B;60;B;0;B;0;2B;40;B;0;B;30;9;30;7;
9;20;7;9;37;9;47;9;27;0;7;0;7;29;7;9;450;B;0;B;20;B;20;B;0;B;30;9;20;7;9;0;H7;0;37;39;60;9;380
;B;40;B;20;D;B;0;2B;0;2D;B;0;3B;30;7;9;27;0;97;9;27;9;97;20;9;400;2B;0;B;D;20;B;90;B;0;9;7;9;1
37;9;7;9;60;9;0;9;390;2B;40;B;0;B;20;B;B0;9;0;9;47;9;77;9;57;9;27;49;7;9;20;9;40;9;40;9;350;B;
0;D;0;4B;40;B;60;39;27;9;7;0;9;37;9;B7;9;7;9;7;0;9;27;3B0;B;40;B;20;B;20;B;20;B;60;B;20;D;B;0;
29;20;47;0;27;9;97;9;27;0;27;29;3G0;3B;0;2B;60;B;30;B;20;B;20;D;0;29;27;0;I7;9;7;9;7;9;37;0;9
;0;39;3C0;B;30;B;30;B;A0;9;0;7;0;9;7;9;77;9;47;9;7;9;7;9;0;7;39;0;7;50;7;20;9;320;B;60;B;0;2B;
D;20;B;90;D;0;2B;137;9;47;0;9;20;9;30;9;390;B;70;2B;90;B;20;39;7;0;27;9;F7;9;37;9;7;9;20;7;49
;350;B;60;B;30;B;A0;B;30;B;D;47;9;27;9;D7;0;47;0;7;0;29;20;9;20;9;0;9;310;B;30;B;30;B;0;B;E0;
2B;0;D;0;9;47;9;F7;0;29;7;9;40;9;60;9;350;B;30;B;40;B;60;B;D;0;B;20;B;D;9;7;9;7;9;77;9;A7;20;
27;9;0;9;20;9;3G0;B;E0;2B;20;D;20;7;9;0;7;9;27;29;27;9;57;9;7;0;7;9;7;9;27;0;7;4G0;B;0;D;47;0;
7;29;77;9;87;0;9;7;20;39;0;9;3F0;D;H0;2B;0;7;9;0;9;7;29;20;7;0;7;9;7;0;9;37;9;37;29;0;9;0;9;40
;9;4D0;B;0;7;9;7;0;7;9;7;9;27;20;9;57;9;27;0;7;0;29;7;0;9;40;9;3G0;B;G0;2B;0;37;9;7;9;7;0;37;0
;9;27;20;9;20;7;420;B;F0;2B;20;B;40;29;7;9;27;9;0;39;7;0;7;9;0;7;20;29;0;7;29;0;9;4F0;B;0;B;20
;D;20;27;9;27;0;9;0;7;30;9;7;9;20;9;0;29;40;9;440;B;F0;B;0;9;0;9;20;39;0;9;7;9;20;29;20;9;70;2
9;440;B;F0;9;40;9;0;29;30;7;20;9;0;7;60;9;570;9;30;7;0;9;20;9;5G0;9;7;20;9;0;29;H0;9;560;E;0;
E;30;F;9;0;2E;20;E;0;29;4J0;E;40;E;60;E;B0;E;20;E;4F0;E;80;E;190;E;540;E;640;E;70;E;20;E;850;
E;4D0;E;CE0;E;1IB0;E;I40;E;50;E;20;E;90;E;G0;E;30;E;20;E;0;E;70;E;B0;

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 REF : M60-SM-000 Rev 02
Decompression

Expanding data is exact reverse process. First, you would extract the LUT from the given data, this LUT is
separated from data by a pipe character ‘|’.
In the previous example, the LUT would be:

0;46;47;51;50;A;B;1F;28;1E;32;14;33;15;3C;3D

..which says: “ok, this is a compression with up to 16 different char values !”.
In 10-base, those values are 0;86; 87; 101;35;48;100;62;24;63;25;72;73 (remember, we’re using 20-base
storing conversion.)

Once you get your real values, you just put into an array of 16KB the result of value conversion. Data are
actually 20-base indexes that refer to the LUT table.
For instance, 61H0 says “I put 2437 times the value indexed by ‘0’ which is 0”.. and so on..

bool UTIL::UnSqueezeMoreMatrix( u_char* pcData , QString &strSrc)

{
bool bOk = false;

//get LUT back !

QList<uchar> lstLUT;

QStringList lststrLUT = strSrc.mid(0, strSrc.indexOf("|")).split(";");

foreach(QString str, lststrLUT)
lstLUT.append(str.toUInt(&bOk, COMP_BASE_CONV));

//split data into a list of items to convert

QStringList lstItem = strSrc.mid(strSrc.indexOf("|") + 1, strSrc.size()).split(";");

uint uiCurrOffset = 0;
foreach(QString strItem, lstItem)
{

if(strItem.size() == 1)
{//if item is 1-byte long, then convert it and put it once in target array
*(pcData + uiCurrOffset) = lstLUT.at(strItem.toUInt(&bOk, COMP_BASE_CONV));
uiCurrOffset++;
}
else
{//if item is longer, put the converted value as many times as needed
uint uiNbOcc = strItem.mid(0, strItem.size() - 1).toUInt(&bOk, COMP_BASE_CONV);
uchar ucVal = lstLUT.at(strItem.mid(strItem.size() - 1, strItem.size() ).toUInt(&bOk,
COMP_BASE_CONV));
memset(pcData + uiCurrOffset, ucVal, uiNbOcc);
uiCurrOffset += uiNbOcc;
}
}

return true;

Page 62/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02
CALIBRATION

M60 Host

M60;1;510207-000451;;BILL[CR]
Sending calibration report
CALIBRATION

Sending calibration report

M60;1;510000-000000;;A;
CALIBRATION
DATE;14/05/2017
TIME;15:31:36
OPERATOR;B
ITEM_CALI;5
COEFF
WBC;1.042
RBC;1.090
HGB;1.046
MCV;1.111
PLT;1.290
MPV;1.133
TARGET
UNIT;1
LOT;PLUS0517
LOT DATE;27/04/2017
LOT TIME;14:13:22
EXPIRY DATE;05/06/2017
OPERATOR;BIOLO
SOURCE;O
WBC;9.0 ;0.0
RBC;4.62 ;0.0
HGB;13.1;0.0
MCV;84.9 ;0.0
PLT;283 ;0.0
MPV;;0.0
END_CALI;11528

M60;W;X;;Y;[CR]

Page 63/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Where :
W is the Free instrument number (2 chars maximum).
X is the Free serial number (13 chars).
Y is the user login, UTF8 coded.

Following by the N results used for the calibration.

Result

M60;1;510000-000000;;A;
RESULT
DATE;27/04/2017
TIME;14:40:37
MODE;CALIBRATION
UNIT;1
SEQ;1;0
TEST;LMG
OPERATOR;LL
PREL;OT
CYCLE;N
WBC;8.7 ; ;L; ;
RBC;4.31 ; ;L; ;
HGB;12.4; ;L; ;
MCV;76.5 ; ;L; ;
PLT;220 ; ;L; ;
MPV;;;;;
END_RESULT;1302

Page 64/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02
QC

M60 Host

M60;1;510207-000451;BILL[CR]
Sending QC result
QC

Result

M60;1;510000-000000;;A;
RESULT
DATE;27/04/2017
TIME;15:05:01
MODE;QC
UNIT;1
SEQ;26
LOT;MYT1704L
LOT DATE;27/04/2017
LOT TIME;14:58:45
LEVEL;L
EXPIRY DATE;05/06/2017
USER;BIOLO
OPERATOR;BIOLO
PREL;OT
CYCLE;N
WBC;2.6 ; ; ; ;2.2 ;3.2 ;
LYMP;49.9; ; ; ;43.8;59.8;
MONP;13.5; ; ; ;5.5 ;19.5;
NEUP;33.1; ; ; ;24.2;40.2;
LYM;1.3 ; ; ; ;1.1 ;1.7 ;
MON;0.4 ; ; ; ;0.1 ;0.5 ;
NEU;0.9 ; ; ; ;0.6 ;1.2 ;
RBC;2.25 ; ; ; ;2.02 ;2.32 ;
HGB;6.0 ; ; ; ;5.4 ;6.4 ;
HCT;17.7; ; ; ;14.7;18.7;
MCV;78.6 ; ; ; ;71.8 ;81.8 ;
MCH;26.7; ; ; ;24.5;29.5;
MCHC;33.9; ; ; ;32.1;38.1;
RDW-CV;10.6; ; ; ;8.4 ;16.4;

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 REF : M60-SM-000 Rev 02
RDW-SD;34.8 ; ; ; ;33.7 ;41.7 ;
PLT;46 ; ; ; ;28 ;68 ;
MPV;11.4; ; ; ;8.6 ;14.6;
END_RESULT;22174

Repeatability
No emissions.

Control sum
The control sum is a CRC code : CRC-16.

Algorithmic
The used source code is available here below( C code ).
It allows generating standard CRC-16.

This algorithm is mainly used in embedded systems ( like hard disk controller).
For best performance, compute is done from a seek table with 16 values.
Here is the CRC computation:

CRC = 0xFFFF
For each nibble :
Work on high weight nibble :
Index = byte EXCLUSIVE OR CRC
Index = Index AND 000F
CRC = Table(Index) EXCLUSIVE OR (CRC divided by 16)
Work on low weight nibble :
Index = byte divided by 16
Index = Index EXCLUSIVE OR CRC
Index = Index AND 000F
CRC = Table(Index) EXCLUSIVE OR (CRC divided by 16)

C language source example implementation

Seek table declaration :

static const unsigned short ausCrcTab1[] =
{
0x0000, 0xCC01,0xD801,0x1400,0xF001,0x3C00,0x2800,0xE401,
0xA001,0x6C00,0x7800,0xB401,0x5000,0x9C01,0x8801,0x4400,
};

CRC computation :

unsigned short calc_crc(unsigned char *pucData, long lSize)

{
unsigned short usAcc1 = 0xFFFF;

while ( lSize > 0 )

{
/* gestion par quartet du calcul */
usAcc1 = ausCrcTab1[(*pucData ^ usAcc1) & 15] ^ (usAcc1 >> 4);
usAcc1 = ausCrcTab1[((*pucData >> 4) ^ usAcc1) & 15] ^ (usAcc1 >> 4);

Page 66/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

pucData++;
lSize--;
}

return(usAcc1);
}

^ : EXCLUSIVE OR
>> : logical right shift.
& : logical and.

This CRC is computed form the beginning sent data until the end of line ([CR] included ) preceding the checksum
line ( identifier + value ).

Page 67/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

6. PARTS LIST

6.1 Spare parts list

Part name Part number

Rocker Guide CT/AL/M60 1002180117-SHL
M60 - Counting Holder 1004180001-SHL
M60 - Syringe Manifold 1004180002-SHL
M60 - Manifold 2 Valves 1004180003-SHL
M60 - Heating Assy. 1004180004-SHL
M60 - Optical Bench Assy. 1004180005-SHL
M60 - Flowcell Assy. 1004180006-SHL
M60 - Vacuum Assy. 1004180007-SHL
M60 - Body Screw Valve 1004180008-SHL
M60 - CPU Board (without processor) 1004180009-SHL
M60 - Processor board 1004180010-SHL
M60 - I/O Board 1004180011-SHL
M60 - HGB Board 1004180012-SHL
M60 - On/Off Board 1004180013-SHL
M60 - USB Board 1004180014-SHL
M60 - Front Cover 1004180015-SHL
M60 - Fluidic Door 1004180016-SHL
M60 - Reagent Door 1004180017-SHL
M60 - Rear Plate 1004180018-SHL
M60 - Upper Plate 1004180019-SHL
M60 - Sampling Needle 1004180020-SHL
M60 - Shifting Slab Assy. 1004180021-SHL
M60 - In Line Pressure Sensor 1004180022-SHL
M60 - Diluent Temperature Sensor 1004180023-SHL
M60 - RBC Counting Head 1004180024-SHL
M60 - WBC Counting Head 1004180025-SHL
M60 - RBC Counting Bath 1004180026-SHL
M60 - WBC Counting Bath 1004180027-SHL
M60 - WBC Electrode Module 1004180028-SHL
M60 - RBC Electrode Module 1004180029-SHL
M60 - Tubings Kit 1004180030-SHL
M60 - PU Tubing 1.6 x 3.2 mm 1004180031-SHL
M60 - PU Tubing 2.4 x 4 mm 1004180032-SHL
M60 - PU Tubing 3.2 x 6.4 mm 1004180033-SHL
M60 - PU Tubing 3.2 x 4.8 mm 1004180034-SHL
M60 - Tubing #33 1004180035-SHL
M60 - Waste Tubing 1004180036-SHL
M60 - Diluent Pick Up Tubing 1004180037-SHL
M60 - Connector Straight Reducer 1.6/2.4 mm 1004180038-SHL
M60 - Connector Straight Reducer 2.4/3.2mm 1004180039-SHL
M60 - Connector Straight 3,2 mm Nylon 1004180040-SHL
M60 - Connector Y 3.2 mm 1004180041-SHL

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 REF : M60-SM-000 Rev 02
M60 - Connector Y 2.4 mm 1004180042-SHL
M60 - Connector Y 1.6 mm 1004180043-SHL
AL - M60 Waste Fitting Assy. 1004180044-SHL
AL - M60 Diluent Fitting Assy. 1004180045-SHL
M60 - Stopcock Lyse 1004180046-SHL
M60 - Stopcock Luer Connector 1004180047-SHL
M60 - Start Key Switch 1004180048-SHL
M60 - Start Key 1004180049-SHL
M60 - Fluidic Door Switch 1004180050-SHL
M60 - Electrovalve 2/2 1.6mm 1004180051-SHL
M60 - Electrovalve 3/2 1.6mm 1004180052-SHL
AL - M60 LCD Data Cable 1004180055-SHL
M60 - Pump Vibr. Grommet 1004180056-SHL
M60 - Fitting Barbed Ø1.2 Red 1004180057-SHL
M60 - Fitting Barbed Ø1.2 Black 1004180058-SHL
M60 - Fitting Barbed Ø1.2 Green 1004180059-SHL
M60 - Fitting Barbed Ø1.6 Natural 1004180060-SHL
M60 - Fitting Barbed Ø1.6 Blue 1004180061-SHL
M60 - Fitting Dummy Yellow 1004180062-SHL
M60 - Stainless Fitting 1004180063-SHL
M60 - Chamber Protection 1004180064-SHL
M60 - O-ring Ø 14.3 x 2.4 Silicone 1004180065-SHL
M60 - O-ring Ø 2.5 x 1.25 Nitrile 1004180066-SHL
M60 - Motors Cable 1004180067-SHL
M60 - Electrovalves Cable (x14) 1004180068-SHL
M60 - Electrovalves Ext. Cable (x4) 1004180069-SHL
M60 - Power Supply Cable 1004180070-SHL
M60 - CPU Rear Panel Cable 1004180071-SHL
M60 - FSC Cable 1004180072-SHL
M60 - ALL Cable 1004180073-SHL
M60 - RBC Cable 1004180074-SHL
M60 - WBC Cable 1004180075-SHL
M60 - Counting Cable 1004180076-SHL
M60 - Heater Pump Cable 1004180077-SHL
M60 - LED Cable 1004180078-SHL
M60 - USB Cable 1004180079-SHL
M60 - ON/OFF Cable 1004180080-SHL
M60 - USB Ground Cable 1004180081-SHL
M60 - Counting Ground Cable 1004180082-SHL
M60 - Syringe Shielding Wire 1004180083-SHL
M60 - Syringe Body 1004180084-SHL
M60 - Motor Holder 1004180085-SHL
M60 - Syringe Motor 1004180086-SHL
M60 - USB Barcode Scanner 1004180087-SHL
M60 - Optical Led Board 1004180088-SHL

Page 69/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02
6.2 Two/Three years spare parts kit

Every 1100 days or 90’000 samples

Part name Part number

M60 - Syringe Body 1004180084-SHL
Piston Ø 22 x2 005-1001-03 03
Piston Ø 16 - Syringes module 010-2501-03 02
Piston Ø 6.5 - Syringes module 010-2501-03 01
Syringe Piston Ø 1.6 005-1001-03 09

Page 70/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

7. SOFTWARE
7.1 Introduction
Please refer to the User’s Manuel for explanation of the software operation.

7.2 Specific technician display

The following section describe the software operation which are only accessible with the technician and super
technician passwords.

7.2.1 Technician password

The technician password is :

GT9XB12
NOTE: in order to log-in you need to put your initials in user name field

- It is strictly forbidden to disclose the technician password to the final customer.

- In such case, Orphée guarantee will not be valid.

- In the technician log mode the most part of the control of the MYTHIC 60 are
invalided, perform a specific Orphée training before repairing or maintaining.

Page 71/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

7.2.2 Technician display

The technician display are accessible from MAIN SCREEN then SERVICE then TECHNICIAN.

7.2.2.1 Technician

Allows access to the adjustment of the optical parameters

Allows access to the adjustment of the impedance and HGB

parameters

Allows access to the menu where operator can control optical gain
parameters by using fresh blood sample

In this menu operator can control QC dif threshold

In this menu operator can change analyzer SN and enable Altitude

option

In this menu operator can reset and adjust maintenance counter

settings

7.2.2.2 Adjust dif

- The modification of one of these parameters must be done in compliance with

Orphée after having performed a specific training.
- In this case of modification without authorization, Orphée guarantee will not be
valid.

Allows to adjust LED electric gain

Allows to run transverse adjustment (WBC latex),

Check button displays latex cloud position

Allows to adjust optical axis gains (G-CAL),

Check button displays G-CAL cloud position

NOTE: to use Adj. or Check button function testing specimen need to be given for examination
from sampling needle separately

Page 72/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02
7.2.2.3 Adjust other

- The modification of one of these parameters must be done in compliance with Orphée
after having performed a specific training.
- In this case of modification without authorization, Orphée guarantee will not be
valid.

Latex WBC
Allows to adjust impedance gain for WBC
channel
Check button displays latex curve position on
a histogram

Latex RBC
Allows to adjust impedance gain for RBC
channel
Check button displays latex curve position on
a histogram

Allows to adjust electronic gain for HGB

channel

Allows to adjust pressure sensor reading for

current instrument altitude

NOTE: to use Adj. or Check button function testing specimen need to be given for examination
from sampling needle separately

Page 73/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02
7.2.2.4 Check dif WB

- The modification of one of these parameters must be done in compliance with

Orphée after having performed a specific training.

Allows to adjust LED electric gain

Fresh blood sample

Allows to check correctness of G-CAL
calibration.

7.2.2.5 QC dif tresholds

- The modification of one of these parameters must be done in compliance with Orphée
after having performed a specific training.
- In this case of modification without authorization, Orphée guarantee will not be
valid.

This menu allows technician to

enable fix dif thresholds for
QC analysis. By pressing on
ENABLE QC FIX
TRESHOLDS position of each
discriminator on a
scattergram can be adjusted.

Page 74/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

7.2.2.6 Maintenance

- The modification of one of these parameters must be done in compliance with

Orphée after having performed a specific training.

This menu allows an operator to reset and adjust maintenance counter. Analyzers requires minimum one flush
cleaning per week. There is also a need to perform short and long maintenance on preset periods. The time
and number of samples can be changed within acceptable ranges.

Flush cleaning should be done minimum once per week for a workload
< 50 samples per day. Higher number of samples requires greater
number of cleaning.
Cycles range: 100-300, Days range 2-7

Short maintenance includes: rinsing head O-ring

replacement and pistons greasing.
Cycles range: 9000-11000, Days range 150-210

Big maintenance includes: rinsing head O-ring, pistons,

syringe body replacement. Pistons, rocker and endless
screw mechanisms should be greased.
Cycles range: 80000-100000,
Days range 1000-1200

7.2.2.7 System config

- The modification of one of these parameters must be done in compliance with Orphée
after having performed a specific training.
- In this case of modification without authorization, Orphée guarantee will not be
valid.

In this menu technician can change analyzer

serial number and enable altitude option

Page 75/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02
7.2.3 Troubleshooting
This menu allows to check all functionalities of analyzer.
7.2.3.1 Menu overview

Control cycle function

Allows to run advance cleaning cycle,

Use Mythic 18-22 FLUSH-CLENER

This function removes liquid from counting baths

This function backflushing apertures

This function backflushing optical flow cell

This function moves needle in a position where is easy to

remove from its holder

This function checking rocker movement

This function motors motors in home positions

This function rinsing counting baths

This function runs autocleaning, it will clean fluidic system by

using cleaner and diluent

Automatic cleaning and draining function for storage

Its moves syringes to greasing positions

This function removes liquid from flow cell

Additional menu where user can check sensor and valves

functions

This function checking syringe functions

This function checking needle functions

Page 76/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02
7.2.3.2 Check sensors / valves

Those buttons checks

valves functions

SET OPT LED ON

checking optical LED
SET AC ON
turns on aperture
current
SET HGB LED OFF
checking HGB LED
TEST VACUUM
syringe vacuum test
TEST PUMP
Pump vacuum test

Information about Realtime reading Realtime reading

motors home from optical LED from pressure
positions and keys power (ALL) and HGB sensors and temp
status power reading

Page 77/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02
7.2 Software Tree
ACCESS
MENU SUB-MENU FUNCTION OPERATOR ADMINISTRATOR TECHNICIAN

STARTUP
SHUTDOWN
CHANGE LOAD
QC LJ GRAPHS
RESULT
DETAILS
CHANGE
STATS
CALIBRATION RESULT Calibrate
LOAD
DEL.UNSELECT
REPEATABILITY
DETAILS
NEXT SAMPLE
RUN SAMPLE
DIF WB
TABLE VIEW
DATE
RESULTS DETAILS
DETAILS
PRIME ALL
DILUENT PRIME
LYSE PRIME
REAGENTS CLEANER PRIME
WASTE RESET
Cycle Counters RESET
Reagents Log
SYSTEM INIT
EVENT LOGS
ERROR LOGS
BACKUP
BACKUP & RESTORE
RESTORE
LAB
PREFERENCES
UNITS
CBC
TRHESHOLDS
LAB
AND FLAGS
PARAMETERS
DIFF FLAGS
REFERENCE
RANGES
CALIBRATION
FACTORS
DATE AND TIME
AUTOMATIC
SETTINGS
CYCLES
SERVICE
PRINTER
SETTINGS
PRINTER
PRINTERS
MANAGEMENT
COMMUNICA- COMMUNICA-
TION TION SETUP
ADD
USERS CHANGE
MANAGEMENT PASSWORD
REMOVE
SOFTWARE
UPDATE
FLUIDIC
CONTROL
FLUSH
TROUBLESHOOTING CLEANING
DRAIN BATHS
BACKFLUSH
APERTURES

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 REF : M60-SM-000 Rev 02
BACKFLUSH OPT.
BENCH
NEEDLE
DISMANTLING
CHECK ROCKER
PARK MOTORS
RINSE
CLEAN
DRAIN FOR PACK
UP
SYRINGE
GREASING
DRAIN FLOW
CELL
EV1-EV18
ALL EV ON
EV CHASER
SET HGB LED
CHECK
OFF
SENSORS/
SET AC ON
VALVES
SET OPT LED
ON
TEST VACUUM
TEST PUMP
CHECK SYRINGE
CHECK NEEDLE
ADJ. LED
ADJ. OB
ADJUST DIF CHECK OB
ADJ.WBC
CHECK WBC
WBC ADJUST
WBC CHECK
RBC ADJUST
ADJUST
RBC CHECK
TECHNICIAN OTHERS
HGB ADJUST
PRESSURE
ADJUST
CHECK DIF WB ADJ. LED
CHECK DIF
QC DIF FACTORY
THRESHOLDS SETTINGS
MAINTENACE RESET
SYSTEM CONFIG
LOG OUT

Page 79/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

8. MAINTENANCE
The quality of the results and the reliability of the MYTHIC 60 are directly linked to the strict respect of
the maintenance hereafter described.

To perform the maintenance and the repair described in this section, it is mandatory to wear
rubber gloves and wash hands with a disinfectant after completion of work.

8.1 Maintenance table

NOTE: This maintenance table is dedicated to the user and to service maintenance people. Mythic
60 will give information on the screen when Flush cleaning, short or Big maintenance are needed.
Regarding the pistons greasing, it can be made as often as possible but not longer than 6 months
otherwise the lifetime of the hydraulic pistons and the performances of the instrument could be
reduced.

Startup Flush Pistons Short Big

Shutdown cleaning greasing maintenance maintenance

Daily

Every 5 days or 200 

samples

Every 180 days or 

10’000 samples  
Every 1100 days or
90’000 samples 

Action performed by the user

Action performed by service technician

Page 80/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02
8.2 Flush Cleaning

FLUSH CLEANER allows performing a concentrate cleaning cycle using HM18-011-25 FLUSH-CLEANER

1. Press on button, the following window is displayed.

 Select to confirm, the following window is displayed.

2. Instrument begins to drain the counting chambers and the following window is displayed.

3. Open the fluidic door and put 4ml of Flush-Cleaner in each counting chamber. Close the fluidic
door when bleach dispense is done, then press . The system will perform fluidic system
cleaning the mains counting module parts like apertures, counting chambers and optic flow cell.

NOTE: On each counting bath, an arrow indicates the volume of 4ml for bleach. The bleach
cycle takes 15 minutes to be completed. It is not possible to use the instrument during that
time.

Page 81/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02
8.3 Syringes Greasing
SYRINGES GREASING option allows moving the pistons down in order to perform the greasing.

NOTE: The piston greasing must be performed minimum every 6 months, please proceed as describe in
the procedure below.

1. Press on and wait for the following information window to appear.

2. Wear rubber glove on one hand and place a bit of silicon grease at the tip of the index finger.

3. Spread a thin film of grease on each piston excepted sampling piston.

NO GREASE
ON SAMPLING
PISTON

NOTE: With a tool key type T20, turn the two bigger pistons (waste pistons) in order to
spread the grease all around the pistons.

Page 82/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

9. REPAIRING
For all the following operations wear rubber gloves and wash hands with a disinfectant after
completion of work.
Be careful with the edge of the metal sheets after having dismantled of the covers.

Emergency Stop
In case of mechanical or hydraulic problem, immediately press STOP button, the MYTHIC 60 will make a
emergency stop. After having identified the problem, it is necessary to perform a SYSTEM INIT

Syringe Valves Module #1

Syringe valves (from syringe Valves Module #1) replacement

Tools/Material • Torx tool T06

Removal
Action Steps Reference
1. Drain the instrument from any liquids. 1. Remove pick up tubings from
Prerequisite their containers and select
REAGENT/ PRIME ALL.
2. Switch off the instrument and disconnect 2. Refer to the instruction.
the main power cord.
3. Remove the fluidic door. 3. Refer to the instruction.
4. Remove the reagent door. 4. Refer to the instruction.
5. Remove the top cover. 5. Refer to the instruction.
6. Remove the reagent plate. 6. Refer to the instruction.

Syringe NOTE: Syringe valve module #1 is composed

Valves with 2 valves 3/2-1.6mm.
Disassembling
1. Disconnect the valve #18 connector.

V18 V12

NOTE: As an example, the following instruction

will describe the way how to replace the valve
#18, proceed as the same in case of change of
valve N°12.

2. Remove the two fixation screws of the

valve solenoid.

Page 83/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

3. Remove the solenoid from the valve body.

4. Remove the two fixation screws of the

valve body.

5. Remove the valve body from the support.

6. Install the new valve following the

reverse instruction.

NOTE: Be careful to the mounting direction of the

valve body.
The inscription must be oriented through the
right for the two valves which compose the
module.

1. Tighten the two fixation screws of the

valve body, respecting a torque to 40cNm.

40cN

2. Install the valve solenoid on the valve body.

NOTE: Be careful to the mounting direction of

the valve solenoid. Connector must be oriented
through the top.
25cN
3. Tighten the two fixation screws of the
valve solenoid, respecting a torque to
25cNm.

Page 84/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Syringe Valves module #1 replacement.

Tools/Material • Torx tool size T20

DISMANTLING
Action Step Reference

Prerequisite 1. Drain the instrument from any liquids. 1. Remove pick up tubings from their
containers and select REAGENT/
PRIME ALL.
2. Switch off the instrument and disconnect 2. Refer to the instruction.
the power supply. 3. Refer to the instruction.
3. Remove the fluidic door. 4. Refer to the instruction.
4. Remove the reagent door. 5. Refer to the instruction.
5. Remove the top cover. 6. Refer to the instruction.
6. Remove the reagent plate

Dismantling 1. Disconnect the six tubings from the

assembly.

Dismantling 2. Disconnect the valves connectors.

V18 V12

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 REF : M60-SM-000 Rev 02

Dismantling 3. Remove the two fixation screws.

4. Remove the valves assembly from the unit.

V18 V12

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 REF : M60-SM-000 Rev 02

.
Syringe Valves Module #1 Assembling

Tools/Material • Torx tool size T20

ASSEMBLING
Action Step Reference

Prerequisite 1. Follow the instruction of Syringe 1. Refer to the instruction.

Valves module #1 dismantling.
.

1. Install the valve assembly.

Assembling 2. Secure the assembly with the two
fixation screws tighten to the dedicated
torque.
100 cNm

V18 V12

Assembling 3. Connect the six tubings to the assembly.

Completion 1. Install the reagent plate. 1. Refer to the instruction.

2. Install the top cover.
3. Install the fluidic door. 2. Refer to the instruction.
4. Install the reagent door. 3. Refer to the instruction.
5. Connect the power supply and Switch on 4. Refer to the instruction.
the instrument. 5. Refer to the instruction.
6. Perform a prime all cycle. 6. Refer to the instruction.

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 REF : M60-SM-000 Rev 02

Syringe Valves Module #2

Syringe valves (from syringe Valves Module #2) replacement

Tools/Material • Torx tool T06

Removal
Action Steps Reference

Prerequisite 1. Drain the instrument from any liquids. 1. Remove pick up tubings from
their containers and select
REAGENT/ PRIME ALL.
2. Switch off the instrument and disconnect 2. Refer to the instruction.
the main power cord.
3. Remove the fluidic door. 3. Refer to the instruction.
4. Remove the reagent door. 4. Refer to the instruction.
5. Remove the top cover. 5. Refer to the instruction.
6. Remove the reagent plate. 6. Refer to the instruction.

Syringe NOTE: Syringe valve module #2 is composed

Valves with 2 valves 3/2-1.6mm
Disassembling
1. Disconnect the valve #6 connector.
V6 V5

NOTE: As an example, the following instruction

will describe the way how to replace the valve
#6, proceed as the same in case of change of
valve N°5.

2. Remove the two fixation screws of the

valve solenoid.

3. Remove the solenoid from the valve body.

4. Remove the two fixation screws of the

valve body.

5. Remove the valve body from the support.

6. Install the new valve following the

reverse instruction.

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 REF : M60-SM-000 Rev 02

NOTE: Be careful to the mounting direction of

the valve body.
The inscription must be oriented through the
right for the two valves which compose the
module.

1. Tighten the two fixation screws of the

valve body, respecting a torque to 40cNm.

40cN

2. Install the valve solenoid on the valve body.

NOTE: Be careful to the mounting direction of

the valve solenoid. Connector must be oriented
25cN
through the down.

3. Tighten the two fixation screws of the

valve solenoid, respecting a torque to
25cNm.

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 REF : M60-SM-000 Rev 02

Syringe Valves module #2 dismantling

Tools/Material • Torx tool size T20

DISMANTLING
Action Step Reference

Prerequisite 1. Drain the instrument from any liquids. 1. Remove pick up tubings from their
containers and select REAGENT/
PRIME ALL .
2. Switch off the instrument and disconnect 2. Refer to the instruction.
the power supply.
3. Remove the fluidic door. 3. Refer to the instruction.
4. Remove the reagent door. 4. Refer to the instruction.
5. Remove the top cover. 5. Refer to the instruction.
6. Remove the reagent plate 6. Refer to the instruction.

Dismantling 1. Disconnect the six tubings from the

assembly.

Dismantling 2. Disconnect the valves connectors.

V6 V5

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Dismantling 3. Remove the two fixation screws.

4. Remove the valves assembly from the unit.

V18 V12

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 REF : M60-SM-000 Rev 02
Syringe Valves module #2 assembling

Tools/Material • Torx tool size T20

ASSEMBLING
Action Step Reference

Prerequisite 1. Follow the instruction of Syringe 1. Refer to the instruction.

Valves module #2.

1. Install the valve assembly.

Assembling
2. Secure the assembly with the two
fixation screws tighten to the dedicated
torque. 100 cNm

V6 V5

Assembling 3. Connect the six tubings to the assembly.

Completion 1. Install the reagent plate. 1. Refer to the instruction.

2. Install the top cover. 2. Refer to the instruction.
3. Install the fluidic door. 3. Refer to the instruction.
4. Install the reagent door. 4. Refer to the instruction.
5. Connect the power supply and Switch on 5. Refer to the instruction.
the instrument.
6. Perform a prime all cycle. 6. Refer to the instruction.

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 REF : M60-SM-000 Rev 02

Counting Module (RBC/WBC Chambers, Heads, Electrodes, Counting Holder)

RBC Electrode.

Tools/Material • Torx tool T10

Removal
Action Steps Reference

Prerequisite 1. SERVICE/TROUBLESHOOTING/DRAIN
BATHS 2. Refer to the instruction
2. Switch off the instrument and disconnect
the main power cord. 3. Refer to the instruction
3. Open the fluidic door.

Electrode 1. Turn the electrode plastic holder from a

RBC quarter turn anticlockwise.
2. Pull on it and remove the RBC electrode
assembly from the RBC counting
chamber.

1
3. Remove the ground cable fixation screw (1)
of the RBC electrode.

4. Disconnect the RBC coaxial connector (2) from

the counting manifold and then remove the RBC
electrode from the instrument. 2

5. Replace the RBC electrode respecting

the reverse instruction.

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 REF : M60-SM-000 Rev 02
RBC Counting Chamber replacement

Tools/Material • Torx tool T10

Removal
Action Steps Reference

Prerequisite 1. SERVICE/TROUBLESHOOTING/DRAIN
BATHS
2. Switch off the instrument and disconnect
the main power cord.
3. Open the fluidic door.
4. Remove the RBC electrode.

RBC 1. Release the maintaining screw (1) of

Chamber the chambers protection and remove it.

2. Remove the three remaining fixation screws (2)

of the RBC chamber.
2 2

3. Pull on the RBC chamber and remove it.

4. Replace the RBC chamber following the

reverse instruction.

Note: Verify the presence of the O-ring on

the red fitting before to perform the
reassembling.
38

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 REF : M60-SM-000 Rev 02

Note: Verify the presence of the three O-

rings on the chamber before to perform the
reassembling.

38 38

38

Important note: Screw back the

four fixation screws respecting a
50cNm
torque to 50cNm

5. Reinstall the chambers protection.

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 REF : M60-SM-000 Rev 02

RBC Counting Head replacement

Tools/Material • Torx tool T08

• 4K05 Microparticles

Removal
Action Steps Reference

Prerequisite 1. SERVICE/TROUBLESHOOTING/DRAIN
BATHS 2. Refer to the instruction
2. Switch off the instrument and disconnect
the main power cord. 3. Refer to the instruction
3. Open the fluidic door. 4. Refer to the instruction
4. Remove the RBC electrode. 5. Refer to the instruction
5. Remove the RBC counting chamber.

RBC 4. Remove the two fixations screws (1) of the RBC

Counting counting head.
Head

NOTE: RBC counting head is identified with a

red dot.

5. Remove the RBC counting head using pliers to

pull it if necessary.

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6. Replace the RBC counting head following the

reverse instruction.

Note: Verify the presence of the O-ring

before to perform to the reassembling.

30 cNm

Important note: Screw back the two

fixation screws, respecting a torque
to 30cNm.

7. From the same screen, using Latex particles

5µm, perform RBC gain adjustment.

NOTE: Compare the size of the latex

particles with the value in the field
DIAMETER and modify as necessary.

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 REF : M60-SM-000 Rev 02
WBC Electrode replacement

Tools/Material • Torx tool T10

Removal
Action Steps Reference

Prerequisite 1. SERVICE/TROUBLESHOOTING/DRAIN
BATHS 2. Refer to the instruction
2. Switch off the instrument and disconnect
the main power cord. 3. Refer to the instruction
3. Open the fluidic door.

Electrode 1. Turn the electrode plastic holder from a

WBC quarter turn anticlockwise.
2. Pull on it and remove the WBC electrode
assembly from the WBC counting chamber.

3. Remove the ground cable fixation screw (1)

of the WBC electrode. 1

4. Disconnect the WBC coaxial connector (2)

from the counting manifold and then remove
2
the RBC electrode from the instrument.

5. Replace the WBC electrode respecting

the reverse instruction.

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 REF : M60-SM-000 Rev 02
WBC Counting Chamber replacement

Tools/Material • Torx tool T10

Removal
Action Steps Reference

Prerequisite 1. SERVICE/TROUBLESHOOTING/DRAIN 1. Refer to the instruction.

BATHS 2. Refer to the instruction.
2. Switch off the instrument and disconnect 3. Refer to the instruction.
the main power cord.
4. Refer to the instruction.
1. Open the fluidic door.
5. Refer to the instruction.
2. Remove the WBC electrode.
3. Remove the chambers protection.
4. Do HGB GAIN Adjustment

WBC 1. Disconnect the HGB cable connector (1).

Chamber

2. Remove the three remaining screws (2) of the

WBC chamber. 2 2

3. Pull on the WBC chamber and remove it.

4. Replace the WBC chamber following the

reverse instruction.

Note: Verify the presence of the O-ring on

the red fitting before to perform the
reassembling.

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 REF : M60-SM-000 Rev 02

Note: Verify the presence of the three O-

rings on the chamber before to perform the
reassembling.

4 4

Important note: Screw back the

four fixation screws respecting a
torque to 50cNm

50cNm

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 REF : M60-SM-000 Rev 02
WBC Counting Head replacement

Tools/Material • Torx tool T10

• 4K07 Microparticles

Removal
Action Steps Reference

Prerequisite 1. SERVICE/TROUBLESHOOTING/DRAIN 1. Refer to the instruction.

BATHS 2. Refer to the instruction.
2. Switch off the instrument and disconnect 3. Refer to the instruction.
the main power cord.
4. Refer to the instruction.
3. Open the fluidic door.
5. Refer to the instruction.
4. Remove the WBC electrode.
5. Remove the WBC counting chamber. 6. Refer to the instruction
6. perform WBC GAIN Adjustment

WBC 1. Remove the two fixations screws (1) of the WBC

Counting counting head.
Head

2. Remove the WBC counting head using pliers to

pull it if necessary.

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3. Replace the WBC counting head following the

reverse instruction.

Note: Verify the presence of the O-ring

before to perform to the reassembling.

38

Important note: Screw back the two 2

fixation screws, respecting a torque
to 30 cNm.

30 cNm

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 REF : M60-SM-000 Rev 02

4. From the same screen, using Latex particles

7µm, perform WBC gain
adjustment pressing on ADJUST.

NOTE: Compare the size of the latex

particles with the value in the field
DIAMETER and modify as necessary.

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 REF : M60-SM-000 Rev 02
Counting Holder

Tools/Material • Torx tool T10

Removal
Action Steps Reference

Prerequisite 1. SERVICE/TROUBLESHOOTING/DRAIN
BATHS
2. Switch off the instrument and disconnect
the main power cord.
3. Open the fluidic door.
4. Remove the complete equipped RBC chamber.
5. Remove the complete equipped WBC counting
chamber.

Counting 1. Remove the two nuts (1) which maintain the

Holder RBC and WBC coaxial connectors on the
counting holder. 1

2. Once the two nuts removed, push on the coaxial

connectors to remove them from the counting
holder.

3. Remove the ground terminal fixation screw (2).

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4. Disconnect all tubings from the counting holder.

5. Remove the three fixation screws (3) of the

counting holder then, remove it. 3 3

6. Equip the new counting manifold with all

elements and reassemble it following the
reverse instruction.

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 REF : M60-SM-000 Rev 02

Counting Valves Module Disassembly

Counting valves replacement.

Tools/Material • Torx tool 06

Removal
Action Steps Reference

Prerequisite 1. Drain the instrument from any liquids. 1. Remove pick up tubings from
their containers and select
REAGENT/ PRIME ALL.
2. Switch off the instrument and disconnect 2. Refer to the instruction.
the main power cord.
3. Remove the fluidic door. 3. Refer to the instruction.
4. Remove the reagent door. 4. Refer to the instruction.
5. Remove the top cover. 5. Refer to the instruction.
6. Remove the reagent plate. 6. Refer to the instruction.

Counting NOTE: Counting valve module is composed with

Valves 2 valves 2/2-1.6mm-M2.5 .
Disassembling
1. Disconnect the valve connector.

NOTE: As an example, the following instruction

will describe the way how to replace the counting
valve N°1, proceed as the same in case of change
of valve N°2.

1
2. Remove the two fixation screws (1) of the
1
valve solenoid.

3. Remove the solenoid from the valve body.

2
4. Remove the two fixation screws (2) of the
2
valve body.

5. Remove the valve body from the manifold.

6. Install the new valve following the

reverse instruction.

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NOTE: Be careful to the mounting direction of

the valve body.
The “NC” inscription must be oriented through the
bottom for the two valves.

1. Tighten the two fixation screws of the

valve body, respecting a torque to 40cNm.

40cN

2. Install the valve solenoid on the valve body.

NOTE: Be careful to the mounting direction of

the valve solenoid. Connector must be oriented
through the right.

3. Tighten the two fixation screws of the

valve solenoid, respecting a torque to
25cNm.
25cN

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 REF : M60-SM-000 Rev 02

Counting Valves Module Replacement

Tools/Material • Torx tool T20

Removal
Action Steps Reference

Prerequisite 1. Drain the instrument from any liquids. 1. Remove pick up tubings from
their containers and select
REAGENT/ PRIME ALL.
2. Switch off the instrument and 2. Refer to the instruction.
disconnect the main power cord.
3. Remove the fluidic door. 3. Refer to the instruction.
4. Remove the reagent door. 4. Refer to the instruction.
5. Remove the top cover. 5. Refer to the instruction.
6. Remove the reagent plate. 6. Refer to the instruction.

Counting 1. Disconnect the 4 tubings from valves

Valves #1 and #2.
Module

2. Disconnect the two valves connectors.

3. Remove the two fixation screws (1) of

the counting valves module and remove 1
it.

4. Reassemble the counting valves module

following the reverse instruction.

1
NOTE: Torque for fixation screws of
counting valve module is 100 cNm.

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 REF : M60-SM-000 Rev 02

Heater Module Disassembly

Heater replacement.

Tools/Material • Torx tool size T10

DISMANTLING
Action Step Reference

Prerequisite 1. Drain the instrument from any liquids. 1. Remove pick up tubings from
containers and perform prime
2. Switch off the instrument and disconnect all.
the power supply. 2. Refer to the instruction.
3. Remove the fluidic door.
4. Remove the reagent door. 3. Refer to the instruction.
5. Remove the top cover. 4. Refer to the instruction.
6. Remove the reagent plate 5. Refer to the instruction.
6. Refer to the instruction.

Dismantling 1. Disconnect the tubings from the heater.

Dismantling 2. Disconnect the heater connector.

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 REF : M60-SM-000 Rev 02

Dismantling 3. Remove the three (3) fixation screws

located on fluidic side, then remove the
heating system from the unit.

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 REF : M60-SM-000 Rev 02

Heater assembling

Tools/Material • Torx tool size T10

ASSEMBLING
Action Step Reference

Prerequisite 1. Follow the instruction of Heater 1. Refer to the instruction.

dismantling.

Assembling 4. Install the three (3) fixation screws

located on fluidic side.

100 cNm

Assembling 5. Connect the heater connector.

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 REF : M60-SM-000 Rev 02

Assembling 6. Connect the tubings on the heater.

4A

22
11
7

Completion 1. Install the reagent plate. 1. Refer to the instruction.

2. Install the top cover. 2. Refer to the instruction.
3. Install the reagent door. 3. Refer to the instruction.
4. Install the fluidic door. 4. Refer to the instruction.
5. Connect the power supply and switch on the 5. Refer to the instruction.
instrument.
6. Select REAGENT/PRIME ALL.
7. Verify the good working in check sensors.

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 REF : M60-SM-000 Rev 02

In Line Pressure Sensor Disassembly

In Line Pressure Sensor replacement

Tools/Material • NA

DISMANTLING
Action Step Reference

Prerequisite 1. Switch off the instrument and disconnect 1. Refer to the instruction.
the power supply.
2. Remove the fluidic door. 2. Refer to the instruction.
3. Remove the reagent door. 3. Refer to the instruction.
4. Remove the top cover. 4. Refer to the instruction.
5. Remove the reagent plate 5. Refer to the instruction.

Dismantling 1. Disconnect the two tubing #10B and #10C

from the sensor. 10B

10C

Dismantling 2. Disconnect the electrical connector.

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 REF : M60-SM-000 Rev 02

Dismantling 3. Remove the pressure sensor from the unit.

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 REF : M60-SM-000 Rev 02

In Line Pressure Sensor assembling

Tools/Material • NA

ASSEMBLING
Action Step Reference

Prerequisite 1. Follow the instruction of In Line Pressure 1. Refer to the instruction.

Sensor

Assembling 1. Connect the electrical connector.

Assembling 2. Connect the tubings #10B and #10C to

the sensor. 10B

10C

Completion 1. Install the reagent plate. 1. Refer to the instruction.

2. Install the top cover. 2. Refer to the instruction.
3. Install the reagent door. 3. Refer to the instruction.
4. Install the fluidic door. 4. Refer to the instruction.
5. Connect the power supply and switch on 5. Refer to the instruction.
the instrument.
6. Verify the good working in check
sensors screen
7. Select SERVICE/TECHNICIAN/ADJUST
OTHERS, Enter the altitude in dedicated
field, then press ADJUST.

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 REF : M60-SM-000 Rev 02

Optic Module Disassembly

Optic Module replacement.

Tools/Material • Torx tool size T20

DISMANTLING
Action Step Reference

Prerequisite 1. Perform a drain flow cell cycle. 1. Refer to the instruction.

2. Switch off the instrument and disconnect
the power supply. 2. Refer to the instruction.
3. Remove the fluidic door. 3. Refer to the instruction.
4. Remove the reagent door. 4. Refer to the instruction.
5. Remove the top cover. 5. Refer to the instruction.
6. Remove the reagent plate. 6. Refer to the instruction.

Dismantling 1. Disconnect the four connectors

from detection boards.

Dismantling 2. Disconnect the optic LED connector.

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Dismantling 3. Disconnect the diluent tubings (#35B &

#35C)
from the Y connector.

Dismantling 4. Disconnect the dilution tubings (#34A)

from the flow cell.

Dismantling 5. Locate plastic clip which maintain the tubing

#33E in good position.

NOTE: Position of tubing 33E is very

important, it allows keeping potential air
bubbles in the higher
part of the tubing thus avoiding pushing it up into
the
flow cell.

Dismantling 6. Remove tubing 33E from plastic clip.

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Dismantling 7. Disconnect tubing #33E from the Y

connector.

Dismantling 8. Disconnect tubing #36 from the check

valve.

Dismantling 9. Remove the ground cable fixation screw.

Dismantling 10. Release (do not remove) the optic module

fixations screws.

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 REF : M60-SM-000 Rev 02

Dismantling 11. Remove the optic module from the unit.

Completion NOTE: To avoid any risk of clotting, it is

recommended to dry the flow cell using an
air aerosol.

7. Connect the air aerosol to the tubing #35B

and dry it.

8. Connect the air aerosol to the tubing #35C

and dry it.

9. Connect the air aerosol to the tubing #33E

and dry it.

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 REF : M60-SM-000 Rev 02

Optic Module assembling

Tools/Material • Torx tool size T20

• Adjustable Torque screw tool

ASSEMBLING
Action Step Reference

Prerequisite 1. Follow the instruction of Optic 1. Refer to the instruction.

Module dismantling.

Assembling 1. Install the optic module on the unit.

Assembling 2. Secure the module with the fixation

screws tighten to dedicated torque.

20 cNm

Assembling 3. Install the ground cable fixation

screw tighten to dedicated torque.
150 cNm

Assembling 12. Connect tubing #36 on the check valve.

#36

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 REF : M60-SM-000 Rev 02

Assembling 13. Connect tubing #33E on the Y connector.

#33E

Assembling 14. Install tubing 33E on plastic clip.

#33E

Assembling 15. Notice the position of tubing #33E

and repeat.

NOTE: Position of tubing 33E is very

important. Allows keeping potential air
bubbles in the higher part of the tubing
thus avoiding pushing it up into the flow
cell.

Assembling 16. Connect tubings #34A on the flow cell.

#34A

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 REF : M60-SM-000 Rev 02

Assembling 17. Connect tubings #35B & #35C on the Y

connector.

#35c
#35B

Assembling 18. Connect the optic LED connector.

Assembling 19. Connect the four connectors on

detection boards.

NOTE: Yellow coaxial must be connected on

ALL board. Black coaxial must be connected
on FSC board.

Completion 1. Install the reagent plate 1. Refer to the instruction.

2. Install the top cover 2. Refer to the instruction.
3. Install the reagent door 3. Refer to the instruction.
4. Install the fluidic door 4. Refer to the instruction.
5. Connect the power supply and switch on 5. Refer to the instruction.
the instrument
6. Perform FLUIDIC CONTROL cycle. 6. Refer to the instruction.
7. Perform a startup cycle. 7. Refer to the instruction.
8. Follow the instruction of Optic Module 8. Refer to the instruction.
Adjustment.

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 REF : M60-SM-000 Rev 02

Optic Module Adjustment.

Tools/Material • Torx tool size T06

• Torx tool size T10
• Adjustable Torque screw tool
• Transverse adjustment tool
• Latex beads Ø7µm
• G-Cal

ASSEMBLING
Action Step Reference

Prerequisite 1. Follow the instruction of Optic 2. Refer to the instruction.

Module assembling.

1. Select: SERVICE/TECHNICIAN/ADJUST
WBC then select ADJ LED.

1. Select the green tick to validate the optic LED

Adjustment adjustment

2. After LED adjustment, new OPTIC LED and ALL

value are implemented

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3. When the optic LED adjustment is done, place a

tube of latex 7µm in sampling position then
press on CHECK OB.

4. After CHECK OB cycle completion, the latex

mark is displayed on screen.

NOTE: the latex mark must have the same shape

than the one on the picture of the right. If it is
Adjustment
the case go directly to the point 17 from this
instruction.

NOTE: If the latex mark has this shape it

means the transverse adjustment must be done.

1. If the latex mark does not have a correct

shape, remove the flow cell protection
cover fixation screw.

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 REF : M60-SM-000 Rev 02

2. Release (do not remove) the

transverse support fixation screw ½ turn

5. Press on ADJ.OB

Adjustment

6. The following window is displayed, select

the green tick to validate.

Adjustment 7. The following window is displayed

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8. Place the stopcock in close position.

Adjustment

9. Place a tube of latex 7µm in sampling

position then select the green tick to
validate

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 REF : M60-SM-000 Rev 02

Adjustment 10. When the curves appear on screen, you can begin
the adjustment turning the transverse
adjustment tool on both side.

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 REF : M60-SM-000 Rev 02

11. The goal is to adjust the transverse position to

have the 2 curves as flat as possible and as
close as possible from each other

12. Redo the adjustment as necessary until to

obtain the good result.

13. Once the transverse adjustment done, place

the stopcock in vertical position (open).

Adjustment

14. Place a tube of latex 7µm in sampling position

then press on CHECK OB.

15. After CHECK OB cycle completion, the latex

mark is displayed on screen.

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NOTE: the latex mark must have this shape to

consider the optic module as conform. If it is the
case go directly to Xx from this instruction.

16. When the latex mark is correct, screw

the transverse support fixation screw to lat e x m a rk conf o rm
the dedicated torque.

Adjustment
15 cNm

3. install the flow cell protection cover

fixation screw tighten to the dedicated
torque.

40 cNm

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 REF : M60-SM-000 Rev 02

17. Place a vial of G-Cal in sampling position then

press on ADJ.WBC.

18. At the end of the cycle FSC and ALL gain

values are updated
Adjustment

19. Run with fresh blood and verify the good

position of the scattergram.

Page 130/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02
CPU board

CPU board replacement.

Tools/Material • Torx tool size T10.

DISMANTLING INSTRUCTION
Action Step Reference

Prerequisite 1. Select SERVICE/BACKUP & 1.

RESTORE/BACKUP and follow the
instructions displayed.

NOTE: USB flash drive is mandatory to

backup. 2. Refer to the instruction.

3. Refer to the instruction.

2. Switch off the instrument and disconnect the

power supply.
3. Follow the instruction of Front Cover
Dismantling.

Dismantling 1. Remove the 7 CPU board fixation screws.

Dismantling 2. Remove the 6 remaining connectors from

CPU board.
3. Remove the CPU board from location

Page 131/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

CPU board assembling

Tools/Material • Torx tool size T10.

ASSEMBLING INSTRUCTION
Action Step Reference

Prerequisite 1. Follow the instruction of CPU board 1. Refer to the instruction.

dismantling.
2. Note the serial number of the new CPU
board.

Assembling 1. Install the CPU on location.

2. Connect the 6 cables to the CPU.

Assembling 3. Install the CPU board fixation screws.

Completion 1. Follow the instruction of Front Cover 1. Refer to the instruction.

Assembling.
2. Connect the main cord and switch on the 2. Refer to the instruction.
instrument.
3.
3. Select SERVICE/TECHNICIAN/
SYSTEM CONFIG, then enter the new
CPU serial number and instrument serial
number.

Page 132/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

4. Select
SERVICE/BACKUP&RESTORE/RESTORE 4.
and follow the instructions displayed.

NOTE: USE the same USB flash drive than

the one used to backup.

5.

5. Select SERVICE/TECHNICIAN/ADJUST
OTHERS, enter the altitude (where the
instrument is located) in the dedicated 6.
field and select ADJUST.

6. From the same screen, using Latex

particles 7µm, perform WBC gain
adjustment pressing on ADJUST.

NOTE: Compare the size of the latex

particles with the value in the field
DIAMETER and modify as necessary.
7.

7. From the same screen, using Latex

particles 5µm, perform RBC gain 8.
adjustment.

NOTE: Compare the size of the latex

particles with the value in the field 9.
DIAMETER and modify as necessary.

8. Select
SERVICE/TECHNICIAN/ADJUST
OTHERS, in the field HGB select
ADJUST.

9. Validate the adjustments by selection of

the green tick. 10.

10. Select
SERVICE/TECHNICIAN/ADJUST/ADJ.LE
D to
perform optic LED adjustment.

Page 133/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

11. Place a vial of G-Cal in sampling position 11.

then press on ADJ.WBC.

12. At the end of the cycle FSC and ALL gain 12.
values are updated

13. Run with fresh blood and verify the good 13.
position of the scattergram

Page 134/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02
USB board replacement.

Tools/Material • Torx tool size T10.

DISMANTLING INSTRUCTION
Action Step Reference

Prerequisite 1. Switch off the instrument and disconnect the 1. Refer to the instruction.
power supply.
2. Follow the instruction of Front Cover 2. Refer to the instruction.
Dismantling.

Dismantling 1. Disconnect the USB cable coming from CPU.

2. Remove the USB ground cable fixation screw.

Dismantling 3. Remove the 2 USB board fixation screws.

Dismantling 4. Remove the USB board from the instrument.

Page 135/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Tools/Material • Torx tool size T10.

ASSEMBLING INSTRUCTION
Action Step Reference

Prerequisite 1. Follow the instruction of USB board 2. Refer to the instruction.

dismantling.

Assembling 1. Connect the USB cable coming from CPU.

Assembling 2. Install the 2 USB board fixation screws.

3. Install the USB ground cable fixation
screw.

Completion 1. Follow the instruction of Front Cover 7. Refer to the instruction.

Assembling.

Using an USB flash drive, verify the good

working of the new USB board

Page 136/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Rear Panel board replacement

Rear Panel board dismantling.

Tools/Material • Torx tool size T10.

DISMANTLING INSTRUCTION
Action Step Reference

Prerequisite 1. Switch off the instrument and 1. Refer to the instruction.

disconnect the power supply.
2. Follow the instruction of reagent 2. Refer to the instruction.
plate dismantling.

Dismantling 1. Remove the 3 fixation screws of the

Rear Panel Board.

Dismantling 2. Disconnect the cables from the board.

Dismantling 3. Remove the board from the unit.

Page 137/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Rear Panel board assembling

Tools/Material • Torx tool size T10.

ASSEMBLING INSTRUCTION
Action Step Reference

Prerequisite 1. Follow the instruction of Rear Panel board 1. Refer to the instruction.
dismantling

Assembling 1. Connect the cables onto the board.

Assembling 2. Install the board with the fixation screws.

Completion 1. Follow the instruction of Reagent Plate 1. Refer to the instruction.

Assembling.
2. Connect the main cord and switch on the Refer to the instruction.
instrument.
Verify the good working of the associated board
function.

Page 138/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

HGB Board replacement

HGB Board dismantling.

Tools/Material • Torx tool size T10.

DISMANTLING INSTRUCTION
Action Step Reference

Prerequisite 1. Switch off the instrument and disconnect the 1. Refer to the instruction.
power supply.
2. Open the fluidic door. 2. Refer to the instruction.

Dismantling 4. Disconnect the 2 cables from the HGB board.

Dismantling 5. Remove the HGB board fixation screw, then

remove the board from the unit.

Page 139/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

HGB Board assembling

340B

Tools/Material
354B • Torx tool size T10.
354B

ASSEMBLING INSTRUCTION
Action Step Reference
3546B

3547B 3548B 3549B

Prerequisite
350B 1. Follow the instruction of HGB Board dismantling
351B 1. Refer to the
352B

instruction.

Assembling
35B 1. Install the board with the fixation screw.
354B

15cNm
35B

Assembling
356B 2. Connect the 2 cables connectors.
357B

Completion
358B 1. Connect the main cord and switch on the instrument.
359B 1. Refer to the instruction.
3562B

2. Close the fluidic board.

3560B

3. Select SERVICE/TECHNICIAN/ADJUST OTHERS,

3561B

2. Refer to the
356B

in the field HGB select ADJUST. instruction. 3.

3564B

Page 140/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Pump Disassembly

Pump replacement.
341B

Tools/Material
180B • Torx tool size T10

DISMANTLING
18B

Action
182B Step
183B Reference
184B

Prerequisite
185B 1.
186B Switch off the instrument and disconnect 1.
1893B Refer to the instruction.
the power supply.
2.
187B Remove the fluidic door. 2.
1894B Refer to the instruction.
3.
18B Remove the reagent door. 3.
1895B Refer to the instruction.
4.
189B Remove the top cover. 4.
1896B Refer to the instruction.
5.
1890B Remove the front cover. 5.
1897B Refer to the instruction.
6.
189B Remove the reagent plate. 6.
189B Refer to the instruction.
7.
1892B Remove the second reagent plate.

Dismantling
189B 1.
190B Remove the two fixation screws

190B

Page 141/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Dismantling
1902B 2.
1903B Disconnect the two tubings #10D & #23E
from the pump.
1904B

#23E
1905B
#10D

Dismantling
1906B 3.
1907B Disconnect the electrical connector.

Page 142/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Dismantling
1908B 4. Remove the pump from the unit.

Page 143/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Rinse pump assembling

342B

Tools/Material
190B • Torx tool size T20

ASSEMBLING
190B

Action
19B Step
192B Reference
193B

Prerequisite
194B
1.
195B Follow the instruction of the Rinse 1.
196B Refer to the instruction.
pump dismantling

Assembling
197B 5.
198B Connect the electrical connector.

Assembling
19B 6.
1920B Connect the two tubings #10C & #10B)
from the pump.

#10C
192B
#10B

#10C
1923B

#10B
NOTE: Pay attention to tubing connections
192B

on rinse pump.

Page 144/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

7.
1924B Install the pump with the two
fixation screws.

NOTE: No specific torque for these

1925B

screws, just screw until the mechanical

contact with the rubber washers, then
perform 1/4 turn more.

Completion
1926B 1. Install the second reagent plate.
1927B 1. Refer to the instruction.
1934B

2. Install the reagent plate.

1928B 2. Refer to the instruction.
1935B

3. Install the top cover.

192B 3. Refer to the instruction.
1936B

4. Install the reagent door.

1930B 4. Refer to the instruction.
1937B

5. Install the fluidic door.

193B 5. Refer to the instruction.
1938B

6. Connect the power supply and switch on

1932B 6. Refer to the instruction.
193B

the instrument
7. Verify the good working in check
193B

sensors screen.

Page 145/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Sampling Module Disassembly

Sampling probe replacement.

34B

Tools/Material
1940B
• Beak pliers

DISMANTLING
194B

Action
1942B Step
1943B Reference
194B

Prerequisite
1945B 1.
1946B Switch off the instrument and disconnect 1.
195B Refer to the instruction.
the power supply.
2.
1947B Remove the fluidic door. 2.
1952B Refer to the instruction.
3.
1948B Remove the reagent door. 3.
1953B Refer to the instruction.
4.
194B Remove the top cover. 4.
1954B Refer to the instruction.
5.
1950B Remove the front cover. 5.
195B Refer to the instruction.

Sampling
1956B 1.
1957B Pull on the top of the sampling probe and
Probe release it from the sampling probe
Dismantling carriage.

Sampling
1958B 2.
195B Pull on the rinse head to release it from
Probe the rocker module.
Dismantling

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 REF : M60-SM-000 Rev 02

Sampling
1960B 3.
196B Pull the sampling probe up until to remove
Probe it from the rinse head.
Dismantling

Sampling
1962B 4.
1963B Disconnect the tubing 9 from the
Probe sampling probe.
Dismantling

NOTE: Because tubing 9 material is

1964B

polyurethane, do not try to pull on it

to disconnect from the sampling
probe.
Use a beak pliers or an equivalent tool
1965B

as described below.

196B

Page 147/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Sampling
1967B 5.
1968B Insert the beak pliers or an equivalent tool
Probe in the space located between the base of
Dismantling the probe and the end of tubing 9.

NOTE: Move the tool to the down, leveraging

196B

on tubing 9 moving it through the top.

Sampling
1970B 6.
197B Once tubing 9 begins moving onto the
Probe probe, shift the tool transversely and do
Dismantling the action than on point 5.

7.
1972B Repeat the same operation until
the complete disconnection of
tubing 9.

NOTE: To prevent any risk of leakage, it

1973B

is recommended to replace the O-ring

located in the rinse head when replacing
the sampling probe.

Page 148/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Probe assembling
34B

Tools/Material
1974B
• Torx T06
•
1975B Cutting pliers

ASSEMBLING
1976B

Action
197B Step
1978B Reference
197B

Prerequisite
1980B 1.
198BFollow the instruction of sampling 1.
1983B Refer to the instruction.
probe dismantling.
2. Follow the instruction of O-ring
1982B
2.
1984B Refer to the instruction.
replacement.

Sampling 1. Using cutting pliers, refresh tubing 9

Tubing 9
1985B 1986B

Probe from 3 millimeters.

198B

Assembling

NOTE: To facilitate connection of tubing 9,

1987B

insert
Torx tool size T06 to enlarge internal Ø.
198B

Torx
190B

T06

Sampling
19B 3.
192B Connect tubing 9 to the probe leaving a
Probe space from one millimeter between end
Assembling of tubing and the probe base.

1mm
193B

Needle base
194B

Sampling
195B 4.
196B Insert the probe in the rinse head.
Probe
Assembling

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 REF : M60-SM-000 Rev 02

Sampling
197B 5.
198B Install the rinse head on the rocker.
Probe
Assembling

Sampling
19B 6. Engage the base of the probe on the probe
20B

Probe carriage, respecting the good positioning

Assembling of the probe.

Needle
20B

carriage
Needle base
NOTE: Notice the specific shape of the
203B

201B

probe base and the carriage.

Sampling
204B 7.
205B Press on the probe base to assure the
Probe good positioning on the carriage.
Assembling

Completion
206B 1. Install the front cover.
207B 1. Refer to the instruction.
2013B

2. Install the top cover.

208B 2. Refer to the instruction.
2014B

3. Install the fluidic door.

209B 3. Refer to the instruction.
2015B

4. Install the reagent door.

201B 4. Refer to the instruction.
2016B

5. Connect the power supply cord and

201B 5. Refer to the instruction.
2017B

switch on the instrument.

6. Perform a startup cycle.
201B 6.
2018B Refer to the instruction.

Page 150/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Probe’s O-ring replacement

345B

Tools/Material
2019B
• NA

DISMANTLING
20B

Action
201B Step
20B Reference
203B

Prerequisite
204B 1.
205B Switch off the instrument and disconnect 1.
203B Refer to the instruction.
the power supply.
2.
206B Remove the fluidic door. 2.
2031B Refer to the instruction.
3.
207B Remove the reagent door. 3.
203B Refer to the instruction.
4.
208B Remove the top cover. 4.
203B Refer to the instruction.
5.
209B Remove the front cover. 5.
2034B Refer to the instruction.

Probe’s O-
2035B 1.
2036B Pull on the top of the sampling probe to
ring release it from the sampling probe
Dismantling carriage.

Probe’s O-
2037B
2.
2038B Pull on the rinse head to release it from
ring the rocker.
Dismantling

Page 151/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Probe’s O-
2039B 3. Unscrew totally the probe guide and
204B

ring release it from the rinse head.

Dismantling

Probe’s O-
2041B 4. Pull the sampling probe up, removing at the
204B

ring same time the probe, the probe guide and Needle
the probe’s O-ring from the rinse head.
2043B

Dismantling

Guide
204B

O-ring
2045B

Probe’s O-
2046B NOTE: Be careful to the gasket located in
2047B

ring the rinse head. It must stay in position

Dismantling during probe guide and O-ring removal but
check it out its presence. Gasket
2049B

5.
2048B Remove the Probe’s O-ring from the probe.

Page 152/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Probe’s O-ring assembling

346B

Tools/Material
205B
• NA

ASSEMBLING
2051B

Action
205B Step
2053B Reference
2054B

Prerequisite
205B 1.
2056B Follow the instruction of Sampling probe’s 1.
2058B Refer to the instruction.
O-ring dismantling.
2057B

1.
2059B Install the probe’s O-ring on the
probe without removing the probe
guide.

Probe’s O-
206B 2.
2061B Once O-ring is installed on the probe,
ring the assy. is ready to be installed. Needle
2063B

Assembling

Guide
2064B

3.
206B Install the assy. On rinse head.

Page 153/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

4.
2065B Screw the probe guide on the rinse head.

NOTE: It is not necessary to overtighten

206B

the probe guide on the rinse head, gently

screw it until the mechanical stop.

Probe’s O-
2067B 5.
2068B Put back the rinse head in the rocker.
ring
Assembling

Probe’s O-
2069B 6.
207B Insert the base of the probe on the
ring probe carriage, respecting the good
Assembling positioning of the probe.

Needle
207B

carriage

NOTE: Notice the specific shape of

2071B

Needle base
2073B

the probe base and the carriage.

Page 154/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Probe’s O-
2074B 6.
2075B Press on the probe base to assure it is
ring well pressed in the carriage.
Assembling

Completion
2076B 1. Install the front cover.
207B 1. Refer to the instruction.
2084B

2. Install the top cover.

2078B 2. Refer to the instruction.
2085B

3. Install the fluidic door.

2079B 3. Refer to the instruction.
2086B

4. Install the reagent door.

208B 4. Refer to the instruction.
2087B

5. Connect the power supply cord and

2081B 5. Refer to the instruction.
208B

switch on the instrument.

6. Select SERVICE/SYSTEM INIT
208B

7. Perform a startup cycle.

2083B 7.
2089B Refer to the instruction.

Page 155/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Rocker replacement

Rocker dismantling.
347B

•
2091B Torx T10
Tools/Material
209B
•
209B Torx T20
•
2093B Cutting pliers

DISMANTLING
2094B

Action
2095B Step
2096B Reference
2097B

Prerequisite
2098B 1.
209B Switch off the instrument and disconnect 1.
2104B Refer to the instruction.
the power supply.
2.
210B Remove the fluidic door. 2.
2105B Refer to the instruction.
3.
210B Remove the reagent door. 3.
2106B Refer to the instruction.
4.
210B Remove the top cover. 4.
2107B Refer to the instruction.
5.
2103B Remove the front cover. 5.
2108B Refer to the instruction.

Sampling
2109B 1.
210B Pull on the top of the sampling probe and
Probe release it from the sampling probe
Dismantling carriage.

Sampling
21B 2.
21B Pull on the rinse head to release it from
Probe the rocker module.
Dismantling

Page 156/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Sampling
213B 3.
214B Pull the sampling probe up until to remove
Probe it from the rinse head.
Dismantling

Rocker 215B 4.
217B To avoid any damage during
Dismantling
216B operations, place the probe as shown
on picture.

218B

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 REF : M60-SM-000 Rev 02

Rocker219B 1.
21B Disconnect tubing 10 from the rinse head.
Dismantling
210B

Rocker21B 2.
214B Disconnect tubing 21 from the rinse
Dismantling
213B head, then remove the rinse head.

Rocker215B 3.
217B Remove tubing 10 & 21 from rocker’s
Dismantling
216B location.
218B

Rocker219B 4.
213B Remove the two (2) fixation screws of
Dismantling
2130B the rocker guide.

2
213B 1

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 REF : M60-SM-000 Rev 02

Rocker213B 5.
2135B Move the rocker Backward.
Dismantling
2134B

Rocker2136B 6.
2138B Rotate the rocker’s guide clockwise then pull
Dismantling
2137B on it to remove it.
2139B

Rocker2140B 7.
214B Move the sampling probe carriage down on
Dismantling
214B the middle of the rocker.

Rocker 8. Release (do not remove) the fixation screw

Half a
2143B 2145B

Dismantling of the lower pulley.

2146B

214B

Turn

1
2147B

Page 159/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Rocker2148B 9.
2150B Remove the three (3) fixation screws of
Dismantling
2149B the rocker protection, then remove it.

2
215B

3
215B 1

Rocker2153B 10. Manually move the rocker forward.

215B

Dismantling
2154B

Rocker2156B 11. Release (do not remove) the fixation screw

2158B

Dismantling
2157B of the belt tensioning system.

One Turn
2159B

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 REF : M60-SM-000 Rev 02

Rocker2160B 12. Remove the lower fixation screw of the

216B

Dismantling
216B metallic plate which maintains the rocker
on the ball bearing.

1
2163B

Rocker2164B 13. Release (do not remove) the second

216B

Dismantling
2165B fixation screw of the metallic plate which
maintains the rocker on the ball bearing One Turn
2167B

1
2168B

Rocker2169B 14. Push manually on the metallic plate to

217B

Dismantling
2170B rotate it.

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 REF : M60-SM-000 Rev 02

Rocker217B 15. Keeping the metallic plate rotated, pull

2174B

Dismantling
2173B on the rocker to disengage it from the
ball bearing.

Rocker2175B 16. Using cutting pliers, remove the plastic

217B

Dismantling
2176B fastener which maintains the sampling
probe sensor cable on the board.

1
2178B

Rocker2179B 18. Disconnect the sampling probe sensor cable.

218B

Dismantling
2180B

218B

Rocker2183B 17. Remove rocker module from the unit.

2185B

Dismantling
2184B

2186B

Page 162/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Rocker assembling.
348B

•
218B Adjustable Torque tool
Tools/Material
2187B
•
2189B Torx T10
•
2190B Torx T20
•
219B Cutting pliers

ASSEMBLING
219B

Action
2193B Step
2194B Reference
2195B

Prerequisite
2196B 1. Follow the instruction of rocker
2197B 1.
2198B Refer to the instruction.
dismantling.

Rocker
219B 1.
201B Grease the rocker gear.
Assembling
20B

20B

Rocker
203B 2.
205B Install the rocker.
Assembling
204B

NOTE: maintain the metallic plate

206B

rotated to insert the rocker onto the ball

bearing

Rocker
207B 3. Once the ball bearing is engaged in its
209B

Assembling
208B location, gently push on the rocker until
the metallic plate can rotate freely
through the down.

Page 163/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Rocker 210B 4.
21B Secure the plate, with the second fixation
Assembling
21B screw and tight both screw to the TORQUE = 80 cNm
213B

dedicated torque

2
214B

1
215B

Rocker 216B 5.
218B Move the rocker forward and backward
Assembling
217B to engage the two (2) gears in each
other.

NOTE: Do not force on the rocker to

219B

engage the two (2) gears. It could damage

the teeth.
Find the good positioning allowing the gearing.
20B

21B

Rocker 2B 6.
24B Install the rocker guide in position by
Assembling
23B tilting it.

25B

Rocker
26B

Page 164/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Assembling
27B 7.
28B Once in position, rotate the guide to the
horizontal position.
29B

Rocker
230B 8.
23B Secure the guide with the two (2)
Assembling
231B fixation screws.

NOTE: Do not tight at this stage.

23B

2
1
234B

Rocker
235B 9.
237B Move the rocker over the guide.
Assembling
236B

Rocker
238B 10. Push up the guide against the rocker.
240B

Assembling
239B

NOTE: Pay attention to the guide

241B

positioning. It mustn’t be too high, it

could generate excessive friction with the
rocker.
Set a gap of one (1) millimeter between
24B

the guide and the rocker.

Front view
243B

1mm
24B

Page 165/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

1mm
245B

Rocker
246B 11. Secure the two (2) screws to the
248B

TORQUE = 80 cNm
250B

Assembling
247B dedicated torque.

2
251B

1
25B

NOTE: After tightening the fixation

249B

screws of the guide, move the rocker back

and forth and verify there is no mechanical
hard point.
3
253B 1

Rocker
254B 18. Manually move the rocker forward.
256B

Assembling
25B

Page 166/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Rocker
257B 19. Using adjustable torque tool, secure the
259B

Assembling
258B fixation screw of the belt tensioning
system to the dedicated torque.

3 cNm
260B

3 cNm
261B

Rocker
26B 20. Using adjustable torque tool, secure
264B

Assembling
263B the fixation screw of the lower pulley 20 cNm
265B

to the dedicated torque.

Page 167/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Rocker
26B 21. Once lower pulley fixation screw secured to
268B

Assembling
267B 20 cNm, apply the same torque of 20 cNm
269B

to the fixation screw of the belt tensioning

system.

20 cNm
270B

Rocker
271B 12. Pass tubing 21 in the first fastener.
273B

Assembling
27B

1
274B

Page 168/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Rocker
275B 13. Pass tubing 21 in the second fastener, then
27B

Assembling
276B in the dedicated place of the rocker.

NOTE: Hemostat can be used to

278B

facilitate the operation.

2
279B

3
280B

4
281B

Rocker
28B 14. Refresh tubing 21 from 3 millimeters,
284B

Assembling
283B then connect it to the rinse head.

Rocker
285B 15. Pass tubing 10 in the first fastener.
287B

Assembling
286B

1
28B

Rocker
289B 16. Pass tubing 10 in the second fastener, then
291B

Assembling
290B in the dedicated place of the rocker.
2
293B

NOTE: Hemostat can be used to

29B

facilitate the operation.

3
294B

4
295B

Page 169/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Rocker
296B 17. Pass tubing 10 in the dedicated hole of
298B

Assembling
297B the rocker.

NOTE: Refresh tubing 21 from 3

29B

millimeters before to connect. 55 mm

2301B

NOTE: Respect a length of 55mm between

230B

the end of tubing 10 and the hole on the

rocker.

Rocker
230B 18. Connect tubing 10 to the rinse head.
2304B

Assembling
230B

NOTE Once connected, tubing 10 must

2305B

make a loop as shown on the right picture.

NOTE: if the length of 55mm is not

2306B

respected (too short) the tubing 10 can be

pinched at the rinse head.

NOTE: If the length of 55mm is not

2307B

respected
(too long) the loop of tubing 10 is too large.
2308B

Page 170/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

In this configuration, tubing 10 can be

2309B

pinched against the front plate during

rocker movements.

NOTE: When the length of 55mm is

2310B

respected rocker can move freely without

risk of tubing 10 pinched with the front
plate.

Completion
231B 1. Install the front cover.
231B 1. Refer to the instruction.
2319B

2. Install the top cover.

231B 2. Refer to the instruction.
230B

3. Install the fluidic door.

2314B 3. Refer to the instruction.
231B

4. Install the reagent door.

2315B 4. Refer to the instruction.
23B

5. Connect the power supply cord and

2316B 5. Refer to the instruction.
23B

switch on the instrument.

6. Select SERVICE/SYSTEM INIT
2317B

7. Perform a startup cycle.

2318B
7.
234B Refer to the instruction.

Page 171/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Probe belt & probe carriage replacement

Probe belt & probe carriage dismantling

349B

• Torx T08
Tools/Material
236B

• Torx T10
235B

237B

Removal
238B

Action
239B Step
230B Reference
231B

Prerequisite
23B
1
23B Follow the instruction of Rocker 1.
234B Refer to the instruction.
dismantling.

Probe belt 235B 2. Remove the screw which maintains

239B

& 236B
the metallic shaft in location.
Carriage 237B

Dismantling
238B

1
2340B

Probe belt 2341B 3. Remove the metallic shaft from the rocker.
234B

& Carriage
234B

Dismantling

Page 172/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Probe belt
234B 4. Remove the fixation screw of the
2346B

& Carriage
2345B lower pulley.
Dismantling

1
2348B

NOTE: Be careful to the scare nut located

2347B

at the back of the rocker while removing the

lower pulley fixation screw.

Probe belt
2349B 5. Release the lower pulley from its location.
2351B

& Carriage
2350B

Dismantling

1
235B

Page 173/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Probe belt
235B 6. Remove the sub-assembly belt/carriage
235B

& Carriage
2354B from the rocker module.
Dismantling

2356B

Probe belt
2357B 7. Remove the two (2) fixation screws
2359B

& Carriage
2358B which maintain the belt in the carriage
Dismantling gripper.
1
2360B

2
2361B

Probe belt
236B 8. Release the belt from the carriage gripper.
2364B

& Carriage
236B

Dismantling

Page 174/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Probe belt
2365B 9. At this stage of the instruction, it is
2367B

& Carriage
236B possible to change either the belt or
Dismantling carriage.

Page 175/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Belt and probe carriage assembling

350B

•
2369B Torx T08
Tools/Material
2368B
•
2370B Torx T10
•
2371B Torx T20
•
237B Cutting pliers
•
237B Beak pliers

Removal
2374B

Action
2375B Action
2376B Action
237B

1.
2379B Follow the instruction of Probe belt & 1.
2380B Refer to the instruction.
Prerequisite
2378B
probe carriage dismantling.

Probe Belt
2381B
2
238B Install the probe carriage on the belt.
& Carriage
Assembling

238B

Page 176/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Probe Belt
2384B 1.
2385B Secure the assy. with the two (2)
& Carriage fixation screws.
Assembling
2
238B 1

Screw to 2389B

the
mechanical
NOTE : There is no specific torque, screw
2386B
stop
until the mechanical contact with the probe
carriage then stop screwing.

GOOD
2390B

NOTE: Do not overtight the screws, it

2387B
BAD
2391B

could damage the gripper of the

carriage.

Gripper239B

Is bent
239B

Probe Belt
2394B 2. Grease the groove of the rocker.
2395B

& Carriage
Assembling NOTE: The groove allows the straight
2396B

driving of the probe carriage during the up

and down movements, it must be greased.

2397B

Page 177/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Probe Belt
2398B 3. Install the assembly Belt/carriage.
239B

& Carriage
Assembling

240B

Probe Belt
2401B 4. Install the probe carriage in the groove.
240B

& Carriage
Assembling

Probe Belt
2403B
2. Install the down pulley in the belt
240B

& Carriage tension system.

Assembling

Probe Belt 3. Secure the assembly with the fixation

1
2405B 2406B

& Carriage screw to maintain elements together.

2407B

Assembling

Page 178/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Probe Belt
2408B 4. Place the square nut in position and screw
2409B

& Carriage by few turns.

Assembling

Probe Belt
2410B 5.
241B Install the metallic shaft inside the rocker.
& Carriage
Assembling

NOTE: Inserted the shaft first in the

241B

dedicated hole located on the top of the

rocker.

Then in the hole of the probe carriage.

2413B

2415B

Finally, in the bottom of the probe carriage.

241B

Page 179/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Probe Belt
2416B
6.
2417B Secure the shaft with the fixation screw.
& Carriage
Assembling NOTE: Move the washer onto the shaft

direction
before tightening to the dedicated torque.
2418B

Completion
2419B 1.
240B Follow the instruction of the
Rocker assembling.

Probe’s sensor board replacement

351B

Tools/Material
241B • Torx T08

Dismantling
24B

Action
243B Action
24B Action
245B

Prerequisite
246B 1.
247B Follow the instruction of Rocker dismantling. 1.
248B Refer to the instruction.

Probe’s 249B 1.
2430B Remove the two (2) fixation screws of
Sensor the probe sensor board. 1
2431B

Board
Dismantling

2
243B

Probe’s 243B 2.
243B Remove the probe sensor board from
Sensor the rocker.
Board
Dismantling

2435B

Page 180/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Probe’s sensor board assembling

352B

Tools/Material
2436B
• Torx T08

Assembling
2437B

Action
2438B Action
2439B Action
240B

Prerequisite
241B 1.
24B Follow the instruction of Probe’s sensor 1.
24B Refer to the instruction.
board dismantling
243B

Probe’s
245B 1.
246B Install the probe sensor board on the
1
Sensor rocker with the two (2) fixation screws
247B

Board tighten to the dedicated torque

Assembling

2
TORQUE = 80 cNm
248B

Probe’s
249B 2. Connect the cable to the probe sensor
2450B

Sensor board.
Board
Assembling

Probe’s
2451B 3.
245B Place a plastic fastener to maintain the
Sensor cable to the board
Board
Assembling

Completion
2453B 2.
245B Follow the instruction of the 1.
245B Refer to the instruction.
Rocker assembling.

Page 181/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Probe stepper motor replacement

35B

Tools/Material
2456B
• Torx T10

Dismantling
2457B

Action
2458B Action
2459B Action
2460B

Prerequisite
2461B 1.
246B Follow the instruction of Rocker dismantling. 1.
2463B Refer to the instruction.

Probe 246B 1.
2465B From fluidic modules side, locate the two (2)
Stepper fixation screws of the sampling probe motor.
246B

Motor 2
2467B

Dismantling

1
2468B

Probe 2469B 2. Remove the two (2) fixation screws of

2470B

2
Stepper the sampling probe motor.
247B

Motor
Dismantling NOTE: Hold the motor while fixation
2471B

screws removing.

1
2473B

Probe 247B
3. From tubing side, disconnect the
2475B

Stepper sampling probe motor connector.

Motor
Dismantling 4. Remove the sampling probe motor from
2476B

the unit.

1
247B

Page 182/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Probe stepper motor assembling

354B

Tools/Material
2478B
• Torx T10

Assembling
2479B

Action
2480B Action
2481B Action
248B

Prerequisite
2483B 1.
248B Follow the instruction of the Probe 2.
2485B Refer to the instruction.
stepper motor dismantling.

Probe 2486B 1. Connect the cable to the stepper motor.

2487B

Stepper
Motor
Assembling
1
248B

Probe 2489B 2. Install the stepper motor in position.

2490B

Stepper
Motor
Assembling NOTE: Connector must be oriented through
2491B

the top of the unit.

Probe 249B 3. Secure the motor with the two (2)

2493B

Stepper fixation screws tighten to the dedicated

Motor torque.
Assembling
1
249B

2
2495B

TORQUE = 120 cNm

2496B

Completion
2497B 1. Follow the instruction of the
2498B 1.
250B Refer to the instruction.
rocker assembling.
2. Select SERVICE/SYSTEM INIT
249B

Page 183/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Rocker stepper motor replacement

Rocker stepper motor dismantling

35B

•
250B Torx T10
Tools/Material
2501B
•
2503B Torx T20
•
2504B Cutting pliers
•
250B Beak pliers

Dismantling
2506B

Action
2507B Action
2508B Action
2509B

Prerequisite
2510B 1.
251B Switch off the instrument and disconnect 1.
2516B Refer to the instruction.
the power supply.
2.
251B Remove the fluidic door. 2.
2517B Refer to the instruction.
3.
2513B Remove the reagent door. 3.
2518B Refer to the instruction.
4.
2514B Remove the top cover. 4.
2519B Refer to the instruction.
5.
251B Remove the front cover. 5.
250B Refer to the instruction.

Rocker
251B 1.
25B Place the rocker in central position.
Stepper
Motor
Dismantling

2.
253B Remove the two (2) fixation screws of
the rocker guide.

25B

NOTE: protection can be installed to

254B

avoid screws falling in counter

chambers.

256B

Page 184/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Rocker
257B 3.
258B Release (do not remove) the higher
Stepper fixation screw of the holder plate.
Motor
Dismantling

4.
259B Remove the lower fixation screw of
the holder plate.

Rocker
2530B 5.
2531B Disengage the rocker from its location.
Stepper
Motor
Dismantling

253B

Rocker
253B 6.
2534B Place the rocker as shown on the right.
Stepper
Motor
Dismantling

253B

Page 185/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Rocker
2536B 1.
2537B From fluidic side, remove the two (2)
Stepper fixation screws of the rocker motor.
Motor
2
Dismantling
2539B

1
2540B

NOTE: Hold the motor while fixation

2538B

screws removal

Rocker
2541B 2. From tubing side, disconnect the rocker
254B

Stepper motor connector and remove the rocker

Motor motor from the unit.
Dismantling

Page 186/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Rocker stepper motor assembling

356B

•
254B Torx T10
Tools/Material
2543B
•
254B Torx T20

Assembling
2546B

Action
2547B Action
2548B Action
2549B

Prerequisite
250B 1.
251B Follow the instruction of Rocker 3.
25B Refer to the instruction.
stepper motor dismantling.

Rocker
253B 1.
254B Connect the cable to the stepper motor.
Stepper
Motor
Assembling

259B

2. Install the stepper motor in position.

25B

NOTE: Connector must be oriented through

256B

the front of the unit.

3.
257B Secure the motor with the two (2) fixation.

2
NOTE: Do not tighten at this stage.
2560B

258B

1
2561B

Page 187/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Rocker
256B 1.
2563B Follow the instruction of the 1. Refer to the instruction.
2564B

Stepper rocker assembling.

Motor
Assembling

Adjustment
256B 1.
256B Once the motor in position push it up until
the mechanical stop against the rocker
gear.

NOTE: Do not push up the motor too much,

2567B

the goal is to engage the teeth of motor and

rocker gears each together.

Adjustment
2568B 1.
2569B Tighten the two (2) screws to the
dedicated torque.

Page 188/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Rocker optical sensor replacement

357B

• Torx T10
Tools/Material
2571B

• Torx T20
2570B

257B

Dismantling
2573B

Action
2574B Action
257B Action
2576B

Prerequisite
257B 1. Switch off the instrument and disconnect
2578B 1.
258B Refer to the instruction.
the power supply.
2. Remove the reagent door.
2579B 2.
2583B Refer to the instruction.
3. Remove the top cover
2580B 3.
2584B Refer to the instruction.
4. Remove the Reagent plate.
2581B 4.
258B Refer to the instruction.

Rocker
2586B 1.
2587B Locate the rocker optical sensor on
Sensor tubing side.
Dismantling

Rocker
258B 1.
2589B Remove the rocker sensor unscrewing
Sensor the two (2) fixation screws.
Dismantling
1
2590B

2
2591B

Page 189/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

NOTE: Fixation screws are equipped with

259B

plastic washer. 1
2593B

2
2594B

Rocker
259B 2.
2596B Disconnect the sensor connector.
Sensor
Dismantling

Rocker
2597B 3.
2598B Remove the sensor from the unit.
Sensor
Dismantling

Page 190/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Rocker optical sensor assembling

358B

• Torx T10
Tools/Material
260B

• Torx T20
259B

2601B

Assembling
260B

Action
2603B Action
2604B Action
2605B

Prerequisite
260B 1.
2607B Follow the instruction of Rocker 1.
2608B Refer to the instruction.
optical sensor disassembling.

Rocker
2609B 1.
2610B Install the sensor in position.
Sensor
Assembling

Rocker
261B 2.
261B Secure the sensor with the fixation
1
Sensor screws tighten to the dedicated torque.
2613B

Assembling
2
2614B

Rocker
2615B 3.
261B Connect the sensor connector.
Sensor
Assembling

Page 191/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Completion
2617B 2. Follow the instruction of the
2618B 2. Refer to the instruction.
260B

rocker assembling.
3. Select SERVICE/SYSTEM INIT
2619B

Page 192/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Stopcock Disassembly

The following instructions will guide you to dismantle and assemble the stopcock element.
261B

Stopcock replacement.
359B

Tools/Material
26B •Torx tool size T20
• Cutting pliers
263B

DISMANTLING
264B

Action
265B Step
26B Reference
267B

Prerequisite
268B
1.
269B Switch off the instrument and disconnect 1.
2634B Refer to the instruction.
the power supply.
2.
2630B Remove the fluidic door. 2.
2635B Refer to the instruction.
3.
2631B Remove the reagent door. 3.
263B Refer to the instruction.
4.
263B Remove the top cover. 4.
2637B Refer to the instruction.
5.
263B Remove the reagent plate 5.
2638B Refer to the instruction.

Dismantling
2639B 1.
2640B Locate the stopcock at the back on the top
of the instrument.

Dismantling
2641B 2.
264B Using cutting pliers, remove the two
fasteners which maintain the
stopcock.

Page 193/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Dismantling
2643B 3.
264B Unscrew the Luer connector of the tubing
33B.
2645B

#33B
264B

Dismantling
2647B 4. Unscrew the Luer connector of the tubing
2648B

3C.

#3C
2649B

Dismantling
2650B 5.
2651B Remove the stopcock from the unit.

265B

Page 194/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Stopcock assembling
360B

Tools/Material
2653B • Torx tool size T20

ASSEMBLING
2654B

Action
265B Step
265B Reference
2657B

Prerequisite
2658B
1.
2659B Follow the instruction of 1.
260B Refer to the instruction.
Stopcock dismantling.
.
261B

Assembling
26B 1.
263B Install the Luer connector of the tubing 3C.

#3C
264B

Assembling
265B 2.
26B Unscrew the Luer connector of the tubing
33B.
267B

#33B
268B

Assembling
269B 6.
2670B Install two fasteners to maintain
the stopcock.

Page 195/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Completion
2671B 1. Install the reagent plate 1. Refer to the instruction.
267B

2. Install the top cover

267B
2. Refer to the instruction.
2678B

3. Install the reagent door

2673B
3. Refer to the instruction.
2679B

4. install the fluidic door

2674B
4. Refer to the instruction.
2680B

5. Connect the power supply and switch on

2675B
5. Refer to the instruction.
2681B

the instrument
6. Perform a fluidic control cycle.
267B
6.
268B Refer to the instruction.

Page 196/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Syringe Module

Syringe body & Pistons replacement.

361B

Tools/Material
2683B • Torx tool size T20

DISMANTLING
2684B

Action
2685B Step
268B Reference
2687B

Prerequisite
268B 1.
2689B Drain the instrument from any liquids. 1. Remove pick up tubings from their
2695B

container and select

REAGENT/PRIME ALL.
2.
2690B Switch off the instrument and disconnect 2. Refer to the instruction.
269B

the power supply.

3.
2691B Remove the fluidic door. 3.
2697B Refer to the instruction.
4.
269B Remove the reagent door. 4.
2698B Refer to the instruction.
5.
2693B Remove the reagent plate. 5.
269B Refer to the instruction.
6.
2694B Remove the top cover. 6.
270B Refer to the instruction.

Syringe2701B 1.
2704B Remove the four fixation screws of
Body 270B the syringe body.
& Pistons
2703B

Dismantling

Syringe2705B 2.
2708B Remove the assembly body/pistons.
Body 2706B

& Pistons
270B

Dismantling

2709B

Page 197/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Syringe
2710B
3.
2713B Remove the pistons from the syringe body.
Body 271B

& Pistons
271B

Dismantling

Page 198/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Syringe body & pistons assembling

362B

Tools/Material
2714B • Torx tool size T20

ASSEMBLING
2715B

Action
2716B Step
271B Reference
2718B

Prerequisite
2719B 1.
270B Follow the instruction of Syringe body & 1.
27B Refer to the instruction.
pistons dismantling.
271B

.
273B

Syringe Body 1.
274B 276B Install the pistons in the syringe body.
& Pistons 275B

Assembling

Sampling Piston
Diluent Piston

Waste Piston

Waste Piston

Lyse Piston
2731B
279B

2730B
278B
27B

Syringe Body 2. Install the assembly on the syringe

273B 2734B

& Pistons motor holder.

273B

Assembling
Note: Be sure all the 16 O-rings are in well
2735B

in good position before installing the syringe

body.

2736B

Page 199/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

3. Install the body on the syringe motor

273B

holder.

NOTE: Pay attention to the good positioning

274B

2738B

of the 5 pistons in the carriage before to

secure the module with fixation screws.

4. Secure the body with the six fixations

2739B

screws.

NOTE: Tighten first the six screws to

2740B
35cNm
2743B

35 cNm respecting the order.

60cNm
274B

NOTE: Tighten the screws from the top

2741B

to 60 cNm.

Page 200/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Completion
2745B 1. Install the fluidic door. 1. Refer to the instruction.
275B

2. Install the reagent door.

2746B
2. Refer to the instruction.
2753B

3. Install the top cover

274B
3. Refer to the instruction.
2754B

4. Connect the power supply and switch on

2748B
4. Refer to the instruction.
275B

the instrument.
5. Select
2749B

SERVICE/TROUBLESHOOTING/SYRINGE
GREASING and perform a pistons greasing.
6. Select
2750B

SERVICE/TROUBLESHOOTING/CHECK
SENSORS/TEST VACUUM and control
the stability of the vacuum in waste
pressure field.
7. Select REAGENT/PRIME ALL.
2751B

Page 201/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Syringe motor holder replacement.

36B

Tools/Material
2756B
• Torx tool size T20

DISMANTLING
275B

Action
2758B Step
2759B Reference
2760B

Prerequisite
2761B 1. Drain the instrument from any liquids.
276B 1.
270B Refer to the instruction.
2. Switch off the instrument and disconnect
2763B

the 2.
271B Refer to the instruction.
power supply.
2764B
3.
27B Refer to the instruction.
3. Remove the fluidic door.
2765B
4.
273B Refer to the instruction.
4. Remove the reagent door.
276B
5.
274B Refer to the instruction.
5. Remove the top cover.
276B
6.
275B Refer to the instruction.
6. Follow the instruction of Syringe body &
2768B

pistons dismantling

Syringe
276B 1.
27B Remove the ground cable fixation screw.
motor
holder
Dismantling

Syringe
278B 2.
279B Disconnect the sensor cable connector.
Motor
Holder
Dismantling

Syringe
2780B 3.
2781B Disconnect the motor cable connector.
Motor
Holder
Dismantling

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 REF : M60-SM-000 Rev 02

Syringe
278B 4.
2783B Disconnect all tubings from Syringe
Motor motor holder
Holder
Dismantling

2784B

2785B

Syringe
2786B 5.
278B Move the piston carriage downward.
Motor
Holder
Dismantling

Page 203/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Syringe
278B 5.
2789B Release (do not remove) the four
Motor fixation screws
Holder
Dismantling

Syringe
2790B 6.
2791B Remove the syringe motor holder from
Motor the unit.
Holder
Dismantling

279B

Page 204/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Syringe motor holder assembling

364B

Tools/Material
2793B • Torx tool size T20

ASSEMBLING
2794B

Action
2795B Step
2796B Reference
279B

Prerequisite
2798B
1.
279B Follow the instruction of Syringe 1.
280B Refer to the instruction.
motor holder dismantling.
.
2801B

Syringe
280B 1.
2803B Place the syringe motor holder in position.
Motor
Holder
Assembling

2804B

Syringe
2805B 2.
2806B Secure the module with the four
Motor fixation screws, tighten to the
Holder dedicated torque.
Assembling

20 cNm
2807B

Syringe
280B 3.
2809B Connect all tubings on syringe motor holder
Motor
Holder
Assembling

2810B

Page 205/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Syringe
281B 6.
281B Connect the motor cable connector.
Motor
Holder
Assembling

Syringe
2813B 7.
2814B Connect the sensor cable connector.
Motor
Holder
Assembling

Page 206/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Syringe
2815B 8.
2816B Install the ground cable fixation
Motor screw tighten to the dedicated
Holder torque. 150 cNm
2817B

Assembling

Completion
281B 1. Connect the power supply and switch on
2819B 1.
28B Refer to the instruction
the instrument.
2. Follow the instruction of Syringe body &
280B 2.
283B Refer to the instruction
pistons assembling.
281B

Page 207/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Slotted optical sensor replacement.

365B

Tools/Material
284B
• Torx tool size T10

DISMANTLING
285B

Action
286B Step
287B Reference
28B

Prerequisite
289B 1.
2830B Switch off the instrument and disconnect 1.
2835B Refer to the instruction.
the power supply.
2.
2831B Remove the reagent door. 2.
2836B Refer to the instruction.
3.
283B Remove the fluidic door. 3.
2837B Refer to the instruction.
4.
283B Remove the reagent plate. 4.
283B Refer to the instruction.
5.
2834B Remove the top cover. 5.
2839B Refer to the instruction.

Slotted
2840B 1.
2841B Disconnect the syringe sensor connector.
optical
sensor
Dismantling

Slotted
284B 2.
2843B Remove the syringe sensor fixation screw.
optical
sensor
Dismantling

Page 208/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

4. Remove the syringe sensor from the unit.

Slotted
2845B

284B

optical
sensor
Dismantling

Page 209/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Slotted optical sensor assembling

36B

Tools/Material
2846B • Torx tool size T10

ASSEMBLING
2847B

Action
284B Step
2849B Reference
2850B

Prerequisite
2851B
1.
285B Follow the instruction of Slotted 1.
2853B Refer to the instruction.
optical sensor dismantling.

Slotted 2854B 1.
285B Install the syringe sensor on the
optical motor holder.
sensor
Assembling

2856B

Slotted 2857B 2.
285B Secure the syringe sensor with the
optical fixation screw tighten to the dedicated
sensor torque.
Assembling
100 cNm
2859B

Syringe
2860B 5.
2861B Connect the syringe sensor connector.
optical
sensor
Assembling

Page 210/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Completion
286B 1. Install the reagent plate 1. Refer to the instruction
286B

2. Install the top cover.

2863B
2. Refer to the instruction
2869B

3. Install the fluidic door.

2864B
3. Refer to the instruction
2870B

4. Install the reagent door.

2865B
4. Refer to the instruction
2871B

5. Connect the power supply and switch on the

286B
5. Refer to the instruction
287B

instrument.
6.
2867B Select
SERVICE/TROUBLESHOOTING/CHECK
SYRINGE to control the good working of
the new sensor

Page 211/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Syringe Valves module

Syringe Valves module replacement.

367B

Tools/Material
2873B
• Torx tool size T20

DISMANTLING
2874B

Action
2875B Step
2876B Reference
287B

Prerequisite
287B 1.
2879B Drain the instrument from any liquids. 1. Remove pick up tubings from their
285B

containers and select REAGENT/

PRIME ALL.
2.
280B Switch off the instrument and disconnect 2. Refer to the instruction.
286B

the power supply.

3.
281B Remove the fluidic door. 3.
287B Refer to the instruction.
4.
28B Remove the reagent door. 4.
28B Refer to the instruction.
5.
283B Remove the top cover. 5.
289B Refer to the instruction.
6.
284B Remove the reagent plate 6.
2890B Refer to the instruction.

Dismantling
2891B 1.
289B Disconnect the tubings from the module.

2893B

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 REF : M60-SM-000 Rev 02

Dismantling
2894B 2.
2895B Disconnect the valves connectors.

Dismantling
2896B 3.
2897B Remove the four fixation screws.

4.
289B Remove the Syringe Valves module from
the unit.

Page 213/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Syringe Valves module assembling

368B

Tools/Material
289B • Torx tool size T20

ASSEMBLING
290B

Action
2901B Step
290B Reference
2903B

Prerequisite
2904B
1.
2905B Follow the instruction of Syringe 1.
2906B Refer to the instruction.
Valves module #3.

1. Install the Syringe Valves module.

2908B

Assembling
2907B
2. Secure the assembly with the four
290B

fixation screws tighten to the dedicated

100 cNm
torque.
2910B

Assembling
291B 3.
291B Connect the tubings to the assembly.

2913B

2914B

Completion
2915B 1. Install the reagent plate.
2916B 1.
29B Refer to the instruction.
2. Install the top cover.
2917B

3. Install the fluidic door.

2918B
2.
293B Refer to the instruction.
4. Install the reagent door.
291B
3.
294B Refer to the instruction.
5. Connect the power supply and Switch on
290B
4.
295B Refer to the instruction.
the instrument. 5.
296B Refer to the instruction.
6. Perform a prime all cycle.
291B
6.
297B Refer to the instruction.

Page 214/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Ambient Temperature Sensor Disassembly

Ambient Temperature Sensor Replacement.

369B

Tools/Material
298B
• Torx tool size T10

DISMANTLING
29B

Action
2930B Step
2931B Reference
293B

Prerequisite
293B 1.
2934B Switch off the instrument and disconnect 1.
2941B Refer to the instruction.
the power supply.
2.
2935B Remove the fluidic door. 2.
294B Refer to the instruction.
3.
2936B Remove the reagent door. 3.
2943B Refer to the instruction.
4.
2937B Remove the top cover. 4.
294B Refer to the instruction.
5.
2938B Remove the front cover. 5.
2945B Refer to the instruction.
6.
293B Remove the reagent plate. 6.
2946B Refer to the instruction.
7.
2940B Remove the second reagent plate.

Dismantling
2947B 1.
2948B Locate the instrument temperature
sensor screwed on the base pate.

Dismantling
294B 2.
2950B Remove the fixation screw.

3.
2951B Remove the temperature sensor from
the unit.

Page 215/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

NOTE: Ambient temperature sensor is

295B

directly connected to the CPU board

Page 216/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Ambient temperature sensor assembling

370B

Tools/Material
2953B • Torx tool size T10

ASSEMBLING
2954B

Action
295B Step
2956B Reference
2957B

Prerequisite
2958B
1.
295B Follow the instruction of 1.
2960B Refer to the instruction.
Ambient temperature sensor
dismantling.

Assembling
2961B 1.
296B Install the ambient temperature sensor
with the fixation screw tighten to the
dedicated torque.

NOTE: Ambient temperature sensor is

2963B

directly connected to the cpu board.

2964B

150 cNm
2965B

Completion
296B 1. Install the second reagent plate.
2967B 1. Refer to the instruction.
2976B

2. Install the reagent plate.

2968B 2. Refer to the instruction.
297B

3. Install the top cover.

296B 3. Refer to the instruction.
2978B

4. Install the front cover.

2970B 4. Refer to the instruction.
297B

5. Install the reagent door.

2971B 5. Refer to the instruction.
2980B

6. Install the fluidic door.

297B 6. Refer to the instruction.
2981B

7. Connect the power supply and switch on

2973B

the instrument.
8. Verify the good working of the sensor
2974B

in check screen.
9. Verify the good working of the
2975B

ambient temperature sensor in check

sensors.

Page 217/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Diluent temperature sensor replacement.

Diluent temperature sensor dismantling.

371B

Tools/Material
298B
• Torx tool size T10

DISMANTLING
2983B

Action
2984B Step
2985B Reference
2986B

Prerequisite
2987B 1.
298B Switch off the instrument and disconnect 1.
295B Refer to the instruction.
the power supply.
2.
298B Remove the fluidic door. 2.
296B Refer to the instruction.
3.
290B Remove the reagent door. 3.
297B Refer to the instruction.
4.
291B Remove the top cover. 4.
298B Refer to the instruction.
5.
29B Remove the front cover. 5.
29B Refer to the instruction.
6.
293B Remove the reagent plate. 6.
30B Refer to the instruction.
7.
294B Remove the second reagent plate. 7.
301B Refer to the instruction.

Dismantling
302B 1.
30B Locate the diluent temperature sensor
close to the back of WBC chamber.

Dismantling
304B 2.
305B Remove the fixation screw.

Page 218/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Dismantling
306B 3.
307B Disconnect the temperature sensor.

Dismantling
308B 4.
309B Remove the temperature sensor from
the unit.

301B

Page 219/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Diluent temperature sensor assembling

372B

Tools/Material
301B • Torx tool size T10

ASSEMBLING
3012B

Action
301B Step
3014B Reference
3015B

Prerequisite
3016B
1.
3017B Follow the instruction of Diluent 1.
3018B Refer to the instruction.
temperature sensor
dismantling.

Assembling
3019B 1.
302B Install the diluent temperature sensor
with the fixation screw tighten to the
100 cNm
dedicated torque.
3021B

Assembling
302B 2.
302B Connect the diluent temperature sensor.

Completion
3024B 1. Install the second reagent plate.
3025B 1. Refer to the instruction.
30B

2. Install the reagent plate.

3026B 2. Refer to the instruction.
304B

3. Install the top cover.

3027B 3. Refer to the instruction.
305B

4. Install the front cover.

3028B 4. Refer to the instruction.
306B

5. Install the reagent door.

3029B 5. Refer to the instruction.
307B

6. Install the fluidic door.

30B 6. Refer to the instruction.
308B

7. Connect the power supply and switch on

301B 7. Refer to the instruction
309B

the instrument.
8. Verify the good working in check
302B

sensor screen.

Page 220/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Discharge Tubing Disassembly

Discharge Tubing replacement.

37B

Tools/Material
304B
• Cutting pliers

DISMANTLING
3041B

Action
3042B Step
304B Reference
304B

Prerequisite
3045B 1.
3046B Switch off the instrument and disconnect 1.
3051B Refer to the instruction.
the power supply.
2.
3047B Remove the fluidic door. 2.
3052B Refer to the instruction.
3.
3048B Remove the reagent door. 3.
305B Refer to the instruction.
4.
3049B Remove the top cover. 4.
3054B Refer to the instruction.
5.
305B Remove the reagent plate. 5.
305B Refer to the instruction.

Dismantling
3056B 1.
3057B Locate the discharge tubing at the back of
the unit, fixed with 2 fasteners on the
vacuum tank support.

Dismantling
3058B 2.
3059B Remove the 2 fasteners.

Page 221/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Dismantling
306B 3.
3061B Disconnect the discharge tubing from
the stopcock.

Dismantling
3062B 4.
306B Disconnect the discharge tubing from the
Y
connector, then remove it from the
3064B

unit.

Page 222/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Discharge Tubing assembling

374B

Tools/Material
3065B • NA

ASSEMBLING
306B

Action
3067B Step
3068B Reference
3069B

Prerequisite
307B
1.
3071B Follow the instruction of Discharge 1.
3072B Refer to the instruction.
Tubing dismantling.

Assembling
307B 5.
3074B Install the discharge tubing on the
vacuum tank support with the 2
fasteners.

Assembling
3075B 6.
3076B Connect the discharge tubing on the Y
connector.
307B

Page 223/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Assembling
3078B 7.
3079B Connect the discharge tubing on
the stopcock.

Completion
308B 1. Install the reagent plate.
3081B 1. Refer to the instruction.
308B

2. Install the top cover.

3082B 2. Refer to the instruction.
3089B

3. Install the reagent door.

308B 3. Refer to the instruction.
309B

4. Install the fluidic door.

3084B 4. Refer to the instruction.
3091B

5. Connect the power supply and switch on

3085B 5. Refer to the instruction.
3092B

the instrument.
6. Select REAGENT/PRIME LYSE.
3086B 6.
309B Refer to the instruction
7. Perform a startup cycle.
3087B
7.
3094B Refer to the instruction

Page 224/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

10. TROUBLESHOOTING

In any case, if a problem is not solved, contact Orphee service department.

3095B

10.1 Analytical problems

Parameters Problems Solutions

No HGB test results Check HGB LED wires Check/change HGB LED
Check/change HGB detection board
Check/change CPU board

HGB test result is OK while 1) Check the electrode wires.

no other parameters’ test 2) Firstly perform a Clean cycle. If the problem is
results. still unresolved, then perform a Bleach Cleaning
cycle. If still the same, check the good
connection of ground cable; If still the same,
change WBC aperture (counting head); If still
the same, change CPU board
Bad stability 1) Firstly perform a Backflush cycle. If the
problem is still unresolved, then perform a Clean
WBC
cycle. If the problem is still unresolved,
lastly perform a Bleach Cleaning cycle
2) Check if there are bubbles in WBC bath
during the analysis cycle. Check for good
sampling; Check, for good dilution; Check for
good bubbling (mixing); Check for good draining.

No HCT or PLT test results 1) Check the electrode wires.

2) Firstly perform a Clean cycle. If the problem is
still unresolved, then perform a Bleach Cleaning
RBC cycle. If still the same, check the good
connection of ground cable ; If still the same,
change RBC aperture (counting head); If still the
same, change CPU board.

Page 225/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

No HCT or PLT test results; 1) Firstly perform a Backflush cycle. If the

Bad stability problem is still unresolved, then perform a Clean
cycle. If the problem is still unresolved,
lastly perform a Bleach Cleaning cycle
2) Check if there are bubbles in RBC bath
during the analysis cycle.
3) Check the level of bubbles in WBC bath
during the first dilution. Check for good
sampling; Check, for good dilution; Check for
good bubbling (mixing); Check for good draining

No results 1) Check if the HGB LED light is on.

2) Check/change HGB LED

3) Check/change HGB detection board

4) Check/change CPU board

HGB Bad stability 1) Check the level bubble flow in the WBC
bath during the run cycle.
2) Change Lyse reagent
3) Verify the fluidic line for lyse
4) Check if there is bubbles in WBC chamber during
HGB measurement.
Rejection Perform a new Start Up cycle.

10.2 Mechanical Problems Troubleshooting

Problems Solutions
1) Check the rinsing needle block (presence of clots) and clean it
if necessary
2) Check the rinsing head.
Diluent leaks around 3) Check if needle is bent
the needle during 4) Check for pinched/pierced tubing
sample tests 5) Check valves 12,
6) Verify the good tightening of needle guide
7) Change rinse head O-ring
1) Check the power supply connection wires.
Fail to turn on the 2) Check the Power Supply block
analyzer 3) Check fuse on CPU board

Page 226/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

1) Check whether diluent container is placed at the same level

with the analyzer and whether the diluent tubing is not pinched.
2) Check diluent pick up tubing connection (untighten)
All test results are 3) Check for waste tubing pinched
bad. 4) Check valve 7
5) Check for valve 17
6) Check the diluent’s remaining volume.

No test results are Check the screen wires which are connected to CPU board.
displayed
1) Check the paper.
No printing 2) Check the electrical connection.

10.3 Troubleshooting message

This section allows to know what to do when a troubleshooting message appears on the screen.
3569B

Prompt Trigger Consequenc Troubleshooting

Cycle stopped upon
user request 1) Press on the pop-out.
Cycle stopped by (emergency stop Cycle is
user button). stopped. 2) Perform Fluidics Control
Cycle.

1) Press on the pop-out.

2) Check presence of
diluent inside WBC bath.
3) Check all connections
Startup failed-HGB The operator 4) Perform HGB
Adj. Failed STARTUP cycle is can run Sample adjustment
failed but HGB is 5) Check HGB LED (change
invalidated is necessary)
6) Check HGB board
(change is necessary)
7) Check CPU board
(change if necessary)

1) Press on the pop-out.

Startup STARTUP cycle is the operator 2) Perform a second

failed-Background failed can run Sample startup
Check Failed. 3) Perform bleach cycle
4) Troubleshoot on the
concerned parameter

Page 227/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Trying to run a new 1) Press on the pop-out.

cycle while another A new cycle
CYCLE BUSY
cycle is running. cannot be run. 2) Wait the end of the
cycle in progress then retry

Fluidic control cycle is 1) Press on the pop-out.

FLUIDICS CONTROL not run after an A new cycle
CYCLE NOT DONE emergency stop. cannot be run. 2) perform FLUIDICS
CONTROL cycle

user attempts to run

a cycle except
PRIME ALL CYCLE Startup after a clean 1) Press on the pop-out.
NOT DONE out without Analysis cycle
performing a Prime is not allowed. 2) Run PRIME ALL cycle
ALL

STARTUP NOT STARTUP cycle is Analysis cycle 1) Press on the pop-out.

DONE not done is not allowed.
2) Run STARTUP cycle

User attempts to run

a cycle after a drain
RINSE CYCLE NOT or a drain flow cell 1) Press on the pop-out.
DONE without performing a Analysis cycle
rinse cycle. is not allowed. 2) Run RINSE cycle

Users try to perform

other operations
except startup while
the system All operations 1) Press on the pop-out.
MOTOR INIT NOT initialization is not except startup
DONE run after an are not allowed. 2) Perform a motor
emergency stop initialization

Fluidic door is opened

during an ongoing
cycle except when the The ongoing 1) Press on the pop-out.
FLUIDIC DOOR
user is asked for cycle will be
OPENED
stopped. 2) Close FLUIDIC DOOR

Fluidic door is open

PLEASE CLOSE and/or is required to N/A 1) Press on the pop-out.
FLUIDIC DOOR TO be closed to run the 2) Close FLUIDIC DOOR
CONTINUE cycle

Page 228/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

When fluidic door is N/A 1) Press on the pop-out.

still not closed after
CYCLE AUTO “PLEASE CLOSE 2) Close FLUIDIC DOOR
STOPPED FLUIDIC DOOR TO
CONTINUE” occurred
once

1) Press on the pop-out.

When the measured 2) Check the presence of

pressure is out of the diluent in rinse head fluidic
range defined in the circuit
fluidic cycle, an error 3) Check if tub clogged or
NEEDLE RINSING is raised and the pinched in rinse head fluidic
DEFAULT current cycle is circuit
stopped. Emergency stop 4) Check the rinse pump
Vacuum failure during is initiated. 5) Check the inline
the needle rinsing pressure sensor
6) Check valves
#3,4,5,6,18,12 in check sensors
screen

1) Press on the pop-out.

2) Check the presence of

reagent in counting bath
3) Check the CPU pressure
sensor
4) Check if tub clogged or
DRAIN BATH
pinched in drain bath fluidic
DEFAULT
circuit
5) Check valve #1 or 2
Vacuum failure during Emergency stop depending from the bath
bath draining in is initiated. concerned
analysis cycle 6) Check valve #17 and 8
(atmosphere circuit)
7) Check for leak on waste
syringes
Vacuum failure during
counting phase in Emergency stop 1) Press on
analysis cycle is initiated.
VACUUM COUNTING
2) Check the CPU pressure
DEFAULT
sensor
3) Check for leak on waste
syringes
4) Check syringe module

Vacuum failure at 1) Press on

NO DILUENT begin of bath draining Emergency
stop. 2) Replace the empty
diluent container by a new one

Page 229/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

1) Press on

2) Check for leak on waste

SYRINGE VACUUM syringes
DEFAULT Vacuum failure during 3) Check for disconnected
test syringe cycle. Emergency tubing
stop. 4) Check the CPU pressure
sensor
5) Check syringe module

1) Press on

2) Check valve #7
WASTE DRAINING Pressure failure 3) Check for pinched
DEFAULT during test syringe tubing on waste line
cycle (on draining Emergency 4) Check syringe module
waste from syringe) stop. 5) Check the CPU pressure
sensor (Change CPU as
necessary)

1) Press on

2) Check for pinched or

clogged tubing on pump line
PUMP DEFAULT 3) Check valve #3 (change
as necessary)
Pressure failure Emergency 4) Check in line sensor
during pump test stop. (change as necessary)
5) Change the pump
(change as necessary)

1) Press on
NO BLEACH IN Detected air on Emergency
THE BATH bleach cycle. stop. 2) Redo bleach cycle
3) Check for leakage
Vacuum stability Emergency stop.
check failed during
syringe vacuum 1) Press on
SYRINGE VACUUM
2) Check for leak on waste syringes
STABILITY
3) Check for disconnected tubing
DEFAULT
4) Check the CPU pressure sensor
5) Check syringe module using CHECK
SYRINGE option

Page 230/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

1) Press on

ACUUM COUNTING 2) Check the CPU pressure

STABILITY sensor
Vacuum stability 3) Check for leak on waste
DEFAULT
check failed during Emergency syringes
counting vacuum stop. 4) Check syringe module
using CHECK SYRINGE option

1) Press on

2) Check the presence of

diluent in rinse head fluidic
circuit
3) Check for tubing
clogged/pinched in rinse head
NEEDLE RINSING
fluidic circuit
DEFAULT
4) Check the rinse pump
USING TEST PUMP option
5) Check the inline
Vacuum stability Emergency pressure sensor USING TEST
failed during the stop. PUMP option
needle rinsing. 6) Check valves
#3,4,5,6,18,12 in check sensors
screen

MOTOR INIT NOT Motors initialization Emergency 1) Press on

DONE is not done. stop.
2) Perform SYSTEM INIT

1) Press on
Steps lost on the Emergency
needle motor. stop. 2) Check for mechanical
NEEDLE GAP
hard point on needle movement
3) Check Needle using
CHECK NEEDLE option
4) Check Needle sensor
CHECK NEEDLE option

1) Press on

2) Check Needle using

NEEDLE HOME NOT Needle cannot join CHECK NEEDLE option
FOUND its home Emergency 3) Check Needle motor
stop. using
CHECK NEEDLE option
4) Check CPU (change as
necessary)

Page 231/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

1) Press on

Needle not in-home 2) Check Needle sensor

NEEDLE HOME NOT position detected using CHECK NEEDLE option
FOUND before to perform Emergency 3) Check needle sensor
cycle. stop. using
CHECK NEEDLE option
4) Check CPU

1) Press on

NEEDLE HOME Home needle Emergency 2) Check needle sensor

ERROR detection error stop. using
CHECK NEEDLE option
3) Check CPU

1) Press on

ROCKER GAP 2) Check Rocker module

using CHECK ROCKER option
Steps loss on the Emergency 3) Check Rocker sensor
rocker motor. stop. using CHECK ROCKER option

1) Press on

ROCKER HOME 2) Check Rocker sensor

ERROR Home rocker Emergency using CHECK ROCKER option
detection error stop. 3) Check CPU (change as
necessary)

Home rocker cannot Emergency

join its home stop. 1) Press on
2) Check Rocker sensor
using CHECK ROCKER option
3) Check Rocker module
ROCKER HOME NOT using CHECK ROCKER option
FOUND 4) Check CPU (change as
necessary)

Page 232/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

1) Press on

SYRINGE HOME 2) Check syringe sensor

ERROR Home Syringe Emergency using CHECK SYRINGE option
detection error stop. 3) Check CPU (change as
necessary)

1) Press on

2) Check Syringe sensor

SYRINGE HOME using CHECK SYRINGE option
NOT FOUND 3) Check Syringe module
using CHECK SYRINGE option
Home Syringe cannot Emergency 4) Check CPU (change as
join its home stop. necessary)

1) Press on

SYRINGE GAP 2) Check Syringe module

using CHECK SYRINGE option
Steps loss on the Emergency 3) Check Syringe sensor
syringe motor. stop. using CHECK SYRINGE option

1) Press on

2) Reboot the automaton

The system detected 3) Check HGB and
communication error FSC/ALL
with satellite EMERGENCY boards connection.
BOARDS board(s). STOP 4) Check HGB and
COMMUNICATION FSC/ALL
ERROR cable
5) If one of the cable
above was disconnected,
please connect the cable to the
board and reboot the
instrument
6) Check CPU board.

Page 233/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

1) Press on

2) Verify connections on
valve
EV COMMAND 3) Verify electrical
ERROR Valve] is not continuity on
connected (plugged or EMERGENCY 4) Replace valve
out of order) or is STOP 5) Check fuse (change as
short-circuited necessary)
6) Check CPU board
(change as necessary)
A prompt
informs the 1) Press on
user: Reagent
MAXIMUM REAGENT
temperature 2) Check cable connections
TEMPERATURE
over 60. 3) Check the heater
REACHED. HEATER
Heating is (change as necessary)
IS STOPPED.
Reagent stopped. Note: Instrument must be
temperature > 60°C Run sample restarted after issue fixing to
inaccessible. unlock the error
Heating is
stopped; A 1) Press on
prompt informs
Reagent heating at the user: 2) Check cable connections
REAGENTS HEATER 100% and no Reagent 3) Check the heater
IS STOPPED temperature increase heating failed. 4) Check CPU
during 2mn (less than Run sample Note: Instrument must be
0.5 increase) inaccessible. restarted after issue fixing to
unlock the error
Run sample
inaccessible 1) Press on
(except with
SERVICE or 2) Wait for set
higher rights). temperature reached
REAGENT These
TEMPERATURE IS If the reagent conditions
OUT OF RANGE. temperature is < to disable the
ANALYSIS ARE NOT target – 2.5°C probe down
ALLOWED function.
The error
prompt appears
when user goes
in screen where
you can run
sample.

Page 234/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

1) Press on

INSTRUMENT If the instrument A prompt 2) Wait for set

TEMPERATURE OUT temperature is < to informs the temperature reached
OF RANGE. 18°C or > to 36.5°C user 3) Check cable connections
4) Check the heater
5) Check CPU

No cycle can be
PLEASE CHANGE launched 1) Press on
LYSE: CONTAINER (except in R&D
IS EMPTY If Lyse is empty Mode) 2) Change the lyse bottle
3) Perform lyse priming
No cycle can be
PLEASE CHANGE launched 1) Press on
CLEANER: (except in R&D
CONTAINER IS If Cleaner is empty Mode) 2) Change the cleaner
EMPTY bottle
3) Perform cleaner priming

PLEASE CHANGE No cycle can be 1) Press on

DILUENT: launched
CONTAINER IS If Diluent is empty (except in R&D 2) Change the diluent
EMPTY Mode) container
3) Perform diluent priming

PLEASE REPLACE No cycle can be 1) Press on

DILUENT launched
CONTAINER: Diluent Expiration (except in R&D 2) Change the diluent
REAGENT IS OUT date is attempted Mode) container
OF DATE
3) Perform diluent priming

PLEASE REPLACE no cycle can be

CLEANER launched 1) Press on
CONTAINER: Cleaner Expiration (except in
REAGENT IS OUT date is attempted R&D Mode) 2) Change the lyse bottle
OF DATE
3) Perform lyse priming
no cycle can be
PLEASE REPLACE launched 1) Press on
LYSE CONTAINER: Lyse Expiration date (except in
REAGENT IS OUT is attempted R&D Mode) 2) Change the lyse bottle
OF DATE
3) Perform lyse priming

Page 235/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

no cycle can be PLEASE

launched (except in REPLACE 1) Press on
R&D Mode) LYSE 2) Change the lyse bottle
Lyse Open date is
CONTAINER: 3) Perform lyse priming
exceeded
REAGENT IS
OUT OF
DATE

PLEASE REPLACE no cycle can be 1) Press on

DILUENT launched
CONTAINER: Diluent Open date is (except 2) Change the diluent
REAGENT IS OUT exceeded in R&D container
OF DATE Mode) 3) Perform diluent priming

PLEASE REPLACE no cycle can be

CLEANER launched 1) Press on
CONTAINER: Cleaner Open date is (except in R&D
REAGENT IS OUT exceeded Mode) 2) Change the cleaner
OF DATE bottle
3) Perform cleaner priming

REAGENT 1) Press on
AUTENTIFICATION
FAILED Incorrect code entry NA 2) Enter the correct code
then validate
Reagent lot (lot
number + bottle
REAGENT ALREADY SERIAL) already 1) Press on
USED exists in the reagent NA
log 2) Change the diluent data
3) Perform diluent priming

1) Press on
LOT NUMBER IS Invalid Lot Number
NOT FOR LYSE for lyse NA 2) Enter the correct
number then validate

1) Press on
LOT NUMBER IS Invalid Lot Number
NOT FOR DILUENT for diluent NA 2) Enter the correct
number then validate

1) Press on
LOT NUMBER IS Invalid Lot Number
NOT FOR CLEANER for cleaner NA 2) Enter the correct
number then validate

Page 236/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

The user attempt to

MAXIMUM NUMBER register more than 12 1) Press on
OF USERS IS users NA
REACHED
2) Suppress one or few
users
An error occurred
during software N/A 1) Press on
upgrade 2) Try again
SOFTWARE 3) Check USB key (change
UPGRADE ERROR as necessary)
4) Contact Customer
Service
Department
ACTION ON
The user attempts to THE 1) Press on
manually activate/de- ACTIONER IS
CYCLE BUSY
active an actioner NOT 2) Wait the end of the
PERFORMED cycle in progress then retry

1) Press on

2) Try again
3) Perform a bleach cycle
RBC RESISTIVE 4) Check the latex solution
GAIN ADJUSTMENT Resistive gain (polluted)
FAILED adjustment failed Default gain is 5) Check counting head
applied 6) Check cable and
connections
7) Check CPU (change as
necessary)

1) Press on

2) Try again
3) Perform a bleach cycle
WBC RESISTIVE 4) Check the latex solution
GAIN ADJUSTMENT (polluted)
FAILED Resistive gain Default gain is 5) Check counting head
adjustment failed applied 6) Check cable and
connections
7) Check CPU (change as
necessary)

Page 237/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

1) Press on

The HGB 2) Proceed to HGB LED

HBG LED invalidated until adjustment again
ADJUSTMENT an adjustment 3) Verify the presence of
FAILED The adjustment of the LED diluent in WBC bath
of the HGB successes. 4) Check cable and
LED failed. connections
5) Check HGB LED
6) Check HGB board

1) Press on
The DIF
Measurement is 2) Proceed to OPT LED
OPT LED invalidated until adjustment again
ADJUSTEMENT The adjustment of an adjustment 3) Verify the presence of
FAILED the OPT LED is failed. of the LED diluent in WBC bath
successes. 4) Check cable and
connections
5) Check optical bench

If more than one 1) Press on

sequence has a
GAIN ADJUSTMENT reference out of the 2) Try again
FAILED fixed range N/A 3) Check cable and
(Target connections
±10%) 4) Check optical bench

1) Press on

2) Check for tubing

pinched or clogged
GAIN ADJUSTMENT 3) Check for cables
FAILED connections
if pulse count in the 4) Check for optic nozzle
area is not sufficient N/A clogged
5) Change latex solution
6) Check for CPU (change
as necessary)

Page 238/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60

 REF : M60-SM-000 Rev 02

Page 239/239 Copyright© Orphée SA. All Rights Reserved MYTHIC 60